Regulatory effect of PGE2 on microbicidal activity and inflammatory cytokines in canine leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Venturin, Gabriela Lovizutto [UNESP]
Data de Publicação: 2020
Outros Autores: Bragato, Jaqueline Poleto [UNESP], Melo, Larissa Martins [UNESP], Rebech, Gabriela Torres [UNESP], Costa, Sidnei Ferro [UNESP], de Siqueira, Carlos Eduardo [UNESP], Oliveira dos Santos Maciel, Marilene [UNESP], Eugênio, Flávia de Rezende [UNESP], Patto Santos, Paulo Sérgio [UNESP], de Lima, Valéria Marçal Felix [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1111/pim.12713
http://hdl.handle.net/11449/201671
Resumo: Canine leishmaniasis (CanL) is caused by the intracellular parasite Leishmania infantum. Prostaglandin E2 (PGE2) exerts potent regulatory effects on the immune system in experimental model Leishmania infection, but this influence has not yet been studied in CanL. In this study, PGE2 and PGE2 receptor levels and the regulatory effect of PGE2 on arginase activity, NO2, IL-10, IL-17, IFN-γ, TNF-α and parasite load were evaluated in cultures of splenic leucocytes obtained from dogs with CanL in the presence of agonists and inhibitors. Our results showed that splenic leucocytes from dogs with CanL had lower EP2 receptor levels than those of splenic leucocytes from healthy animals. We observed that NO2 levels decreased when the cells were treated with a PGE2 receptor agonist (EP1/EP2/EP3) or COX-2 inhibitor (NS-398) and that TNF-α, IL-17 and IFN-γ cytokine levels decreased when the cells were treated with a PGE2 receptor agonist (EP2) or PGE2 itself. The parasite load in splenic leucocyte cell cultures from dogs with CanL decreased after stimulation of the cells with PGE2. We conclude that Leishmania infection of dogs modulates PGE2 receptors and speculate that the binding of PGE2 to its receptors may activate the microbicidal capacity of cells.
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spelling Regulatory effect of PGE2 on microbicidal activity and inflammatory cytokines in canine leishmaniasisdogsLeishmania spp.prostaglandin E2receptorsCanine leishmaniasis (CanL) is caused by the intracellular parasite Leishmania infantum. Prostaglandin E2 (PGE2) exerts potent regulatory effects on the immune system in experimental model Leishmania infection, but this influence has not yet been studied in CanL. In this study, PGE2 and PGE2 receptor levels and the regulatory effect of PGE2 on arginase activity, NO2, IL-10, IL-17, IFN-γ, TNF-α and parasite load were evaluated in cultures of splenic leucocytes obtained from dogs with CanL in the presence of agonists and inhibitors. Our results showed that splenic leucocytes from dogs with CanL had lower EP2 receptor levels than those of splenic leucocytes from healthy animals. We observed that NO2 levels decreased when the cells were treated with a PGE2 receptor agonist (EP1/EP2/EP3) or COX-2 inhibitor (NS-398) and that TNF-α, IL-17 and IFN-γ cytokine levels decreased when the cells were treated with a PGE2 receptor agonist (EP2) or PGE2 itself. The parasite load in splenic leucocyte cell cultures from dogs with CanL decreased after stimulation of the cells with PGE2. We conclude that Leishmania infection of dogs modulates PGE2 receptors and speculate that the binding of PGE2 to its receptors may activate the microbicidal capacity of cells.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)School of Veterinary Medicine (Faculdade de Medicina Veterinária; FMVA) Sao Paulo State University ‘Júlio de Mesquita Filho’(UNESP)Clinics Department Animal Surgery and Reproduction School of Veterinary Medicine (Faculdade de Medicina Veterinária; FMVA) Sao Paulo State University ‘Júlio de Mesquita Filho’(UNESP)School of Veterinary Medicine (Faculdade de Medicina Veterinária; FMVA) Sao Paulo State University ‘Júlio de Mesquita Filho’(UNESP)Clinics Department Animal Surgery and Reproduction School of Veterinary Medicine (Faculdade de Medicina Veterinária; FMVA) Sao Paulo State University ‘Júlio de Mesquita Filho’(UNESP)FAPESP: 2016/24388-6CNPq: 400063/2016-6Universidade Estadual Paulista (Unesp)Venturin, Gabriela Lovizutto [UNESP]Bragato, Jaqueline Poleto [UNESP]Melo, Larissa Martins [UNESP]Rebech, Gabriela Torres [UNESP]Costa, Sidnei Ferro [UNESP]de Siqueira, Carlos Eduardo [UNESP]Oliveira dos Santos Maciel, Marilene [UNESP]Eugênio, Flávia de Rezende [UNESP]Patto Santos, Paulo Sérgio [UNESP]de Lima, Valéria Marçal Felix [UNESP]2020-12-12T02:38:44Z2020-12-12T02:38:44Z2020-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1111/pim.12713Parasite Immunology, v. 42, n. 6, 2020.1365-30240141-9838http://hdl.handle.net/11449/20167110.1111/pim.127132-s2.0-85082926498Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengParasite Immunologyinfo:eu-repo/semantics/openAccess2024-09-04T18:04:24Zoai:repositorio.unesp.br:11449/201671Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T18:04:24Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Regulatory effect of PGE2 on microbicidal activity and inflammatory cytokines in canine leishmaniasis
title Regulatory effect of PGE2 on microbicidal activity and inflammatory cytokines in canine leishmaniasis
spellingShingle Regulatory effect of PGE2 on microbicidal activity and inflammatory cytokines in canine leishmaniasis
Venturin, Gabriela Lovizutto [UNESP]
dogs
Leishmania spp.
prostaglandin E2
receptors
title_short Regulatory effect of PGE2 on microbicidal activity and inflammatory cytokines in canine leishmaniasis
title_full Regulatory effect of PGE2 on microbicidal activity and inflammatory cytokines in canine leishmaniasis
title_fullStr Regulatory effect of PGE2 on microbicidal activity and inflammatory cytokines in canine leishmaniasis
title_full_unstemmed Regulatory effect of PGE2 on microbicidal activity and inflammatory cytokines in canine leishmaniasis
title_sort Regulatory effect of PGE2 on microbicidal activity and inflammatory cytokines in canine leishmaniasis
author Venturin, Gabriela Lovizutto [UNESP]
author_facet Venturin, Gabriela Lovizutto [UNESP]
Bragato, Jaqueline Poleto [UNESP]
Melo, Larissa Martins [UNESP]
Rebech, Gabriela Torres [UNESP]
Costa, Sidnei Ferro [UNESP]
de Siqueira, Carlos Eduardo [UNESP]
Oliveira dos Santos Maciel, Marilene [UNESP]
Eugênio, Flávia de Rezende [UNESP]
Patto Santos, Paulo Sérgio [UNESP]
de Lima, Valéria Marçal Felix [UNESP]
author_role author
author2 Bragato, Jaqueline Poleto [UNESP]
Melo, Larissa Martins [UNESP]
Rebech, Gabriela Torres [UNESP]
Costa, Sidnei Ferro [UNESP]
de Siqueira, Carlos Eduardo [UNESP]
Oliveira dos Santos Maciel, Marilene [UNESP]
Eugênio, Flávia de Rezende [UNESP]
Patto Santos, Paulo Sérgio [UNESP]
de Lima, Valéria Marçal Felix [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Venturin, Gabriela Lovizutto [UNESP]
Bragato, Jaqueline Poleto [UNESP]
Melo, Larissa Martins [UNESP]
Rebech, Gabriela Torres [UNESP]
Costa, Sidnei Ferro [UNESP]
de Siqueira, Carlos Eduardo [UNESP]
Oliveira dos Santos Maciel, Marilene [UNESP]
Eugênio, Flávia de Rezende [UNESP]
Patto Santos, Paulo Sérgio [UNESP]
de Lima, Valéria Marçal Felix [UNESP]
dc.subject.por.fl_str_mv dogs
Leishmania spp.
prostaglandin E2
receptors
topic dogs
Leishmania spp.
prostaglandin E2
receptors
description Canine leishmaniasis (CanL) is caused by the intracellular parasite Leishmania infantum. Prostaglandin E2 (PGE2) exerts potent regulatory effects on the immune system in experimental model Leishmania infection, but this influence has not yet been studied in CanL. In this study, PGE2 and PGE2 receptor levels and the regulatory effect of PGE2 on arginase activity, NO2, IL-10, IL-17, IFN-γ, TNF-α and parasite load were evaluated in cultures of splenic leucocytes obtained from dogs with CanL in the presence of agonists and inhibitors. Our results showed that splenic leucocytes from dogs with CanL had lower EP2 receptor levels than those of splenic leucocytes from healthy animals. We observed that NO2 levels decreased when the cells were treated with a PGE2 receptor agonist (EP1/EP2/EP3) or COX-2 inhibitor (NS-398) and that TNF-α, IL-17 and IFN-γ cytokine levels decreased when the cells were treated with a PGE2 receptor agonist (EP2) or PGE2 itself. The parasite load in splenic leucocyte cell cultures from dogs with CanL decreased after stimulation of the cells with PGE2. We conclude that Leishmania infection of dogs modulates PGE2 receptors and speculate that the binding of PGE2 to its receptors may activate the microbicidal capacity of cells.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:38:44Z
2020-12-12T02:38:44Z
2020-06-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1111/pim.12713
Parasite Immunology, v. 42, n. 6, 2020.
1365-3024
0141-9838
http://hdl.handle.net/11449/201671
10.1111/pim.12713
2-s2.0-85082926498
url http://dx.doi.org/10.1111/pim.12713
http://hdl.handle.net/11449/201671
identifier_str_mv Parasite Immunology, v. 42, n. 6, 2020.
1365-3024
0141-9838
10.1111/pim.12713
2-s2.0-85082926498
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Parasite Immunology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1810021423887941632