Performance of commercial mycoplasma hyopneumoniae serum enzyme-linked immunosorbent assays under experimental and field conditions
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1128/JCM.00485-20 http://hdl.handle.net/11449/221611 |
Resumo: | Mycoplasma hyopneumoniae is an economically significant pathogen of swine. M. hyopneumoniae serum antibody detection via commercial enzyme-linked immunosorbent assays (ELISAs) is widely used for routine surveillance in commercial swine production systems. Samples from two studies were used to evaluate assay performance. In study 1, 6 commercial M. hyopneumoniae ELISAs were compared using serum samples from 8-week-old cesarean-derived, colostrum-deprived (CDCD) pigs allocated to the following 5 inoculation groups of 10 pigs each: (i) negative control, (ii) Mycoplasma flocculare (strain 27399), (iii) Mycoplasma hyorhinis (strain 38983), (iv) Mycoplasma hyosynoviae (strain 34428), and (v) M. hyopneumoniae (strain 232). Weekly serum and daily oral fluid samples were collected through 56 days postinoculation (dpi). The true status of pigs was established by PCR testing on oral fluids samples over the course of the observation period. Analysis of ELISA performance at various cutoffs found that the manufacturers' recommended cutoffs were diagnostically specific, i.e., produced no false positives, with the exceptions of 2 ELISAs. An analysis based on overall misclassification error rates found that 4 ELISAs performed similarly, although one assay produced more false positives. In study 2, the 3 best-performing ELISAs from study 1 were compared using serum samples generated under field conditions. Ten 8-week-old pigs were intratracheally inoculated with M. hyopneumoniae. Matched serum and tracheal samples (to establish the true pig M. hyopneumoniae status) were collected at 7- to 14-day intervals through 98 dpi. Analyses of sensitivity and specificity showed similar performance among these 3 ELISAs. Overall, this study provides an assessment of the performance of current M. hyopneumoniae ELISAs and an understanding of their use in surveillance. |
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Performance of commercial mycoplasma hyopneumoniae serum enzyme-linked immunosorbent assays under experimental and field conditionsCross-reactivityELISAMisclassification error rateMycoplasma flocculareMycoplasma hyopneumoniaeMycoplasma hyorhinisMycoplasma hyosynoviaeOral fluidsTest performanceMycoplasma hyopneumoniae is an economically significant pathogen of swine. M. hyopneumoniae serum antibody detection via commercial enzyme-linked immunosorbent assays (ELISAs) is widely used for routine surveillance in commercial swine production systems. Samples from two studies were used to evaluate assay performance. In study 1, 6 commercial M. hyopneumoniae ELISAs were compared using serum samples from 8-week-old cesarean-derived, colostrum-deprived (CDCD) pigs allocated to the following 5 inoculation groups of 10 pigs each: (i) negative control, (ii) Mycoplasma flocculare (strain 27399), (iii) Mycoplasma hyorhinis (strain 38983), (iv) Mycoplasma hyosynoviae (strain 34428), and (v) M. hyopneumoniae (strain 232). Weekly serum and daily oral fluid samples were collected through 56 days postinoculation (dpi). The true status of pigs was established by PCR testing on oral fluids samples over the course of the observation period. Analysis of ELISA performance at various cutoffs found that the manufacturers' recommended cutoffs were diagnostically specific, i.e., produced no false positives, with the exceptions of 2 ELISAs. An analysis based on overall misclassification error rates found that 4 ELISAs performed similarly, although one assay produced more false positives. In study 2, the 3 best-performing ELISAs from study 1 were compared using serum samples generated under field conditions. Ten 8-week-old pigs were intratracheally inoculated with M. hyopneumoniae. Matched serum and tracheal samples (to establish the true pig M. hyopneumoniae status) were collected at 7- to 14-day intervals through 98 dpi. Analyses of sensitivity and specificity showed similar performance among these 3 ELISAs. Overall, this study provides an assessment of the performance of current M. hyopneumoniae ELISAs and an understanding of their use in surveillance.Department of Veterinary Diagnostic and Production Animal Medicine Iowa State UniversityUniversidade Estadual de São PauloPampanga State Agricultural UniversityPIC North AmericaUniversidade Estadual de São PauloIowa State UniversityUniversidade Estadual Paulista (UNESP)Pampanga State Agricultural UniversityPIC North AmericaSilva, Ana Paula S. PoetaMagtoto, Ronaldo L.Almeida, Henrique M. Souza [UNESP]McDaniel, AricMagtoto, Precy D.Derscheid, Rachel J.Merodio, Maria M.Ferreyra, Franco S. MatiasGatto, Igor R.H. [UNESP]Baum, David H.Clavijo, Maria J.Arruda, Bailey L.Zimmerman, Jeffrey J.Giménez-Lirola, Luis G.2022-04-28T19:29:46Z2022-04-28T19:29:46Z2020-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1128/JCM.00485-20Journal of Clinical Microbiology, v. 58, n. 12, 2020.1098-660X0095-1137http://hdl.handle.net/11449/22161110.1128/JCM.00485-202-s2.0-85096456983Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Clinical Microbiologyinfo:eu-repo/semantics/openAccess2022-04-28T19:29:46Zoai:repositorio.unesp.br:11449/221611Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:42:31.893518Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Performance of commercial mycoplasma hyopneumoniae serum enzyme-linked immunosorbent assays under experimental and field conditions |
title |
Performance of commercial mycoplasma hyopneumoniae serum enzyme-linked immunosorbent assays under experimental and field conditions |
spellingShingle |
Performance of commercial mycoplasma hyopneumoniae serum enzyme-linked immunosorbent assays under experimental and field conditions Silva, Ana Paula S. Poeta Cross-reactivity ELISA Misclassification error rate Mycoplasma flocculare Mycoplasma hyopneumoniae Mycoplasma hyorhinis Mycoplasma hyosynoviae Oral fluids Test performance |
title_short |
Performance of commercial mycoplasma hyopneumoniae serum enzyme-linked immunosorbent assays under experimental and field conditions |
title_full |
Performance of commercial mycoplasma hyopneumoniae serum enzyme-linked immunosorbent assays under experimental and field conditions |
title_fullStr |
Performance of commercial mycoplasma hyopneumoniae serum enzyme-linked immunosorbent assays under experimental and field conditions |
title_full_unstemmed |
Performance of commercial mycoplasma hyopneumoniae serum enzyme-linked immunosorbent assays under experimental and field conditions |
title_sort |
Performance of commercial mycoplasma hyopneumoniae serum enzyme-linked immunosorbent assays under experimental and field conditions |
author |
Silva, Ana Paula S. Poeta |
author_facet |
Silva, Ana Paula S. Poeta Magtoto, Ronaldo L. Almeida, Henrique M. Souza [UNESP] McDaniel, Aric Magtoto, Precy D. Derscheid, Rachel J. Merodio, Maria M. Ferreyra, Franco S. Matias Gatto, Igor R.H. [UNESP] Baum, David H. Clavijo, Maria J. Arruda, Bailey L. Zimmerman, Jeffrey J. Giménez-Lirola, Luis G. |
author_role |
author |
author2 |
Magtoto, Ronaldo L. Almeida, Henrique M. Souza [UNESP] McDaniel, Aric Magtoto, Precy D. Derscheid, Rachel J. Merodio, Maria M. Ferreyra, Franco S. Matias Gatto, Igor R.H. [UNESP] Baum, David H. Clavijo, Maria J. Arruda, Bailey L. Zimmerman, Jeffrey J. Giménez-Lirola, Luis G. |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Iowa State University Universidade Estadual Paulista (UNESP) Pampanga State Agricultural University PIC North America |
dc.contributor.author.fl_str_mv |
Silva, Ana Paula S. Poeta Magtoto, Ronaldo L. Almeida, Henrique M. Souza [UNESP] McDaniel, Aric Magtoto, Precy D. Derscheid, Rachel J. Merodio, Maria M. Ferreyra, Franco S. Matias Gatto, Igor R.H. [UNESP] Baum, David H. Clavijo, Maria J. Arruda, Bailey L. Zimmerman, Jeffrey J. Giménez-Lirola, Luis G. |
dc.subject.por.fl_str_mv |
Cross-reactivity ELISA Misclassification error rate Mycoplasma flocculare Mycoplasma hyopneumoniae Mycoplasma hyorhinis Mycoplasma hyosynoviae Oral fluids Test performance |
topic |
Cross-reactivity ELISA Misclassification error rate Mycoplasma flocculare Mycoplasma hyopneumoniae Mycoplasma hyorhinis Mycoplasma hyosynoviae Oral fluids Test performance |
description |
Mycoplasma hyopneumoniae is an economically significant pathogen of swine. M. hyopneumoniae serum antibody detection via commercial enzyme-linked immunosorbent assays (ELISAs) is widely used for routine surveillance in commercial swine production systems. Samples from two studies were used to evaluate assay performance. In study 1, 6 commercial M. hyopneumoniae ELISAs were compared using serum samples from 8-week-old cesarean-derived, colostrum-deprived (CDCD) pigs allocated to the following 5 inoculation groups of 10 pigs each: (i) negative control, (ii) Mycoplasma flocculare (strain 27399), (iii) Mycoplasma hyorhinis (strain 38983), (iv) Mycoplasma hyosynoviae (strain 34428), and (v) M. hyopneumoniae (strain 232). Weekly serum and daily oral fluid samples were collected through 56 days postinoculation (dpi). The true status of pigs was established by PCR testing on oral fluids samples over the course of the observation period. Analysis of ELISA performance at various cutoffs found that the manufacturers' recommended cutoffs were diagnostically specific, i.e., produced no false positives, with the exceptions of 2 ELISAs. An analysis based on overall misclassification error rates found that 4 ELISAs performed similarly, although one assay produced more false positives. In study 2, the 3 best-performing ELISAs from study 1 were compared using serum samples generated under field conditions. Ten 8-week-old pigs were intratracheally inoculated with M. hyopneumoniae. Matched serum and tracheal samples (to establish the true pig M. hyopneumoniae status) were collected at 7- to 14-day intervals through 98 dpi. Analyses of sensitivity and specificity showed similar performance among these 3 ELISAs. Overall, this study provides an assessment of the performance of current M. hyopneumoniae ELISAs and an understanding of their use in surveillance. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-01 2022-04-28T19:29:46Z 2022-04-28T19:29:46Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1128/JCM.00485-20 Journal of Clinical Microbiology, v. 58, n. 12, 2020. 1098-660X 0095-1137 http://hdl.handle.net/11449/221611 10.1128/JCM.00485-20 2-s2.0-85096456983 |
url |
http://dx.doi.org/10.1128/JCM.00485-20 http://hdl.handle.net/11449/221611 |
identifier_str_mv |
Journal of Clinical Microbiology, v. 58, n. 12, 2020. 1098-660X 0095-1137 10.1128/JCM.00485-20 2-s2.0-85096456983 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Clinical Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129108652064768 |