Alterations in opioid inhibition cause widespread nociception but do not affect anxiety-like behavior in oral cancer mice
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.neuroscience.2017.06.038 http://hdl.handle.net/11449/174996 |
Resumo: | Widespread pain and anxiety are commonly reported in cancer patients. We hypothesize that cancer is accompanied by attenuation of endogenous opioid-mediated inhibition, which subsequently causes widespread pain and anxiety. To test this hypothesis we used a mouse model of oral squamous cell carcinoma (SCC) in the tongue. We found that mice with tongue SCC exhibited widespread nociceptive behaviors in addition to behaviors associated with local nociception that we reported previously. Tongue SCC mice exhibited a pattern of reduced opioid receptor expression in the spinal cord; intrathecal administration of respective mu (MOR), delta (DOR), and kappa (KOR) opioid receptor agonists reduced widespread nociception in mice, except for the fail flick assay following administration of the MOR agonist. We infer from these findings that opioid receptors contribute to widespread nociception in oral cancer mice. Despite significant nociception, mice with tongue SCC did not differ from sham mice in anxiety-like behaviors as measured by the open field assay and elevated maze. No significant differences in c-Fos staining were found in anxiety-associated brain regions in cancer relative to control mice. No correlation was found between nociceptive and anxiety-like behaviors. Moreover, opioid receptor agonists did not yield a statistically significant effect on behaviors measured in the open field and elevated maze in cancer mice. Lastly, we used an acute cancer pain model (injection of cancer supernatant into the mouse tongue) to test whether adaptation to chronic pain is responsible for the absence of greater anxiety-like behavior in cancer mice. No changes in anxiety-like behavior were observed in mice with acute cancer pain. |
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Alterations in opioid inhibition cause widespread nociception but do not affect anxiety-like behavior in oral cancer miceallodyniaanxietycancer painhead and necknociceptionwidespread painWidespread pain and anxiety are commonly reported in cancer patients. We hypothesize that cancer is accompanied by attenuation of endogenous opioid-mediated inhibition, which subsequently causes widespread pain and anxiety. To test this hypothesis we used a mouse model of oral squamous cell carcinoma (SCC) in the tongue. We found that mice with tongue SCC exhibited widespread nociceptive behaviors in addition to behaviors associated with local nociception that we reported previously. Tongue SCC mice exhibited a pattern of reduced opioid receptor expression in the spinal cord; intrathecal administration of respective mu (MOR), delta (DOR), and kappa (KOR) opioid receptor agonists reduced widespread nociception in mice, except for the fail flick assay following administration of the MOR agonist. We infer from these findings that opioid receptors contribute to widespread nociception in oral cancer mice. Despite significant nociception, mice with tongue SCC did not differ from sham mice in anxiety-like behaviors as measured by the open field assay and elevated maze. No significant differences in c-Fos staining were found in anxiety-associated brain regions in cancer relative to control mice. No correlation was found between nociceptive and anxiety-like behaviors. Moreover, opioid receptor agonists did not yield a statistically significant effect on behaviors measured in the open field and elevated maze in cancer mice. Lastly, we used an acute cancer pain model (injection of cancer supernatant into the mouse tongue) to test whether adaptation to chronic pain is responsible for the absence of greater anxiety-like behavior in cancer mice. No changes in anxiety-like behavior were observed in mice with acute cancer pain.National Institute of Dental and Craniofacial ResearchNational Institutes of HealthBluestone Center for Clinical Research College of Dentistry New York UniversityDepartment of Oral Maxillofacial Surgery College of Dentistry New York UniversityEpidemiology and Health Promotion College of Dentistry New York UniversityDepartment of Physiological Nursing School of Nursing University of California at San FranciscoOral Oncology Center and Department of Pathology and Clinical Propedeutics Araçatuba Dental School UNESP – Univ. Estadual Paulista AraçatubaOral Oncology Center and Department of Pathology and Clinical Propedeutics Araçatuba Dental School UNESP – Univ. Estadual Paulista AraçatubaNational Institute of Dental and Craniofacial Research: R01 DE019796National Institute of Dental and Craniofacial Research: R21 DE018561New York UniversityUniversity of California at San FranciscoUniversidade Estadual Paulista (Unesp)Ye, YiBernabé, Daniel G. [UNESP]Salvo, ElizabethViet, Chi T.Ono, KentaroDolan, John C.Janal, MalvinAouizerat, Brad E.Miaskowski, ChristineSchmidt, Brian L.2018-12-11T17:13:46Z2018-12-11T17:13:46Z2017-11-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article50-61application/pdfhttp://dx.doi.org/10.1016/j.neuroscience.2017.06.038Neuroscience, v. 363, p. 50-61.1873-75440306-4522http://hdl.handle.net/11449/17499610.1016/j.neuroscience.2017.06.0382-s2.0-850267720692-s2.0-85026772069.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNeuroscience1,602info:eu-repo/semantics/openAccess2024-04-11T20:16:33Zoai:repositorio.unesp.br:11449/174996Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:12:14.411577Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Alterations in opioid inhibition cause widespread nociception but do not affect anxiety-like behavior in oral cancer mice |
title |
Alterations in opioid inhibition cause widespread nociception but do not affect anxiety-like behavior in oral cancer mice |
spellingShingle |
Alterations in opioid inhibition cause widespread nociception but do not affect anxiety-like behavior in oral cancer mice Ye, Yi allodynia anxiety cancer pain head and neck nociception widespread pain |
title_short |
Alterations in opioid inhibition cause widespread nociception but do not affect anxiety-like behavior in oral cancer mice |
title_full |
Alterations in opioid inhibition cause widespread nociception but do not affect anxiety-like behavior in oral cancer mice |
title_fullStr |
Alterations in opioid inhibition cause widespread nociception but do not affect anxiety-like behavior in oral cancer mice |
title_full_unstemmed |
Alterations in opioid inhibition cause widespread nociception but do not affect anxiety-like behavior in oral cancer mice |
title_sort |
Alterations in opioid inhibition cause widespread nociception but do not affect anxiety-like behavior in oral cancer mice |
author |
Ye, Yi |
author_facet |
Ye, Yi Bernabé, Daniel G. [UNESP] Salvo, Elizabeth Viet, Chi T. Ono, Kentaro Dolan, John C. Janal, Malvin Aouizerat, Brad E. Miaskowski, Christine Schmidt, Brian L. |
author_role |
author |
author2 |
Bernabé, Daniel G. [UNESP] Salvo, Elizabeth Viet, Chi T. Ono, Kentaro Dolan, John C. Janal, Malvin Aouizerat, Brad E. Miaskowski, Christine Schmidt, Brian L. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
New York University University of California at San Francisco Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ye, Yi Bernabé, Daniel G. [UNESP] Salvo, Elizabeth Viet, Chi T. Ono, Kentaro Dolan, John C. Janal, Malvin Aouizerat, Brad E. Miaskowski, Christine Schmidt, Brian L. |
dc.subject.por.fl_str_mv |
allodynia anxiety cancer pain head and neck nociception widespread pain |
topic |
allodynia anxiety cancer pain head and neck nociception widespread pain |
description |
Widespread pain and anxiety are commonly reported in cancer patients. We hypothesize that cancer is accompanied by attenuation of endogenous opioid-mediated inhibition, which subsequently causes widespread pain and anxiety. To test this hypothesis we used a mouse model of oral squamous cell carcinoma (SCC) in the tongue. We found that mice with tongue SCC exhibited widespread nociceptive behaviors in addition to behaviors associated with local nociception that we reported previously. Tongue SCC mice exhibited a pattern of reduced opioid receptor expression in the spinal cord; intrathecal administration of respective mu (MOR), delta (DOR), and kappa (KOR) opioid receptor agonists reduced widespread nociception in mice, except for the fail flick assay following administration of the MOR agonist. We infer from these findings that opioid receptors contribute to widespread nociception in oral cancer mice. Despite significant nociception, mice with tongue SCC did not differ from sham mice in anxiety-like behaviors as measured by the open field assay and elevated maze. No significant differences in c-Fos staining were found in anxiety-associated brain regions in cancer relative to control mice. No correlation was found between nociceptive and anxiety-like behaviors. Moreover, opioid receptor agonists did not yield a statistically significant effect on behaviors measured in the open field and elevated maze in cancer mice. Lastly, we used an acute cancer pain model (injection of cancer supernatant into the mouse tongue) to test whether adaptation to chronic pain is responsible for the absence of greater anxiety-like behavior in cancer mice. No changes in anxiety-like behavior were observed in mice with acute cancer pain. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-05 2018-12-11T17:13:46Z 2018-12-11T17:13:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.neuroscience.2017.06.038 Neuroscience, v. 363, p. 50-61. 1873-7544 0306-4522 http://hdl.handle.net/11449/174996 10.1016/j.neuroscience.2017.06.038 2-s2.0-85026772069 2-s2.0-85026772069.pdf |
url |
http://dx.doi.org/10.1016/j.neuroscience.2017.06.038 http://hdl.handle.net/11449/174996 |
identifier_str_mv |
Neuroscience, v. 363, p. 50-61. 1873-7544 0306-4522 10.1016/j.neuroscience.2017.06.038 2-s2.0-85026772069 2-s2.0-85026772069.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Neuroscience 1,602 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
50-61 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128331995938816 |