First record of a new microsporidium pathogenic to Gonipterus platensis in Brazil
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-021-90041-9 http://hdl.handle.net/11449/207802 |
Resumo: | Microsporidia are naturally occurring fungal-related parasites that can infect nearly all animal hosts, but their biocontrol potential of insect pests is routinely overlooked in agriculture and forestry. This research brings the first report describing the natural occurrence of a microsporidium causing disease in field-collected populations of the invasive eucalyptus snout beetle, Gonipterus platensis (Coleoptera: Curculionidae), a major destructive pest of eucalyptus plantations in Brazil. Adult beetles were collected during field surveys in commercial eucalyptus plantations in southern Brazil to be examined and dissected with typical symptoms to verify presence of microsporidian spores in haemolymph. From 14 plantations in different sites, the natural infection occurrence in these populations ranged from 0 to 65%, while a lab colony exhibited an infection incidence of 70%. Spore density in haemolymph of symptomatic insects averaged 2.1 (± 0.4) × 107 spores/beetle. Symptoms in infected adults were identified by an abnormal abdomen with malformation of the second pair of wings, impairing their flight activity. Electron transmission microscopy of the pathogen showed morphological features similar to species belonging to the genus Nosema or Vairimorpha. Phylogenetic analysis of the full-length small subunit ribosomal RNA gene suggests this pathogen’s placement in the genus Vairimorpha, but with a sequence identity of ~ 94% with the nearest neighbours. The low level of sequence identity suggests this pathogen may represent a novel taxon in the genus and further requires whole genome sequencing for definitive taxonomic resolution. These findings provide insights on the natural occurrence of this novel pathogen of this invasive pest in Eucalyptus plantations in Brazil. Further studies are needed to determine potential of this microsporidium in the design of conservative or augmentative biological control programs for this invasive pest. |
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First record of a new microsporidium pathogenic to Gonipterus platensis in BrazilMicrosporidia are naturally occurring fungal-related parasites that can infect nearly all animal hosts, but their biocontrol potential of insect pests is routinely overlooked in agriculture and forestry. This research brings the first report describing the natural occurrence of a microsporidium causing disease in field-collected populations of the invasive eucalyptus snout beetle, Gonipterus platensis (Coleoptera: Curculionidae), a major destructive pest of eucalyptus plantations in Brazil. Adult beetles were collected during field surveys in commercial eucalyptus plantations in southern Brazil to be examined and dissected with typical symptoms to verify presence of microsporidian spores in haemolymph. From 14 plantations in different sites, the natural infection occurrence in these populations ranged from 0 to 65%, while a lab colony exhibited an infection incidence of 70%. Spore density in haemolymph of symptomatic insects averaged 2.1 (± 0.4) × 107 spores/beetle. Symptoms in infected adults were identified by an abnormal abdomen with malformation of the second pair of wings, impairing their flight activity. Electron transmission microscopy of the pathogen showed morphological features similar to species belonging to the genus Nosema or Vairimorpha. Phylogenetic analysis of the full-length small subunit ribosomal RNA gene suggests this pathogen’s placement in the genus Vairimorpha, but with a sequence identity of ~ 94% with the nearest neighbours. The low level of sequence identity suggests this pathogen may represent a novel taxon in the genus and further requires whole genome sequencing for definitive taxonomic resolution. These findings provide insights on the natural occurrence of this novel pathogen of this invasive pest in Eucalyptus plantations in Brazil. Further studies are needed to determine potential of this microsporidium in the design of conservative or augmentative biological control programs for this invasive pest.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)School of Agricultural Sciences São Paulo State University (UNESP), Campus of Botucatu, Av. Universitária, 3780, Altos do Paraíso, Fazenda Experimental LageadoLaboratory of Environmental Microbiology Brazilian Agricultural Research Corporation Embrapa Environment, Rodovia SP-340, km 127.5Botucatu Medical School Dept. Internal Medicine Sao Paulo State University (UNESP), Campus of Botucatu, Av. Prof. Mário Rubens Guimarães Montenegro, s/nUSDA Agricultural Research Service National Center for Agricultural Utilization Research Crop Bioprotection Research Unit, 1815, N. University StSchool of Agricultural Sciences São Paulo State University (UNESP), Campus of Botucatu, Av. Universitária, 3780, Altos do Paraíso, Fazenda Experimental LageadoBotucatu Medical School Dept. Internal Medicine Sao Paulo State University (UNESP), Campus of Botucatu, Av. Prof. Mário Rubens Guimarães Montenegro, s/nUniversidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Crop Bioprotection Research UnitJordan, Carolina [UNESP]de Carvalho, Vanessa Rafaela [UNESP]Mascarin, Gabriel Mourados Santos Oliveira, Leiliane Rodrigues [UNESP]Dunlap, Christopher A.Wilcken, Carlos Frederico [UNESP]2021-06-25T11:01:16Z2021-06-25T11:01:16Z2021-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-021-90041-9Scientific Reports, v. 11, n. 1, 2021.2045-2322http://hdl.handle.net/11449/20780210.1038/s41598-021-90041-92-s2.0-85106918623Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2024-08-14T17:22:37Zoai:repositorio.unesp.br:11449/207802Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:22:37Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
First record of a new microsporidium pathogenic to Gonipterus platensis in Brazil |
title |
First record of a new microsporidium pathogenic to Gonipterus platensis in Brazil |
spellingShingle |
First record of a new microsporidium pathogenic to Gonipterus platensis in Brazil Jordan, Carolina [UNESP] |
title_short |
First record of a new microsporidium pathogenic to Gonipterus platensis in Brazil |
title_full |
First record of a new microsporidium pathogenic to Gonipterus platensis in Brazil |
title_fullStr |
First record of a new microsporidium pathogenic to Gonipterus platensis in Brazil |
title_full_unstemmed |
First record of a new microsporidium pathogenic to Gonipterus platensis in Brazil |
title_sort |
First record of a new microsporidium pathogenic to Gonipterus platensis in Brazil |
author |
Jordan, Carolina [UNESP] |
author_facet |
Jordan, Carolina [UNESP] de Carvalho, Vanessa Rafaela [UNESP] Mascarin, Gabriel Moura dos Santos Oliveira, Leiliane Rodrigues [UNESP] Dunlap, Christopher A. Wilcken, Carlos Frederico [UNESP] |
author_role |
author |
author2 |
de Carvalho, Vanessa Rafaela [UNESP] Mascarin, Gabriel Moura dos Santos Oliveira, Leiliane Rodrigues [UNESP] Dunlap, Christopher A. Wilcken, Carlos Frederico [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Crop Bioprotection Research Unit |
dc.contributor.author.fl_str_mv |
Jordan, Carolina [UNESP] de Carvalho, Vanessa Rafaela [UNESP] Mascarin, Gabriel Moura dos Santos Oliveira, Leiliane Rodrigues [UNESP] Dunlap, Christopher A. Wilcken, Carlos Frederico [UNESP] |
description |
Microsporidia are naturally occurring fungal-related parasites that can infect nearly all animal hosts, but their biocontrol potential of insect pests is routinely overlooked in agriculture and forestry. This research brings the first report describing the natural occurrence of a microsporidium causing disease in field-collected populations of the invasive eucalyptus snout beetle, Gonipterus platensis (Coleoptera: Curculionidae), a major destructive pest of eucalyptus plantations in Brazil. Adult beetles were collected during field surveys in commercial eucalyptus plantations in southern Brazil to be examined and dissected with typical symptoms to verify presence of microsporidian spores in haemolymph. From 14 plantations in different sites, the natural infection occurrence in these populations ranged from 0 to 65%, while a lab colony exhibited an infection incidence of 70%. Spore density in haemolymph of symptomatic insects averaged 2.1 (± 0.4) × 107 spores/beetle. Symptoms in infected adults were identified by an abnormal abdomen with malformation of the second pair of wings, impairing their flight activity. Electron transmission microscopy of the pathogen showed morphological features similar to species belonging to the genus Nosema or Vairimorpha. Phylogenetic analysis of the full-length small subunit ribosomal RNA gene suggests this pathogen’s placement in the genus Vairimorpha, but with a sequence identity of ~ 94% with the nearest neighbours. The low level of sequence identity suggests this pathogen may represent a novel taxon in the genus and further requires whole genome sequencing for definitive taxonomic resolution. These findings provide insights on the natural occurrence of this novel pathogen of this invasive pest in Eucalyptus plantations in Brazil. Further studies are needed to determine potential of this microsporidium in the design of conservative or augmentative biological control programs for this invasive pest. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:01:16Z 2021-06-25T11:01:16Z 2021-12-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-021-90041-9 Scientific Reports, v. 11, n. 1, 2021. 2045-2322 http://hdl.handle.net/11449/207802 10.1038/s41598-021-90041-9 2-s2.0-85106918623 |
url |
http://dx.doi.org/10.1038/s41598-021-90041-9 http://hdl.handle.net/11449/207802 |
identifier_str_mv |
Scientific Reports, v. 11, n. 1, 2021. 2045-2322 10.1038/s41598-021-90041-9 2-s2.0-85106918623 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128130949316608 |