First Microbiological and Molecular Identification of Rhodococcus equi in Feces of Nondiarrheic Cats
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2019/4278598 http://hdl.handle.net/11449/189551 |
Resumo: | Rhodococcus equi is responsible for infections in multiple-host animals. In humans, the prevalence of rhodococcus has increased worldwide and represents an emergent risk. R. equi is a soil-borne opportunistic bacterium isolated from feces of a wide variety of domestic species, except cats; thus, there is no known potential risk of its transmission from humans. Here, the mono- and cooccurrence of Rhodococcus equi and other bacteria and selected virulence markers were investigated in feces of nondiarrheic cats from urban (n=100) and rural (n=100) areas. Seven (7/200=3.5%) R. equi isolates were recovered in ceftazidime, novobiocin, and cycloheximide (CAZ-NB) selective media, exclusively of cats from three distinct farms (p=0.01), and these cats had a history of contact with horses and their environment (p=0.0002). None of the R. equi isolates harbored hosted-adapted plasmid types associated with virulence (pVAPA, pVAPB, and pVAPN). One hundred seventy-five E. coli isolates were identified, and 23 atypical enteropathogenic E. coli (aEPEC), 1 STEC (Shiga-toxin producing E. coli), and 1 EAEC (enteroaggregative E. coli) were detected. Eighty-six C. perfringens type A isolates were identified, and beta-2 and enterotoxin were detected in 21 and 1 isolates, respectively. Five C. difficile isolates were identified, one of which was toxigenic and ribotype 106. The main cooccurring isolates in cats from urban areas were E. coli and C. perfringens A (26/100=26%), E. coli and C. perfringens type A cpb2+ (8/100=8%), and aEPEC (eae+/escN+) and C. perfringens type A (5/100=5%). In cats from farms, the main cooccurring isolates were E. coli and C. perfringens type A (21/100=21%), E. coli and C. perfringens type A cpb2+ 8/100=8%), and E. coli and R. equi (4/100=4%). We identified, for the first time, R. equi in nondiarrheic cats, a finding that represents a public health issue because rhodococcus has been reported in both immunosuppressed and immunocompetent humans, particularly people living with HIV/AIDS. |
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First Microbiological and Molecular Identification of Rhodococcus equi in Feces of Nondiarrheic CatsRhodococcus equi is responsible for infections in multiple-host animals. In humans, the prevalence of rhodococcus has increased worldwide and represents an emergent risk. R. equi is a soil-borne opportunistic bacterium isolated from feces of a wide variety of domestic species, except cats; thus, there is no known potential risk of its transmission from humans. Here, the mono- and cooccurrence of Rhodococcus equi and other bacteria and selected virulence markers were investigated in feces of nondiarrheic cats from urban (n=100) and rural (n=100) areas. Seven (7/200=3.5%) R. equi isolates were recovered in ceftazidime, novobiocin, and cycloheximide (CAZ-NB) selective media, exclusively of cats from three distinct farms (p=0.01), and these cats had a history of contact with horses and their environment (p=0.0002). None of the R. equi isolates harbored hosted-adapted plasmid types associated with virulence (pVAPA, pVAPB, and pVAPN). One hundred seventy-five E. coli isolates were identified, and 23 atypical enteropathogenic E. coli (aEPEC), 1 STEC (Shiga-toxin producing E. coli), and 1 EAEC (enteroaggregative E. coli) were detected. Eighty-six C. perfringens type A isolates were identified, and beta-2 and enterotoxin were detected in 21 and 1 isolates, respectively. Five C. difficile isolates were identified, one of which was toxigenic and ribotype 106. The main cooccurring isolates in cats from urban areas were E. coli and C. perfringens A (26/100=26%), E. coli and C. perfringens type A cpb2+ (8/100=8%), and aEPEC (eae+/escN+) and C. perfringens type A (5/100=5%). In cats from farms, the main cooccurring isolates were E. coli and C. perfringens type A (21/100=21%), E. coli and C. perfringens type A cpb2+ 8/100=8%), and E. coli and R. equi (4/100=4%). We identified, for the first time, R. equi in nondiarrheic cats, a finding that represents a public health issue because rhodococcus has been reported in both immunosuppressed and immunocompetent humans, particularly people living with HIV/AIDS.Department of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Sciences UNESP-São Paulo State UniversityDepartment of Preventive Veterinary Medicine Federal University of Minas Gerais UFMGDepartment of Microbiology and Immunology Biosciences Institute UNESPTechnology Faculty FATECPaulista University UNIPSchool of Veterinary Medicine University of Oeste Paulista UNOESTESchool of Veterinary Medicine Kitasato UniversityDepartment of Veterinary Hygiene and Public Health School of Veterinary Medicine and Animal Sciences UNESP-São Paulo State UniversityDepartment of Microbiology and Immunology Biosciences Institute UNESPUniversidade Estadual Paulista (Unesp)Universidade Federal de Minas Gerais (UFMG)FATECUNIPUNOESTEKitasato UniversityLechinski de Paula, Carolina [UNESP]Silveira Silva, Rodrigo OtávioTavanelli Hernandes, Rodrigo [UNESP]de Nardi Júnior, GeraldoBabboni, Selene DanielaTrevizan Guerra, Simony [UNESP]Paganini Listoni, Fernando José [UNESP]Giuffrida, RogérioTakai, ShinjiSasaki, YukakoGarcia Ribeiro, Márcio [UNESP]2019-10-06T16:44:22Z2019-10-06T16:44:22Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4278598http://dx.doi.org/10.1155/2019/4278598BioMed research international, v. 2019, p. 4278598-.2314-6141http://hdl.handle.net/11449/18955110.1155/2019/42785982-s2.0-85071154875Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioMed research internationalinfo:eu-repo/semantics/openAccess2024-09-03T14:11:31Zoai:repositorio.unesp.br:11449/189551Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T14:11:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
First Microbiological and Molecular Identification of Rhodococcus equi in Feces of Nondiarrheic Cats |
title |
First Microbiological and Molecular Identification of Rhodococcus equi in Feces of Nondiarrheic Cats |
spellingShingle |
First Microbiological and Molecular Identification of Rhodococcus equi in Feces of Nondiarrheic Cats Lechinski de Paula, Carolina [UNESP] |
title_short |
First Microbiological and Molecular Identification of Rhodococcus equi in Feces of Nondiarrheic Cats |
title_full |
First Microbiological and Molecular Identification of Rhodococcus equi in Feces of Nondiarrheic Cats |
title_fullStr |
First Microbiological and Molecular Identification of Rhodococcus equi in Feces of Nondiarrheic Cats |
title_full_unstemmed |
First Microbiological and Molecular Identification of Rhodococcus equi in Feces of Nondiarrheic Cats |
title_sort |
First Microbiological and Molecular Identification of Rhodococcus equi in Feces of Nondiarrheic Cats |
author |
Lechinski de Paula, Carolina [UNESP] |
author_facet |
Lechinski de Paula, Carolina [UNESP] Silveira Silva, Rodrigo Otávio Tavanelli Hernandes, Rodrigo [UNESP] de Nardi Júnior, Geraldo Babboni, Selene Daniela Trevizan Guerra, Simony [UNESP] Paganini Listoni, Fernando José [UNESP] Giuffrida, Rogério Takai, Shinji Sasaki, Yukako Garcia Ribeiro, Márcio [UNESP] |
author_role |
author |
author2 |
Silveira Silva, Rodrigo Otávio Tavanelli Hernandes, Rodrigo [UNESP] de Nardi Júnior, Geraldo Babboni, Selene Daniela Trevizan Guerra, Simony [UNESP] Paganini Listoni, Fernando José [UNESP] Giuffrida, Rogério Takai, Shinji Sasaki, Yukako Garcia Ribeiro, Márcio [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Minas Gerais (UFMG) FATEC UNIP UNOESTE Kitasato University |
dc.contributor.author.fl_str_mv |
Lechinski de Paula, Carolina [UNESP] Silveira Silva, Rodrigo Otávio Tavanelli Hernandes, Rodrigo [UNESP] de Nardi Júnior, Geraldo Babboni, Selene Daniela Trevizan Guerra, Simony [UNESP] Paganini Listoni, Fernando José [UNESP] Giuffrida, Rogério Takai, Shinji Sasaki, Yukako Garcia Ribeiro, Márcio [UNESP] |
description |
Rhodococcus equi is responsible for infections in multiple-host animals. In humans, the prevalence of rhodococcus has increased worldwide and represents an emergent risk. R. equi is a soil-borne opportunistic bacterium isolated from feces of a wide variety of domestic species, except cats; thus, there is no known potential risk of its transmission from humans. Here, the mono- and cooccurrence of Rhodococcus equi and other bacteria and selected virulence markers were investigated in feces of nondiarrheic cats from urban (n=100) and rural (n=100) areas. Seven (7/200=3.5%) R. equi isolates were recovered in ceftazidime, novobiocin, and cycloheximide (CAZ-NB) selective media, exclusively of cats from three distinct farms (p=0.01), and these cats had a history of contact with horses and their environment (p=0.0002). None of the R. equi isolates harbored hosted-adapted plasmid types associated with virulence (pVAPA, pVAPB, and pVAPN). One hundred seventy-five E. coli isolates were identified, and 23 atypical enteropathogenic E. coli (aEPEC), 1 STEC (Shiga-toxin producing E. coli), and 1 EAEC (enteroaggregative E. coli) were detected. Eighty-six C. perfringens type A isolates were identified, and beta-2 and enterotoxin were detected in 21 and 1 isolates, respectively. Five C. difficile isolates were identified, one of which was toxigenic and ribotype 106. The main cooccurring isolates in cats from urban areas were E. coli and C. perfringens A (26/100=26%), E. coli and C. perfringens type A cpb2+ (8/100=8%), and aEPEC (eae+/escN+) and C. perfringens type A (5/100=5%). In cats from farms, the main cooccurring isolates were E. coli and C. perfringens type A (21/100=21%), E. coli and C. perfringens type A cpb2+ 8/100=8%), and E. coli and R. equi (4/100=4%). We identified, for the first time, R. equi in nondiarrheic cats, a finding that represents a public health issue because rhodococcus has been reported in both immunosuppressed and immunocompetent humans, particularly people living with HIV/AIDS. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:44:22Z 2019-10-06T16:44:22Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2019/4278598 BioMed research international, v. 2019, p. 4278598-. 2314-6141 http://hdl.handle.net/11449/189551 10.1155/2019/4278598 2-s2.0-85071154875 |
url |
http://dx.doi.org/10.1155/2019/4278598 http://hdl.handle.net/11449/189551 |
identifier_str_mv |
BioMed research international, v. 2019, p. 4278598-. 2314-6141 10.1155/2019/4278598 2-s2.0-85071154875 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
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BioMed research international |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
4278598 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
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UNESP |
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UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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repositoriounesp@unesp.br |
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1810021371991818240 |