Fluoride and calcium concentrations in the biofilm fluid after use of fluoridated dentifrices supplemented with polyphosphate salts
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00784-016-1838-8 http://hdl.handle.net/11449/173001 |
Resumo: | Objectives: The present study evaluated fluoride (F) and calcium (Ca) concentrations in the biofilm fluid formed in situ under cariogenic challenge after using F dentifrices supplemented or not with sodium trimetaphosphate (TMP) or calcium glycerophosphate (CaGP). Methods: Volunteers (n = 12) were randomly divided into 5 groups according to the toothpastes used: placebo (without F, CaGP or TMP), 1100 ppm F (1100F) and low-fluoride dentifrice (LFD, 550 ppm F) with no supplementation (550F) or supplemented with 1 % TMP (550F-TMP) or 0.25 % CaGP (550F-CaGP). In each phase, volunteers wore palatal appliances containing 4 bovine enamel blocks. Cariogenic challenge was performed with 30 % sucrose solution, 6 times/day. On the morning of the eigth day, biofilm samples were collected 12 h and 1 h after brushing and cariogenic challenge. F and Ca analyses in the biofilm fluid were performed with the inverted electrode after buffering with TISAB III and using the Arsenazo III method, respectively. Data were submitted to two-way ANOVA (repeated measures) and Student-Newman-Keuls test (p < 0.05). Results: A dose-response relationship was verified between F concentrations in the dentifrices and in the biofilm fluid. Significant differences were observed among placebo, 550F, and 1100F only 1 h after brushing, without statistical differences among 550F, 550F-TMP, and 550F-CaGP. No defined trend was observed among the groups regarding Ca concentrations, with the highest values seen for placebo and 550F-CaGP. Conclusion: The anticaries effect of LFDs supplemented with CaGP or TMP cannot be related to an increased availability of F and Ca in the biofilm fluid. Clinical significance: The better performance of LFDs containing CaGP or TMP shown in previous studies should be attributed to their ability to interact with tooth enamel and with the biofilm, rather to their effect on the biofilm fluid. |
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Fluoride and calcium concentrations in the biofilm fluid after use of fluoridated dentifrices supplemented with polyphosphate saltsBiofilm fluidCalcium glycerophosphateDentifriceFluorideSodium trimetaphosphateObjectives: The present study evaluated fluoride (F) and calcium (Ca) concentrations in the biofilm fluid formed in situ under cariogenic challenge after using F dentifrices supplemented or not with sodium trimetaphosphate (TMP) or calcium glycerophosphate (CaGP). Methods: Volunteers (n = 12) were randomly divided into 5 groups according to the toothpastes used: placebo (without F, CaGP or TMP), 1100 ppm F (1100F) and low-fluoride dentifrice (LFD, 550 ppm F) with no supplementation (550F) or supplemented with 1 % TMP (550F-TMP) or 0.25 % CaGP (550F-CaGP). In each phase, volunteers wore palatal appliances containing 4 bovine enamel blocks. Cariogenic challenge was performed with 30 % sucrose solution, 6 times/day. On the morning of the eigth day, biofilm samples were collected 12 h and 1 h after brushing and cariogenic challenge. F and Ca analyses in the biofilm fluid were performed with the inverted electrode after buffering with TISAB III and using the Arsenazo III method, respectively. Data were submitted to two-way ANOVA (repeated measures) and Student-Newman-Keuls test (p < 0.05). Results: A dose-response relationship was verified between F concentrations in the dentifrices and in the biofilm fluid. Significant differences were observed among placebo, 550F, and 1100F only 1 h after brushing, without statistical differences among 550F, 550F-TMP, and 550F-CaGP. No defined trend was observed among the groups regarding Ca concentrations, with the highest values seen for placebo and 550F-CaGP. Conclusion: The anticaries effect of LFDs supplemented with CaGP or TMP cannot be related to an increased availability of F and Ca in the biofilm fluid. Clinical significance: The better performance of LFDs containing CaGP or TMP shown in previous studies should be attributed to their ability to interact with tooth enamel and with the biofilm, rather to their effect on the biofilm fluid.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Araçatuba Dental School Univ Estadual Paulista (UNESP)Bauru Dental School University of São PauloDepartment of Pediatric Dentistry and Public Health Araçatuba Dental School Univ Estadual Paulista (UNESP), José Bonifácio 1193Araçatuba Dental School Univ Estadual Paulista (UNESP)Department of Pediatric Dentistry and Public Health Araçatuba Dental School Univ Estadual Paulista (UNESP), José Bonifácio 1193CNPq: 479727/2011-2Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Nagata, Mariana Emi [UNESP]Delbem, Alberto Carlos Botazzo [UNESP]Hall, Kevin Bruce [UNESP]Buzalaf, Marília Afonso RabeloPessan, Juliano Pelim [UNESP]2018-12-11T17:03:04Z2018-12-11T17:03:04Z2017-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article831-837application/pdfhttp://dx.doi.org/10.1007/s00784-016-1838-8Clinical Oral Investigations, v. 21, n. 3, p. 831-837, 2017.1436-37711432-6981http://hdl.handle.net/11449/17300110.1007/s00784-016-1838-82-s2.0-849700283992-s2.0-84970028399.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigations0,9860,986info:eu-repo/semantics/openAccess2024-01-14T06:23:21Zoai:repositorio.unesp.br:11449/173001Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:57:35.053121Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Fluoride and calcium concentrations in the biofilm fluid after use of fluoridated dentifrices supplemented with polyphosphate salts |
title |
Fluoride and calcium concentrations in the biofilm fluid after use of fluoridated dentifrices supplemented with polyphosphate salts |
spellingShingle |
Fluoride and calcium concentrations in the biofilm fluid after use of fluoridated dentifrices supplemented with polyphosphate salts Nagata, Mariana Emi [UNESP] Biofilm fluid Calcium glycerophosphate Dentifrice Fluoride Sodium trimetaphosphate |
title_short |
Fluoride and calcium concentrations in the biofilm fluid after use of fluoridated dentifrices supplemented with polyphosphate salts |
title_full |
Fluoride and calcium concentrations in the biofilm fluid after use of fluoridated dentifrices supplemented with polyphosphate salts |
title_fullStr |
Fluoride and calcium concentrations in the biofilm fluid after use of fluoridated dentifrices supplemented with polyphosphate salts |
title_full_unstemmed |
Fluoride and calcium concentrations in the biofilm fluid after use of fluoridated dentifrices supplemented with polyphosphate salts |
title_sort |
Fluoride and calcium concentrations in the biofilm fluid after use of fluoridated dentifrices supplemented with polyphosphate salts |
author |
Nagata, Mariana Emi [UNESP] |
author_facet |
Nagata, Mariana Emi [UNESP] Delbem, Alberto Carlos Botazzo [UNESP] Hall, Kevin Bruce [UNESP] Buzalaf, Marília Afonso Rabelo Pessan, Juliano Pelim [UNESP] |
author_role |
author |
author2 |
Delbem, Alberto Carlos Botazzo [UNESP] Hall, Kevin Bruce [UNESP] Buzalaf, Marília Afonso Rabelo Pessan, Juliano Pelim [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Nagata, Mariana Emi [UNESP] Delbem, Alberto Carlos Botazzo [UNESP] Hall, Kevin Bruce [UNESP] Buzalaf, Marília Afonso Rabelo Pessan, Juliano Pelim [UNESP] |
dc.subject.por.fl_str_mv |
Biofilm fluid Calcium glycerophosphate Dentifrice Fluoride Sodium trimetaphosphate |
topic |
Biofilm fluid Calcium glycerophosphate Dentifrice Fluoride Sodium trimetaphosphate |
description |
Objectives: The present study evaluated fluoride (F) and calcium (Ca) concentrations in the biofilm fluid formed in situ under cariogenic challenge after using F dentifrices supplemented or not with sodium trimetaphosphate (TMP) or calcium glycerophosphate (CaGP). Methods: Volunteers (n = 12) were randomly divided into 5 groups according to the toothpastes used: placebo (without F, CaGP or TMP), 1100 ppm F (1100F) and low-fluoride dentifrice (LFD, 550 ppm F) with no supplementation (550F) or supplemented with 1 % TMP (550F-TMP) or 0.25 % CaGP (550F-CaGP). In each phase, volunteers wore palatal appliances containing 4 bovine enamel blocks. Cariogenic challenge was performed with 30 % sucrose solution, 6 times/day. On the morning of the eigth day, biofilm samples were collected 12 h and 1 h after brushing and cariogenic challenge. F and Ca analyses in the biofilm fluid were performed with the inverted electrode after buffering with TISAB III and using the Arsenazo III method, respectively. Data were submitted to two-way ANOVA (repeated measures) and Student-Newman-Keuls test (p < 0.05). Results: A dose-response relationship was verified between F concentrations in the dentifrices and in the biofilm fluid. Significant differences were observed among placebo, 550F, and 1100F only 1 h after brushing, without statistical differences among 550F, 550F-TMP, and 550F-CaGP. No defined trend was observed among the groups regarding Ca concentrations, with the highest values seen for placebo and 550F-CaGP. Conclusion: The anticaries effect of LFDs supplemented with CaGP or TMP cannot be related to an increased availability of F and Ca in the biofilm fluid. Clinical significance: The better performance of LFDs containing CaGP or TMP shown in previous studies should be attributed to their ability to interact with tooth enamel and with the biofilm, rather to their effect on the biofilm fluid. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-04-01 2018-12-11T17:03:04Z 2018-12-11T17:03:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00784-016-1838-8 Clinical Oral Investigations, v. 21, n. 3, p. 831-837, 2017. 1436-3771 1432-6981 http://hdl.handle.net/11449/173001 10.1007/s00784-016-1838-8 2-s2.0-84970028399 2-s2.0-84970028399.pdf |
url |
http://dx.doi.org/10.1007/s00784-016-1838-8 http://hdl.handle.net/11449/173001 |
identifier_str_mv |
Clinical Oral Investigations, v. 21, n. 3, p. 831-837, 2017. 1436-3771 1432-6981 10.1007/s00784-016-1838-8 2-s2.0-84970028399 2-s2.0-84970028399.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Clinical Oral Investigations 0,986 0,986 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
831-837 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129477983600640 |