Use of the amphotericin B, miconazole, and sodium hypochlorite to control the growth of the robust Aspergillus flavus and Aspergillus fumigatus biofilms on polyethylene support
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.7324/JABB.2022.100404 http://hdl.handle.net/11449/241204 |
Resumo: | Aspergillus flavus and Aspergillus fumigatus are able to grow on surfaces, including medical equipment, forming robust and resistant biofilms, and protecting the fungal cells against antifungal agents. Based on that, A. flavus and A. fumigatus biofilms were treated for 48 h with amphotericin B, miconazole, and sodium hypochlorite. Amphotericin B and miconazole acted as fungistatic for both fungal strains. The minimal inhibitory concentration for the amphotericin B was 400 µg mL-1 and >833 µg mL-1 for A. flavus and A. fumigatus, respectively, for the miconazole was 600 µg mL-1 and 1200 µg mL-1. Sodium hypochlorite presented fungicide activity at 8.3 µg mL-1 on A. flavus biofilms, while for A. fumigatus biofilm only fungistatic activity was observed. A. flavus biofilms were most susceptive to the treatment with the antifungal agents than were A. fumigatus. Our observation suggests that these robust structures as an interesting model to study fungal resistance. |
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Use of the amphotericin B, miconazole, and sodium hypochlorite to control the growth of the robust Aspergillus flavus and Aspergillus fumigatus biofilms on polyethylene supportAntifungal treatmentAspergillus flavusAspergillus fumigatusFungal biofilmsFungal resistanceSodium hypochloriteAspergillus flavus and Aspergillus fumigatus are able to grow on surfaces, including medical equipment, forming robust and resistant biofilms, and protecting the fungal cells against antifungal agents. Based on that, A. flavus and A. fumigatus biofilms were treated for 48 h with amphotericin B, miconazole, and sodium hypochlorite. Amphotericin B and miconazole acted as fungistatic for both fungal strains. The minimal inhibitory concentration for the amphotericin B was 400 µg mL-1 and >833 µg mL-1 for A. flavus and A. fumigatus, respectively, for the miconazole was 600 µg mL-1 and 1200 µg mL-1. Sodium hypochlorite presented fungicide activity at 8.3 µg mL-1 on A. flavus biofilms, while for A. fumigatus biofilm only fungistatic activity was observed. A. flavus biofilms were most susceptive to the treatment with the antifungal agents than were A. fumigatus. Our observation suggests that these robust structures as an interesting model to study fungal resistance.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Biochemistry Instituto de Química de Araraquara Universidade Estadual Paulista “Júlio de Mesquita Filho”, São PauloDepartment of Clinical Analysis Toxicology and Bromatology Faculdade de Ciências Farmacêuticas de Ribeirão Preto USP, São PauloDepartment of Biology Faculdade de Filosofia Ciências e Letras de Ribeirão Preto USP, São PauloDepartment of Biochemistry Instituto de Química de Araraquara Universidade Estadual Paulista “Júlio de Mesquita Filho”, São PauloUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Francisco, Camila Guedes [UNESP]Braga, Gilberto Bida LeiteGuimarães, Luis Henrique Souza2023-03-01T20:51:41Z2023-03-01T20:51:41Z2022-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article27-34http://dx.doi.org/10.7324/JABB.2022.100404Journal of Applied Biology and Biotechnology, v. 10, n. 4, p. 27-34, 2022.2347-212Xhttp://hdl.handle.net/11449/24120410.7324/JABB.2022.1004042-s2.0-85132556796Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Biology and Biotechnologyinfo:eu-repo/semantics/openAccess2023-03-01T20:51:41Zoai:repositorio.unesp.br:11449/241204Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:21:44.256636Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Use of the amphotericin B, miconazole, and sodium hypochlorite to control the growth of the robust Aspergillus flavus and Aspergillus fumigatus biofilms on polyethylene support |
title |
Use of the amphotericin B, miconazole, and sodium hypochlorite to control the growth of the robust Aspergillus flavus and Aspergillus fumigatus biofilms on polyethylene support |
spellingShingle |
Use of the amphotericin B, miconazole, and sodium hypochlorite to control the growth of the robust Aspergillus flavus and Aspergillus fumigatus biofilms on polyethylene support Francisco, Camila Guedes [UNESP] Antifungal treatment Aspergillus flavus Aspergillus fumigatus Fungal biofilms Fungal resistance Sodium hypochlorite |
title_short |
Use of the amphotericin B, miconazole, and sodium hypochlorite to control the growth of the robust Aspergillus flavus and Aspergillus fumigatus biofilms on polyethylene support |
title_full |
Use of the amphotericin B, miconazole, and sodium hypochlorite to control the growth of the robust Aspergillus flavus and Aspergillus fumigatus biofilms on polyethylene support |
title_fullStr |
Use of the amphotericin B, miconazole, and sodium hypochlorite to control the growth of the robust Aspergillus flavus and Aspergillus fumigatus biofilms on polyethylene support |
title_full_unstemmed |
Use of the amphotericin B, miconazole, and sodium hypochlorite to control the growth of the robust Aspergillus flavus and Aspergillus fumigatus biofilms on polyethylene support |
title_sort |
Use of the amphotericin B, miconazole, and sodium hypochlorite to control the growth of the robust Aspergillus flavus and Aspergillus fumigatus biofilms on polyethylene support |
author |
Francisco, Camila Guedes [UNESP] |
author_facet |
Francisco, Camila Guedes [UNESP] Braga, Gilberto Bida Leite Guimarães, Luis Henrique Souza |
author_role |
author |
author2 |
Braga, Gilberto Bida Leite Guimarães, Luis Henrique Souza |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Francisco, Camila Guedes [UNESP] Braga, Gilberto Bida Leite Guimarães, Luis Henrique Souza |
dc.subject.por.fl_str_mv |
Antifungal treatment Aspergillus flavus Aspergillus fumigatus Fungal biofilms Fungal resistance Sodium hypochlorite |
topic |
Antifungal treatment Aspergillus flavus Aspergillus fumigatus Fungal biofilms Fungal resistance Sodium hypochlorite |
description |
Aspergillus flavus and Aspergillus fumigatus are able to grow on surfaces, including medical equipment, forming robust and resistant biofilms, and protecting the fungal cells against antifungal agents. Based on that, A. flavus and A. fumigatus biofilms were treated for 48 h with amphotericin B, miconazole, and sodium hypochlorite. Amphotericin B and miconazole acted as fungistatic for both fungal strains. The minimal inhibitory concentration for the amphotericin B was 400 µg mL-1 and >833 µg mL-1 for A. flavus and A. fumigatus, respectively, for the miconazole was 600 µg mL-1 and 1200 µg mL-1. Sodium hypochlorite presented fungicide activity at 8.3 µg mL-1 on A. flavus biofilms, while for A. fumigatus biofilm only fungistatic activity was observed. A. flavus biofilms were most susceptive to the treatment with the antifungal agents than were A. fumigatus. Our observation suggests that these robust structures as an interesting model to study fungal resistance. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-07-01 2023-03-01T20:51:41Z 2023-03-01T20:51:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.7324/JABB.2022.100404 Journal of Applied Biology and Biotechnology, v. 10, n. 4, p. 27-34, 2022. 2347-212X http://hdl.handle.net/11449/241204 10.7324/JABB.2022.100404 2-s2.0-85132556796 |
url |
http://dx.doi.org/10.7324/JABB.2022.100404 http://hdl.handle.net/11449/241204 |
identifier_str_mv |
Journal of Applied Biology and Biotechnology, v. 10, n. 4, p. 27-34, 2022. 2347-212X 10.7324/JABB.2022.100404 2-s2.0-85132556796 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Applied Biology and Biotechnology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
27-34 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129420348620800 |