Detection of SPM and IMP metallo-beta-lactamases in clinical specimens of Pseudomonas aeruginosa from a Brazilian public tertiary hospital

Detalhes bibliográficos
Autor(a) principal: Camargo, Carlos Henrique [UNESP]
Data de Publicação: 2011
Outros Autores: Bruder-Nascimento, Ariane [UNESP], Mondelli, Alessandro Lia [UNESP], Montelli, Augusto Cezar [UNESP], Sadatsune, Terue [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1413-86702011000500011
http://hdl.handle.net/11449/11437
Resumo: Phenotypic and genotypic SPM and IMP metallo-beta-lactamases (MBL) detection and also the determination of minimal inhibitory concentrations (MIC) to imipenem, meropenem and ceftazidime were evaluated in 47 multidrug-resistant Pseudomonas aeruginosa isolates from clinical specimens. Polymerase chain reaction detected 14 positive samples to either bla(SPM) or bla(IMP) genes, while the best phenotypic assay (ceftazidime substrate and mercaptopropionic acid inhibitor) detected 13 of these samples. Imipenem, meropenem and ceftazidime MICs were higher for MBL positive compared to MBL negative isolates. We describe here the SPM and IMP MBL findings in clinical specimens of P. aeruginosa from the University Hospital of Botucatu Medical School, São Paulo, Brazil, that reinforce local studies showing the high spreading of bla(SPM) and bla(IMP) genes among Brazilian clinical isolates.
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spelling Detection of SPM and IMP metallo-beta-lactamases in clinical specimens of Pseudomonas aeruginosa from a Brazilian public tertiary hospitalPseudomonas aeruginosadrug resistancebacterialcarbapenemsmetalloproteinspolymerase chain reactionPhenotypic and genotypic SPM and IMP metallo-beta-lactamases (MBL) detection and also the determination of minimal inhibitory concentrations (MIC) to imipenem, meropenem and ceftazidime were evaluated in 47 multidrug-resistant Pseudomonas aeruginosa isolates from clinical specimens. Polymerase chain reaction detected 14 positive samples to either bla(SPM) or bla(IMP) genes, while the best phenotypic assay (ceftazidime substrate and mercaptopropionic acid inhibitor) detected 13 of these samples. Imipenem, meropenem and ceftazidime MICs were higher for MBL positive compared to MBL negative isolates. We describe here the SPM and IMP MBL findings in clinical specimens of P. aeruginosa from the University Hospital of Botucatu Medical School, São Paulo, Brazil, that reinforce local studies showing the high spreading of bla(SPM) and bla(IMP) genes among Brazilian clinical isolates.Univ Estatual Paulista UNESP, Botucatu, SP, BrazilUNESP, Botucatu, SP, BrazilUniv Estatual Paulista UNESP, Botucatu, SP, BrazilUNESP, Botucatu, SP, BrazilContextoUniversidade Estadual Paulista (Unesp)Camargo, Carlos Henrique [UNESP]Bruder-Nascimento, Ariane [UNESP]Mondelli, Alessandro Lia [UNESP]Montelli, Augusto Cezar [UNESP]Sadatsune, Terue [UNESP]2014-05-20T13:33:24Z2014-05-20T13:33:24Z2011-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article478-481application/pdfhttp://dx.doi.org/10.1590/S1413-86702011000500011Brazilian Journal of Infectious Diseases. Salvador: Contexto, v. 15, n. 5, p. 478-481, 2011.1413-8670http://hdl.handle.net/11449/11437S1413-86702011000500011WOS:000295936600011S1413-86702011000500011-en.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal of Infectious Diseases2.0830,817info:eu-repo/semantics/openAccess2024-08-14T17:36:42Zoai:repositorio.unesp.br:11449/11437Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:36:42Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Detection of SPM and IMP metallo-beta-lactamases in clinical specimens of Pseudomonas aeruginosa from a Brazilian public tertiary hospital
title Detection of SPM and IMP metallo-beta-lactamases in clinical specimens of Pseudomonas aeruginosa from a Brazilian public tertiary hospital
spellingShingle Detection of SPM and IMP metallo-beta-lactamases in clinical specimens of Pseudomonas aeruginosa from a Brazilian public tertiary hospital
Camargo, Carlos Henrique [UNESP]
Pseudomonas aeruginosa
drug resistance
bacterial
carbapenems
metalloproteins
polymerase chain reaction
title_short Detection of SPM and IMP metallo-beta-lactamases in clinical specimens of Pseudomonas aeruginosa from a Brazilian public tertiary hospital
title_full Detection of SPM and IMP metallo-beta-lactamases in clinical specimens of Pseudomonas aeruginosa from a Brazilian public tertiary hospital
title_fullStr Detection of SPM and IMP metallo-beta-lactamases in clinical specimens of Pseudomonas aeruginosa from a Brazilian public tertiary hospital
title_full_unstemmed Detection of SPM and IMP metallo-beta-lactamases in clinical specimens of Pseudomonas aeruginosa from a Brazilian public tertiary hospital
title_sort Detection of SPM and IMP metallo-beta-lactamases in clinical specimens of Pseudomonas aeruginosa from a Brazilian public tertiary hospital
author Camargo, Carlos Henrique [UNESP]
author_facet Camargo, Carlos Henrique [UNESP]
Bruder-Nascimento, Ariane [UNESP]
Mondelli, Alessandro Lia [UNESP]
Montelli, Augusto Cezar [UNESP]
Sadatsune, Terue [UNESP]
author_role author
author2 Bruder-Nascimento, Ariane [UNESP]
Mondelli, Alessandro Lia [UNESP]
Montelli, Augusto Cezar [UNESP]
Sadatsune, Terue [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Camargo, Carlos Henrique [UNESP]
Bruder-Nascimento, Ariane [UNESP]
Mondelli, Alessandro Lia [UNESP]
Montelli, Augusto Cezar [UNESP]
Sadatsune, Terue [UNESP]
dc.subject.por.fl_str_mv Pseudomonas aeruginosa
drug resistance
bacterial
carbapenems
metalloproteins
polymerase chain reaction
topic Pseudomonas aeruginosa
drug resistance
bacterial
carbapenems
metalloproteins
polymerase chain reaction
description Phenotypic and genotypic SPM and IMP metallo-beta-lactamases (MBL) detection and also the determination of minimal inhibitory concentrations (MIC) to imipenem, meropenem and ceftazidime were evaluated in 47 multidrug-resistant Pseudomonas aeruginosa isolates from clinical specimens. Polymerase chain reaction detected 14 positive samples to either bla(SPM) or bla(IMP) genes, while the best phenotypic assay (ceftazidime substrate and mercaptopropionic acid inhibitor) detected 13 of these samples. Imipenem, meropenem and ceftazidime MICs were higher for MBL positive compared to MBL negative isolates. We describe here the SPM and IMP MBL findings in clinical specimens of P. aeruginosa from the University Hospital of Botucatu Medical School, São Paulo, Brazil, that reinforce local studies showing the high spreading of bla(SPM) and bla(IMP) genes among Brazilian clinical isolates.
publishDate 2011
dc.date.none.fl_str_mv 2011-09-01
2014-05-20T13:33:24Z
2014-05-20T13:33:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1413-86702011000500011
Brazilian Journal of Infectious Diseases. Salvador: Contexto, v. 15, n. 5, p. 478-481, 2011.
1413-8670
http://hdl.handle.net/11449/11437
S1413-86702011000500011
WOS:000295936600011
S1413-86702011000500011-en.pdf
url http://dx.doi.org/10.1590/S1413-86702011000500011
http://hdl.handle.net/11449/11437
identifier_str_mv Brazilian Journal of Infectious Diseases. Salvador: Contexto, v. 15, n. 5, p. 478-481, 2011.
1413-8670
S1413-86702011000500011
WOS:000295936600011
S1413-86702011000500011-en.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Brazilian Journal of Infectious Diseases
2.083
0,817
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 478-481
application/pdf
dc.publisher.none.fl_str_mv Contexto
publisher.none.fl_str_mv Contexto
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128199110950912

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