Activation and cytokine profile of monocyte derived dendritic cells in leprosy: in vitro stimulation by sonicated Mycobacterium leprae induces decreased level of IL-12p70 in lepromatous leprosy
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0074-02760140230 http://hdl.handle.net/11449/160764 |
Resumo: | Dendritic cells (DCs) play a pivotal role in the connection of innate and adaptive immunity of hosts to mycobacterial infection. Studies on the interaction of monocyte-derived DCs (MO-DCs) using Mycobacterium leprae in leprosy patients are rare. The present study demonstrated that the differentiation of MOs to DCs was similar in all forms of leprosy compared to normal healthy individuals. In vitro stimulation of immature MO-DCs with sonicated M. leprae induced variable degrees of DC maturation as determined by the increased expression of HLA-DR, CD40, CD80 and CD86, but not CD83, in all studied groups. The production of different cytokines by the MO-DCs appeared similar in all of the studied groups under similar conditions. However, the production of interleukin (IL)-12p70 by MO-DCs from lepromatous (LL) leprosy patients after in vitro stimulation with M. leprae was lower than tuberculoid leprosy patients and healthy individuals, even after CD40 ligation with CD40 ligand-transfected cells. The present cumulative findings suggest that the MO-DCs of LL patients are generally a weak producer of IL-12p70 despite the moderate activating properties of M. leprae. These results may explain the poor M. leprae-specific cell-mediated immunity in the LL type of leprosy. |
id |
UNSP_1f637c554ed25860dd5b00ba20ebdb72 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/160764 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Activation and cytokine profile of monocyte derived dendritic cells in leprosy: in vitro stimulation by sonicated Mycobacterium leprae induces decreased level of IL-12p70 in lepromatous leprosyleprosydendritic cellsIL-12p70Dendritic cells (DCs) play a pivotal role in the connection of innate and adaptive immunity of hosts to mycobacterial infection. Studies on the interaction of monocyte-derived DCs (MO-DCs) using Mycobacterium leprae in leprosy patients are rare. The present study demonstrated that the differentiation of MOs to DCs was similar in all forms of leprosy compared to normal healthy individuals. In vitro stimulation of immature MO-DCs with sonicated M. leprae induced variable degrees of DC maturation as determined by the increased expression of HLA-DR, CD40, CD80 and CD86, but not CD83, in all studied groups. The production of different cytokines by the MO-DCs appeared similar in all of the studied groups under similar conditions. However, the production of interleukin (IL)-12p70 by MO-DCs from lepromatous (LL) leprosy patients after in vitro stimulation with M. leprae was lower than tuberculoid leprosy patients and healthy individuals, even after CD40 ligation with CD40 ligand-transfected cells. The present cumulative findings suggest that the MO-DCs of LL patients are generally a weak producer of IL-12p70 despite the moderate activating properties of M. leprae. These results may explain the poor M. leprae-specific cell-mediated immunity in the LL type of leprosy.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)AMC-UvAUniv Estadual Paulista, Fac Med Botucatu, Programa Posgrad Doencas Trop, Botucatu, SP, BrazilUniv Santo Amaro, Sao Paulo, SP, BrazilFac Anhanguera Bauru, Bauru, SP, BrazilInst Lauro Souza Lima, Equipe Tecn Imunol, Bauru, SP, BrazilUniv Sao Paulo, Fac Odontol Bauru, Dept Microbiol & Imunol, Bauru, SP, BrazilUniv Amsterdam, Acad Med Ctr, Dept Pathol, NL-1105 AZ Amsterdam, North Holland, NetherlandsUniv Birmingham, Sch Immun & Infect, Birmingham, W Midlands, EnglandUniv Estadual Paulista, Fac Med Botucatu, Programa Posgrad Doencas Trop, Botucatu, SP, BrazilFAPESP: 2009/01436-1Fundaco Oswaldo CruzUniversidade Estadual Paulista (Unesp)Univ Santo AmaroFac Anhanguera BauruInst Lauro Souza LimaUniversidade de São Paulo (USP)Univ AmsterdamUniv BirminghamBraga, Andre Flores [UNESP]Moretto, Daniela FerrazGigliotti, Patricia [UNESP]Peruchi, MarielaVilani-Moreno, Fatima ReginaCampanelli, Ana PaulaPereira Latini, Ana CarlaIyer, AnandDas, Pranab KumarBrito de Souza, Vania Nieto2018-11-26T16:16:37Z2018-11-26T16:16:37Z2015-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article655-661application/pdfhttp://dx.doi.org/10.1590/0074-02760140230Memorias Do Instituto Oswaldo Cruz. Rio De Janeiro, Rj: Fundaco Oswaldo Cruz, v. 110, n. 5, p. 655-661, 2015.0074-0276http://hdl.handle.net/11449/16076410.1590/0074-02760140230S0074-02762015000500655WOS:000360504200012S0074-02762015000500655.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemorias Do Instituto Oswaldo Cruz1,172info:eu-repo/semantics/openAccess2024-08-15T15:23:26Zoai:repositorio.unesp.br:11449/160764Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-15T15:23:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Activation and cytokine profile of monocyte derived dendritic cells in leprosy: in vitro stimulation by sonicated Mycobacterium leprae induces decreased level of IL-12p70 in lepromatous leprosy |
title |
Activation and cytokine profile of monocyte derived dendritic cells in leprosy: in vitro stimulation by sonicated Mycobacterium leprae induces decreased level of IL-12p70 in lepromatous leprosy |
spellingShingle |
Activation and cytokine profile of monocyte derived dendritic cells in leprosy: in vitro stimulation by sonicated Mycobacterium leprae induces decreased level of IL-12p70 in lepromatous leprosy Braga, Andre Flores [UNESP] leprosy dendritic cells IL-12p70 |
title_short |
Activation and cytokine profile of monocyte derived dendritic cells in leprosy: in vitro stimulation by sonicated Mycobacterium leprae induces decreased level of IL-12p70 in lepromatous leprosy |
title_full |
Activation and cytokine profile of monocyte derived dendritic cells in leprosy: in vitro stimulation by sonicated Mycobacterium leprae induces decreased level of IL-12p70 in lepromatous leprosy |
title_fullStr |
Activation and cytokine profile of monocyte derived dendritic cells in leprosy: in vitro stimulation by sonicated Mycobacterium leprae induces decreased level of IL-12p70 in lepromatous leprosy |
title_full_unstemmed |
Activation and cytokine profile of monocyte derived dendritic cells in leprosy: in vitro stimulation by sonicated Mycobacterium leprae induces decreased level of IL-12p70 in lepromatous leprosy |
title_sort |
Activation and cytokine profile of monocyte derived dendritic cells in leprosy: in vitro stimulation by sonicated Mycobacterium leprae induces decreased level of IL-12p70 in lepromatous leprosy |
author |
Braga, Andre Flores [UNESP] |
author_facet |
Braga, Andre Flores [UNESP] Moretto, Daniela Ferraz Gigliotti, Patricia [UNESP] Peruchi, Mariela Vilani-Moreno, Fatima Regina Campanelli, Ana Paula Pereira Latini, Ana Carla Iyer, Anand Das, Pranab Kumar Brito de Souza, Vania Nieto |
author_role |
author |
author2 |
Moretto, Daniela Ferraz Gigliotti, Patricia [UNESP] Peruchi, Mariela Vilani-Moreno, Fatima Regina Campanelli, Ana Paula Pereira Latini, Ana Carla Iyer, Anand Das, Pranab Kumar Brito de Souza, Vania Nieto |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Santo Amaro Fac Anhanguera Bauru Inst Lauro Souza Lima Universidade de São Paulo (USP) Univ Amsterdam Univ Birmingham |
dc.contributor.author.fl_str_mv |
Braga, Andre Flores [UNESP] Moretto, Daniela Ferraz Gigliotti, Patricia [UNESP] Peruchi, Mariela Vilani-Moreno, Fatima Regina Campanelli, Ana Paula Pereira Latini, Ana Carla Iyer, Anand Das, Pranab Kumar Brito de Souza, Vania Nieto |
dc.subject.por.fl_str_mv |
leprosy dendritic cells IL-12p70 |
topic |
leprosy dendritic cells IL-12p70 |
description |
Dendritic cells (DCs) play a pivotal role in the connection of innate and adaptive immunity of hosts to mycobacterial infection. Studies on the interaction of monocyte-derived DCs (MO-DCs) using Mycobacterium leprae in leprosy patients are rare. The present study demonstrated that the differentiation of MOs to DCs was similar in all forms of leprosy compared to normal healthy individuals. In vitro stimulation of immature MO-DCs with sonicated M. leprae induced variable degrees of DC maturation as determined by the increased expression of HLA-DR, CD40, CD80 and CD86, but not CD83, in all studied groups. The production of different cytokines by the MO-DCs appeared similar in all of the studied groups under similar conditions. However, the production of interleukin (IL)-12p70 by MO-DCs from lepromatous (LL) leprosy patients after in vitro stimulation with M. leprae was lower than tuberculoid leprosy patients and healthy individuals, even after CD40 ligation with CD40 ligand-transfected cells. The present cumulative findings suggest that the MO-DCs of LL patients are generally a weak producer of IL-12p70 despite the moderate activating properties of M. leprae. These results may explain the poor M. leprae-specific cell-mediated immunity in the LL type of leprosy. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-08-01 2018-11-26T16:16:37Z 2018-11-26T16:16:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0074-02760140230 Memorias Do Instituto Oswaldo Cruz. Rio De Janeiro, Rj: Fundaco Oswaldo Cruz, v. 110, n. 5, p. 655-661, 2015. 0074-0276 http://hdl.handle.net/11449/160764 10.1590/0074-02760140230 S0074-02762015000500655 WOS:000360504200012 S0074-02762015000500655.pdf |
url |
http://dx.doi.org/10.1590/0074-02760140230 http://hdl.handle.net/11449/160764 |
identifier_str_mv |
Memorias Do Instituto Oswaldo Cruz. Rio De Janeiro, Rj: Fundaco Oswaldo Cruz, v. 110, n. 5, p. 655-661, 2015. 0074-0276 10.1590/0074-02760140230 S0074-02762015000500655 WOS:000360504200012 S0074-02762015000500655.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Memorias Do Instituto Oswaldo Cruz 1,172 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
655-661 application/pdf |
dc.publisher.none.fl_str_mv |
Fundaco Oswaldo Cruz |
publisher.none.fl_str_mv |
Fundaco Oswaldo Cruz |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128199150796800 |