GENE EXPRESSION OF FRESH AND FROZEN/THAWED CANINE EMBRYOS
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/209949 |
Resumo: | BACKGROUND: Canine embryo cryopreservation and subsequent transfer are relevant in the use of reproductive technologies. OBJECTIVE: The purpose of this study is the identification and quantification of the gene expression BAX and Bcl2, AQP3, Na+/K+ ATPase alpha 1 and beta 1 and LIFr in canine embryos obtained in vivo and after freezing. MATERIALS AND METHODS: For the collection of embryos, the bitches were identified at pro-estrous until the detection of 80-90% superficial cells. After that, they were artificially inseminated with fresh semen. The embryos were collected after ovariohysterectomy. RNA was extracted and amplified, and embryos were randomly distributed into fresh (Fr) and frozen/thawed (Ft) groups. RESULTS: Eighteen blastocysts were collected from three bitches. Genes BAX, AQP3 and LIFr did not differ among the studied groups. CONCLUSION: We suggest, through these results, that the genes BAX, Bcl2, AQP3, Na + / K + ATPase alpha 1 and beta 1 and LIFr were expressed in canine blastocysts collected in vivo and after slow freezing cryopreservation. |
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Repositório Institucional da UNESP |
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spelling |
GENE EXPRESSION OF FRESH AND FROZEN/THAWED CANINE EMBRYOSgenesembryosslow freezingBACKGROUND: Canine embryo cryopreservation and subsequent transfer are relevant in the use of reproductive technologies. OBJECTIVE: The purpose of this study is the identification and quantification of the gene expression BAX and Bcl2, AQP3, Na+/K+ ATPase alpha 1 and beta 1 and LIFr in canine embryos obtained in vivo and after freezing. MATERIALS AND METHODS: For the collection of embryos, the bitches were identified at pro-estrous until the detection of 80-90% superficial cells. After that, they were artificially inseminated with fresh semen. The embryos were collected after ovariohysterectomy. RNA was extracted and amplified, and embryos were randomly distributed into fresh (Fr) and frozen/thawed (Ft) groups. RESULTS: Eighteen blastocysts were collected from three bitches. Genes BAX, AQP3 and LIFr did not differ among the studied groups. CONCLUSION: We suggest, through these results, that the genes BAX, Bcl2, AQP3, Na + / K + ATPase alpha 1 and beta 1 and LIFr were expressed in canine blastocysts collected in vivo and after slow freezing cryopreservation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Paranaense, Umuarama, PR, BrazilOeste Paulista Univ, Presidente Prudente, SP, BrazilJose do Rosario Vellano Univ, Alfenas, MG, BrazilFMVZ UNESP, Dept Radiol & Anim Reprod, Botucatu, SP, BrazilFMVZ UNESP, Dept Radiol & Anim Reprod, Botucatu, SP, BrazilFAPESP: 2011/08798-6FAPESP: 2012/03912-8Cryo LettersUniv ParanaenseOeste Paulista UnivJose do Rosario Vellano UnivUniversidade Estadual Paulista (Unesp)Freitas Guaitolini, Carlos Renato deDias Maziero, Rosiara RosariaSouza Castilho, Anthony Cesar dePagnano Derussi, Ana AugustaVolpato, Rodrigo [UNESP]Ackermann, Camila Louise [UNESP]Matsubara, Lidia Mitsuko [UNESP]Lopes, Maria Denise [UNESP]2021-06-25T12:34:34Z2021-06-25T12:34:34Z2020-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article330-336Cryoletters. London: Cryo Letters, v. 41, n. 6, p. 330-336, 2020.0143-2044http://hdl.handle.net/11449/209949WOS:000610484500004Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCryolettersinfo:eu-repo/semantics/openAccess2024-09-09T14:05:44Zoai:repositorio.unesp.br:11449/209949Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:05:44Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
GENE EXPRESSION OF FRESH AND FROZEN/THAWED CANINE EMBRYOS |
title |
GENE EXPRESSION OF FRESH AND FROZEN/THAWED CANINE EMBRYOS |
spellingShingle |
GENE EXPRESSION OF FRESH AND FROZEN/THAWED CANINE EMBRYOS Freitas Guaitolini, Carlos Renato de genes embryos slow freezing |
title_short |
GENE EXPRESSION OF FRESH AND FROZEN/THAWED CANINE EMBRYOS |
title_full |
GENE EXPRESSION OF FRESH AND FROZEN/THAWED CANINE EMBRYOS |
title_fullStr |
GENE EXPRESSION OF FRESH AND FROZEN/THAWED CANINE EMBRYOS |
title_full_unstemmed |
GENE EXPRESSION OF FRESH AND FROZEN/THAWED CANINE EMBRYOS |
title_sort |
GENE EXPRESSION OF FRESH AND FROZEN/THAWED CANINE EMBRYOS |
author |
Freitas Guaitolini, Carlos Renato de |
author_facet |
Freitas Guaitolini, Carlos Renato de Dias Maziero, Rosiara Rosaria Souza Castilho, Anthony Cesar de Pagnano Derussi, Ana Augusta Volpato, Rodrigo [UNESP] Ackermann, Camila Louise [UNESP] Matsubara, Lidia Mitsuko [UNESP] Lopes, Maria Denise [UNESP] |
author_role |
author |
author2 |
Dias Maziero, Rosiara Rosaria Souza Castilho, Anthony Cesar de Pagnano Derussi, Ana Augusta Volpato, Rodrigo [UNESP] Ackermann, Camila Louise [UNESP] Matsubara, Lidia Mitsuko [UNESP] Lopes, Maria Denise [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Paranaense Oeste Paulista Univ Jose do Rosario Vellano Univ Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Freitas Guaitolini, Carlos Renato de Dias Maziero, Rosiara Rosaria Souza Castilho, Anthony Cesar de Pagnano Derussi, Ana Augusta Volpato, Rodrigo [UNESP] Ackermann, Camila Louise [UNESP] Matsubara, Lidia Mitsuko [UNESP] Lopes, Maria Denise [UNESP] |
dc.subject.por.fl_str_mv |
genes embryos slow freezing |
topic |
genes embryos slow freezing |
description |
BACKGROUND: Canine embryo cryopreservation and subsequent transfer are relevant in the use of reproductive technologies. OBJECTIVE: The purpose of this study is the identification and quantification of the gene expression BAX and Bcl2, AQP3, Na+/K+ ATPase alpha 1 and beta 1 and LIFr in canine embryos obtained in vivo and after freezing. MATERIALS AND METHODS: For the collection of embryos, the bitches were identified at pro-estrous until the detection of 80-90% superficial cells. After that, they were artificially inseminated with fresh semen. The embryos were collected after ovariohysterectomy. RNA was extracted and amplified, and embryos were randomly distributed into fresh (Fr) and frozen/thawed (Ft) groups. RESULTS: Eighteen blastocysts were collected from three bitches. Genes BAX, AQP3 and LIFr did not differ among the studied groups. CONCLUSION: We suggest, through these results, that the genes BAX, Bcl2, AQP3, Na + / K + ATPase alpha 1 and beta 1 and LIFr were expressed in canine blastocysts collected in vivo and after slow freezing cryopreservation. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11-01 2021-06-25T12:34:34Z 2021-06-25T12:34:34Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Cryoletters. London: Cryo Letters, v. 41, n. 6, p. 330-336, 2020. 0143-2044 http://hdl.handle.net/11449/209949 WOS:000610484500004 |
identifier_str_mv |
Cryoletters. London: Cryo Letters, v. 41, n. 6, p. 330-336, 2020. 0143-2044 WOS:000610484500004 |
url |
http://hdl.handle.net/11449/209949 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cryoletters |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
330-336 |
dc.publisher.none.fl_str_mv |
Cryo Letters |
publisher.none.fl_str_mv |
Cryo Letters |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021340549218304 |