Semen cryopreservation protocols of Mangalarga Marchador stallions

Detalhes bibliográficos
Autor(a) principal: Candeias, Marcela Leite
Data de Publicação: 2012
Outros Autores: Alvarenga, Marco Antonio [UNESP], Carmo, Márcio Teoro do [UNESP], Ferreira, Heder Nunes [UNESP], Maior, Mônica Russo Souto, Torres Filho, Rodolpho de Almeida, Rodrigues, André Luís Rios, Brandão, Felipe Zandonadi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1516-35982012000900004
http://hdl.handle.net/11449/14523
Resumo: The effect of the utilization of three semen protocols (Inra 82®, Merck Gema and Botu-crio®) and two filling techniques (0.25 and 0.50 mL straws) in Mangalarga Marchador stallions were studied in this experiment. Sperm parameters were assessed during processing and post-freezing. No interactions between the protocols and type of filling were observed, so they were assessed separately. Sperm parameters were not altered when the extender was added to the centrifugation; however, there was reduction of motility and strength when freezing extenders were added. The Botu-crio® protocol preserved the parameters of total and progressive sperm motility, smoothed path velocity (µm/s), straight line velocity (µm/s), track velocity (µm/s) and the average and fast spermatozoa percentage better than the others. No difference between the extenders for the percentage of sperm integrity was observed. There was no difference in the responses studied on the filling techniques. The stallions presented better freezing with the use of the Botu-crio® protocol. The best post-freezing viability results were found for semen frozen using the Botu-crio® protocol and there were no differences concerning the sperm quality comparing 0.25 and 0.50 mL straws.
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spelling Semen cryopreservation protocols of Mangalarga Marchador stallionsejaculatedequineextenderstrawThe effect of the utilization of three semen protocols (Inra 82®, Merck Gema and Botu-crio®) and two filling techniques (0.25 and 0.50 mL straws) in Mangalarga Marchador stallions were studied in this experiment. Sperm parameters were assessed during processing and post-freezing. No interactions between the protocols and type of filling were observed, so they were assessed separately. Sperm parameters were not altered when the extender was added to the centrifugation; however, there was reduction of motility and strength when freezing extenders were added. The Botu-crio® protocol preserved the parameters of total and progressive sperm motility, smoothed path velocity (µm/s), straight line velocity (µm/s), track velocity (µm/s) and the average and fast spermatozoa percentage better than the others. No difference between the extenders for the percentage of sperm integrity was observed. There was no difference in the responses studied on the filling techniques. The stallions presented better freezing with the use of the Botu-crio® protocol. The best post-freezing viability results were found for semen frozen using the Botu-crio® protocol and there were no differences concerning the sperm quality comparing 0.25 and 0.50 mL straws.Universidade Federal Fluminense (UFF) Faculdade de VeterináriaUniversidade Estadual Paulista Julio de Mesquita Filho Faculdade de Medicina Veterinária e ZootecniaUniversidade Estadual Paulista Julio de Mesquita Filho Faculdade de Medicina Veterinária e ZootecniaSociedade Brasileira de ZootecniaUniversidade Federal Fluminense (UFF)Universidade Estadual Paulista (Unesp)Candeias, Marcela LeiteAlvarenga, Marco Antonio [UNESP]Carmo, Márcio Teoro do [UNESP]Ferreira, Heder Nunes [UNESP]Maior, Mônica Russo SoutoTorres Filho, Rodolpho de AlmeidaRodrigues, André Luís RiosBrandão, Felipe Zandonadi2013-09-30T20:12:46Z2014-05-20T13:41:51Z2013-09-30T20:12:46Z2014-05-20T13:41:51Z2012-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1989-1995application/pdfhttp://dx.doi.org/10.1590/S1516-35982012000900004Revista Brasileira de Zootecnia. Sociedade Brasileira de Zootecnia, v. 41, n. 9, p. 1989-1995, 2012.1516-3598http://hdl.handle.net/11449/1452310.1590/S1516-35982012000900004S1516-35982012000900004WOS:000311562800004S1516-35982012000900004.pdf0473846154288947SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Zootecnia0,337info:eu-repo/semantics/openAccess2024-09-09T14:06:05Zoai:repositorio.unesp.br:11449/14523Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:06:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Semen cryopreservation protocols of Mangalarga Marchador stallions
title Semen cryopreservation protocols of Mangalarga Marchador stallions
spellingShingle Semen cryopreservation protocols of Mangalarga Marchador stallions
Candeias, Marcela Leite
ejaculated
equine
extender
straw
title_short Semen cryopreservation protocols of Mangalarga Marchador stallions
title_full Semen cryopreservation protocols of Mangalarga Marchador stallions
title_fullStr Semen cryopreservation protocols of Mangalarga Marchador stallions
title_full_unstemmed Semen cryopreservation protocols of Mangalarga Marchador stallions
title_sort Semen cryopreservation protocols of Mangalarga Marchador stallions
author Candeias, Marcela Leite
author_facet Candeias, Marcela Leite
Alvarenga, Marco Antonio [UNESP]
Carmo, Márcio Teoro do [UNESP]
Ferreira, Heder Nunes [UNESP]
Maior, Mônica Russo Souto
Torres Filho, Rodolpho de Almeida
Rodrigues, André Luís Rios
Brandão, Felipe Zandonadi
author_role author
author2 Alvarenga, Marco Antonio [UNESP]
Carmo, Márcio Teoro do [UNESP]
Ferreira, Heder Nunes [UNESP]
Maior, Mônica Russo Souto
Torres Filho, Rodolpho de Almeida
Rodrigues, André Luís Rios
Brandão, Felipe Zandonadi
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal Fluminense (UFF)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Candeias, Marcela Leite
Alvarenga, Marco Antonio [UNESP]
Carmo, Márcio Teoro do [UNESP]
Ferreira, Heder Nunes [UNESP]
Maior, Mônica Russo Souto
Torres Filho, Rodolpho de Almeida
Rodrigues, André Luís Rios
Brandão, Felipe Zandonadi
dc.subject.por.fl_str_mv ejaculated
equine
extender
straw
topic ejaculated
equine
extender
straw
description The effect of the utilization of three semen protocols (Inra 82®, Merck Gema and Botu-crio®) and two filling techniques (0.25 and 0.50 mL straws) in Mangalarga Marchador stallions were studied in this experiment. Sperm parameters were assessed during processing and post-freezing. No interactions between the protocols and type of filling were observed, so they were assessed separately. Sperm parameters were not altered when the extender was added to the centrifugation; however, there was reduction of motility and strength when freezing extenders were added. The Botu-crio® protocol preserved the parameters of total and progressive sperm motility, smoothed path velocity (µm/s), straight line velocity (µm/s), track velocity (µm/s) and the average and fast spermatozoa percentage better than the others. No difference between the extenders for the percentage of sperm integrity was observed. There was no difference in the responses studied on the filling techniques. The stallions presented better freezing with the use of the Botu-crio® protocol. The best post-freezing viability results were found for semen frozen using the Botu-crio® protocol and there were no differences concerning the sperm quality comparing 0.25 and 0.50 mL straws.
publishDate 2012
dc.date.none.fl_str_mv 2012-09-01
2013-09-30T20:12:46Z
2013-09-30T20:12:46Z
2014-05-20T13:41:51Z
2014-05-20T13:41:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1516-35982012000900004
Revista Brasileira de Zootecnia. Sociedade Brasileira de Zootecnia, v. 41, n. 9, p. 1989-1995, 2012.
1516-3598
http://hdl.handle.net/11449/14523
10.1590/S1516-35982012000900004
S1516-35982012000900004
WOS:000311562800004
S1516-35982012000900004.pdf
0473846154288947
url http://dx.doi.org/10.1590/S1516-35982012000900004
http://hdl.handle.net/11449/14523
identifier_str_mv Revista Brasileira de Zootecnia. Sociedade Brasileira de Zootecnia, v. 41, n. 9, p. 1989-1995, 2012.
1516-3598
10.1590/S1516-35982012000900004
S1516-35982012000900004
WOS:000311562800004
S1516-35982012000900004.pdf
0473846154288947
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Brasileira de Zootecnia
0,337
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1989-1995
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Zootecnia
publisher.none.fl_str_mv Sociedade Brasileira de Zootecnia
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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