Regulatory effect of PGE2 on microbicidal activity and inflammatory cytokines in canine leishmaniasis
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/pim.12713 http://hdl.handle.net/11449/201671 |
Resumo: | Canine leishmaniasis (CanL) is caused by the intracellular parasite Leishmania infantum. Prostaglandin E2 (PGE2) exerts potent regulatory effects on the immune system in experimental model Leishmania infection, but this influence has not yet been studied in CanL. In this study, PGE2 and PGE2 receptor levels and the regulatory effect of PGE2 on arginase activity, NO2, IL-10, IL-17, IFN-γ, TNF-α and parasite load were evaluated in cultures of splenic leucocytes obtained from dogs with CanL in the presence of agonists and inhibitors. Our results showed that splenic leucocytes from dogs with CanL had lower EP2 receptor levels than those of splenic leucocytes from healthy animals. We observed that NO2 levels decreased when the cells were treated with a PGE2 receptor agonist (EP1/EP2/EP3) or COX-2 inhibitor (NS-398) and that TNF-α, IL-17 and IFN-γ cytokine levels decreased when the cells were treated with a PGE2 receptor agonist (EP2) or PGE2 itself. The parasite load in splenic leucocyte cell cultures from dogs with CanL decreased after stimulation of the cells with PGE2. We conclude that Leishmania infection of dogs modulates PGE2 receptors and speculate that the binding of PGE2 to its receptors may activate the microbicidal capacity of cells. |
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Regulatory effect of PGE2 on microbicidal activity and inflammatory cytokines in canine leishmaniasisdogsLeishmania spp.prostaglandin E2receptorsCanine leishmaniasis (CanL) is caused by the intracellular parasite Leishmania infantum. Prostaglandin E2 (PGE2) exerts potent regulatory effects on the immune system in experimental model Leishmania infection, but this influence has not yet been studied in CanL. In this study, PGE2 and PGE2 receptor levels and the regulatory effect of PGE2 on arginase activity, NO2, IL-10, IL-17, IFN-γ, TNF-α and parasite load were evaluated in cultures of splenic leucocytes obtained from dogs with CanL in the presence of agonists and inhibitors. Our results showed that splenic leucocytes from dogs with CanL had lower EP2 receptor levels than those of splenic leucocytes from healthy animals. We observed that NO2 levels decreased when the cells were treated with a PGE2 receptor agonist (EP1/EP2/EP3) or COX-2 inhibitor (NS-398) and that TNF-α, IL-17 and IFN-γ cytokine levels decreased when the cells were treated with a PGE2 receptor agonist (EP2) or PGE2 itself. The parasite load in splenic leucocyte cell cultures from dogs with CanL decreased after stimulation of the cells with PGE2. We conclude that Leishmania infection of dogs modulates PGE2 receptors and speculate that the binding of PGE2 to its receptors may activate the microbicidal capacity of cells.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)School of Veterinary Medicine (Faculdade de Medicina Veterinária; FMVA) Sao Paulo State University ‘Júlio de Mesquita Filho’(UNESP)Clinics Department Animal Surgery and Reproduction School of Veterinary Medicine (Faculdade de Medicina Veterinária; FMVA) Sao Paulo State University ‘Júlio de Mesquita Filho’(UNESP)School of Veterinary Medicine (Faculdade de Medicina Veterinária; FMVA) Sao Paulo State University ‘Júlio de Mesquita Filho’(UNESP)Clinics Department Animal Surgery and Reproduction School of Veterinary Medicine (Faculdade de Medicina Veterinária; FMVA) Sao Paulo State University ‘Júlio de Mesquita Filho’(UNESP)FAPESP: 2016/24388-6CNPq: 400063/2016-6Universidade Estadual Paulista (Unesp)Venturin, Gabriela Lovizutto [UNESP]Bragato, Jaqueline Poleto [UNESP]Melo, Larissa Martins [UNESP]Rebech, Gabriela Torres [UNESP]Costa, Sidnei Ferro [UNESP]de Siqueira, Carlos Eduardo [UNESP]Oliveira dos Santos Maciel, Marilene [UNESP]Eugênio, Flávia de Rezende [UNESP]Patto Santos, Paulo Sérgio [UNESP]de Lima, Valéria Marçal Felix [UNESP]2020-12-12T02:38:44Z2020-12-12T02:38:44Z2020-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1111/pim.12713Parasite Immunology, v. 42, n. 6, 2020.1365-30240141-9838http://hdl.handle.net/11449/20167110.1111/pim.127132-s2.0-85082926498Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengParasite Immunologyinfo:eu-repo/semantics/openAccess2024-09-04T18:04:24Zoai:repositorio.unesp.br:11449/201671Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T18:04:24Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Regulatory effect of PGE2 on microbicidal activity and inflammatory cytokines in canine leishmaniasis |
title |
Regulatory effect of PGE2 on microbicidal activity and inflammatory cytokines in canine leishmaniasis |
spellingShingle |
Regulatory effect of PGE2 on microbicidal activity and inflammatory cytokines in canine leishmaniasis Venturin, Gabriela Lovizutto [UNESP] dogs Leishmania spp. prostaglandin E2 receptors |
title_short |
Regulatory effect of PGE2 on microbicidal activity and inflammatory cytokines in canine leishmaniasis |
title_full |
Regulatory effect of PGE2 on microbicidal activity and inflammatory cytokines in canine leishmaniasis |
title_fullStr |
Regulatory effect of PGE2 on microbicidal activity and inflammatory cytokines in canine leishmaniasis |
title_full_unstemmed |
Regulatory effect of PGE2 on microbicidal activity and inflammatory cytokines in canine leishmaniasis |
title_sort |
Regulatory effect of PGE2 on microbicidal activity and inflammatory cytokines in canine leishmaniasis |
author |
Venturin, Gabriela Lovizutto [UNESP] |
author_facet |
Venturin, Gabriela Lovizutto [UNESP] Bragato, Jaqueline Poleto [UNESP] Melo, Larissa Martins [UNESP] Rebech, Gabriela Torres [UNESP] Costa, Sidnei Ferro [UNESP] de Siqueira, Carlos Eduardo [UNESP] Oliveira dos Santos Maciel, Marilene [UNESP] Eugênio, Flávia de Rezende [UNESP] Patto Santos, Paulo Sérgio [UNESP] de Lima, Valéria Marçal Felix [UNESP] |
author_role |
author |
author2 |
Bragato, Jaqueline Poleto [UNESP] Melo, Larissa Martins [UNESP] Rebech, Gabriela Torres [UNESP] Costa, Sidnei Ferro [UNESP] de Siqueira, Carlos Eduardo [UNESP] Oliveira dos Santos Maciel, Marilene [UNESP] Eugênio, Flávia de Rezende [UNESP] Patto Santos, Paulo Sérgio [UNESP] de Lima, Valéria Marçal Felix [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Venturin, Gabriela Lovizutto [UNESP] Bragato, Jaqueline Poleto [UNESP] Melo, Larissa Martins [UNESP] Rebech, Gabriela Torres [UNESP] Costa, Sidnei Ferro [UNESP] de Siqueira, Carlos Eduardo [UNESP] Oliveira dos Santos Maciel, Marilene [UNESP] Eugênio, Flávia de Rezende [UNESP] Patto Santos, Paulo Sérgio [UNESP] de Lima, Valéria Marçal Felix [UNESP] |
dc.subject.por.fl_str_mv |
dogs Leishmania spp. prostaglandin E2 receptors |
topic |
dogs Leishmania spp. prostaglandin E2 receptors |
description |
Canine leishmaniasis (CanL) is caused by the intracellular parasite Leishmania infantum. Prostaglandin E2 (PGE2) exerts potent regulatory effects on the immune system in experimental model Leishmania infection, but this influence has not yet been studied in CanL. In this study, PGE2 and PGE2 receptor levels and the regulatory effect of PGE2 on arginase activity, NO2, IL-10, IL-17, IFN-γ, TNF-α and parasite load were evaluated in cultures of splenic leucocytes obtained from dogs with CanL in the presence of agonists and inhibitors. Our results showed that splenic leucocytes from dogs with CanL had lower EP2 receptor levels than those of splenic leucocytes from healthy animals. We observed that NO2 levels decreased when the cells were treated with a PGE2 receptor agonist (EP1/EP2/EP3) or COX-2 inhibitor (NS-398) and that TNF-α, IL-17 and IFN-γ cytokine levels decreased when the cells were treated with a PGE2 receptor agonist (EP2) or PGE2 itself. The parasite load in splenic leucocyte cell cultures from dogs with CanL decreased after stimulation of the cells with PGE2. We conclude that Leishmania infection of dogs modulates PGE2 receptors and speculate that the binding of PGE2 to its receptors may activate the microbicidal capacity of cells. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:38:44Z 2020-12-12T02:38:44Z 2020-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/pim.12713 Parasite Immunology, v. 42, n. 6, 2020. 1365-3024 0141-9838 http://hdl.handle.net/11449/201671 10.1111/pim.12713 2-s2.0-85082926498 |
url |
http://dx.doi.org/10.1111/pim.12713 http://hdl.handle.net/11449/201671 |
identifier_str_mv |
Parasite Immunology, v. 42, n. 6, 2020. 1365-3024 0141-9838 10.1111/pim.12713 2-s2.0-85082926498 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Parasite Immunology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021423887941632 |