Analysis of polymorphisms in Interleukin 10, NOS2A, and ESR2 genes in chronic and aggressive periodontitis
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1807-3107BOR-2016.vol30.0105 http://hdl.handle.net/11449/176995 |
Resumo: | The objective of this study was to investigate the association between single nucleotide polymorphisms (SNPs) in the IL10, NOS2A, and ESR2 genes and chronic periodontitis (CP) and aggressive periodontitis (AgP). Three groups of patients underwent periodontal and radiographic evaluations: CP (n = 61), AgP (n = 50), and periodontally healthy (control group=61). Genomic DNA was extracted from oral epithelial cells and used for genotyping by real-time polymerase chain reaction using TaqMan® probes. The investigated SNPs were: -1087G > A, -819C > T and -592C > A in the IL10; +2087G > A in the NOS2A, and +1730G > A in the ESR2 gene. Differences in genotype and allele frequencies of each polymorphism and some individual characteristics were analyzed using the chi-square test and multivariate logistic regression analysis. Analysis of SNPs and haplotypes in the IL10 and SNP in the ESR2 gene did not present any significant association with AgP or CP. The +2087G allele of the NOS2A gene tended to be significantly associated with periodontal disease. Patients carrying the genotype +2087GG in the NOS2A gene were genetically protected against the development of CP (p = 0.05; OR = 0.44; 95%CI = 0.20-0.95). This result showed greater significance when patients with AgP and CP were combined (total PD) (p = 0.03; OR = 0.46; 95%CI = 0.23-0.92). In conclusion, the studied Brazilian population had a significantly higher frequency of the GG genotype for the +2087 SNP in the NOS2A gene in individuals without periodontitis, although statistical significance was not maintained after multiple logistic regression. |
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spelling |
Analysis of polymorphisms in Interleukin 10, NOS2A, and ESR2 genes in chronic and aggressive periodontitisThe objective of this study was to investigate the association between single nucleotide polymorphisms (SNPs) in the IL10, NOS2A, and ESR2 genes and chronic periodontitis (CP) and aggressive periodontitis (AgP). Three groups of patients underwent periodontal and radiographic evaluations: CP (n = 61), AgP (n = 50), and periodontally healthy (control group=61). Genomic DNA was extracted from oral epithelial cells and used for genotyping by real-time polymerase chain reaction using TaqMan® probes. The investigated SNPs were: -1087G > A, -819C > T and -592C > A in the IL10; +2087G > A in the NOS2A, and +1730G > A in the ESR2 gene. Differences in genotype and allele frequencies of each polymorphism and some individual characteristics were analyzed using the chi-square test and multivariate logistic regression analysis. Analysis of SNPs and haplotypes in the IL10 and SNP in the ESR2 gene did not present any significant association with AgP or CP. The +2087G allele of the NOS2A gene tended to be significantly associated with periodontal disease. Patients carrying the genotype +2087GG in the NOS2A gene were genetically protected against the development of CP (p = 0.05; OR = 0.44; 95%CI = 0.20-0.95). This result showed greater significance when patients with AgP and CP were combined (total PD) (p = 0.03; OR = 0.46; 95%CI = 0.23-0.92). In conclusion, the studied Brazilian population had a significantly higher frequency of the GG genotype for the +2087 SNP in the NOS2A gene in individuals without periodontitis, although statistical significance was not maintained after multiple logistic regression.Universidade Federal do Ceará - UFC, Campus Sobral, School of Dentistry, Department of Dentistry, Sobral, CE, BrazilUniversidade Estadual Paulista Júlio de Mesquita Filho - UNESP, School of Dentistry at Araraquara, Department of Morphology, Araraquara, SP, BrazilUniversidade Estadual Paulista Júlio de Mesquita Filho - UNESP, School of Dentistry at Araraquara, Department of Oral Diagnosis and Surgery, Araraquara, SP, BrazilUniversidade Federal do Ceará - UFC, Faculty of Pharmacy, Dentistry and Nursing, Department of Clinical and Toxicological Analyses, Fortaleza, CE, BrazilUniversidade Estadual Paulista (Unesp)Silveira, Virginia Régia SouzaPigossi, Suzane CristinaScarel-Caminaga, Raquel MantuaneliCirelli, Joni AugustoRêgo, RodrigoNogueira, Nádia Accioly Pinto2018-12-11T17:23:26Z2018-12-11T17:23:26Z2016-10-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlee105application/pdfhttp://dx.doi.org/10.1590/1807-3107BOR-2016.vol30.0105Brazilian oral research, v. 30, n. 1, p. e105-, 2016.1807-3107http://hdl.handle.net/11449/17699510.1590/1807-3107BOR-2016.vol30.0105S1806-832420160001002952-s2.0-85017316538S1806-83242016000100295.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian oral researchinfo:eu-repo/semantics/openAccess2024-01-18T06:30:52Zoai:repositorio.unesp.br:11449/176995Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:22:14.051970Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Analysis of polymorphisms in Interleukin 10, NOS2A, and ESR2 genes in chronic and aggressive periodontitis |
title |
Analysis of polymorphisms in Interleukin 10, NOS2A, and ESR2 genes in chronic and aggressive periodontitis |
spellingShingle |
Analysis of polymorphisms in Interleukin 10, NOS2A, and ESR2 genes in chronic and aggressive periodontitis Silveira, Virginia Régia Souza |
title_short |
Analysis of polymorphisms in Interleukin 10, NOS2A, and ESR2 genes in chronic and aggressive periodontitis |
title_full |
Analysis of polymorphisms in Interleukin 10, NOS2A, and ESR2 genes in chronic and aggressive periodontitis |
title_fullStr |
Analysis of polymorphisms in Interleukin 10, NOS2A, and ESR2 genes in chronic and aggressive periodontitis |
title_full_unstemmed |
Analysis of polymorphisms in Interleukin 10, NOS2A, and ESR2 genes in chronic and aggressive periodontitis |
title_sort |
Analysis of polymorphisms in Interleukin 10, NOS2A, and ESR2 genes in chronic and aggressive periodontitis |
author |
Silveira, Virginia Régia Souza |
author_facet |
Silveira, Virginia Régia Souza Pigossi, Suzane Cristina Scarel-Caminaga, Raquel Mantuaneli Cirelli, Joni Augusto Rêgo, Rodrigo Nogueira, Nádia Accioly Pinto |
author_role |
author |
author2 |
Pigossi, Suzane Cristina Scarel-Caminaga, Raquel Mantuaneli Cirelli, Joni Augusto Rêgo, Rodrigo Nogueira, Nádia Accioly Pinto |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Silveira, Virginia Régia Souza Pigossi, Suzane Cristina Scarel-Caminaga, Raquel Mantuaneli Cirelli, Joni Augusto Rêgo, Rodrigo Nogueira, Nádia Accioly Pinto |
description |
The objective of this study was to investigate the association between single nucleotide polymorphisms (SNPs) in the IL10, NOS2A, and ESR2 genes and chronic periodontitis (CP) and aggressive periodontitis (AgP). Three groups of patients underwent periodontal and radiographic evaluations: CP (n = 61), AgP (n = 50), and periodontally healthy (control group=61). Genomic DNA was extracted from oral epithelial cells and used for genotyping by real-time polymerase chain reaction using TaqMan® probes. The investigated SNPs were: -1087G > A, -819C > T and -592C > A in the IL10; +2087G > A in the NOS2A, and +1730G > A in the ESR2 gene. Differences in genotype and allele frequencies of each polymorphism and some individual characteristics were analyzed using the chi-square test and multivariate logistic regression analysis. Analysis of SNPs and haplotypes in the IL10 and SNP in the ESR2 gene did not present any significant association with AgP or CP. The +2087G allele of the NOS2A gene tended to be significantly associated with periodontal disease. Patients carrying the genotype +2087GG in the NOS2A gene were genetically protected against the development of CP (p = 0.05; OR = 0.44; 95%CI = 0.20-0.95). This result showed greater significance when patients with AgP and CP were combined (total PD) (p = 0.03; OR = 0.46; 95%CI = 0.23-0.92). In conclusion, the studied Brazilian population had a significantly higher frequency of the GG genotype for the +2087 SNP in the NOS2A gene in individuals without periodontitis, although statistical significance was not maintained after multiple logistic regression. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-10-10 2018-12-11T17:23:26Z 2018-12-11T17:23:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1807-3107BOR-2016.vol30.0105 Brazilian oral research, v. 30, n. 1, p. e105-, 2016. 1807-3107 http://hdl.handle.net/11449/176995 10.1590/1807-3107BOR-2016.vol30.0105 S1806-83242016000100295 2-s2.0-85017316538 S1806-83242016000100295.pdf |
url |
http://dx.doi.org/10.1590/1807-3107BOR-2016.vol30.0105 http://hdl.handle.net/11449/176995 |
identifier_str_mv |
Brazilian oral research, v. 30, n. 1, p. e105-, 2016. 1807-3107 10.1590/1807-3107BOR-2016.vol30.0105 S1806-83242016000100295 2-s2.0-85017316538 S1806-83242016000100295.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian oral research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
e105 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129512142012416 |