Improvement Production of Hyaluronic Acid by Streptococcus zooepidemicus in Sugarcane Molasses
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNESP |
| Texto Completo: | http://dx.doi.org/10.1007/s12010-016-2326-y http://hdl.handle.net/11449/173864 |
Resumo: | Microbial hyaluronic acid (HA) production has been preferred rather than extraction from animal tissue for medical and cosmetic applications. In this context, to obtain an economically competitive HA production by Streptococcus zooepidemicus, culture conditions were studied to improve the polymer production in sugarcane molasses. The highest HA production by S. zooepidemicus ATCC 39920 achieved was 2.825 g. L−1 in a 4.5 L bioreactor with controlled pH (8.0) and medium containing molasses (85.35 g.L−1 total sugar) pretreated with activated charcoal and yeast extract (50 g.L−1). The HA produced exhibited a high molecular weight of 1.35 × 103 kDa and the DPPH radical scavenging activity of the polymer at 1 g.L−1 was 41 %. The FTIR and UV-Vis spectra showed no substantial differences in the spectral pattern between produced and standard HA. This study is a promising strategy for sugarcane molasses application by producing high value-added products such as hyaluronic acid. |
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Improvement Production of Hyaluronic Acid by Streptococcus zooepidemicus in Sugarcane MolassesBioreactorFermentationHyaluronic acidStreptococcusSugarcane molassesMicrobial hyaluronic acid (HA) production has been preferred rather than extraction from animal tissue for medical and cosmetic applications. In this context, to obtain an economically competitive HA production by Streptococcus zooepidemicus, culture conditions were studied to improve the polymer production in sugarcane molasses. The highest HA production by S. zooepidemicus ATCC 39920 achieved was 2.825 g. L−1 in a 4.5 L bioreactor with controlled pH (8.0) and medium containing molasses (85.35 g.L−1 total sugar) pretreated with activated charcoal and yeast extract (50 g.L−1). The HA produced exhibited a high molecular weight of 1.35 × 103 kDa and the DPPH radical scavenging activity of the polymer at 1 g.L−1 was 41 %. The FTIR and UV-Vis spectra showed no substantial differences in the spectral pattern between produced and standard HA. This study is a promising strategy for sugarcane molasses application by producing high value-added products such as hyaluronic acid.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Departamento de Bioquímica e Biotecnologia Universidade Estadual de Londrina – UEL, Rodovia Celso Garcia Cid - Pr 445 Km 380, Campus Universitário, Caixa Postal 10.011Departamento de Química e Bioquímica Faculdade de Ciências e Tecnologia Universidade Estadual Paulista –UNESP, Rua Roberto Simonsen, n°. 305Departamento de Química e Bioquímica Faculdade de Ciências e Tecnologia Universidade Estadual Paulista –UNESP, Rua Roberto Simonsen, n°. 305Universidade Estadual de Londrina (UEL)Universidade Estadual Paulista (Unesp)Pan, Nicole CaldasPereira, Hanny Cristina Bragada Silva, Mariade.Lourdes.Corradi. [UNESP]Vasconcelos, Ana Flora Dalberto [UNESP]Celligoi, Maria Antonia Pedrine Colabone2018-12-11T17:08:07Z2018-12-11T17:08:07Z2017-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article276-293application/pdfhttp://dx.doi.org/10.1007/s12010-016-2326-yApplied Biochemistry and Biotechnology, v. 182, n. 1, p. 276-293, 2017.1559-02910273-2289http://hdl.handle.net/11449/17386410.1007/s12010-016-2326-y2-s2.0-850008147182-s2.0-85000814718.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengApplied Biochemistry and Biotechnology0,571info:eu-repo/semantics/openAccess2023-10-30T06:11:13Zoai:repositorio.unesp.br:11449/173864Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-30T06:11:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
| dc.title.none.fl_str_mv |
Improvement Production of Hyaluronic Acid by Streptococcus zooepidemicus in Sugarcane Molasses |
| title |
Improvement Production of Hyaluronic Acid by Streptococcus zooepidemicus in Sugarcane Molasses |
| spellingShingle |
Improvement Production of Hyaluronic Acid by Streptococcus zooepidemicus in Sugarcane Molasses Pan, Nicole Caldas Bioreactor Fermentation Hyaluronic acid Streptococcus Sugarcane molasses |
| title_short |
Improvement Production of Hyaluronic Acid by Streptococcus zooepidemicus in Sugarcane Molasses |
| title_full |
Improvement Production of Hyaluronic Acid by Streptococcus zooepidemicus in Sugarcane Molasses |
| title_fullStr |
Improvement Production of Hyaluronic Acid by Streptococcus zooepidemicus in Sugarcane Molasses |
| title_full_unstemmed |
Improvement Production of Hyaluronic Acid by Streptococcus zooepidemicus in Sugarcane Molasses |
| title_sort |
Improvement Production of Hyaluronic Acid by Streptococcus zooepidemicus in Sugarcane Molasses |
| author |
Pan, Nicole Caldas |
| author_facet |
Pan, Nicole Caldas Pereira, Hanny Cristina Braga da Silva, Mariade.Lourdes.Corradi. [UNESP] Vasconcelos, Ana Flora Dalberto [UNESP] Celligoi, Maria Antonia Pedrine Colabone |
| author_role |
author |
| author2 |
Pereira, Hanny Cristina Braga da Silva, Mariade.Lourdes.Corradi. [UNESP] Vasconcelos, Ana Flora Dalberto [UNESP] Celligoi, Maria Antonia Pedrine Colabone |
| author2_role |
author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Estadual de Londrina (UEL) Universidade Estadual Paulista (Unesp) |
| dc.contributor.author.fl_str_mv |
Pan, Nicole Caldas Pereira, Hanny Cristina Braga da Silva, Mariade.Lourdes.Corradi. [UNESP] Vasconcelos, Ana Flora Dalberto [UNESP] Celligoi, Maria Antonia Pedrine Colabone |
| dc.subject.por.fl_str_mv |
Bioreactor Fermentation Hyaluronic acid Streptococcus Sugarcane molasses |
| topic |
Bioreactor Fermentation Hyaluronic acid Streptococcus Sugarcane molasses |
| description |
Microbial hyaluronic acid (HA) production has been preferred rather than extraction from animal tissue for medical and cosmetic applications. In this context, to obtain an economically competitive HA production by Streptococcus zooepidemicus, culture conditions were studied to improve the polymer production in sugarcane molasses. The highest HA production by S. zooepidemicus ATCC 39920 achieved was 2.825 g. L−1 in a 4.5 L bioreactor with controlled pH (8.0) and medium containing molasses (85.35 g.L−1 total sugar) pretreated with activated charcoal and yeast extract (50 g.L−1). The HA produced exhibited a high molecular weight of 1.35 × 103 kDa and the DPPH radical scavenging activity of the polymer at 1 g.L−1 was 41 %. The FTIR and UV-Vis spectra showed no substantial differences in the spectral pattern between produced and standard HA. This study is a promising strategy for sugarcane molasses application by producing high value-added products such as hyaluronic acid. |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017-05-01 2018-12-11T17:08:07Z 2018-12-11T17:08:07Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s12010-016-2326-y Applied Biochemistry and Biotechnology, v. 182, n. 1, p. 276-293, 2017. 1559-0291 0273-2289 http://hdl.handle.net/11449/173864 10.1007/s12010-016-2326-y 2-s2.0-85000814718 2-s2.0-85000814718.pdf |
| url |
http://dx.doi.org/10.1007/s12010-016-2326-y http://hdl.handle.net/11449/173864 |
| identifier_str_mv |
Applied Biochemistry and Biotechnology, v. 182, n. 1, p. 276-293, 2017. 1559-0291 0273-2289 10.1007/s12010-016-2326-y 2-s2.0-85000814718 2-s2.0-85000814718.pdf |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Applied Biochemistry and Biotechnology 0,571 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
276-293 application/pdf |
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Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
| instname_str |
Universidade Estadual Paulista (UNESP) |
| instacron_str |
UNESP |
| institution |
UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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1799964759036002304 |