Eletroforese das proteínas séricas e urinárias de cães com erliquiose na fase aguda

Detalhes bibliográficos
Autor(a) principal: Contin, Catarina Micali [UNESP]
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/128029
http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/18-09-2015/000848474.pdf
Resumo: Canine ehrlichiosis is one of the most frequent haemoparasitosis between domestic dogs and has high prevalence in tropical regions. Despite the innumerous investigations and progresses in the pathogenesis comprehension and diagnosis of the disease, little is known on the glomerulo-tubular alterations of dogs stricken by ehrlichiosis. The aim of this study was to associate the electrophoretic profile of blood and urinary proteins with the possible glomerulo-tubular alterations found in dogs with acute-phase ehrlichiosis. The Control Group (CG) was formed by 18 healthy dogs and the Sick Group (SG), by 24 animals, both groups with unspecified genders, races and ages. The experimental protocol included assessment of the dogs' clinical conditions, and regarding the sick ones, based on the clinical signs and the presence of morula in the ear-tip blood smears. From the animals of both groups, peripheral blood and urine samples were obtained for protein electrophoretical analysis through the polyacrylamide gel with dodecyl sodium sulfate (SDS-PAGE) electrophoresis technique. Eleven protein fractions were selected in the blood sera densitometry trace, with molecular weights ranging from 23 to 123 kDa (kilodaltons). The results showed that some acute-phase proteins change in dogs with acute-phase ehrlichiosis. Significant difference between the studied groups was observed in serum concentrations of transferrin, haptoglobin, IgG (light chain) and albumin, the latter with lower levels in the Sick Group. The electophoretical analysis of urine showed a larger number of protein fractions excreted by dogs with acute-phase ehrlichiosis (295), when in comparison with the ones in the CG (174). High (>60 kDa) and low (<60 kDa) molecular weight proteins were found in the sick dogs' urine, suggesting that the disease in its acute phase causes lesions in both glomeruli and tubules, respectively
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spelling Eletroforese das proteínas séricas e urinárias de cães com erliquiose na fase agudaCão - DoençasGlomerulos renaisProteinúriaErliquioseProteínasEhrlichiosisCanine ehrlichiosis is one of the most frequent haemoparasitosis between domestic dogs and has high prevalence in tropical regions. Despite the innumerous investigations and progresses in the pathogenesis comprehension and diagnosis of the disease, little is known on the glomerulo-tubular alterations of dogs stricken by ehrlichiosis. The aim of this study was to associate the electrophoretic profile of blood and urinary proteins with the possible glomerulo-tubular alterations found in dogs with acute-phase ehrlichiosis. The Control Group (CG) was formed by 18 healthy dogs and the Sick Group (SG), by 24 animals, both groups with unspecified genders, races and ages. The experimental protocol included assessment of the dogs' clinical conditions, and regarding the sick ones, based on the clinical signs and the presence of morula in the ear-tip blood smears. From the animals of both groups, peripheral blood and urine samples were obtained for protein electrophoretical analysis through the polyacrylamide gel with dodecyl sodium sulfate (SDS-PAGE) electrophoresis technique. Eleven protein fractions were selected in the blood sera densitometry trace, with molecular weights ranging from 23 to 123 kDa (kilodaltons). The results showed that some acute-phase proteins change in dogs with acute-phase ehrlichiosis. Significant difference between the studied groups was observed in serum concentrations of transferrin, haptoglobin, IgG (light chain) and albumin, the latter with lower levels in the Sick Group. The electophoretical analysis of urine showed a larger number of protein fractions excreted by dogs with acute-phase ehrlichiosis (295), when in comparison with the ones in the CG (174). High (>60 kDa) and low (<60 kDa) molecular weight proteins were found in the sick dogs' urine, suggesting that the disease in its acute phase causes lesions in both glomeruli and tubules, respectivelyA erliquiose canina é uma das hemoparasitoses mais frequentes entre os cães domésticos e apresenta alta prevalência nas regiões de clima tropical. Apesar das inúmeras investigações e avanços na compreensão da patogênese e diagnóstico da referida doença, pouco se sabe a respeito das alterações glomérulo-tubulares de cães acometidos por erliquiose. No presente estudo objetivou-se associar o perfil eletroforético das proteínas sanguíneas e urinárias com as possíveis alterações glomérulo-tubulares encontradas em cães com erliquiose na fase aguda. O Grupo Controle (GC) foi integrado por 18 cães saudáveis e o Grupo Doente (GD), por 24 animais. Ambos de qualquer gênero, raça e idade. O protocolo experimental incluiu avaliação das condições clínicas de cães sadios e daqueles acometidos pela doença, neste último caso com base nos sinais clínicos e presença de mórula em esfregaços sanguíneos de ponta de orelha. Dos animais controle e daqueles enfermos, foram obtidas amostras de sangue periférico e urina para análises eletroforéticas das proteínas pela técnica de eletroforese em gel de poliacrilamida contendo dodecil sulfato de sódio (SDS-PAGE). Foram selecionadas 11 frações proteicas no traçado densitométrico dos soros sanguíneos, cujos pesos moleculares variaram entre 23 e 139 kDa (kilodáltons). Os resultados mostraram que algumas proteínas séricas de fase aguda se alteram nos cães com erliquiose na fase aguda. Observou-se diferença significativa entre os grupos estudados na concentração sérica de transferrina, haptoglobina, IgG (cadeia leve) e albumina, sendo que somente esta última apresentou níveis inferiores no Grupo Doente. A análise eletroforética da urina exibiu um número superior de frações proteicas excretadas pelos cães com erliquiose na fase aguda (295), quando comparados àqueles encontrados nos cães do GC (174). Foram encontradas proteínas de alto (>60 kDa) e baixo...Universidade Estadual Paulista (Unesp)Santana, Aureo Evangelista [UNESP]Universidade Estadual Paulista (Unesp)Contin, Catarina Micali [UNESP]2015-10-06T13:02:56Z2015-10-06T13:02:56Z2015-07-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisx, 68 p. : il.application/pdfCONTIN, Catarina Micali. Eletroforese das proteínas séricas e urinárias de cães com erliquiose na fase aguda. 2015. x, 68 p. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, 2015.http://hdl.handle.net/11449/128029000848474http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/18-09-2015/000848474.pdf33004102072P98905659987198068Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2024-06-05T13:49:22Zoai:repositorio.unesp.br:11449/128029Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:00:59.240042Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Eletroforese das proteínas séricas e urinárias de cães com erliquiose na fase aguda
title Eletroforese das proteínas séricas e urinárias de cães com erliquiose na fase aguda
spellingShingle Eletroforese das proteínas séricas e urinárias de cães com erliquiose na fase aguda
Contin, Catarina Micali [UNESP]
Cão - Doenças
Glomerulos renais
Proteinúria
Erliquiose
Proteínas
Ehrlichiosis
title_short Eletroforese das proteínas séricas e urinárias de cães com erliquiose na fase aguda
title_full Eletroforese das proteínas séricas e urinárias de cães com erliquiose na fase aguda
title_fullStr Eletroforese das proteínas séricas e urinárias de cães com erliquiose na fase aguda
title_full_unstemmed Eletroforese das proteínas séricas e urinárias de cães com erliquiose na fase aguda
title_sort Eletroforese das proteínas séricas e urinárias de cães com erliquiose na fase aguda
author Contin, Catarina Micali [UNESP]
author_facet Contin, Catarina Micali [UNESP]
author_role author
dc.contributor.none.fl_str_mv Santana, Aureo Evangelista [UNESP]
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Contin, Catarina Micali [UNESP]
dc.subject.por.fl_str_mv Cão - Doenças
Glomerulos renais
Proteinúria
Erliquiose
Proteínas
Ehrlichiosis
topic Cão - Doenças
Glomerulos renais
Proteinúria
Erliquiose
Proteínas
Ehrlichiosis
description Canine ehrlichiosis is one of the most frequent haemoparasitosis between domestic dogs and has high prevalence in tropical regions. Despite the innumerous investigations and progresses in the pathogenesis comprehension and diagnosis of the disease, little is known on the glomerulo-tubular alterations of dogs stricken by ehrlichiosis. The aim of this study was to associate the electrophoretic profile of blood and urinary proteins with the possible glomerulo-tubular alterations found in dogs with acute-phase ehrlichiosis. The Control Group (CG) was formed by 18 healthy dogs and the Sick Group (SG), by 24 animals, both groups with unspecified genders, races and ages. The experimental protocol included assessment of the dogs' clinical conditions, and regarding the sick ones, based on the clinical signs and the presence of morula in the ear-tip blood smears. From the animals of both groups, peripheral blood and urine samples were obtained for protein electrophoretical analysis through the polyacrylamide gel with dodecyl sodium sulfate (SDS-PAGE) electrophoresis technique. Eleven protein fractions were selected in the blood sera densitometry trace, with molecular weights ranging from 23 to 123 kDa (kilodaltons). The results showed that some acute-phase proteins change in dogs with acute-phase ehrlichiosis. Significant difference between the studied groups was observed in serum concentrations of transferrin, haptoglobin, IgG (light chain) and albumin, the latter with lower levels in the Sick Group. The electophoretical analysis of urine showed a larger number of protein fractions excreted by dogs with acute-phase ehrlichiosis (295), when in comparison with the ones in the CG (174). High (>60 kDa) and low (<60 kDa) molecular weight proteins were found in the sick dogs' urine, suggesting that the disease in its acute phase causes lesions in both glomeruli and tubules, respectively
publishDate 2015
dc.date.none.fl_str_mv 2015-10-06T13:02:56Z
2015-10-06T13:02:56Z
2015-07-02
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv CONTIN, Catarina Micali. Eletroforese das proteínas séricas e urinárias de cães com erliquiose na fase aguda. 2015. x, 68 p. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, 2015.
http://hdl.handle.net/11449/128029
000848474
http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/18-09-2015/000848474.pdf
33004102072P9
8905659987198068
identifier_str_mv CONTIN, Catarina Micali. Eletroforese das proteínas séricas e urinárias de cães com erliquiose na fase aguda. 2015. x, 68 p. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, 2015.
000848474
33004102072P9
8905659987198068
url http://hdl.handle.net/11449/128029
http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/18-09-2015/000848474.pdf
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dc.format.none.fl_str_mv x, 68 p. : il.
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publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.source.none.fl_str_mv Aleph
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
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instname_str Universidade Estadual Paulista (UNESP)
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