Mapping Bone Marrow Cell Response from Senile Female Rats on Ca-P-Doped Titanium Coating
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/ma15031094 http://hdl.handle.net/11449/223355 |
Resumo: | Chemical and topographical surface modifications on dental implants aim to increase the bone surface contact area of the implant and improve osseointegration. This study analyzed the cellular response of undifferentiated mesenchymal stem cells (MSC), derived from senile rats’ femoral bone marrow, when cultured on a bioactive coating (by plasma electrolytic oxidation, PEO, with Ca2+ and P5+ ions), a sandblasting followed by acid-etching (SLA) surface, and a machined surface (MSU). A total of 102 Ti-6Al-4V discs were divided into three groups (n = 34). The surface chemistry was analyzed by energy dispersive spectroscopy (EDS). Cell viability assay, gene expression of osteoblastic markers, and mineralized matrix formation were investigated. The cell growth and viability results were higher for PEO vs. MSU surface (p = 0.001). An increase in cell proliferation from 3 to 7 days (p < 0.05) and from 7 to 10 days (p < 0.05) was noted for PEO and SLA surfaces. Gene expression for OSX, ALP, BSP, and OPN showed a statistical significance (p = 0.001) among groups. In addition, the PEO surface showed a higher mineralized matrix bone formation (p = 0.003). In conclusion, MSC from senile female rats cultured on SLA and PEO surfaces showed similar cellular responses and should be considered for future clinical investigations. |
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Mapping Bone Marrow Cell Response from Senile Female Rats on Ca-P-Doped Titanium CoatingDental implantsOxidationStromal mesenchymal cellsTitanium surfacesChemical and topographical surface modifications on dental implants aim to increase the bone surface contact area of the implant and improve osseointegration. This study analyzed the cellular response of undifferentiated mesenchymal stem cells (MSC), derived from senile rats’ femoral bone marrow, when cultured on a bioactive coating (by plasma electrolytic oxidation, PEO, with Ca2+ and P5+ ions), a sandblasting followed by acid-etching (SLA) surface, and a machined surface (MSU). A total of 102 Ti-6Al-4V discs were divided into three groups (n = 34). The surface chemistry was analyzed by energy dispersive spectroscopy (EDS). Cell viability assay, gene expression of osteoblastic markers, and mineralized matrix formation were investigated. The cell growth and viability results were higher for PEO vs. MSU surface (p = 0.001). An increase in cell proliferation from 3 to 7 days (p < 0.05) and from 7 to 10 days (p < 0.05) was noted for PEO and SLA surfaces. Gene expression for OSX, ALP, BSP, and OPN showed a statistical significance (p = 0.001) among groups. In addition, the PEO surface showed a higher mineralized matrix bone formation (p = 0.003). In conclusion, MSC from senile female rats cultured on SLA and PEO surfaces showed similar cellular responses and should be considered for future clinical investigations.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Diagnosis and Surgery Division of Oral and Maxillofacial Surgery and Implantology School of Dentistry São Paulo State University—UNESPDental Prosthesis Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University—UNESPBone Res Lab School of Dentistry of Ribeirao Preto University of São Paulo (USP)Department of Periodontology and Oral Implantology Dental Research Division University of Guarulhos (UnG)Department of Prosthodontics and Periodontology Piracicaba Dental School University of Campinas (UNICAMP)Department of Restorative Dentistry College of Dentistry University of Illinois at ChicagoDepartment of Diagnosis and Surgery Division of Oral and Maxillofacial Surgery and Implantology School of Dentistry São Paulo State University—UNESPDental Prosthesis Department of Dental Materials and Prosthodontics School of Dentistry São Paulo State University—UNESPFAPESP: 2016/20297-6Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)University of Guarulhos (UnG)Universidade Estadual de Campinas (UNICAMP)University of Illinois at ChicagoFaverani, Leonardo P. [UNESP]Silva, William P. P. [UNESP]de Sousa, Cecília Alves [UNESP]Freitas, GileadeBassi, Ana Paula F. [UNESP]Shibli, Jamil A.Barão, Valentim A. R.Rosa, Adalberto L.Sukotjo, CortinoAssunção, Wirley G. [UNESP]2022-04-28T19:50:10Z2022-04-28T19:50:10Z2022-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ma15031094Materials, v. 15, n. 3, 2022.1996-1944http://hdl.handle.net/11449/22335510.3390/ma150310942-s2.0-85123625342Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMaterialsinfo:eu-repo/semantics/openAccess2022-04-28T19:50:10Zoai:repositorio.unesp.br:11449/223355Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:27:33.776508Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mapping Bone Marrow Cell Response from Senile Female Rats on Ca-P-Doped Titanium Coating |
title |
Mapping Bone Marrow Cell Response from Senile Female Rats on Ca-P-Doped Titanium Coating |
spellingShingle |
Mapping Bone Marrow Cell Response from Senile Female Rats on Ca-P-Doped Titanium Coating Faverani, Leonardo P. [UNESP] Dental implants Oxidation Stromal mesenchymal cells Titanium surfaces |
title_short |
Mapping Bone Marrow Cell Response from Senile Female Rats on Ca-P-Doped Titanium Coating |
title_full |
Mapping Bone Marrow Cell Response from Senile Female Rats on Ca-P-Doped Titanium Coating |
title_fullStr |
Mapping Bone Marrow Cell Response from Senile Female Rats on Ca-P-Doped Titanium Coating |
title_full_unstemmed |
Mapping Bone Marrow Cell Response from Senile Female Rats on Ca-P-Doped Titanium Coating |
title_sort |
Mapping Bone Marrow Cell Response from Senile Female Rats on Ca-P-Doped Titanium Coating |
author |
Faverani, Leonardo P. [UNESP] |
author_facet |
Faverani, Leonardo P. [UNESP] Silva, William P. P. [UNESP] de Sousa, Cecília Alves [UNESP] Freitas, Gileade Bassi, Ana Paula F. [UNESP] Shibli, Jamil A. Barão, Valentim A. R. Rosa, Adalberto L. Sukotjo, Cortino Assunção, Wirley G. [UNESP] |
author_role |
author |
author2 |
Silva, William P. P. [UNESP] de Sousa, Cecília Alves [UNESP] Freitas, Gileade Bassi, Ana Paula F. [UNESP] Shibli, Jamil A. Barão, Valentim A. R. Rosa, Adalberto L. Sukotjo, Cortino Assunção, Wirley G. [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) University of Guarulhos (UnG) Universidade Estadual de Campinas (UNICAMP) University of Illinois at Chicago |
dc.contributor.author.fl_str_mv |
Faverani, Leonardo P. [UNESP] Silva, William P. P. [UNESP] de Sousa, Cecília Alves [UNESP] Freitas, Gileade Bassi, Ana Paula F. [UNESP] Shibli, Jamil A. Barão, Valentim A. R. Rosa, Adalberto L. Sukotjo, Cortino Assunção, Wirley G. [UNESP] |
dc.subject.por.fl_str_mv |
Dental implants Oxidation Stromal mesenchymal cells Titanium surfaces |
topic |
Dental implants Oxidation Stromal mesenchymal cells Titanium surfaces |
description |
Chemical and topographical surface modifications on dental implants aim to increase the bone surface contact area of the implant and improve osseointegration. This study analyzed the cellular response of undifferentiated mesenchymal stem cells (MSC), derived from senile rats’ femoral bone marrow, when cultured on a bioactive coating (by plasma electrolytic oxidation, PEO, with Ca2+ and P5+ ions), a sandblasting followed by acid-etching (SLA) surface, and a machined surface (MSU). A total of 102 Ti-6Al-4V discs were divided into three groups (n = 34). The surface chemistry was analyzed by energy dispersive spectroscopy (EDS). Cell viability assay, gene expression of osteoblastic markers, and mineralized matrix formation were investigated. The cell growth and viability results were higher for PEO vs. MSU surface (p = 0.001). An increase in cell proliferation from 3 to 7 days (p < 0.05) and from 7 to 10 days (p < 0.05) was noted for PEO and SLA surfaces. Gene expression for OSX, ALP, BSP, and OPN showed a statistical significance (p = 0.001) among groups. In addition, the PEO surface showed a higher mineralized matrix bone formation (p = 0.003). In conclusion, MSC from senile female rats cultured on SLA and PEO surfaces showed similar cellular responses and should be considered for future clinical investigations. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-28T19:50:10Z 2022-04-28T19:50:10Z 2022-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ma15031094 Materials, v. 15, n. 3, 2022. 1996-1944 http://hdl.handle.net/11449/223355 10.3390/ma15031094 2-s2.0-85123625342 |
url |
http://dx.doi.org/10.3390/ma15031094 http://hdl.handle.net/11449/223355 |
identifier_str_mv |
Materials, v. 15, n. 3, 2022. 1996-1944 10.3390/ma15031094 2-s2.0-85123625342 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Materials |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129072603070464 |