Amplitude-modulated cold atmospheric pressure plasma jet for treatment of oral candidiasis: In vivo study
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0199832 http://hdl.handle.net/11449/176515 |
Resumo: | The aim of this study was to establish an effective and safe protocol for in vivo oral candidiasis treatment with atmospheric plasma jets. A novel amplitude-modulated cold atmospheric pressure plasma jet (AM-CAPPJ) device, operating with Helium, was tested. In vitro assays with Candida albicans biofilms and Vero cells were performed in order to determine the effective parameters with low cytotoxicity. After the determination of such parameters, the protocol was evaluated in experimentally induced oral candidiasis in mice. AM-CAPPJ could significantly reduce the viability of C. albicans biofilms after 5 minutes of plasma exposure when compared to the non-exposed group (p = 0.0033). After this period of exposure, high viability of Vero cells was maintained (86.33 ± 10.45%). Also, no late effects on these cells were observed after 24 and 48 hours (83.24±15.23% and 88.96±18.65%, respectively). Histological analyses revealed significantly lower occurrence of inflammatory alterations in the AM-CAPPJ group when compared to non-treated and nystatin-treated groups (p < 0.0001). Although no significant differences among the values of CFU/tongue were observed among the non-treated group and the groups treated with AM-CAPPJ or nystatin (p = 0.3201), histological analyses revealed marked reduction in candidal tissue invasion. In conclusion, these results point out to a clinical applicability of this protocol, due to the simultaneous anti-inflammatory and inhibitory effects of AM-CAPPJ with low cytotoxicity. |
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Amplitude-modulated cold atmospheric pressure plasma jet for treatment of oral candidiasis: In vivo studyThe aim of this study was to establish an effective and safe protocol for in vivo oral candidiasis treatment with atmospheric plasma jets. A novel amplitude-modulated cold atmospheric pressure plasma jet (AM-CAPPJ) device, operating with Helium, was tested. In vitro assays with Candida albicans biofilms and Vero cells were performed in order to determine the effective parameters with low cytotoxicity. After the determination of such parameters, the protocol was evaluated in experimentally induced oral candidiasis in mice. AM-CAPPJ could significantly reduce the viability of C. albicans biofilms after 5 minutes of plasma exposure when compared to the non-exposed group (p = 0.0033). After this period of exposure, high viability of Vero cells was maintained (86.33 ± 10.45%). Also, no late effects on these cells were observed after 24 and 48 hours (83.24±15.23% and 88.96±18.65%, respectively). Histological analyses revealed significantly lower occurrence of inflammatory alterations in the AM-CAPPJ group when compared to non-treated and nystatin-treated groups (p < 0.0001). Although no significant differences among the values of CFU/tongue were observed among the non-treated group and the groups treated with AM-CAPPJ or nystatin (p = 0.3201), histological analyses revealed marked reduction in candidal tissue invasion. In conclusion, these results point out to a clinical applicability of this protocol, due to the simultaneous anti-inflammatory and inhibitory effects of AM-CAPPJ with low cytotoxicity.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Environmental Engineering and Oral Biopathology Graduate Program Institute of Science and Technology São Paulo State University (UNESP)Department of Chemistry and Physics Guaratinguetá Faculty of Engineering São Paulo State University (UNESP)Department of Environmental Engineering and Oral Biopathology Graduate Program Institute of Science and Technology São Paulo State University (UNESP)Department of Chemistry and Physics Guaratinguetá Faculty of Engineering São Paulo State University (UNESP)FAPESP: #2014/02354-7FAPESP: #2015/ 21989-6FAPESP: #2015/21989-6FAPESP: #2016/07196-6Universidade Estadual Paulista (Unesp)Borges, Aline Chiodi [UNESP]de Morais Gouvêa Lima, Gabriela [UNESP]Mayumi Castaldelli Nishime, Thalita [UNESP]Vidal Lacerda Gontijo, Aline [UNESP]Kostov, Konstantin Georgiev [UNESP]Koga-Ito, Cristiane Yumi [UNESP]2018-12-11T17:21:10Z2018-12-11T17:21:10Z2018-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1371/journal.pone.0199832PLoS ONE, v. 13, n. 6, 2018.1932-6203http://hdl.handle.net/11449/17651510.1371/journal.pone.01998322-s2.0-850491526442-s2.0-85049152644.pdf194650980100045065435631614034210000-0002-2416-2173Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLoS ONE1,164info:eu-repo/semantics/openAccess2024-07-01T20:52:47Zoai:repositorio.unesp.br:11449/176515Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:22:11.990080Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Amplitude-modulated cold atmospheric pressure plasma jet for treatment of oral candidiasis: In vivo study |
title |
Amplitude-modulated cold atmospheric pressure plasma jet for treatment of oral candidiasis: In vivo study |
spellingShingle |
Amplitude-modulated cold atmospheric pressure plasma jet for treatment of oral candidiasis: In vivo study Borges, Aline Chiodi [UNESP] |
title_short |
Amplitude-modulated cold atmospheric pressure plasma jet for treatment of oral candidiasis: In vivo study |
title_full |
Amplitude-modulated cold atmospheric pressure plasma jet for treatment of oral candidiasis: In vivo study |
title_fullStr |
Amplitude-modulated cold atmospheric pressure plasma jet for treatment of oral candidiasis: In vivo study |
title_full_unstemmed |
Amplitude-modulated cold atmospheric pressure plasma jet for treatment of oral candidiasis: In vivo study |
title_sort |
Amplitude-modulated cold atmospheric pressure plasma jet for treatment of oral candidiasis: In vivo study |
author |
Borges, Aline Chiodi [UNESP] |
author_facet |
Borges, Aline Chiodi [UNESP] de Morais Gouvêa Lima, Gabriela [UNESP] Mayumi Castaldelli Nishime, Thalita [UNESP] Vidal Lacerda Gontijo, Aline [UNESP] Kostov, Konstantin Georgiev [UNESP] Koga-Ito, Cristiane Yumi [UNESP] |
author_role |
author |
author2 |
de Morais Gouvêa Lima, Gabriela [UNESP] Mayumi Castaldelli Nishime, Thalita [UNESP] Vidal Lacerda Gontijo, Aline [UNESP] Kostov, Konstantin Georgiev [UNESP] Koga-Ito, Cristiane Yumi [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Borges, Aline Chiodi [UNESP] de Morais Gouvêa Lima, Gabriela [UNESP] Mayumi Castaldelli Nishime, Thalita [UNESP] Vidal Lacerda Gontijo, Aline [UNESP] Kostov, Konstantin Georgiev [UNESP] Koga-Ito, Cristiane Yumi [UNESP] |
description |
The aim of this study was to establish an effective and safe protocol for in vivo oral candidiasis treatment with atmospheric plasma jets. A novel amplitude-modulated cold atmospheric pressure plasma jet (AM-CAPPJ) device, operating with Helium, was tested. In vitro assays with Candida albicans biofilms and Vero cells were performed in order to determine the effective parameters with low cytotoxicity. After the determination of such parameters, the protocol was evaluated in experimentally induced oral candidiasis in mice. AM-CAPPJ could significantly reduce the viability of C. albicans biofilms after 5 minutes of plasma exposure when compared to the non-exposed group (p = 0.0033). After this period of exposure, high viability of Vero cells was maintained (86.33 ± 10.45%). Also, no late effects on these cells were observed after 24 and 48 hours (83.24±15.23% and 88.96±18.65%, respectively). Histological analyses revealed significantly lower occurrence of inflammatory alterations in the AM-CAPPJ group when compared to non-treated and nystatin-treated groups (p < 0.0001). Although no significant differences among the values of CFU/tongue were observed among the non-treated group and the groups treated with AM-CAPPJ or nystatin (p = 0.3201), histological analyses revealed marked reduction in candidal tissue invasion. In conclusion, these results point out to a clinical applicability of this protocol, due to the simultaneous anti-inflammatory and inhibitory effects of AM-CAPPJ with low cytotoxicity. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:21:10Z 2018-12-11T17:21:10Z 2018-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0199832 PLoS ONE, v. 13, n. 6, 2018. 1932-6203 http://hdl.handle.net/11449/176515 10.1371/journal.pone.0199832 2-s2.0-85049152644 2-s2.0-85049152644.pdf 1946509801000450 6543563161403421 0000-0002-2416-2173 |
url |
http://dx.doi.org/10.1371/journal.pone.0199832 http://hdl.handle.net/11449/176515 |
identifier_str_mv |
PLoS ONE, v. 13, n. 6, 2018. 1932-6203 10.1371/journal.pone.0199832 2-s2.0-85049152644 2-s2.0-85049152644.pdf 1946509801000450 6543563161403421 0000-0002-2416-2173 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLoS ONE 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129512075952128 |