Teeth with acute apical abscess vs. teeth with chronic apical periodontitis: a quantitative and qualitative proteomic analysis
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00784-022-04754-w http://hdl.handle.net/11449/247970 |
Resumo: | Objective: To quantitatively and qualitatively analyze the proteomic profile of teeth with acute apical abscesses (AAA) compared with teeth with chronic apical periodontitis (CAP) and to correlate the expression of detected human proteins with their main biological functions. Materials and methods: Samples were obtained from root canals of 9 patients diagnosed with AAA and 9 with CAP. Samples were analyzed by reversed-phase liquid chromatography coupled to mass spectrometry. Label-free quantitative proteomic analysis was performed by Protein Lynx Global Service software. Differences in protein expression were calculated using the t-test (p < 0.05). Results: In total, 246 human proteins were identified from all samples. Proteins exclusively found in the AAA group were mainly associated with the immunoinflammatory response and oxidative stress response. In the quantitative analysis, 17 proteins were upregulated (p < 0.05) in the AAA group, including alpha-1-acid glycoprotein, hemopexin, fibrinogen gamma chain, and immunoglobulin. Additionally, 61 proteins were downregulated (p < 0.05), comprising cathepsin G, moesin, gelsolin, and transketolase. Most of the proteins were from the extracellular matrix, cytoplasm, and nucleus. Conclusions: The common proteins between the groups were mainly associated with the immune response at both expression levels. Upregulated proteins mostly belonged to the acute-phase proteins, while the downregulated proteins were associated with DNA/RNA regulation and repair, and structural function. Clinical relevance: The host response is directly related to the development of apical abscesses. Thus, understanding the behavior of human proteins against the endodontic pathogens involved in this condition might contribute to the study of new approaches related to the treatment of this disease. |
id |
UNSP_204d304f37581aaab0fd35c19b97e90d |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/247970 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Teeth with acute apical abscess vs. teeth with chronic apical periodontitis: a quantitative and qualitative proteomic analysisApical periodontitisEndodonticsHost–pathogen interactionsPeriapical abscessProteomicsObjective: To quantitatively and qualitatively analyze the proteomic profile of teeth with acute apical abscesses (AAA) compared with teeth with chronic apical periodontitis (CAP) and to correlate the expression of detected human proteins with their main biological functions. Materials and methods: Samples were obtained from root canals of 9 patients diagnosed with AAA and 9 with CAP. Samples were analyzed by reversed-phase liquid chromatography coupled to mass spectrometry. Label-free quantitative proteomic analysis was performed by Protein Lynx Global Service software. Differences in protein expression were calculated using the t-test (p < 0.05). Results: In total, 246 human proteins were identified from all samples. Proteins exclusively found in the AAA group were mainly associated with the immunoinflammatory response and oxidative stress response. In the quantitative analysis, 17 proteins were upregulated (p < 0.05) in the AAA group, including alpha-1-acid glycoprotein, hemopexin, fibrinogen gamma chain, and immunoglobulin. Additionally, 61 proteins were downregulated (p < 0.05), comprising cathepsin G, moesin, gelsolin, and transketolase. Most of the proteins were from the extracellular matrix, cytoplasm, and nucleus. Conclusions: The common proteins between the groups were mainly associated with the immune response at both expression levels. Upregulated proteins mostly belonged to the acute-phase proteins, while the downregulated proteins were associated with DNA/RNA regulation and repair, and structural function. Clinical relevance: The host response is directly related to the development of apical abscesses. Thus, understanding the behavior of human proteins against the endodontic pathogens involved in this condition might contribute to the study of new approaches related to the treatment of this disease.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Preventive and Restorative Dentistry School of Dentistry of Araçatuba São Paulo State University, SPDepartment of Biological Sciences Bauru School of Dentistry University of São Paulo, SPDepartment of Genetics and Evolution Federal University of Sao Carlos, SPDepartment of Preventive and Restorative Dentistry School of Dentistry of Araçatuba São Paulo State University, SPCAPES: 001FAPESP: 2018/18741-0FAPESP: 2019/14995-0Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Universidade Federal de São Carlos (UFSCar)Loureiro, Caroline [UNESP]Buzalaf, Marília Afonso RabeloVentura, Talita Mendes OliveiraPelá, Vinícius TaioquiRodrigues, Gladiston William Lobo [UNESP]Andrade, Júlia Guerra [UNESP]Pessan, Juliano Pelim [UNESP]Jacinto, Rogério Castilho [UNESP]2023-07-29T13:30:54Z2023-07-29T13:30:54Z2023-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article591-601http://dx.doi.org/10.1007/s00784-022-04754-wClinical Oral Investigations, v. 27, n. 2, p. 591-601, 2023.1436-37711432-6981http://hdl.handle.net/11449/24797010.1007/s00784-022-04754-w2-s2.0-85142933271Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigationsinfo:eu-repo/semantics/openAccess2023-07-29T13:30:54Zoai:repositorio.unesp.br:11449/247970Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:30:54Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Teeth with acute apical abscess vs. teeth with chronic apical periodontitis: a quantitative and qualitative proteomic analysis |
title |
Teeth with acute apical abscess vs. teeth with chronic apical periodontitis: a quantitative and qualitative proteomic analysis |
spellingShingle |
Teeth with acute apical abscess vs. teeth with chronic apical periodontitis: a quantitative and qualitative proteomic analysis Loureiro, Caroline [UNESP] Apical periodontitis Endodontics Host–pathogen interactions Periapical abscess Proteomics |
title_short |
Teeth with acute apical abscess vs. teeth with chronic apical periodontitis: a quantitative and qualitative proteomic analysis |
title_full |
Teeth with acute apical abscess vs. teeth with chronic apical periodontitis: a quantitative and qualitative proteomic analysis |
title_fullStr |
Teeth with acute apical abscess vs. teeth with chronic apical periodontitis: a quantitative and qualitative proteomic analysis |
title_full_unstemmed |
Teeth with acute apical abscess vs. teeth with chronic apical periodontitis: a quantitative and qualitative proteomic analysis |
title_sort |
Teeth with acute apical abscess vs. teeth with chronic apical periodontitis: a quantitative and qualitative proteomic analysis |
author |
Loureiro, Caroline [UNESP] |
author_facet |
Loureiro, Caroline [UNESP] Buzalaf, Marília Afonso Rabelo Ventura, Talita Mendes Oliveira Pelá, Vinícius Taioqui Rodrigues, Gladiston William Lobo [UNESP] Andrade, Júlia Guerra [UNESP] Pessan, Juliano Pelim [UNESP] Jacinto, Rogério Castilho [UNESP] |
author_role |
author |
author2 |
Buzalaf, Marília Afonso Rabelo Ventura, Talita Mendes Oliveira Pelá, Vinícius Taioqui Rodrigues, Gladiston William Lobo [UNESP] Andrade, Júlia Guerra [UNESP] Pessan, Juliano Pelim [UNESP] Jacinto, Rogério Castilho [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Loureiro, Caroline [UNESP] Buzalaf, Marília Afonso Rabelo Ventura, Talita Mendes Oliveira Pelá, Vinícius Taioqui Rodrigues, Gladiston William Lobo [UNESP] Andrade, Júlia Guerra [UNESP] Pessan, Juliano Pelim [UNESP] Jacinto, Rogério Castilho [UNESP] |
dc.subject.por.fl_str_mv |
Apical periodontitis Endodontics Host–pathogen interactions Periapical abscess Proteomics |
topic |
Apical periodontitis Endodontics Host–pathogen interactions Periapical abscess Proteomics |
description |
Objective: To quantitatively and qualitatively analyze the proteomic profile of teeth with acute apical abscesses (AAA) compared with teeth with chronic apical periodontitis (CAP) and to correlate the expression of detected human proteins with their main biological functions. Materials and methods: Samples were obtained from root canals of 9 patients diagnosed with AAA and 9 with CAP. Samples were analyzed by reversed-phase liquid chromatography coupled to mass spectrometry. Label-free quantitative proteomic analysis was performed by Protein Lynx Global Service software. Differences in protein expression were calculated using the t-test (p < 0.05). Results: In total, 246 human proteins were identified from all samples. Proteins exclusively found in the AAA group were mainly associated with the immunoinflammatory response and oxidative stress response. In the quantitative analysis, 17 proteins were upregulated (p < 0.05) in the AAA group, including alpha-1-acid glycoprotein, hemopexin, fibrinogen gamma chain, and immunoglobulin. Additionally, 61 proteins were downregulated (p < 0.05), comprising cathepsin G, moesin, gelsolin, and transketolase. Most of the proteins were from the extracellular matrix, cytoplasm, and nucleus. Conclusions: The common proteins between the groups were mainly associated with the immune response at both expression levels. Upregulated proteins mostly belonged to the acute-phase proteins, while the downregulated proteins were associated with DNA/RNA regulation and repair, and structural function. Clinical relevance: The host response is directly related to the development of apical abscesses. Thus, understanding the behavior of human proteins against the endodontic pathogens involved in this condition might contribute to the study of new approaches related to the treatment of this disease. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:30:54Z 2023-07-29T13:30:54Z 2023-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00784-022-04754-w Clinical Oral Investigations, v. 27, n. 2, p. 591-601, 2023. 1436-3771 1432-6981 http://hdl.handle.net/11449/247970 10.1007/s00784-022-04754-w 2-s2.0-85142933271 |
url |
http://dx.doi.org/10.1007/s00784-022-04754-w http://hdl.handle.net/11449/247970 |
identifier_str_mv |
Clinical Oral Investigations, v. 27, n. 2, p. 591-601, 2023. 1436-3771 1432-6981 10.1007/s00784-022-04754-w 2-s2.0-85142933271 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Oral Investigations |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
591-601 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965124637753344 |