Reuse of norgestomet implants in an eCG-based superovulation protocol administered to Nelore (Bos taurus indicus) cows

Detalhes bibliográficos
Autor(a) principal: Sudano, Mateus Jose [UNESP]
Data de Publicação: 2011
Outros Autores: Landim-Alvarenga, Fernanda da Cruz [UNESP], Sartori, Roberto, Machado, Rui
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.livsci.2011.06.003
http://hdl.handle.net/11449/14699
Resumo: This study assessed the reuse of norgestomet implants in Nelore cows that were superstimulated with eCG. In a crossover design trial, eight cows were randomly divided into two experimental groups and twice superstimulated: Group 1 - half of the cows received a new norgestomet implant and 2 mg estradiol benzoate (EB) on Day 0; Group 2 - remaining cows received two once-used norgestomet implants and 2 mg EB also on Day 0. on Day 4 all cows received a single dose of 2000 IU eCG, and on Day 6 cows were treated with two doses of PGF(2 alpha), 12 h apart. Ovulation was induced with 12.5 mg pLH 12 h after implant withdrawal (on Day 7), and fixed-time artificial inseminations were carried out 12 and 24 h later. Seven days after pLH injection embryos were recovered and blood samples were taken to determine circulating progesterone. Ultrasound examinations were performed at pLFI administration and at embryo recovery. The number of large follicles (>= 8 mm) was greater (P<0.05) in Group 1(17.1 +/- 1.8) than in Group 2 cows (9.7 +/- 1.6). The mean number of corporalutea was greater (P<0.05) for Group 1(13.8 +/- 1.8) as compared to Group 2 cows (5.4 +/- 1.0) and the percentage of large follicles that ovulated following pLH administration also differed (P<0.05) between treatments (80.3% and 53.8% for cows in Groups 1 and 2, respectively). However, no differences (P>0.05) were found between treatments (respectively, for Group 1 and Group 2 cows) in the follicle diameter (10.5 +/- 1.6 and 102 +/- 1.7 mm), corpus luteum (CL) diameter (15.7 +/- 1.8 and 14.4 +/- 1.7 mm), CL volume (2232 1356 and 1893 +/- 1828 mm(3)), ova/embryos recovered (6.3 +/- 1.1 and 4.0 +/- 1.9), transferable embryos (4.0 +/- 1.8 and 2.5 +/- 1.0) and plasma progesterone concentration (36.1 +/- 83 and 35.1 +/- 6.9 ng/mL). In conclusion, the stimulatory effects on the ovaries provided by the use of two once-used implants were less intense than the ones verified after using a new implant. (C) 2011 Elsevier B.V. All rights reserved.
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spelling Reuse of norgestomet implants in an eCG-based superovulation protocol administered to Nelore (Bos taurus indicus) cowsNorgestometSuperovulationeCGNeloreBos taurus indicusThis study assessed the reuse of norgestomet implants in Nelore cows that were superstimulated with eCG. In a crossover design trial, eight cows were randomly divided into two experimental groups and twice superstimulated: Group 1 - half of the cows received a new norgestomet implant and 2 mg estradiol benzoate (EB) on Day 0; Group 2 - remaining cows received two once-used norgestomet implants and 2 mg EB also on Day 0. on Day 4 all cows received a single dose of 2000 IU eCG, and on Day 6 cows were treated with two doses of PGF(2 alpha), 12 h apart. Ovulation was induced with 12.5 mg pLH 12 h after implant withdrawal (on Day 7), and fixed-time artificial inseminations were carried out 12 and 24 h later. Seven days after pLH injection embryos were recovered and blood samples were taken to determine circulating progesterone. Ultrasound examinations were performed at pLFI administration and at embryo recovery. The number of large follicles (>= 8 mm) was greater (P<0.05) in Group 1(17.1 +/- 1.8) than in Group 2 cows (9.7 +/- 1.6). The mean number of corporalutea was greater (P<0.05) for Group 1(13.8 +/- 1.8) as compared to Group 2 cows (5.4 +/- 1.0) and the percentage of large follicles that ovulated following pLH administration also differed (P<0.05) between treatments (80.3% and 53.8% for cows in Groups 1 and 2, respectively). However, no differences (P>0.05) were found between treatments (respectively, for Group 1 and Group 2 cows) in the follicle diameter (10.5 +/- 1.6 and 102 +/- 1.7 mm), corpus luteum (CL) diameter (15.7 +/- 1.8 and 14.4 +/- 1.7 mm), CL volume (2232 1356 and 1893 +/- 1828 mm(3)), ova/embryos recovered (6.3 +/- 1.1 and 4.0 +/- 1.9), transferable embryos (4.0 +/- 1.8 and 2.5 +/- 1.0) and plasma progesterone concentration (36.1 +/- 83 and 35.1 +/- 6.9 ng/mL). In conclusion, the stimulatory effects on the ovaries provided by the use of two once-used implants were less intense than the ones verified after using a new implant. (C) 2011 Elsevier B.V. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)São Paulo State Univ UNESP, Sch Vet Med & Anim Sci FMVZ, Dept Anim Reprod & Vet Radiol, BR-18618970 Botucatu, SP, BrazilUniv São Paulo USP, Dept Anim Sci, BR-13418900 Piracicaba, SP, BrazilEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA) SE Cattle, BR-13560970 São Carlos, SP, BrazilSão Paulo State Univ UNESP, Sch Vet Med & Anim Sci FMVZ, Dept Anim Reprod & Vet Radiol, BR-18618970 Botucatu, SP, BrazilFAPESP: 06/55199-2Elsevier B.V.Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Sudano, Mateus Jose [UNESP]Landim-Alvarenga, Fernanda da Cruz [UNESP]Sartori, RobertoMachado, Rui2013-09-30T18:29:07Z2014-05-20T13:42:17Z2013-09-30T18:29:07Z2014-05-20T13:42:17Z2011-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article207-212application/pdfhttp://dx.doi.org/10.1016/j.livsci.2011.06.003Livestock Science. Amsterdam: Elsevier B.V., v. 141, n. 2-3, p. 207-212, 2011.1871-1413http://hdl.handle.net/11449/1469910.1016/j.livsci.2011.06.003WOS:000296681000016WOS000296681000016.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLivestock Science1.2040,730info:eu-repo/semantics/openAccess2024-09-09T14:01:08Zoai:repositorio.unesp.br:11449/14699Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:01:08Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Reuse of norgestomet implants in an eCG-based superovulation protocol administered to Nelore (Bos taurus indicus) cows
title Reuse of norgestomet implants in an eCG-based superovulation protocol administered to Nelore (Bos taurus indicus) cows
spellingShingle Reuse of norgestomet implants in an eCG-based superovulation protocol administered to Nelore (Bos taurus indicus) cows
Sudano, Mateus Jose [UNESP]
Norgestomet
Superovulation
eCG
Nelore
Bos taurus indicus
title_short Reuse of norgestomet implants in an eCG-based superovulation protocol administered to Nelore (Bos taurus indicus) cows
title_full Reuse of norgestomet implants in an eCG-based superovulation protocol administered to Nelore (Bos taurus indicus) cows
title_fullStr Reuse of norgestomet implants in an eCG-based superovulation protocol administered to Nelore (Bos taurus indicus) cows
title_full_unstemmed Reuse of norgestomet implants in an eCG-based superovulation protocol administered to Nelore (Bos taurus indicus) cows
title_sort Reuse of norgestomet implants in an eCG-based superovulation protocol administered to Nelore (Bos taurus indicus) cows
author Sudano, Mateus Jose [UNESP]
author_facet Sudano, Mateus Jose [UNESP]
Landim-Alvarenga, Fernanda da Cruz [UNESP]
Sartori, Roberto
Machado, Rui
author_role author
author2 Landim-Alvarenga, Fernanda da Cruz [UNESP]
Sartori, Roberto
Machado, Rui
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.author.fl_str_mv Sudano, Mateus Jose [UNESP]
Landim-Alvarenga, Fernanda da Cruz [UNESP]
Sartori, Roberto
Machado, Rui
dc.subject.por.fl_str_mv Norgestomet
Superovulation
eCG
Nelore
Bos taurus indicus
topic Norgestomet
Superovulation
eCG
Nelore
Bos taurus indicus
description This study assessed the reuse of norgestomet implants in Nelore cows that were superstimulated with eCG. In a crossover design trial, eight cows were randomly divided into two experimental groups and twice superstimulated: Group 1 - half of the cows received a new norgestomet implant and 2 mg estradiol benzoate (EB) on Day 0; Group 2 - remaining cows received two once-used norgestomet implants and 2 mg EB also on Day 0. on Day 4 all cows received a single dose of 2000 IU eCG, and on Day 6 cows were treated with two doses of PGF(2 alpha), 12 h apart. Ovulation was induced with 12.5 mg pLH 12 h after implant withdrawal (on Day 7), and fixed-time artificial inseminations were carried out 12 and 24 h later. Seven days after pLH injection embryos were recovered and blood samples were taken to determine circulating progesterone. Ultrasound examinations were performed at pLFI administration and at embryo recovery. The number of large follicles (>= 8 mm) was greater (P<0.05) in Group 1(17.1 +/- 1.8) than in Group 2 cows (9.7 +/- 1.6). The mean number of corporalutea was greater (P<0.05) for Group 1(13.8 +/- 1.8) as compared to Group 2 cows (5.4 +/- 1.0) and the percentage of large follicles that ovulated following pLH administration also differed (P<0.05) between treatments (80.3% and 53.8% for cows in Groups 1 and 2, respectively). However, no differences (P>0.05) were found between treatments (respectively, for Group 1 and Group 2 cows) in the follicle diameter (10.5 +/- 1.6 and 102 +/- 1.7 mm), corpus luteum (CL) diameter (15.7 +/- 1.8 and 14.4 +/- 1.7 mm), CL volume (2232 1356 and 1893 +/- 1828 mm(3)), ova/embryos recovered (6.3 +/- 1.1 and 4.0 +/- 1.9), transferable embryos (4.0 +/- 1.8 and 2.5 +/- 1.0) and plasma progesterone concentration (36.1 +/- 83 and 35.1 +/- 6.9 ng/mL). In conclusion, the stimulatory effects on the ovaries provided by the use of two once-used implants were less intense than the ones verified after using a new implant. (C) 2011 Elsevier B.V. All rights reserved.
publishDate 2011
dc.date.none.fl_str_mv 2011-11-01
2013-09-30T18:29:07Z
2013-09-30T18:29:07Z
2014-05-20T13:42:17Z
2014-05-20T13:42:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.livsci.2011.06.003
Livestock Science. Amsterdam: Elsevier B.V., v. 141, n. 2-3, p. 207-212, 2011.
1871-1413
http://hdl.handle.net/11449/14699
10.1016/j.livsci.2011.06.003
WOS:000296681000016
WOS000296681000016.pdf
url http://dx.doi.org/10.1016/j.livsci.2011.06.003
http://hdl.handle.net/11449/14699
identifier_str_mv Livestock Science. Amsterdam: Elsevier B.V., v. 141, n. 2-3, p. 207-212, 2011.
1871-1413
10.1016/j.livsci.2011.06.003
WOS:000296681000016
WOS000296681000016.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Livestock Science
1.204
0,730
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 207-212
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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