Improving enzyme production by solid-state cultivation in packed-bed bioreactors by changing bed porosity and airflow distribution
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00449-020-02466-7 http://hdl.handle.net/11449/208164 |
Resumo: | Enzymes production by solid-state cultivation in packed-bed bioreactor needs to be improved by mathematical modeling and also by experimentation. In this work, a mixture of sugarcane bagasse and wheat bran was used for the growth of the fungus Myceliophthora thermophila I-1D3b, able to secrete endoglucanase and xylanase, enzymes of interest in the second-generation ethanol production. Bench and pilot-scale bioreactors were used for the experiments, while critical parameters as bed porosity and airflow distribution were evaluated. Results showed enzymes with higher activities for the most porous medium, even though the less substrate amount to be cultivated. For the pilot-scale bioreactor, only the most porous medium was evaluated using different airflow distribution techniques. Using an inner tube for air supply resulted in more homogeneous enzyme production, with higher activities. The results here presented will be helpful for the scale-up of this class of bioreactor into industrial apparatuses. |
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Repositório Institucional da UNESP |
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Improving enzyme production by solid-state cultivation in packed-bed bioreactors by changing bed porosity and airflow distributionCellulaseFixed bioreactorPacking densityScale-upSolid-state fermentationEnzymes production by solid-state cultivation in packed-bed bioreactor needs to be improved by mathematical modeling and also by experimentation. In this work, a mixture of sugarcane bagasse and wheat bran was used for the growth of the fungus Myceliophthora thermophila I-1D3b, able to secrete endoglucanase and xylanase, enzymes of interest in the second-generation ethanol production. Bench and pilot-scale bioreactors were used for the experiments, while critical parameters as bed porosity and airflow distribution were evaluated. Results showed enzymes with higher activities for the most porous medium, even though the less substrate amount to be cultivated. For the pilot-scale bioreactor, only the most porous medium was evaluated using different airflow distribution techniques. Using an inner tube for air supply resulted in more homogeneous enzyme production, with higher activities. The results here presented will be helpful for the scale-up of this class of bioreactor into industrial apparatuses.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Food Engineering and Technology Department Institute of Biosciences Letters and Exact Sciences São Paulo State University (UNESP), Cristóvão Colombo, 2265, Jardim NazarethGraduate Program of Chemical Engineering Federal University of São Carlos (UFSCar), Rod. Washington Luiz km 235, SP-310Food Engineering and Technology Department Institute of Biosciences Letters and Exact Sciences São Paulo State University (UNESP), Cristóvão Colombo, 2265, Jardim NazarethCNPq: 132957/2016-7FAPESP: 2014/23453-3FAPESP: 2018/00996-2CNPq: 430786/2018-2Universidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Perez, Caroline Lopes [UNESP]Casciatori, Fernanda PerpétuaThoméo, João Cláudio [UNESP]2021-06-25T11:07:27Z2021-06-25T11:07:27Z2021-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article537-548http://dx.doi.org/10.1007/s00449-020-02466-7Bioprocess and Biosystems Engineering, v. 44, n. 3, p. 537-548, 2021.1615-76051615-7591http://hdl.handle.net/11449/20816410.1007/s00449-020-02466-72-s2.0-85096397336Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioprocess and Biosystems Engineeringinfo:eu-repo/semantics/openAccess2021-10-23T18:56:47Zoai:repositorio.unesp.br:11449/208164Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:43:14.438881Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Improving enzyme production by solid-state cultivation in packed-bed bioreactors by changing bed porosity and airflow distribution |
title |
Improving enzyme production by solid-state cultivation in packed-bed bioreactors by changing bed porosity and airflow distribution |
spellingShingle |
Improving enzyme production by solid-state cultivation in packed-bed bioreactors by changing bed porosity and airflow distribution Perez, Caroline Lopes [UNESP] Cellulase Fixed bioreactor Packing density Scale-up Solid-state fermentation |
title_short |
Improving enzyme production by solid-state cultivation in packed-bed bioreactors by changing bed porosity and airflow distribution |
title_full |
Improving enzyme production by solid-state cultivation in packed-bed bioreactors by changing bed porosity and airflow distribution |
title_fullStr |
Improving enzyme production by solid-state cultivation in packed-bed bioreactors by changing bed porosity and airflow distribution |
title_full_unstemmed |
Improving enzyme production by solid-state cultivation in packed-bed bioreactors by changing bed porosity and airflow distribution |
title_sort |
Improving enzyme production by solid-state cultivation in packed-bed bioreactors by changing bed porosity and airflow distribution |
author |
Perez, Caroline Lopes [UNESP] |
author_facet |
Perez, Caroline Lopes [UNESP] Casciatori, Fernanda Perpétua Thoméo, João Cláudio [UNESP] |
author_role |
author |
author2 |
Casciatori, Fernanda Perpétua Thoméo, João Cláudio [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Perez, Caroline Lopes [UNESP] Casciatori, Fernanda Perpétua Thoméo, João Cláudio [UNESP] |
dc.subject.por.fl_str_mv |
Cellulase Fixed bioreactor Packing density Scale-up Solid-state fermentation |
topic |
Cellulase Fixed bioreactor Packing density Scale-up Solid-state fermentation |
description |
Enzymes production by solid-state cultivation in packed-bed bioreactor needs to be improved by mathematical modeling and also by experimentation. In this work, a mixture of sugarcane bagasse and wheat bran was used for the growth of the fungus Myceliophthora thermophila I-1D3b, able to secrete endoglucanase and xylanase, enzymes of interest in the second-generation ethanol production. Bench and pilot-scale bioreactors were used for the experiments, while critical parameters as bed porosity and airflow distribution were evaluated. Results showed enzymes with higher activities for the most porous medium, even though the less substrate amount to be cultivated. For the pilot-scale bioreactor, only the most porous medium was evaluated using different airflow distribution techniques. Using an inner tube for air supply resulted in more homogeneous enzyme production, with higher activities. The results here presented will be helpful for the scale-up of this class of bioreactor into industrial apparatuses. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:07:27Z 2021-06-25T11:07:27Z 2021-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00449-020-02466-7 Bioprocess and Biosystems Engineering, v. 44, n. 3, p. 537-548, 2021. 1615-7605 1615-7591 http://hdl.handle.net/11449/208164 10.1007/s00449-020-02466-7 2-s2.0-85096397336 |
url |
http://dx.doi.org/10.1007/s00449-020-02466-7 http://hdl.handle.net/11449/208164 |
identifier_str_mv |
Bioprocess and Biosystems Engineering, v. 44, n. 3, p. 537-548, 2021. 1615-7605 1615-7591 10.1007/s00449-020-02466-7 2-s2.0-85096397336 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bioprocess and Biosystems Engineering |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
537-548 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128849644355584 |