Phospho-mTOR in non-tumour and tumour bladder urothelium: Pattern of expression and impact on urothelial bladder cancer patients
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.spandidos-publications.com/10.3892/ol.2014.2392 http://hdl.handle.net/11449/130292 |
Resumo: | Urothelial bladder carcinoma (UBC) is heterogeneous in its pathology and clinical behaviour. Evaluation of prognostic and predictive biomarkers is necessary, in order to produce personalised treatment options. The present study used immunohistochemistry to evaluate UBC sections containing tumour and non-tumour areas from 76 patients, for the detection of p-mTOR, CD31 and D2-40 (blood and lymphatic vessels identification, respectively). Of the non-tumour and tumour sections, 36 and 20% were scored positive for p-mTOR expression, respectively. Immunoexpression was observed in umbrella cells from non-tumour urothelium, in all cell layers from non-muscle-invasive (NMI) tumours (including expression in superficial cells), and in spots of cells from muscle-invasive (MI) tumours. Positive expression decreased from non-tumour to tumour urothelium, and from pTl/pTis to pT3/pT4 tumours; however, the few pT3/pT4 positive cases had worse survival rates, with 5-year disease-free survival being significantly lower. Angiogenesis occurrence was impaired in pT3/pT4 tumours that did not express p-mTOR. In conclusion, p-mTOR expression in non-tumour umbrella cells is likely a reflection of their metabolic plasticity, and extension to the inner layers of the urothelium in NMI tumours is consistent with an enhanced malignant potential. The expression in cell spots in a few MI tumours and absence of expression in the remaining tumours is intriguing and requires further research. Additional studies regarding the up- and downstream effectors of the mTOR pathway should be conducted. |
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Phospho-mTOR in non-tumour and tumour bladder urothelium: Pattern of expression and impact on urothelial bladder cancer patientsp-mTORurothelial bladder cancerpattern of expressionumbrella cellsUrothelial bladder carcinoma (UBC) is heterogeneous in its pathology and clinical behaviour. Evaluation of prognostic and predictive biomarkers is necessary, in order to produce personalised treatment options. The present study used immunohistochemistry to evaluate UBC sections containing tumour and non-tumour areas from 76 patients, for the detection of p-mTOR, CD31 and D2-40 (blood and lymphatic vessels identification, respectively). Of the non-tumour and tumour sections, 36 and 20% were scored positive for p-mTOR expression, respectively. Immunoexpression was observed in umbrella cells from non-tumour urothelium, in all cell layers from non-muscle-invasive (NMI) tumours (including expression in superficial cells), and in spots of cells from muscle-invasive (MI) tumours. Positive expression decreased from non-tumour to tumour urothelium, and from pTl/pTis to pT3/pT4 tumours; however, the few pT3/pT4 positive cases had worse survival rates, with 5-year disease-free survival being significantly lower. Angiogenesis occurrence was impaired in pT3/pT4 tumours that did not express p-mTOR. In conclusion, p-mTOR expression in non-tumour umbrella cells is likely a reflection of their metabolic plasticity, and extension to the inner layers of the urothelium in NMI tumours is consistent with an enhanced malignant potential. The expression in cell spots in a few MI tumours and absence of expression in the remaining tumours is intriguing and requires further research. Additional studies regarding the up- and downstream effectors of the mTOR pathway should be conducted.Univ Minho, Sch Healh Sci ECS, Life &Hlth Sci Res Inst ICVS, P-4710057 Braga, PortugalICVS 3Bs, PT Govt Associate Lab, P-4806909 Guimaraes, PortugalSao Paulo State Univ, Fac Med, LIM 14, BR-01246000 Sao Paulo, BrazilBarretos Canc Hosp, Mol Oncol Res Ctr, BR-14784400 Sao Paulo, BrazilIPO, Dept Urol, P-4200072 Oporto, PortugalIPO, Expt Pathol &Therapeut Res Ctr, P-4200072 Oporto, PortugalIPO, Dept Surg Oncol, P-4200072 Oporto, PortugalUniv Fernando Pessoa, Fac Hlth Sci, P-4200150 Oporto, PortugalSao Paulo State Univ, Fac Med, LIM 14, BR-01246000 Sao Paulo, BrazilSpandidos Publ LtdUniv MinhoICVS 3BsUniversidade Estadual Paulista (Unesp)Barretos Canc HospIPOUniv Fernando PessoaAfonso, JulietaLongatto-Filho, Adhemar [UNESP]Da Silva, Vitor MoreiraAmaro, TeresinaSantos, Iucio L.2015-11-03T18:06:25Z2015-11-03T18:06:25Z2014-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1447-1454application/pdfhttp://www.spandidos-publications.com/10.3892/ol.2014.2392Oncology Letters. Athens: Spandidos Publ Ltd, v. 8, n. 4, p. 1447-1454, 2014.1792-1074http://hdl.handle.net/11449/13029210.3892/ol.2014.2392WOS:000342725400009WOS000342725400009.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOncology Letters1.6640,599info:eu-repo/semantics/openAccess2024-01-01T06:15:29Zoai:repositorio.unesp.br:11449/130292Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:49:25.994954Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Phospho-mTOR in non-tumour and tumour bladder urothelium: Pattern of expression and impact on urothelial bladder cancer patients |
title |
Phospho-mTOR in non-tumour and tumour bladder urothelium: Pattern of expression and impact on urothelial bladder cancer patients |
spellingShingle |
Phospho-mTOR in non-tumour and tumour bladder urothelium: Pattern of expression and impact on urothelial bladder cancer patients Afonso, Julieta p-mTOR urothelial bladder cancer pattern of expression umbrella cells |
title_short |
Phospho-mTOR in non-tumour and tumour bladder urothelium: Pattern of expression and impact on urothelial bladder cancer patients |
title_full |
Phospho-mTOR in non-tumour and tumour bladder urothelium: Pattern of expression and impact on urothelial bladder cancer patients |
title_fullStr |
Phospho-mTOR in non-tumour and tumour bladder urothelium: Pattern of expression and impact on urothelial bladder cancer patients |
title_full_unstemmed |
Phospho-mTOR in non-tumour and tumour bladder urothelium: Pattern of expression and impact on urothelial bladder cancer patients |
title_sort |
Phospho-mTOR in non-tumour and tumour bladder urothelium: Pattern of expression and impact on urothelial bladder cancer patients |
author |
Afonso, Julieta |
author_facet |
Afonso, Julieta Longatto-Filho, Adhemar [UNESP] Da Silva, Vitor Moreira Amaro, Teresina Santos, Iucio L. |
author_role |
author |
author2 |
Longatto-Filho, Adhemar [UNESP] Da Silva, Vitor Moreira Amaro, Teresina Santos, Iucio L. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Univ Minho ICVS 3Bs Universidade Estadual Paulista (Unesp) Barretos Canc Hosp IPO Univ Fernando Pessoa |
dc.contributor.author.fl_str_mv |
Afonso, Julieta Longatto-Filho, Adhemar [UNESP] Da Silva, Vitor Moreira Amaro, Teresina Santos, Iucio L. |
dc.subject.por.fl_str_mv |
p-mTOR urothelial bladder cancer pattern of expression umbrella cells |
topic |
p-mTOR urothelial bladder cancer pattern of expression umbrella cells |
description |
Urothelial bladder carcinoma (UBC) is heterogeneous in its pathology and clinical behaviour. Evaluation of prognostic and predictive biomarkers is necessary, in order to produce personalised treatment options. The present study used immunohistochemistry to evaluate UBC sections containing tumour and non-tumour areas from 76 patients, for the detection of p-mTOR, CD31 and D2-40 (blood and lymphatic vessels identification, respectively). Of the non-tumour and tumour sections, 36 and 20% were scored positive for p-mTOR expression, respectively. Immunoexpression was observed in umbrella cells from non-tumour urothelium, in all cell layers from non-muscle-invasive (NMI) tumours (including expression in superficial cells), and in spots of cells from muscle-invasive (MI) tumours. Positive expression decreased from non-tumour to tumour urothelium, and from pTl/pTis to pT3/pT4 tumours; however, the few pT3/pT4 positive cases had worse survival rates, with 5-year disease-free survival being significantly lower. Angiogenesis occurrence was impaired in pT3/pT4 tumours that did not express p-mTOR. In conclusion, p-mTOR expression in non-tumour umbrella cells is likely a reflection of their metabolic plasticity, and extension to the inner layers of the urothelium in NMI tumours is consistent with an enhanced malignant potential. The expression in cell spots in a few MI tumours and absence of expression in the remaining tumours is intriguing and requires further research. Additional studies regarding the up- and downstream effectors of the mTOR pathway should be conducted. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10-01 2015-11-03T18:06:25Z 2015-11-03T18:06:25Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.spandidos-publications.com/10.3892/ol.2014.2392 Oncology Letters. Athens: Spandidos Publ Ltd, v. 8, n. 4, p. 1447-1454, 2014. 1792-1074 http://hdl.handle.net/11449/130292 10.3892/ol.2014.2392 WOS:000342725400009 WOS000342725400009.pdf |
url |
http://www.spandidos-publications.com/10.3892/ol.2014.2392 http://hdl.handle.net/11449/130292 |
identifier_str_mv |
Oncology Letters. Athens: Spandidos Publ Ltd, v. 8, n. 4, p. 1447-1454, 2014. 1792-1074 10.3892/ol.2014.2392 WOS:000342725400009 WOS000342725400009.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Oncology Letters 1.664 0,599 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1447-1454 application/pdf |
dc.publisher.none.fl_str_mv |
Spandidos Publ Ltd |
publisher.none.fl_str_mv |
Spandidos Publ Ltd |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129362344542208 |