Activation and cytokine profile of monocyte derived dendritic cells in leprosy: in vitro stimulation by sonicated Mycobacterium leprae induces decreased level of IL-12p70 in lepromatous leprosy

Detalhes bibliográficos
Autor(a) principal: Braga, Andre Flores [UNESP]
Data de Publicação: 2015
Outros Autores: Moretto, Daniela Ferraz, Gigliotti, Patricia [UNESP], Peruchi, Mariela, Vilani-Moreno, Fatima Regina, Campanelli, Ana Paula, Pereira Latini, Ana Carla, Iyer, Anand, Das, Pranab Kumar, Brito de Souza, Vania Nieto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/0074-02760140230
http://hdl.handle.net/11449/160764
Resumo: Dendritic cells (DCs) play a pivotal role in the connection of innate and adaptive immunity of hosts to mycobacterial infection. Studies on the interaction of monocyte-derived DCs (MO-DCs) using Mycobacterium leprae in leprosy patients are rare. The present study demonstrated that the differentiation of MOs to DCs was similar in all forms of leprosy compared to normal healthy individuals. In vitro stimulation of immature MO-DCs with sonicated M. leprae induced variable degrees of DC maturation as determined by the increased expression of HLA-DR, CD40, CD80 and CD86, but not CD83, in all studied groups. The production of different cytokines by the MO-DCs appeared similar in all of the studied groups under similar conditions. However, the production of interleukin (IL)-12p70 by MO-DCs from lepromatous (LL) leprosy patients after in vitro stimulation with M. leprae was lower than tuberculoid leprosy patients and healthy individuals, even after CD40 ligation with CD40 ligand-transfected cells. The present cumulative findings suggest that the MO-DCs of LL patients are generally a weak producer of IL-12p70 despite the moderate activating properties of M. leprae. These results may explain the poor M. leprae-specific cell-mediated immunity in the LL type of leprosy.
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spelling Activation and cytokine profile of monocyte derived dendritic cells in leprosy: in vitro stimulation by sonicated Mycobacterium leprae induces decreased level of IL-12p70 in lepromatous leprosyleprosydendritic cellsIL-12p70Dendritic cells (DCs) play a pivotal role in the connection of innate and adaptive immunity of hosts to mycobacterial infection. Studies on the interaction of monocyte-derived DCs (MO-DCs) using Mycobacterium leprae in leprosy patients are rare. The present study demonstrated that the differentiation of MOs to DCs was similar in all forms of leprosy compared to normal healthy individuals. In vitro stimulation of immature MO-DCs with sonicated M. leprae induced variable degrees of DC maturation as determined by the increased expression of HLA-DR, CD40, CD80 and CD86, but not CD83, in all studied groups. The production of different cytokines by the MO-DCs appeared similar in all of the studied groups under similar conditions. However, the production of interleukin (IL)-12p70 by MO-DCs from lepromatous (LL) leprosy patients after in vitro stimulation with M. leprae was lower than tuberculoid leprosy patients and healthy individuals, even after CD40 ligation with CD40 ligand-transfected cells. The present cumulative findings suggest that the MO-DCs of LL patients are generally a weak producer of IL-12p70 despite the moderate activating properties of M. leprae. These results may explain the poor M. leprae-specific cell-mediated immunity in the LL type of leprosy.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)AMC-UvAUniv Estadual Paulista, Fac Med Botucatu, Programa Posgrad Doencas Trop, Botucatu, SP, BrazilUniv Santo Amaro, Sao Paulo, SP, BrazilFac Anhanguera Bauru, Bauru, SP, BrazilInst Lauro Souza Lima, Equipe Tecn Imunol, Bauru, SP, BrazilUniv Sao Paulo, Fac Odontol Bauru, Dept Microbiol & Imunol, Bauru, SP, BrazilUniv Amsterdam, Acad Med Ctr, Dept Pathol, NL-1105 AZ Amsterdam, North Holland, NetherlandsUniv Birmingham, Sch Immun & Infect, Birmingham, W Midlands, EnglandUniv Estadual Paulista, Fac Med Botucatu, Programa Posgrad Doencas Trop, Botucatu, SP, BrazilFAPESP: 2009/01436-1Fundaco Oswaldo CruzUniversidade Estadual Paulista (Unesp)Univ Santo AmaroFac Anhanguera BauruInst Lauro Souza LimaUniversidade de São Paulo (USP)Univ AmsterdamUniv BirminghamBraga, Andre Flores [UNESP]Moretto, Daniela FerrazGigliotti, Patricia [UNESP]Peruchi, MarielaVilani-Moreno, Fatima ReginaCampanelli, Ana PaulaPereira Latini, Ana CarlaIyer, AnandDas, Pranab KumarBrito de Souza, Vania Nieto2018-11-26T16:16:37Z2018-11-26T16:16:37Z2015-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article655-661application/pdfhttp://dx.doi.org/10.1590/0074-02760140230Memorias Do Instituto Oswaldo Cruz. Rio De Janeiro, Rj: Fundaco Oswaldo Cruz, v. 110, n. 5, p. 655-661, 2015.0074-0276http://hdl.handle.net/11449/16076410.1590/0074-02760140230S0074-02762015000500655WOS:000360504200012S0074-02762015000500655.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemorias Do Instituto Oswaldo Cruz1,172info:eu-repo/semantics/openAccess2024-08-15T15:23:26Zoai:repositorio.unesp.br:11449/160764Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-15T15:23:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Activation and cytokine profile of monocyte derived dendritic cells in leprosy: in vitro stimulation by sonicated Mycobacterium leprae induces decreased level of IL-12p70 in lepromatous leprosy
title Activation and cytokine profile of monocyte derived dendritic cells in leprosy: in vitro stimulation by sonicated Mycobacterium leprae induces decreased level of IL-12p70 in lepromatous leprosy
spellingShingle Activation and cytokine profile of monocyte derived dendritic cells in leprosy: in vitro stimulation by sonicated Mycobacterium leprae induces decreased level of IL-12p70 in lepromatous leprosy
Braga, Andre Flores [UNESP]
leprosy
dendritic cells
IL-12p70
title_short Activation and cytokine profile of monocyte derived dendritic cells in leprosy: in vitro stimulation by sonicated Mycobacterium leprae induces decreased level of IL-12p70 in lepromatous leprosy
title_full Activation and cytokine profile of monocyte derived dendritic cells in leprosy: in vitro stimulation by sonicated Mycobacterium leprae induces decreased level of IL-12p70 in lepromatous leprosy
title_fullStr Activation and cytokine profile of monocyte derived dendritic cells in leprosy: in vitro stimulation by sonicated Mycobacterium leprae induces decreased level of IL-12p70 in lepromatous leprosy
title_full_unstemmed Activation and cytokine profile of monocyte derived dendritic cells in leprosy: in vitro stimulation by sonicated Mycobacterium leprae induces decreased level of IL-12p70 in lepromatous leprosy
title_sort Activation and cytokine profile of monocyte derived dendritic cells in leprosy: in vitro stimulation by sonicated Mycobacterium leprae induces decreased level of IL-12p70 in lepromatous leprosy
author Braga, Andre Flores [UNESP]
author_facet Braga, Andre Flores [UNESP]
Moretto, Daniela Ferraz
Gigliotti, Patricia [UNESP]
Peruchi, Mariela
Vilani-Moreno, Fatima Regina
Campanelli, Ana Paula
Pereira Latini, Ana Carla
Iyer, Anand
Das, Pranab Kumar
Brito de Souza, Vania Nieto
author_role author
author2 Moretto, Daniela Ferraz
Gigliotti, Patricia [UNESP]
Peruchi, Mariela
Vilani-Moreno, Fatima Regina
Campanelli, Ana Paula
Pereira Latini, Ana Carla
Iyer, Anand
Das, Pranab Kumar
Brito de Souza, Vania Nieto
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Univ Santo Amaro
Fac Anhanguera Bauru
Inst Lauro Souza Lima
Universidade de São Paulo (USP)
Univ Amsterdam
Univ Birmingham
dc.contributor.author.fl_str_mv Braga, Andre Flores [UNESP]
Moretto, Daniela Ferraz
Gigliotti, Patricia [UNESP]
Peruchi, Mariela
Vilani-Moreno, Fatima Regina
Campanelli, Ana Paula
Pereira Latini, Ana Carla
Iyer, Anand
Das, Pranab Kumar
Brito de Souza, Vania Nieto
dc.subject.por.fl_str_mv leprosy
dendritic cells
IL-12p70
topic leprosy
dendritic cells
IL-12p70
description Dendritic cells (DCs) play a pivotal role in the connection of innate and adaptive immunity of hosts to mycobacterial infection. Studies on the interaction of monocyte-derived DCs (MO-DCs) using Mycobacterium leprae in leprosy patients are rare. The present study demonstrated that the differentiation of MOs to DCs was similar in all forms of leprosy compared to normal healthy individuals. In vitro stimulation of immature MO-DCs with sonicated M. leprae induced variable degrees of DC maturation as determined by the increased expression of HLA-DR, CD40, CD80 and CD86, but not CD83, in all studied groups. The production of different cytokines by the MO-DCs appeared similar in all of the studied groups under similar conditions. However, the production of interleukin (IL)-12p70 by MO-DCs from lepromatous (LL) leprosy patients after in vitro stimulation with M. leprae was lower than tuberculoid leprosy patients and healthy individuals, even after CD40 ligation with CD40 ligand-transfected cells. The present cumulative findings suggest that the MO-DCs of LL patients are generally a weak producer of IL-12p70 despite the moderate activating properties of M. leprae. These results may explain the poor M. leprae-specific cell-mediated immunity in the LL type of leprosy.
publishDate 2015
dc.date.none.fl_str_mv 2015-08-01
2018-11-26T16:16:37Z
2018-11-26T16:16:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/0074-02760140230
Memorias Do Instituto Oswaldo Cruz. Rio De Janeiro, Rj: Fundaco Oswaldo Cruz, v. 110, n. 5, p. 655-661, 2015.
0074-0276
http://hdl.handle.net/11449/160764
10.1590/0074-02760140230
S0074-02762015000500655
WOS:000360504200012
S0074-02762015000500655.pdf
url http://dx.doi.org/10.1590/0074-02760140230
http://hdl.handle.net/11449/160764
identifier_str_mv Memorias Do Instituto Oswaldo Cruz. Rio De Janeiro, Rj: Fundaco Oswaldo Cruz, v. 110, n. 5, p. 655-661, 2015.
0074-0276
10.1590/0074-02760140230
S0074-02762015000500655
WOS:000360504200012
S0074-02762015000500655.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Memorias Do Instituto Oswaldo Cruz
1,172
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 655-661
application/pdf
dc.publisher.none.fl_str_mv Fundaco Oswaldo Cruz
publisher.none.fl_str_mv Fundaco Oswaldo Cruz
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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