Semen cryopreservation protocols of Mangalarga Marchador stallions
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1516-35982012000900004 http://hdl.handle.net/11449/14523 |
Resumo: | The effect of the utilization of three semen protocols (Inra 82®, Merck Gema and Botu-crio®) and two filling techniques (0.25 and 0.50 mL straws) in Mangalarga Marchador stallions were studied in this experiment. Sperm parameters were assessed during processing and post-freezing. No interactions between the protocols and type of filling were observed, so they were assessed separately. Sperm parameters were not altered when the extender was added to the centrifugation; however, there was reduction of motility and strength when freezing extenders were added. The Botu-crio® protocol preserved the parameters of total and progressive sperm motility, smoothed path velocity (µm/s), straight line velocity (µm/s), track velocity (µm/s) and the average and fast spermatozoa percentage better than the others. No difference between the extenders for the percentage of sperm integrity was observed. There was no difference in the responses studied on the filling techniques. The stallions presented better freezing with the use of the Botu-crio® protocol. The best post-freezing viability results were found for semen frozen using the Botu-crio® protocol and there were no differences concerning the sperm quality comparing 0.25 and 0.50 mL straws. |
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Semen cryopreservation protocols of Mangalarga Marchador stallionsejaculatedequineextenderstrawThe effect of the utilization of three semen protocols (Inra 82®, Merck Gema and Botu-crio®) and two filling techniques (0.25 and 0.50 mL straws) in Mangalarga Marchador stallions were studied in this experiment. Sperm parameters were assessed during processing and post-freezing. No interactions between the protocols and type of filling were observed, so they were assessed separately. Sperm parameters were not altered when the extender was added to the centrifugation; however, there was reduction of motility and strength when freezing extenders were added. The Botu-crio® protocol preserved the parameters of total and progressive sperm motility, smoothed path velocity (µm/s), straight line velocity (µm/s), track velocity (µm/s) and the average and fast spermatozoa percentage better than the others. No difference between the extenders for the percentage of sperm integrity was observed. There was no difference in the responses studied on the filling techniques. The stallions presented better freezing with the use of the Botu-crio® protocol. The best post-freezing viability results were found for semen frozen using the Botu-crio® protocol and there were no differences concerning the sperm quality comparing 0.25 and 0.50 mL straws.Universidade Federal Fluminense (UFF) Faculdade de VeterináriaUniversidade Estadual Paulista Julio de Mesquita Filho Faculdade de Medicina Veterinária e ZootecniaUniversidade Estadual Paulista Julio de Mesquita Filho Faculdade de Medicina Veterinária e ZootecniaSociedade Brasileira de ZootecniaUniversidade Federal Fluminense (UFF)Universidade Estadual Paulista (Unesp)Candeias, Marcela LeiteAlvarenga, Marco Antonio [UNESP]Carmo, Márcio Teoro do [UNESP]Ferreira, Heder Nunes [UNESP]Maior, Mônica Russo SoutoTorres Filho, Rodolpho de AlmeidaRodrigues, André Luís RiosBrandão, Felipe Zandonadi2013-09-30T20:12:46Z2014-05-20T13:41:51Z2013-09-30T20:12:46Z2014-05-20T13:41:51Z2012-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1989-1995application/pdfhttp://dx.doi.org/10.1590/S1516-35982012000900004Revista Brasileira de Zootecnia. Sociedade Brasileira de Zootecnia, v. 41, n. 9, p. 1989-1995, 2012.1516-3598http://hdl.handle.net/11449/1452310.1590/S1516-35982012000900004S1516-35982012000900004WOS:000311562800004S1516-35982012000900004.pdf0473846154288947SciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Zootecnia0,337info:eu-repo/semantics/openAccess2024-09-09T14:06:05Zoai:repositorio.unesp.br:11449/14523Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:06:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Semen cryopreservation protocols of Mangalarga Marchador stallions |
title |
Semen cryopreservation protocols of Mangalarga Marchador stallions |
spellingShingle |
Semen cryopreservation protocols of Mangalarga Marchador stallions Candeias, Marcela Leite ejaculated equine extender straw |
title_short |
Semen cryopreservation protocols of Mangalarga Marchador stallions |
title_full |
Semen cryopreservation protocols of Mangalarga Marchador stallions |
title_fullStr |
Semen cryopreservation protocols of Mangalarga Marchador stallions |
title_full_unstemmed |
Semen cryopreservation protocols of Mangalarga Marchador stallions |
title_sort |
Semen cryopreservation protocols of Mangalarga Marchador stallions |
author |
Candeias, Marcela Leite |
author_facet |
Candeias, Marcela Leite Alvarenga, Marco Antonio [UNESP] Carmo, Márcio Teoro do [UNESP] Ferreira, Heder Nunes [UNESP] Maior, Mônica Russo Souto Torres Filho, Rodolpho de Almeida Rodrigues, André Luís Rios Brandão, Felipe Zandonadi |
author_role |
author |
author2 |
Alvarenga, Marco Antonio [UNESP] Carmo, Márcio Teoro do [UNESP] Ferreira, Heder Nunes [UNESP] Maior, Mônica Russo Souto Torres Filho, Rodolpho de Almeida Rodrigues, André Luís Rios Brandão, Felipe Zandonadi |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal Fluminense (UFF) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Candeias, Marcela Leite Alvarenga, Marco Antonio [UNESP] Carmo, Márcio Teoro do [UNESP] Ferreira, Heder Nunes [UNESP] Maior, Mônica Russo Souto Torres Filho, Rodolpho de Almeida Rodrigues, André Luís Rios Brandão, Felipe Zandonadi |
dc.subject.por.fl_str_mv |
ejaculated equine extender straw |
topic |
ejaculated equine extender straw |
description |
The effect of the utilization of three semen protocols (Inra 82®, Merck Gema and Botu-crio®) and two filling techniques (0.25 and 0.50 mL straws) in Mangalarga Marchador stallions were studied in this experiment. Sperm parameters were assessed during processing and post-freezing. No interactions between the protocols and type of filling were observed, so they were assessed separately. Sperm parameters were not altered when the extender was added to the centrifugation; however, there was reduction of motility and strength when freezing extenders were added. The Botu-crio® protocol preserved the parameters of total and progressive sperm motility, smoothed path velocity (µm/s), straight line velocity (µm/s), track velocity (µm/s) and the average and fast spermatozoa percentage better than the others. No difference between the extenders for the percentage of sperm integrity was observed. There was no difference in the responses studied on the filling techniques. The stallions presented better freezing with the use of the Botu-crio® protocol. The best post-freezing viability results were found for semen frozen using the Botu-crio® protocol and there were no differences concerning the sperm quality comparing 0.25 and 0.50 mL straws. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-09-01 2013-09-30T20:12:46Z 2013-09-30T20:12:46Z 2014-05-20T13:41:51Z 2014-05-20T13:41:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1516-35982012000900004 Revista Brasileira de Zootecnia. Sociedade Brasileira de Zootecnia, v. 41, n. 9, p. 1989-1995, 2012. 1516-3598 http://hdl.handle.net/11449/14523 10.1590/S1516-35982012000900004 S1516-35982012000900004 WOS:000311562800004 S1516-35982012000900004.pdf 0473846154288947 |
url |
http://dx.doi.org/10.1590/S1516-35982012000900004 http://hdl.handle.net/11449/14523 |
identifier_str_mv |
Revista Brasileira de Zootecnia. Sociedade Brasileira de Zootecnia, v. 41, n. 9, p. 1989-1995, 2012. 1516-3598 10.1590/S1516-35982012000900004 S1516-35982012000900004 WOS:000311562800004 S1516-35982012000900004.pdf 0473846154288947 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Brasileira de Zootecnia 0,337 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1989-1995 application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Zootecnia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Zootecnia |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546610695602176 |