Ocorrência de parasitoses gastrointestinais em cordeiros no município de Alambari, São Paulo
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/94677 |
Resumo: | The objective of this research was to determine the occurrence of gastrointestinal parasites and molecularly characterize the species of Cryptosporidium spp. in stool samples from lambs under one year old in the city of Alambari, São Paulo. A total of 193 samples were collected in 126 and the same was done counting eggs per gram of feces (EPG) Gordon and Whitlock (1939), to determine the presence of helminths and coccidia of the genus Eimeria. All samples were evaluated by nested-PCR amplification of DNA fragments subunit 18S rRNA gene for Cryptosporidium. By using the Chi- square and/or Fisher was significant association of the presence of intestinal parasites in relation to gender, race and age of the animals. Of the 126 samples analyzed, 71.4% were positive by OPG, identifying 58.9% of eggs of Strongyles, 5.6% of oocysts of Eimeria spp.; Coinfection in 14.4% of Strongyles and Moniezia spp., and 15.5% Strongyles and Eimeria spp., and 5.6% infection by the three parasites. Stool cultures were positive in 16 samples, with observation of genres: 31.3% Haemonchus spp., 12.5% Trichostrongylus spp.; Oesophagsotumum spp. in one, and 50% mixed infection with Haemonchus and Trichostrongylus. To Eimeria spp., the incidence was 19% of the animals, identifying the species: E. crandallis; E. faurei; E. marsica; E. ovinoidalis; E. pallida; E. parva and E. weybridgensis. The nPCR was positive in 15% and sequencing was performed in 19 samples with detection of species: C. xiaoi in 15 samples, C. ubiquituim three, and C. meleagridis in a sample, the latter being considered zoonotic species |
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Ocorrência de parasitoses gastrointestinais em cordeiros no município de Alambari, São PauloCryptosporidiumOvinoHelmintoEimeriaMolecular biologyThe objective of this research was to determine the occurrence of gastrointestinal parasites and molecularly characterize the species of Cryptosporidium spp. in stool samples from lambs under one year old in the city of Alambari, São Paulo. A total of 193 samples were collected in 126 and the same was done counting eggs per gram of feces (EPG) Gordon and Whitlock (1939), to determine the presence of helminths and coccidia of the genus Eimeria. All samples were evaluated by nested-PCR amplification of DNA fragments subunit 18S rRNA gene for Cryptosporidium. By using the Chi- square and/or Fisher was significant association of the presence of intestinal parasites in relation to gender, race and age of the animals. Of the 126 samples analyzed, 71.4% were positive by OPG, identifying 58.9% of eggs of Strongyles, 5.6% of oocysts of Eimeria spp.; Coinfection in 14.4% of Strongyles and Moniezia spp., and 15.5% Strongyles and Eimeria spp., and 5.6% infection by the three parasites. Stool cultures were positive in 16 samples, with observation of genres: 31.3% Haemonchus spp., 12.5% Trichostrongylus spp.; Oesophagsotumum spp. in one, and 50% mixed infection with Haemonchus and Trichostrongylus. To Eimeria spp., the incidence was 19% of the animals, identifying the species: E. crandallis; E. faurei; E. marsica; E. ovinoidalis; E. pallida; E. parva and E. weybridgensis. The nPCR was positive in 15% and sequencing was performed in 19 samples with detection of species: C. xiaoi in 15 samples, C. ubiquituim three, and C. meleagridis in a sample, the latter being considered zoonotic speciesFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAPESP: 10/52542-3Universidade Estadual Paulista (Unesp)Bresciani, Katia Denise Saraiva [UNESP]Universidade Estadual Paulista (Unesp)Zucatto, Anaíza Simão [UNESP]2014-06-11T19:27:17Z2014-06-11T19:27:17Z2013-04-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis56 f. : il.application/pdfZUCATTO, Anaíza Simão. Ocorrência de parasitoses gastrointestinais em cordeiros no município de Alambari, São Paulo. 2013. 56 f. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária de Araçatuba, 2013.http://hdl.handle.net/11449/94677000737488000737488.pdf33004021075P85950594366829647Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2023-12-07T06:20:23Zoai:repositorio.unesp.br:11449/94677Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-07T06:20:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Ocorrência de parasitoses gastrointestinais em cordeiros no município de Alambari, São Paulo |
title |
Ocorrência de parasitoses gastrointestinais em cordeiros no município de Alambari, São Paulo |
spellingShingle |
Ocorrência de parasitoses gastrointestinais em cordeiros no município de Alambari, São Paulo Zucatto, Anaíza Simão [UNESP] Cryptosporidium Ovino Helminto Eimeria Molecular biology |
title_short |
Ocorrência de parasitoses gastrointestinais em cordeiros no município de Alambari, São Paulo |
title_full |
Ocorrência de parasitoses gastrointestinais em cordeiros no município de Alambari, São Paulo |
title_fullStr |
Ocorrência de parasitoses gastrointestinais em cordeiros no município de Alambari, São Paulo |
title_full_unstemmed |
Ocorrência de parasitoses gastrointestinais em cordeiros no município de Alambari, São Paulo |
title_sort |
Ocorrência de parasitoses gastrointestinais em cordeiros no município de Alambari, São Paulo |
author |
Zucatto, Anaíza Simão [UNESP] |
author_facet |
Zucatto, Anaíza Simão [UNESP] |
author_role |
author |
dc.contributor.none.fl_str_mv |
Bresciani, Katia Denise Saraiva [UNESP] Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Zucatto, Anaíza Simão [UNESP] |
dc.subject.por.fl_str_mv |
Cryptosporidium Ovino Helminto Eimeria Molecular biology |
topic |
Cryptosporidium Ovino Helminto Eimeria Molecular biology |
description |
The objective of this research was to determine the occurrence of gastrointestinal parasites and molecularly characterize the species of Cryptosporidium spp. in stool samples from lambs under one year old in the city of Alambari, São Paulo. A total of 193 samples were collected in 126 and the same was done counting eggs per gram of feces (EPG) Gordon and Whitlock (1939), to determine the presence of helminths and coccidia of the genus Eimeria. All samples were evaluated by nested-PCR amplification of DNA fragments subunit 18S rRNA gene for Cryptosporidium. By using the Chi- square and/or Fisher was significant association of the presence of intestinal parasites in relation to gender, race and age of the animals. Of the 126 samples analyzed, 71.4% were positive by OPG, identifying 58.9% of eggs of Strongyles, 5.6% of oocysts of Eimeria spp.; Coinfection in 14.4% of Strongyles and Moniezia spp., and 15.5% Strongyles and Eimeria spp., and 5.6% infection by the three parasites. Stool cultures were positive in 16 samples, with observation of genres: 31.3% Haemonchus spp., 12.5% Trichostrongylus spp.; Oesophagsotumum spp. in one, and 50% mixed infection with Haemonchus and Trichostrongylus. To Eimeria spp., the incidence was 19% of the animals, identifying the species: E. crandallis; E. faurei; E. marsica; E. ovinoidalis; E. pallida; E. parva and E. weybridgensis. The nPCR was positive in 15% and sequencing was performed in 19 samples with detection of species: C. xiaoi in 15 samples, C. ubiquituim three, and C. meleagridis in a sample, the latter being considered zoonotic species |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-04-26 2014-06-11T19:27:17Z 2014-06-11T19:27:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
ZUCATTO, Anaíza Simão. Ocorrência de parasitoses gastrointestinais em cordeiros no município de Alambari, São Paulo. 2013. 56 f. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária de Araçatuba, 2013. http://hdl.handle.net/11449/94677 000737488 000737488.pdf 33004021075P8 5950594366829647 |
identifier_str_mv |
ZUCATTO, Anaíza Simão. Ocorrência de parasitoses gastrointestinais em cordeiros no município de Alambari, São Paulo. 2013. 56 f. Dissertação (mestrado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Medicina Veterinária de Araçatuba, 2013. 000737488 000737488.pdf 33004021075P8 5950594366829647 |
url |
http://hdl.handle.net/11449/94677 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
56 f. : il. application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.source.none.fl_str_mv |
Aleph reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046841407766528 |