Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattle
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
DOI: | 10.1590/S0074-02762001000200017 |
Texto Completo: | http://dx.doi.org/10.1590/S0074-02762001000200017 http://hdl.handle.net/11449/27207 |
Resumo: | A crude antigenic preparation of Babesia bigemina was used to develop an ELISA for the detection of IgM antibodies. Optimal dilutions of the antigen, using positive and negative reference sera, were determined by checkerboard titrations. Negative sera from cattle imported from tick-free areas, serum samples collected from infected B. bigemina cattle were used to validate the test. The specificity was 94% and sensitivity of the Elisa 87.5%. Sera from 385 cattle deriving from areas free from tick-borne diseases, which were submitted to a preimmunization process, were screened by this technique. The Elisa detected seroconversion on the 14th day post-inoculation in animals either infested with Boophilus microplus ticks (infected with B. bigemina), or inoculated with B. bigemina infected blood. Antibody titers decreased after day 33; however, all animals remained positive until the end of the experiment (124 days). The ELISA described may prove to be an appropriate serological test for the detection of IgM antibodies against B. bigemina. |
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Repositório Institucional da UNESP |
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spelling |
Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattleBabesia bigeminaELISAIgMA crude antigenic preparation of Babesia bigemina was used to develop an ELISA for the detection of IgM antibodies. Optimal dilutions of the antigen, using positive and negative reference sera, were determined by checkerboard titrations. Negative sera from cattle imported from tick-free areas, serum samples collected from infected B. bigemina cattle were used to validate the test. The specificity was 94% and sensitivity of the Elisa 87.5%. Sera from 385 cattle deriving from areas free from tick-borne diseases, which were submitted to a preimmunization process, were screened by this technique. The Elisa detected seroconversion on the 14th day post-inoculation in animals either infested with Boophilus microplus ticks (infected with B. bigemina), or inoculated with B. bigemina infected blood. Antibody titers decreased after day 33; however, all animals remained positive until the end of the experiment (124 days). The ELISA described may prove to be an appropriate serological test for the detection of IgM antibodies against B. bigemina.Universidade Federal de Minas Gerais Escola de VeterináriaUniversidade Federal de Minas Gerais Departamento de ParasitologiaUnesp Faculdade de Ciências Agrárias e VeterináriasUnesp Faculdade de Ciências Agrárias e VeterináriasInstituto Oswaldo Cruz, Ministério da SaúdeUniversidade Federal de Minas Gerais (UFMG)Universidade Estadual Paulista (Unesp)Ruiz, Patrícia M GonçalvesPassos, Lygia MFMachado, Rosangela Z [UNESP]Lima, José DRibeiro, Múcio FB2014-05-20T15:09:24Z2014-05-20T15:09:24Z2001-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article237-240application/pdfhttp://dx.doi.org/10.1590/S0074-02762001000200017Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 96, n. 2, p. 237-240, 2001.0074-0276http://hdl.handle.net/11449/2720710.1590/S0074-02762001000200017S0074-02762001000200017S0074-02762001000200017.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemórias do Instituto Oswaldo Cruz2.8331,172info:eu-repo/semantics/openAccess2024-06-07T13:02:58Zoai:repositorio.unesp.br:11449/27207Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:04:37.775548Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattle |
title |
Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattle |
spellingShingle |
Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattle Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattle Ruiz, Patrícia M Gonçalves Babesia bigemina ELISA IgM Ruiz, Patrícia M Gonçalves Babesia bigemina ELISA IgM |
title_short |
Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattle |
title_full |
Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattle |
title_fullStr |
Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattle Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattle |
title_full_unstemmed |
Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattle Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattle |
title_sort |
Development of an enzyme-linked immunosorbent assay for detection of IgM antibodies to Babesia bigemina in cattle |
author |
Ruiz, Patrícia M Gonçalves |
author_facet |
Ruiz, Patrícia M Gonçalves Ruiz, Patrícia M Gonçalves Passos, Lygia MF Machado, Rosangela Z [UNESP] Lima, José D Ribeiro, Múcio FB Passos, Lygia MF Machado, Rosangela Z [UNESP] Lima, José D Ribeiro, Múcio FB |
author_role |
author |
author2 |
Passos, Lygia MF Machado, Rosangela Z [UNESP] Lima, José D Ribeiro, Múcio FB |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Minas Gerais (UFMG) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ruiz, Patrícia M Gonçalves Passos, Lygia MF Machado, Rosangela Z [UNESP] Lima, José D Ribeiro, Múcio FB |
dc.subject.por.fl_str_mv |
Babesia bigemina ELISA IgM |
topic |
Babesia bigemina ELISA IgM |
description |
A crude antigenic preparation of Babesia bigemina was used to develop an ELISA for the detection of IgM antibodies. Optimal dilutions of the antigen, using positive and negative reference sera, were determined by checkerboard titrations. Negative sera from cattle imported from tick-free areas, serum samples collected from infected B. bigemina cattle were used to validate the test. The specificity was 94% and sensitivity of the Elisa 87.5%. Sera from 385 cattle deriving from areas free from tick-borne diseases, which were submitted to a preimmunization process, were screened by this technique. The Elisa detected seroconversion on the 14th day post-inoculation in animals either infested with Boophilus microplus ticks (infected with B. bigemina), or inoculated with B. bigemina infected blood. Antibody titers decreased after day 33; however, all animals remained positive until the end of the experiment (124 days). The ELISA described may prove to be an appropriate serological test for the detection of IgM antibodies against B. bigemina. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-02-01 2014-05-20T15:09:24Z 2014-05-20T15:09:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0074-02762001000200017 Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 96, n. 2, p. 237-240, 2001. 0074-0276 http://hdl.handle.net/11449/27207 10.1590/S0074-02762001000200017 S0074-02762001000200017 S0074-02762001000200017.pdf |
url |
http://dx.doi.org/10.1590/S0074-02762001000200017 http://hdl.handle.net/11449/27207 |
identifier_str_mv |
Memórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 96, n. 2, p. 237-240, 2001. 0074-0276 10.1590/S0074-02762001000200017 S0074-02762001000200017 S0074-02762001000200017.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz 2.833 1,172 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
237-240 application/pdf |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1822182589273735168 |
dc.identifier.doi.none.fl_str_mv |
10.1590/S0074-02762001000200017 |