Avaliação do processo de reparo alveolar em ratas com deficiência de estrógeno tratadas com OPG-Fc: análise imunoistoquímica, por microtomografia computadorizada e microscopia confocal

• Autor(a) principal: Silva, Ana Cláudia Ervolino da [UNESP]
• Data de Publicação: 2018
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/204008; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2019-05-29/000913991.pdf
• topic: Osteoporose; Denosumab; Regeneração óssea; Osteoporosis

The purpose of this study is to evaluate the bone repair formed after the upper incisor extraction of ovariectomized rats treated with OPG-Fc, simulating the action of Denosumab, drug used in anti-osteoporosis therapy. Thirty six (36) adult rats were divided into three experimental groups: 12 sham rats (SHAM), 12 ovariectomized rats (OVX/ST) and 12 ovariectomized rats treated with OPG-Fc (OVX/OPG-Fc). On day 0, ovariectomy surgery or fictional surgery was performed, after 30 days the treatment with OPG-Fc was started by subcutaneous injection (10mg/kg/2 times per week). After 30 days of medication, was performed the surgery of extraction of the upper right incisor. The euthanasia occurred 60 days after the extraction surgery, in wich the jaws containing the alveoli under repair was removed in order to perform microtomographic analysis (micro-CT), for the parameters of BV/TV, Tb.Th, Tb.N, Tb.Sp, and Po(Tot); immunohistochemical analysis directed to immunomarkers: OPG, RANKL, TRAP e Osteocalcin; and confocal microscopy evaluation, to determine the NBA (neoformed bone area) and MAR (mineral apposition rate). Quantitative data from micro-CT and confocal microscopy (NBA and MAR) were submited to homoscedasticity analysis (Shapiro-Wilk test) to distinguish parametric and nonparametric data. The parametric data was compared by ONE WAY ANOVA test and Tukey post-test, and the significance level was set at p<0.05. By immunohistochemical analysis, the OPG protein showed light to moderate labeling in the SHAM group and moderate in the OVX and OVX / OPG-Fc groups. For RANKL, moderate immunostaining was observed in the SHAM group, intense in the OVX group and mild in the OVX / OPG-Fc group. For OCN, moderate labeling was observed in all groups. The TRAP protein presented light marking in the SHAM and OVX / OPG-Fc groups...