Ticks and Rickettsia on anteaters from Southeast and Central-West Brazil

Detalhes bibliográficos
Autor(a) principal: Juan Szabo, Matias Pablo
Data de Publicação: 2019
Outros Autores: Pascoal, Jamile Oliveira, Martins, Maria Marlene, Ramos, Vanessa do Nascimento, Osava, Carolina Fonseca, Quagliatto Santos, Andre Luis, Yokosawa, Jonny, Rezende, Lais Miguel, Tolesano-Pascoli, Graziela Virginia, Torga, Khelma, Castro, Marcio Botelho de, Suzin, Adriane, Mar Barbieri, Amalia Regina, Werther, Karin [UNESP], Magnino Silva, Juliana Macedo, Labruna, Marcelo Bahia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.ttbdis.2019.01.008
http://hdl.handle.net/11449/185562
Resumo: The giant anteater (Myrmecophaga tridactyla) and the collared anteater (Tamandua tetradactyla) are widespread in Brazil and found in all Brazilian biomes. These hosts frequently use domestic animal environments such as pastures, where tick and related microorganism interchange may occur between hosts. Reports of tick infestations of these animals are scattered and refer to small samples and/or are geographically restricted. We herein present data on a wide geographic distribution of ticks and their Rickettsia collected from 72 giant and 30 collared anteaters, mostly road killed, over a period of 18 years, from Southeast and Central-West Brazil encompassing four States and 46 Municipalities. Overall nine tick species (Amblyomma auricularium, A. calcaratum, A. nodosum, A. ovale, A. parvum, A. sculptum, A. triste, Rhipicephalus microplus and R. sanguineus sensu lato) were collected from anteaters. Amblyomma sculptum, A. nodosum, and A. calcaratum were the most prevalent corresponding to, respectively, 48.8%, 39.3% and 2.7% of all ticks (n = 1775). However, A. nodosum tick numbers on collared anteaters were significantly higher (P < 0.001) than those on giant anteaters. At the same time, an abundance of A. sculptum adults on giant anteaters was significantly higher (Z = 2.875; P = 0.004) than that of A. nodosum and only eight A. sculptum nymphs were found on collared anteaters. DNA samples from 20 ticks from nine different animals yielded a visible amplicon in PCR targeting gltA. The PCR products targeting spotted-fever Rickettsia gene (ompA) from five adults of A. nodosum were sequenced and were shown to be 100% identical to Rickettsia parkeri strain NOD (MF737635.1). The product of one nymph and one adult of A. sculptum yielded a sequence 99% identical to R. parkeri strain NOD. Further, Rickettsia bellii genes were found in three A. nodosum adults. Ecological, behavioral and anatomical traits of anteaters are discussed to explain reported tick infestations and Rickettsia DNA found.
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spelling Ticks and Rickettsia on anteaters from Southeast and Central-West BrazilTicksAnteaterBrazilRickettsiaThe giant anteater (Myrmecophaga tridactyla) and the collared anteater (Tamandua tetradactyla) are widespread in Brazil and found in all Brazilian biomes. These hosts frequently use domestic animal environments such as pastures, where tick and related microorganism interchange may occur between hosts. Reports of tick infestations of these animals are scattered and refer to small samples and/or are geographically restricted. We herein present data on a wide geographic distribution of ticks and their Rickettsia collected from 72 giant and 30 collared anteaters, mostly road killed, over a period of 18 years, from Southeast and Central-West Brazil encompassing four States and 46 Municipalities. Overall nine tick species (Amblyomma auricularium, A. calcaratum, A. nodosum, A. ovale, A. parvum, A. sculptum, A. triste, Rhipicephalus microplus and R. sanguineus sensu lato) were collected from anteaters. Amblyomma sculptum, A. nodosum, and A. calcaratum were the most prevalent corresponding to, respectively, 48.8%, 39.3% and 2.7% of all ticks (n = 1775). However, A. nodosum tick numbers on collared anteaters were significantly higher (P < 0.001) than those on giant anteaters. At the same time, an abundance of A. sculptum adults on giant anteaters was significantly higher (Z = 2.875; P = 0.004) than that of A. nodosum and only eight A. sculptum nymphs were found on collared anteaters. DNA samples from 20 ticks from nine different animals yielded a visible amplicon in PCR targeting gltA. The PCR products targeting spotted-fever Rickettsia gene (ompA) from five adults of A. nodosum were sequenced and were shown to be 100% identical to Rickettsia parkeri strain NOD (MF737635.1). The product of one nymph and one adult of A. sculptum yielded a sequence 99% identical to R. parkeri strain NOD. Further, Rickettsia bellii genes were found in three A. nodosum adults. Ecological, behavioral and anatomical traits of anteaters are discussed to explain reported tick infestations and Rickettsia DNA found.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)Univ Fed Uberlandia, Fac Med Vet, Lab Ixodol, Av Para 1720,Campus Umuarama Bloco 2T, BR-38400902 Uberlandia, MG, BrazilInst Fed Goiano, Campus Urutai,Rod Geraldo S Nascimento Km 2,5, BR-75790000 Urutai, Go, BrazilUniv Fed Uberlandia, Inst Ciencias Biomed, Lab Virol, Av Para 1720,Campus Umuarama Bloco 2B, BR-38400902 Uberlandia, MG, BrazilUniv Brasilia, Lab Patol Vet, Av L4 Norte,Hosp Vet Campus Univ Darcy Ribeiro, BR-70910970 Brasilia, DF, BrazilUniv Sao Paulo, Fac Med Vet & Zootecn, Dept Med Vet Prevent & Saude Anim, Av Prof Orlando Marques de Paiva 87,Cidade Univ, BR-05508270 Sao Paulo, SP, BrazilUniv Estadual Paulista, Dept Patol, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilInst Estadual Florestas, Praca Tubal Vilela 03, BR-38400186 Uberlandia, MG, BrazilUniv Estadual Paulista, Dept Patol, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilElsevier B.V.Universidade Federal de Uberlândia (UFU)Inst Fed GoianoUniversidade de Brasília (UnB)Universidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Inst Estadual FlorestasJuan Szabo, Matias PabloPascoal, Jamile OliveiraMartins, Maria MarleneRamos, Vanessa do NascimentoOsava, Carolina FonsecaQuagliatto Santos, Andre LuisYokosawa, JonnyRezende, Lais MiguelTolesano-Pascoli, Graziela VirginiaTorga, KhelmaCastro, Marcio Botelho deSuzin, AdrianeMar Barbieri, Amalia ReginaWerther, Karin [UNESP]Magnino Silva, Juliana MacedoLabruna, Marcelo Bahia2019-10-04T12:36:31Z2019-10-04T12:36:31Z2019-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article540-545http://dx.doi.org/10.1016/j.ttbdis.2019.01.008Ticks And Tick-borne Diseases. Munich: Elsevier Gmbh, v. 10, n. 3, p. 540-545, 2019.1877-959Xhttp://hdl.handle.net/11449/18556210.1016/j.ttbdis.2019.01.008WOS:000462352500007Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTicks And Tick-borne Diseasesinfo:eu-repo/semantics/openAccess2024-06-07T13:03:08Zoai:repositorio.unesp.br:11449/185562Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:52:55.592928Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Ticks and Rickettsia on anteaters from Southeast and Central-West Brazil
title Ticks and Rickettsia on anteaters from Southeast and Central-West Brazil
spellingShingle Ticks and Rickettsia on anteaters from Southeast and Central-West Brazil
Juan Szabo, Matias Pablo
Ticks
Anteater
Brazil
Rickettsia
title_short Ticks and Rickettsia on anteaters from Southeast and Central-West Brazil
title_full Ticks and Rickettsia on anteaters from Southeast and Central-West Brazil
title_fullStr Ticks and Rickettsia on anteaters from Southeast and Central-West Brazil
title_full_unstemmed Ticks and Rickettsia on anteaters from Southeast and Central-West Brazil
title_sort Ticks and Rickettsia on anteaters from Southeast and Central-West Brazil
author Juan Szabo, Matias Pablo
author_facet Juan Szabo, Matias Pablo
Pascoal, Jamile Oliveira
Martins, Maria Marlene
Ramos, Vanessa do Nascimento
Osava, Carolina Fonseca
Quagliatto Santos, Andre Luis
Yokosawa, Jonny
Rezende, Lais Miguel
Tolesano-Pascoli, Graziela Virginia
Torga, Khelma
Castro, Marcio Botelho de
Suzin, Adriane
Mar Barbieri, Amalia Regina
Werther, Karin [UNESP]
Magnino Silva, Juliana Macedo
Labruna, Marcelo Bahia
author_role author
author2 Pascoal, Jamile Oliveira
Martins, Maria Marlene
Ramos, Vanessa do Nascimento
Osava, Carolina Fonseca
Quagliatto Santos, Andre Luis
Yokosawa, Jonny
Rezende, Lais Miguel
Tolesano-Pascoli, Graziela Virginia
Torga, Khelma
Castro, Marcio Botelho de
Suzin, Adriane
Mar Barbieri, Amalia Regina
Werther, Karin [UNESP]
Magnino Silva, Juliana Macedo
Labruna, Marcelo Bahia
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Uberlândia (UFU)
Inst Fed Goiano
Universidade de Brasília (UnB)
Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
Inst Estadual Florestas
dc.contributor.author.fl_str_mv Juan Szabo, Matias Pablo
Pascoal, Jamile Oliveira
Martins, Maria Marlene
Ramos, Vanessa do Nascimento
Osava, Carolina Fonseca
Quagliatto Santos, Andre Luis
Yokosawa, Jonny
Rezende, Lais Miguel
Tolesano-Pascoli, Graziela Virginia
Torga, Khelma
Castro, Marcio Botelho de
Suzin, Adriane
Mar Barbieri, Amalia Regina
Werther, Karin [UNESP]
Magnino Silva, Juliana Macedo
Labruna, Marcelo Bahia
dc.subject.por.fl_str_mv Ticks
Anteater
Brazil
Rickettsia
topic Ticks
Anteater
Brazil
Rickettsia
description The giant anteater (Myrmecophaga tridactyla) and the collared anteater (Tamandua tetradactyla) are widespread in Brazil and found in all Brazilian biomes. These hosts frequently use domestic animal environments such as pastures, where tick and related microorganism interchange may occur between hosts. Reports of tick infestations of these animals are scattered and refer to small samples and/or are geographically restricted. We herein present data on a wide geographic distribution of ticks and their Rickettsia collected from 72 giant and 30 collared anteaters, mostly road killed, over a period of 18 years, from Southeast and Central-West Brazil encompassing four States and 46 Municipalities. Overall nine tick species (Amblyomma auricularium, A. calcaratum, A. nodosum, A. ovale, A. parvum, A. sculptum, A. triste, Rhipicephalus microplus and R. sanguineus sensu lato) were collected from anteaters. Amblyomma sculptum, A. nodosum, and A. calcaratum were the most prevalent corresponding to, respectively, 48.8%, 39.3% and 2.7% of all ticks (n = 1775). However, A. nodosum tick numbers on collared anteaters were significantly higher (P < 0.001) than those on giant anteaters. At the same time, an abundance of A. sculptum adults on giant anteaters was significantly higher (Z = 2.875; P = 0.004) than that of A. nodosum and only eight A. sculptum nymphs were found on collared anteaters. DNA samples from 20 ticks from nine different animals yielded a visible amplicon in PCR targeting gltA. The PCR products targeting spotted-fever Rickettsia gene (ompA) from five adults of A. nodosum were sequenced and were shown to be 100% identical to Rickettsia parkeri strain NOD (MF737635.1). The product of one nymph and one adult of A. sculptum yielded a sequence 99% identical to R. parkeri strain NOD. Further, Rickettsia bellii genes were found in three A. nodosum adults. Ecological, behavioral and anatomical traits of anteaters are discussed to explain reported tick infestations and Rickettsia DNA found.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-04T12:36:31Z
2019-10-04T12:36:31Z
2019-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.ttbdis.2019.01.008
Ticks And Tick-borne Diseases. Munich: Elsevier Gmbh, v. 10, n. 3, p. 540-545, 2019.
1877-959X
http://hdl.handle.net/11449/185562
10.1016/j.ttbdis.2019.01.008
WOS:000462352500007
url http://dx.doi.org/10.1016/j.ttbdis.2019.01.008
http://hdl.handle.net/11449/185562
identifier_str_mv Ticks And Tick-borne Diseases. Munich: Elsevier Gmbh, v. 10, n. 3, p. 540-545, 2019.
1877-959X
10.1016/j.ttbdis.2019.01.008
WOS:000462352500007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ticks And Tick-borne Diseases
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 540-545
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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