Avaliação dos efeitos da terapia fotodinâmica com hipericina no tratamento da doença periodontal induzida em ratos

• Autor(a) principal: Valverde, Livia Rodrigues Perussi [UNESP]
• Data de Publicação: 2013
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/108429
• topic: Fotoquimioterapia; Periodontite; Periodontia; Photochemotherapy

Photodynamic therapy (PDT) has been proposed as an auxiliary therapy on the treatment of peridontitis. This technique uses a non-toxic dye (photosensitizer, PS) that reacts with oxygen of the tissues in the presence of light, and is able to eliminate or reduce microorganisms on the sites of the disease. Hypericin (HY) is a very potent photoactive natural pigment. The aim of this study was evaluate the effects of HY-PDT in the treatment of periodontal disease induced in vivo. Thus, 84 rats were submitted to peridontitis induction by placing ligatures around superior 2nd molars. After 7 days, ligatures were removed and animals were randomically distributed in 4 groups and submitted to the following procedures: Group I – Control, disease without treatment; Group II – SRP, scaling and root planning with manual periodontal curettes; Group III – PDT, application of HY (10μg/ml) for 5 minutes in the periodontal pocket followed by 4 minutes of 630 nm irradiation per tooth (35,15J/cm2); Group IV - SRP+PDT, association of the treatments of groups II and III. Seven, 15 and 30 days post-treatment the animals were sacrificed. MicroCT tridimensional evaluation and histometric analysis showed statistical differences between Control and treatments groups at 7 and 15-days post treatment. TRAP and RANKL immunohistochemistry analysis showed similar marking on groups I, III and IV. Therefore, HY-PDT was able to reduce periodontitis progression in vivo in a similar way to mechanical treatment.