A new experimental model of cigarette smoke-induced emphysema in Wistar rats

• Autor(a) principal: las Heras Kozma, Rodrigo de
• Data de Publicação: 2014
• Outros Autores: Alves, Edson Marcelino [UNESP], Barbosa-de-Oliveira, Valter Abraao, Santos, Lucinéia dos [UNESP], Guardia, Renan Cenize, Buzo, Henrique Vivi, Faria, Carolina Arruda de, Yamashita, Camila [UNESP], Cavazzana Junior, Manzelio, Frei, Fernando [UNESP], Oliveira Ribeiro-Paes, Maria Jose de, Ribeiro-Paes, Joao Tadeu [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• DOI: 10.1590/S1806-37132014000100007
• Texto Completo: http://dx.doi.org/10.1590/S1806-37132014000100007; http://hdl.handle.net/11449/112046
• topic: Tobacco smoke pollution; Emphysema; Disease models, animal; Equipment and supplies

Objective: To describe a new murine model of cigarette smoke-induced emphysema. Methods: Twenty-four male Wistar rats were divided into two groups: the cigarette smoke group, comprising 12 rats exposed to smoke from 12 commercial filter cigarettes three times a day (a total of 36 cigarettes per day) every day for 30 weeks; and the control group, comprising 12 rats exposed to room air three times a day every day for 30 weeks. Lung function was assessed by mechanical ventilation, and emphysema was morphometrically assessed by measurement of the mean linear intercept (Lm). Results: The mean weight gain was significantly (approximately ten times) lower in the cigarette smoke group than in the control group. The Lm was 25.0% higher in the cigarette smoke group. There was a trend toward worsening of lung function parameters in the cigarette smoke group. Conclusions: The new murine model of cigarette smoke-induced emphysema and the methodology employed in the present study are effective and reproducible, representing a promising and economically viable option for use in studies investigating the pathophysiology of and therapeutic approaches to COPD.