Investigation of prognostic value of claudin-5, psma, and ki67 expression in canine splenic hemangiosarcoma
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/ani11082406 http://hdl.handle.net/11449/222190 |
Resumo: | Splenic hemangiosarcoma (HSA) is a malignant tumor of endothelial cells that affects middle-aged and elderly dogs and is characterized by the formation of new blood vessels, commonly associated with necrotic and hemorrhagic areas. Despite its importance in veterinary medicine, few studies have identified markers with prognostic value for canine HSA. Thus, this study aimed to associate the clinicopathological findings (prostate-specific membrane antigen [PSMA], Claudin-5, and Ki67 gene and protein expression) with overall survival in HSA-affected patients. Fifty-three formalin-fixed and paraffin-embedded canine splenic HSA samples, previously diagnosed by histopathological examination, were used in this study. Claudin-5, PSMA, and Ki67 protein expression levels were evaluated by immunohistochemistry, and gene expression was evaluated by quantitative polymerase chain reaction. Claudin-5 protein overexpression was observed in patients with metastasis (p = 0.0078) and with stage III tumors compared to those with stage I and II tumors (p = 0.0451). In patients treated with surgery alone, low PSMA gene and protein expression (p = 0.05 and p = 0.0355, respectively) were associated with longer survival time. Longer survival time was observed in patients with a low Ki67 index (p = 0.0488). Our results indicate that Claudin-5 protein expression is associated with metastatic status, and PSMA gene and protein expression, and Ki67 index are associated with survival time. |
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Investigation of prognostic value of claudin-5, psma, and ki67 expression in canine splenic hemangiosarcomaCanine spleen neoplasiaEndothelial splenic neoplasiaKi67Proliferative indexProstate-specific membrane antigenSplenic hemangiosarcoma (HSA) is a malignant tumor of endothelial cells that affects middle-aged and elderly dogs and is characterized by the formation of new blood vessels, commonly associated with necrotic and hemorrhagic areas. Despite its importance in veterinary medicine, few studies have identified markers with prognostic value for canine HSA. Thus, this study aimed to associate the clinicopathological findings (prostate-specific membrane antigen [PSMA], Claudin-5, and Ki67 gene and protein expression) with overall survival in HSA-affected patients. Fifty-three formalin-fixed and paraffin-embedded canine splenic HSA samples, previously diagnosed by histopathological examination, were used in this study. Claudin-5, PSMA, and Ki67 protein expression levels were evaluated by immunohistochemistry, and gene expression was evaluated by quantitative polymerase chain reaction. Claudin-5 protein overexpression was observed in patients with metastasis (p = 0.0078) and with stage III tumors compared to those with stage I and II tumors (p = 0.0451). In patients treated with surgery alone, low PSMA gene and protein expression (p = 0.05 and p = 0.0355, respectively) were associated with longer survival time. Longer survival time was observed in patients with a low Ki67 index (p = 0.0488). Our results indicate that Claudin-5 protein expression is associated with metastatic status, and PSMA gene and protein expression, and Ki67 index are associated with survival time.Department of Veterinary Surgery and Animal Reproduction Sao Paulo State University—UNESPDepartment of Veterinary Clinic Sao Paulo State University—UNESPONCO CANE-VeterinaryVetPat Veterinary LaboratoryVeterinary School Federal University of Fronteira Sul—UFFSInstitute of Health Sciences Paulista University—UNIPDepartment of Veterinary Surgery and Animal Reproduction Sao Paulo State University—UNESPDepartment of Veterinary Clinic Sao Paulo State University—UNESPUniversidade Estadual Paulista (UNESP)ONCO CANE-VeterinaryVetPat Veterinary LaboratoryFederal University of Fronteira Sul—UFFSPaulista University—UNIPRozolen, Juliana Moreira [UNESP]Teodoro, Tamires Goneli Wichert [UNESP]Sobral, Renata AfonsoSueiro, Felipe Augusto RuizLaufer-Amorim, Renee [UNESP]Elias, FabianaFonseca-Alves, Carlos Eduardo [UNESP]2022-04-28T19:43:11Z2022-04-28T19:43:11Z2021-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ani11082406Animals, v. 11, n. 8, 2021.2076-2615http://hdl.handle.net/11449/22219010.3390/ani110824062-s2.0-85112396446Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAnimalsinfo:eu-repo/semantics/openAccess2022-04-28T19:43:11Zoai:repositorio.unesp.br:11449/222190Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:28:27.286800Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Investigation of prognostic value of claudin-5, psma, and ki67 expression in canine splenic hemangiosarcoma |
title |
Investigation of prognostic value of claudin-5, psma, and ki67 expression in canine splenic hemangiosarcoma |
spellingShingle |
Investigation of prognostic value of claudin-5, psma, and ki67 expression in canine splenic hemangiosarcoma Rozolen, Juliana Moreira [UNESP] Canine spleen neoplasia Endothelial splenic neoplasia Ki67 Proliferative index Prostate-specific membrane antigen |
title_short |
Investigation of prognostic value of claudin-5, psma, and ki67 expression in canine splenic hemangiosarcoma |
title_full |
Investigation of prognostic value of claudin-5, psma, and ki67 expression in canine splenic hemangiosarcoma |
title_fullStr |
Investigation of prognostic value of claudin-5, psma, and ki67 expression in canine splenic hemangiosarcoma |
title_full_unstemmed |
Investigation of prognostic value of claudin-5, psma, and ki67 expression in canine splenic hemangiosarcoma |
title_sort |
Investigation of prognostic value of claudin-5, psma, and ki67 expression in canine splenic hemangiosarcoma |
author |
Rozolen, Juliana Moreira [UNESP] |
author_facet |
Rozolen, Juliana Moreira [UNESP] Teodoro, Tamires Goneli Wichert [UNESP] Sobral, Renata Afonso Sueiro, Felipe Augusto Ruiz Laufer-Amorim, Renee [UNESP] Elias, Fabiana Fonseca-Alves, Carlos Eduardo [UNESP] |
author_role |
author |
author2 |
Teodoro, Tamires Goneli Wichert [UNESP] Sobral, Renata Afonso Sueiro, Felipe Augusto Ruiz Laufer-Amorim, Renee [UNESP] Elias, Fabiana Fonseca-Alves, Carlos Eduardo [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) ONCO CANE-Veterinary VetPat Veterinary Laboratory Federal University of Fronteira Sul—UFFS Paulista University—UNIP |
dc.contributor.author.fl_str_mv |
Rozolen, Juliana Moreira [UNESP] Teodoro, Tamires Goneli Wichert [UNESP] Sobral, Renata Afonso Sueiro, Felipe Augusto Ruiz Laufer-Amorim, Renee [UNESP] Elias, Fabiana Fonseca-Alves, Carlos Eduardo [UNESP] |
dc.subject.por.fl_str_mv |
Canine spleen neoplasia Endothelial splenic neoplasia Ki67 Proliferative index Prostate-specific membrane antigen |
topic |
Canine spleen neoplasia Endothelial splenic neoplasia Ki67 Proliferative index Prostate-specific membrane antigen |
description |
Splenic hemangiosarcoma (HSA) is a malignant tumor of endothelial cells that affects middle-aged and elderly dogs and is characterized by the formation of new blood vessels, commonly associated with necrotic and hemorrhagic areas. Despite its importance in veterinary medicine, few studies have identified markers with prognostic value for canine HSA. Thus, this study aimed to associate the clinicopathological findings (prostate-specific membrane antigen [PSMA], Claudin-5, and Ki67 gene and protein expression) with overall survival in HSA-affected patients. Fifty-three formalin-fixed and paraffin-embedded canine splenic HSA samples, previously diagnosed by histopathological examination, were used in this study. Claudin-5, PSMA, and Ki67 protein expression levels were evaluated by immunohistochemistry, and gene expression was evaluated by quantitative polymerase chain reaction. Claudin-5 protein overexpression was observed in patients with metastasis (p = 0.0078) and with stage III tumors compared to those with stage I and II tumors (p = 0.0451). In patients treated with surgery alone, low PSMA gene and protein expression (p = 0.05 and p = 0.0355, respectively) were associated with longer survival time. Longer survival time was observed in patients with a low Ki67 index (p = 0.0488). Our results indicate that Claudin-5 protein expression is associated with metastatic status, and PSMA gene and protein expression, and Ki67 index are associated with survival time. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-01 2022-04-28T19:43:11Z 2022-04-28T19:43:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ani11082406 Animals, v. 11, n. 8, 2021. 2076-2615 http://hdl.handle.net/11449/222190 10.3390/ani11082406 2-s2.0-85112396446 |
url |
http://dx.doi.org/10.3390/ani11082406 http://hdl.handle.net/11449/222190 |
identifier_str_mv |
Animals, v. 11, n. 8, 2021. 2076-2615 10.3390/ani11082406 2-s2.0-85112396446 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Animals |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128816338436096 |