Preparação de copolímeros PEG-PCL modificado com ácido fólico para liberação controlada de metotrexato

• Autor(a) principal: Brandt, João Victor [UNESP]
• Data de Publicação: 2016
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/155535; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-06-19/000885535.pdf
• topic: Tecnologia de liberação controlada; Copolímeros bloco; Micelas; Fármacos; Metotrexato; Block copolymers

Several drugs have low solubility within the human body, decreasing its bioavailability. Currently, several liberation platforms have been studied in order to overcome this limitation. Among the carriers employed, functionalized block copolymers have the advantage of interacting with the drug both hydrophilic and hydrophobically. Thus, the study of these platforms is fundamental for the encapsulation of hydrophobic drugs, allowing a greater time of circulation in the organism. The efficiency of incorporation and controlled release is dependent on the size, morphology and interactions of the copolymer blocks. The study focused on the physico-chemical properties of these materials (colloidal stability and micelle size, polymer chain length and micelle-drug affinity) in order to obtain better incorporation results. The use of these carriers is promising in the development of drug delivery systems. The reduction of the side effects, due to the use of these carriers, will allow the patient a less aggressive and more efficient treatment, improving the quality of life. In this work, the formation of micelles of PEG-co-PCL diblock copolymers with and without folic acid functionalization with varying chain size was evaluated and the efficiency of methotrexate incorporation was evaluated. The synthetic method, in which the monomers were added in the presence of catalyst in an inert atmosphere to a round bottom flask, allowed obtainingsamples with low polydispersity index. The samples were characterized by several techniques, such as: infrared transmittance spectroscopy, proton nuclear magnetic resonance spectroscopy, gel permeation chromatography, thermogravimetry, differential scanning calorimetry andX-ray diffraction. The micellar stability determined from the influence of the pH and temperature on the zeta potential and the hydrodynamic diameter of the micelles, in which the micelles were not influenced by temperature and added at pH values close to 4...