Reuse of norgestomet implants in an eCG-based superovulation protocol administered to Nelore (Bos taurus indicus) cows
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.livsci.2011.06.003 http://hdl.handle.net/11449/14699 |
Resumo: | This study assessed the reuse of norgestomet implants in Nelore cows that were superstimulated with eCG. In a crossover design trial, eight cows were randomly divided into two experimental groups and twice superstimulated: Group 1 - half of the cows received a new norgestomet implant and 2 mg estradiol benzoate (EB) on Day 0; Group 2 - remaining cows received two once-used norgestomet implants and 2 mg EB also on Day 0. on Day 4 all cows received a single dose of 2000 IU eCG, and on Day 6 cows were treated with two doses of PGF(2 alpha), 12 h apart. Ovulation was induced with 12.5 mg pLH 12 h after implant withdrawal (on Day 7), and fixed-time artificial inseminations were carried out 12 and 24 h later. Seven days after pLH injection embryos were recovered and blood samples were taken to determine circulating progesterone. Ultrasound examinations were performed at pLFI administration and at embryo recovery. The number of large follicles (>= 8 mm) was greater (P<0.05) in Group 1(17.1 +/- 1.8) than in Group 2 cows (9.7 +/- 1.6). The mean number of corporalutea was greater (P<0.05) for Group 1(13.8 +/- 1.8) as compared to Group 2 cows (5.4 +/- 1.0) and the percentage of large follicles that ovulated following pLH administration also differed (P<0.05) between treatments (80.3% and 53.8% for cows in Groups 1 and 2, respectively). However, no differences (P>0.05) were found between treatments (respectively, for Group 1 and Group 2 cows) in the follicle diameter (10.5 +/- 1.6 and 102 +/- 1.7 mm), corpus luteum (CL) diameter (15.7 +/- 1.8 and 14.4 +/- 1.7 mm), CL volume (2232 1356 and 1893 +/- 1828 mm(3)), ova/embryos recovered (6.3 +/- 1.1 and 4.0 +/- 1.9), transferable embryos (4.0 +/- 1.8 and 2.5 +/- 1.0) and plasma progesterone concentration (36.1 +/- 83 and 35.1 +/- 6.9 ng/mL). In conclusion, the stimulatory effects on the ovaries provided by the use of two once-used implants were less intense than the ones verified after using a new implant. (C) 2011 Elsevier B.V. All rights reserved. |
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Reuse of norgestomet implants in an eCG-based superovulation protocol administered to Nelore (Bos taurus indicus) cowsNorgestometSuperovulationeCGNeloreBos taurus indicusThis study assessed the reuse of norgestomet implants in Nelore cows that were superstimulated with eCG. In a crossover design trial, eight cows were randomly divided into two experimental groups and twice superstimulated: Group 1 - half of the cows received a new norgestomet implant and 2 mg estradiol benzoate (EB) on Day 0; Group 2 - remaining cows received two once-used norgestomet implants and 2 mg EB also on Day 0. on Day 4 all cows received a single dose of 2000 IU eCG, and on Day 6 cows were treated with two doses of PGF(2 alpha), 12 h apart. Ovulation was induced with 12.5 mg pLH 12 h after implant withdrawal (on Day 7), and fixed-time artificial inseminations were carried out 12 and 24 h later. Seven days after pLH injection embryos were recovered and blood samples were taken to determine circulating progesterone. Ultrasound examinations were performed at pLFI administration and at embryo recovery. The number of large follicles (>= 8 mm) was greater (P<0.05) in Group 1(17.1 +/- 1.8) than in Group 2 cows (9.7 +/- 1.6). The mean number of corporalutea was greater (P<0.05) for Group 1(13.8 +/- 1.8) as compared to Group 2 cows (5.4 +/- 1.0) and the percentage of large follicles that ovulated following pLH administration also differed (P<0.05) between treatments (80.3% and 53.8% for cows in Groups 1 and 2, respectively). However, no differences (P>0.05) were found between treatments (respectively, for Group 1 and Group 2 cows) in the follicle diameter (10.5 +/- 1.6 and 102 +/- 1.7 mm), corpus luteum (CL) diameter (15.7 +/- 1.8 and 14.4 +/- 1.7 mm), CL volume (2232 1356 and 1893 +/- 1828 mm(3)), ova/embryos recovered (6.3 +/- 1.1 and 4.0 +/- 1.9), transferable embryos (4.0 +/- 1.8 and 2.5 +/- 1.0) and plasma progesterone concentration (36.1 +/- 83 and 35.1 +/- 6.9 ng/mL). In conclusion, the stimulatory effects on the ovaries provided by the use of two once-used implants were less intense than the ones verified after using a new implant. (C) 2011 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)São Paulo State Univ UNESP, Sch Vet Med & Anim Sci FMVZ, Dept Anim Reprod & Vet Radiol, BR-18618970 Botucatu, SP, BrazilUniv São Paulo USP, Dept Anim Sci, BR-13418900 Piracicaba, SP, BrazilEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) SE Cattle, BR-13560970 São Carlos, SP, BrazilSão Paulo State Univ UNESP, Sch Vet Med & Anim Sci FMVZ, Dept Anim Reprod & Vet Radiol, BR-18618970 Botucatu, SP, BrazilFAPESP: 06/55199-2Elsevier B.V.Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Sudano, Mateus Jose [UNESP]Landim-Alvarenga, Fernanda da Cruz [UNESP]Sartori, RobertoMachado, Rui2013-09-30T18:29:07Z2014-05-20T13:42:17Z2013-09-30T18:29:07Z2014-05-20T13:42:17Z2011-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article207-212application/pdfhttp://dx.doi.org/10.1016/j.livsci.2011.06.003Livestock Science. Amsterdam: Elsevier B.V., v. 141, n. 2-3, p. 207-212, 2011.1871-1413http://hdl.handle.net/11449/1469910.1016/j.livsci.2011.06.003WOS:000296681000016WOS000296681000016.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLivestock Science1.2040,730info:eu-repo/semantics/openAccess2024-09-09T14:01:08Zoai:repositorio.unesp.br:11449/14699Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:01:08Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Reuse of norgestomet implants in an eCG-based superovulation protocol administered to Nelore (Bos taurus indicus) cows |
title |
Reuse of norgestomet implants in an eCG-based superovulation protocol administered to Nelore (Bos taurus indicus) cows |
spellingShingle |
Reuse of norgestomet implants in an eCG-based superovulation protocol administered to Nelore (Bos taurus indicus) cows Sudano, Mateus Jose [UNESP] Norgestomet Superovulation eCG Nelore Bos taurus indicus |
title_short |
Reuse of norgestomet implants in an eCG-based superovulation protocol administered to Nelore (Bos taurus indicus) cows |
title_full |
Reuse of norgestomet implants in an eCG-based superovulation protocol administered to Nelore (Bos taurus indicus) cows |
title_fullStr |
Reuse of norgestomet implants in an eCG-based superovulation protocol administered to Nelore (Bos taurus indicus) cows |
title_full_unstemmed |
Reuse of norgestomet implants in an eCG-based superovulation protocol administered to Nelore (Bos taurus indicus) cows |
title_sort |
Reuse of norgestomet implants in an eCG-based superovulation protocol administered to Nelore (Bos taurus indicus) cows |
author |
Sudano, Mateus Jose [UNESP] |
author_facet |
Sudano, Mateus Jose [UNESP] Landim-Alvarenga, Fernanda da Cruz [UNESP] Sartori, Roberto Machado, Rui |
author_role |
author |
author2 |
Landim-Alvarenga, Fernanda da Cruz [UNESP] Sartori, Roberto Machado, Rui |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Sudano, Mateus Jose [UNESP] Landim-Alvarenga, Fernanda da Cruz [UNESP] Sartori, Roberto Machado, Rui |
dc.subject.por.fl_str_mv |
Norgestomet Superovulation eCG Nelore Bos taurus indicus |
topic |
Norgestomet Superovulation eCG Nelore Bos taurus indicus |
description |
This study assessed the reuse of norgestomet implants in Nelore cows that were superstimulated with eCG. In a crossover design trial, eight cows were randomly divided into two experimental groups and twice superstimulated: Group 1 - half of the cows received a new norgestomet implant and 2 mg estradiol benzoate (EB) on Day 0; Group 2 - remaining cows received two once-used norgestomet implants and 2 mg EB also on Day 0. on Day 4 all cows received a single dose of 2000 IU eCG, and on Day 6 cows were treated with two doses of PGF(2 alpha), 12 h apart. Ovulation was induced with 12.5 mg pLH 12 h after implant withdrawal (on Day 7), and fixed-time artificial inseminations were carried out 12 and 24 h later. Seven days after pLH injection embryos were recovered and blood samples were taken to determine circulating progesterone. Ultrasound examinations were performed at pLFI administration and at embryo recovery. The number of large follicles (>= 8 mm) was greater (P<0.05) in Group 1(17.1 +/- 1.8) than in Group 2 cows (9.7 +/- 1.6). The mean number of corporalutea was greater (P<0.05) for Group 1(13.8 +/- 1.8) as compared to Group 2 cows (5.4 +/- 1.0) and the percentage of large follicles that ovulated following pLH administration also differed (P<0.05) between treatments (80.3% and 53.8% for cows in Groups 1 and 2, respectively). However, no differences (P>0.05) were found between treatments (respectively, for Group 1 and Group 2 cows) in the follicle diameter (10.5 +/- 1.6 and 102 +/- 1.7 mm), corpus luteum (CL) diameter (15.7 +/- 1.8 and 14.4 +/- 1.7 mm), CL volume (2232 1356 and 1893 +/- 1828 mm(3)), ova/embryos recovered (6.3 +/- 1.1 and 4.0 +/- 1.9), transferable embryos (4.0 +/- 1.8 and 2.5 +/- 1.0) and plasma progesterone concentration (36.1 +/- 83 and 35.1 +/- 6.9 ng/mL). In conclusion, the stimulatory effects on the ovaries provided by the use of two once-used implants were less intense than the ones verified after using a new implant. (C) 2011 Elsevier B.V. All rights reserved. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-11-01 2013-09-30T18:29:07Z 2013-09-30T18:29:07Z 2014-05-20T13:42:17Z 2014-05-20T13:42:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.livsci.2011.06.003 Livestock Science. Amsterdam: Elsevier B.V., v. 141, n. 2-3, p. 207-212, 2011. 1871-1413 http://hdl.handle.net/11449/14699 10.1016/j.livsci.2011.06.003 WOS:000296681000016 WOS000296681000016.pdf |
url |
http://dx.doi.org/10.1016/j.livsci.2011.06.003 http://hdl.handle.net/11449/14699 |
identifier_str_mv |
Livestock Science. Amsterdam: Elsevier B.V., v. 141, n. 2-3, p. 207-212, 2011. 1871-1413 10.1016/j.livsci.2011.06.003 WOS:000296681000016 WOS000296681000016.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Livestock Science 1.204 0,730 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
207-212 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546571182112768 |