Cylindrospermopsin Disrupts Estrous Cycle and Increases Spermatogenesis in Mice
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s43032-022-00907-0 http://hdl.handle.net/11449/234230 |
Resumo: | Cylindrospermopsin (CYN) is a water-soluble cyanotoxin that has been linked to several cases of poisoning in the world. In vitro studies have shown that CYN acts as an endocrine disruptor by inhibiting progesterone synthesis in primary cell cultures of women, showing estrogenic activity. However, in vivo assessment of CYN in the female and male reproductive systems remains unknown. We thus aimed to evaluate the in vivo effects of CYN in both the female and male reproductive systems of mice. A single intraperitoneal exposure to 64 µg of CYN/kg body weight was performed in females. Estrous cycle was evaluated daily by vaginal cytology, and serum progesterone and estradiol levels were measured after 50 days. We showed an impairment in the estrous cycle as well as a decrease in circulating plasma progesterone levels. In males, weekly intraperitoneal doses of 20 μg of CYN/kg body weight were given and groups were killed after one, two, or four doses. CYN increased the testosterone levels in the groups that received one or two doses of CYN. Additionally, CYN induced a transient increase in spermatozoa in males after four doses. Our results highlight that CYN interferes with both male and female reproductive systems and may lead to infertility. As far as we know, this is the first report showing the impacts of CYN on the mammalian reproductive system, suggesting a threat from this cyanotoxin to human and environmental health. |
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Cylindrospermopsin Disrupts Estrous Cycle and Increases Spermatogenesis in MiceCyanotoxinsEndocrine disruptorIn vivoReproductive toxicologyCylindrospermopsin (CYN) is a water-soluble cyanotoxin that has been linked to several cases of poisoning in the world. In vitro studies have shown that CYN acts as an endocrine disruptor by inhibiting progesterone synthesis in primary cell cultures of women, showing estrogenic activity. However, in vivo assessment of CYN in the female and male reproductive systems remains unknown. We thus aimed to evaluate the in vivo effects of CYN in both the female and male reproductive systems of mice. A single intraperitoneal exposure to 64 µg of CYN/kg body weight was performed in females. Estrous cycle was evaluated daily by vaginal cytology, and serum progesterone and estradiol levels were measured after 50 days. We showed an impairment in the estrous cycle as well as a decrease in circulating plasma progesterone levels. In males, weekly intraperitoneal doses of 20 μg of CYN/kg body weight were given and groups were killed after one, two, or four doses. CYN increased the testosterone levels in the groups that received one or two doses of CYN. Additionally, CYN induced a transient increase in spermatozoa in males after four doses. Our results highlight that CYN interferes with both male and female reproductive systems and may lead to infertility. As far as we know, this is the first report showing the impacts of CYN on the mammalian reproductive system, suggesting a threat from this cyanotoxin to human and environmental health.Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)Laboratory of Ecophysiology and Toxicology of Cyanobacteria Institute of Biophysics Carlos Chagas Filho UFRJ, RJLaboratory of Translational Endocrinology Institute of Biophysics Carlos Chagas Filho UFRJ, RJReproductive and Molecular Biology Group Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), São PauloReproductive and Molecular Biology Group Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP), São PauloUniversidade Federal do Rio de Janeiro (UFRJ)Universidade Estadual Paulista (UNESP)de Moraes, Adriana Carvalho NatalCaires, Fernanda OliveiraImperio, Guinever EustaquioNóbrega, Rafael Henrique [UNESP]Ortiga-Carvalho, Tania Mariade Magalhães, Valéria Freitas2022-05-01T15:13:34Z2022-05-01T15:13:34Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s43032-022-00907-0Reproductive Sciences.1933-72051933-7191http://hdl.handle.net/11449/23423010.1007/s43032-022-00907-02-s2.0-85125738152Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengReproductive Sciencesinfo:eu-repo/semantics/openAccess2022-05-01T15:13:34Zoai:repositorio.unesp.br:11449/234230Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-05-01T15:13:34Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cylindrospermopsin Disrupts Estrous Cycle and Increases Spermatogenesis in Mice |
title |
Cylindrospermopsin Disrupts Estrous Cycle and Increases Spermatogenesis in Mice |
spellingShingle |
Cylindrospermopsin Disrupts Estrous Cycle and Increases Spermatogenesis in Mice de Moraes, Adriana Carvalho Natal Cyanotoxins Endocrine disruptor In vivo Reproductive toxicology |
title_short |
Cylindrospermopsin Disrupts Estrous Cycle and Increases Spermatogenesis in Mice |
title_full |
Cylindrospermopsin Disrupts Estrous Cycle and Increases Spermatogenesis in Mice |
title_fullStr |
Cylindrospermopsin Disrupts Estrous Cycle and Increases Spermatogenesis in Mice |
title_full_unstemmed |
Cylindrospermopsin Disrupts Estrous Cycle and Increases Spermatogenesis in Mice |
title_sort |
Cylindrospermopsin Disrupts Estrous Cycle and Increases Spermatogenesis in Mice |
author |
de Moraes, Adriana Carvalho Natal |
author_facet |
de Moraes, Adriana Carvalho Natal Caires, Fernanda Oliveira Imperio, Guinever Eustaquio Nóbrega, Rafael Henrique [UNESP] Ortiga-Carvalho, Tania Maria de Magalhães, Valéria Freitas |
author_role |
author |
author2 |
Caires, Fernanda Oliveira Imperio, Guinever Eustaquio Nóbrega, Rafael Henrique [UNESP] Ortiga-Carvalho, Tania Maria de Magalhães, Valéria Freitas |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Rio de Janeiro (UFRJ) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
de Moraes, Adriana Carvalho Natal Caires, Fernanda Oliveira Imperio, Guinever Eustaquio Nóbrega, Rafael Henrique [UNESP] Ortiga-Carvalho, Tania Maria de Magalhães, Valéria Freitas |
dc.subject.por.fl_str_mv |
Cyanotoxins Endocrine disruptor In vivo Reproductive toxicology |
topic |
Cyanotoxins Endocrine disruptor In vivo Reproductive toxicology |
description |
Cylindrospermopsin (CYN) is a water-soluble cyanotoxin that has been linked to several cases of poisoning in the world. In vitro studies have shown that CYN acts as an endocrine disruptor by inhibiting progesterone synthesis in primary cell cultures of women, showing estrogenic activity. However, in vivo assessment of CYN in the female and male reproductive systems remains unknown. We thus aimed to evaluate the in vivo effects of CYN in both the female and male reproductive systems of mice. A single intraperitoneal exposure to 64 µg of CYN/kg body weight was performed in females. Estrous cycle was evaluated daily by vaginal cytology, and serum progesterone and estradiol levels were measured after 50 days. We showed an impairment in the estrous cycle as well as a decrease in circulating plasma progesterone levels. In males, weekly intraperitoneal doses of 20 μg of CYN/kg body weight were given and groups were killed after one, two, or four doses. CYN increased the testosterone levels in the groups that received one or two doses of CYN. Additionally, CYN induced a transient increase in spermatozoa in males after four doses. Our results highlight that CYN interferes with both male and female reproductive systems and may lead to infertility. As far as we know, this is the first report showing the impacts of CYN on the mammalian reproductive system, suggesting a threat from this cyanotoxin to human and environmental health. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-01T15:13:34Z 2022-05-01T15:13:34Z 2022-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s43032-022-00907-0 Reproductive Sciences. 1933-7205 1933-7191 http://hdl.handle.net/11449/234230 10.1007/s43032-022-00907-0 2-s2.0-85125738152 |
url |
http://dx.doi.org/10.1007/s43032-022-00907-0 http://hdl.handle.net/11449/234230 |
identifier_str_mv |
Reproductive Sciences. 1933-7205 1933-7191 10.1007/s43032-022-00907-0 2-s2.0-85125738152 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Reproductive Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965587860881408 |