Effects of the Latex of Synadenium grantii Hook F. (Euphorbiaceae) on a Preclinical Model of Canine Prostate Cancer

Detalhes bibliográficos
Autor(a) principal: Brito, Eric Saymom Andrade
Data de Publicação: 2021
Outros Autores: Prado, Laís Di Paulie Taborda, Araújo, Liana Késia Costa, Arnhold, Emmanuel, Matos, Moema Pacheco Chediak, de Paula, Joelma Abadia Marciano, Ramos, Luciana Machado, Fonseca-Alves, Carlos Eduardo [UNESP], de Moura, Veridiana Maria Brianezi Dignani
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3389/fvets.2021.605286
http://hdl.handle.net/11449/206264
Resumo: Prostatic cancer (PC) stands out in terms of its occurrence, pathophysiology, and unfavorable prognostics in humans and dogs. Natural drugs bear an integrative potential for conventional antineoplastic treatments. In this context, the bioproducts of Synadenium grantii have been empirically used in different parts of Brazil for the integrative treatment of prostate cancer in humans. However, there is no availability of scientific evidence of the antitumor effects of S. grantii. Therefore, this study aimed to investigate the bioactive compounds in the latex of S. grantii using the high-resolution mass spectrophotometry (HRMS) and to evaluate its cytotoxic effects on primary canine PC cell cultures. Four fragments of phorbol ester were identified as potential bioactive compounds using the HRMS. With the help of an MTT ([3-(4,5-dimethyldiazol-2-yl)-2,5 diphenyltetrazolium bromide]) assay, two canine prostatic carcinoma cell lines (PC 1 and PC2) showed a decrease in the tumor cell count, with an Inhibitory concentration 50 (IC50)of 0.8469 and 0.6068 mg/ml, respectively, for PC1 and PC2. In conclusion, the latex of S. grantii contains phorbol esters in its composition, and its aqueous solution has a cytotoxic effect on canine metastatic PC cells in vitro.
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spelling Effects of the Latex of Synadenium grantii Hook F. (Euphorbiaceae) on a Preclinical Model of Canine Prostate Cancercell culturedogjanaubaneoplasiaprostateprostate cancer modelProstatic cancer (PC) stands out in terms of its occurrence, pathophysiology, and unfavorable prognostics in humans and dogs. Natural drugs bear an integrative potential for conventional antineoplastic treatments. In this context, the bioproducts of Synadenium grantii have been empirically used in different parts of Brazil for the integrative treatment of prostate cancer in humans. However, there is no availability of scientific evidence of the antitumor effects of S. grantii. Therefore, this study aimed to investigate the bioactive compounds in the latex of S. grantii using the high-resolution mass spectrophotometry (HRMS) and to evaluate its cytotoxic effects on primary canine PC cell cultures. Four fragments of phorbol ester were identified as potential bioactive compounds using the HRMS. With the help of an MTT ([3-(4,5-dimethyldiazol-2-yl)-2,5 diphenyltetrazolium bromide]) assay, two canine prostatic carcinoma cell lines (PC 1 and PC2) showed a decrease in the tumor cell count, with an Inhibitory concentration 50 (IC50)of 0.8469 and 0.6068 mg/ml, respectively, for PC1 and PC2. In conclusion, the latex of S. grantii contains phorbol esters in its composition, and its aqueous solution has a cytotoxic effect on canine metastatic PC cells in vitro.Animal Pathology Service Federal University of Goiás- UFGDepartment of Animal Science Federal University of Goiás UFGUnit of Exact and Technologic Sciences Goiás State UniversityDepartment of Veterinary Surgery and Animal Reproduction São Paulo State University-UNESPInstitute of Health Sciences University of São Paulo—UNIPDepartment of Veterinary Surgery and Animal Reproduction São Paulo State University-UNESPUniversidade Federal de Goiás (UFG)Goiás State UniversityUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Brito, Eric Saymom AndradePrado, Laís Di Paulie TabordaAraújo, Liana Késia CostaArnhold, EmmanuelMatos, Moema Pacheco Chediakde Paula, Joelma Abadia MarcianoRamos, Luciana MachadoFonseca-Alves, Carlos Eduardo [UNESP]de Moura, Veridiana Maria Brianezi Dignani2021-06-25T10:29:13Z2021-06-25T10:29:13Z2021-04-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fvets.2021.605286Frontiers in Veterinary Science, v. 8.2297-1769http://hdl.handle.net/11449/20626410.3389/fvets.2021.6052862-s2.0-85105018541Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Veterinary Scienceinfo:eu-repo/semantics/openAccess2021-10-23T02:05:50Zoai:repositorio.unesp.br:11449/206264Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:00:26.426070Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effects of the Latex of Synadenium grantii Hook F. (Euphorbiaceae) on a Preclinical Model of Canine Prostate Cancer
title Effects of the Latex of Synadenium grantii Hook F. (Euphorbiaceae) on a Preclinical Model of Canine Prostate Cancer
spellingShingle Effects of the Latex of Synadenium grantii Hook F. (Euphorbiaceae) on a Preclinical Model of Canine Prostate Cancer
Brito, Eric Saymom Andrade
cell culture
dog
janauba
neoplasia
prostate
prostate cancer model
title_short Effects of the Latex of Synadenium grantii Hook F. (Euphorbiaceae) on a Preclinical Model of Canine Prostate Cancer
title_full Effects of the Latex of Synadenium grantii Hook F. (Euphorbiaceae) on a Preclinical Model of Canine Prostate Cancer
title_fullStr Effects of the Latex of Synadenium grantii Hook F. (Euphorbiaceae) on a Preclinical Model of Canine Prostate Cancer
title_full_unstemmed Effects of the Latex of Synadenium grantii Hook F. (Euphorbiaceae) on a Preclinical Model of Canine Prostate Cancer
title_sort Effects of the Latex of Synadenium grantii Hook F. (Euphorbiaceae) on a Preclinical Model of Canine Prostate Cancer
author Brito, Eric Saymom Andrade
author_facet Brito, Eric Saymom Andrade
Prado, Laís Di Paulie Taborda
Araújo, Liana Késia Costa
Arnhold, Emmanuel
Matos, Moema Pacheco Chediak
de Paula, Joelma Abadia Marciano
Ramos, Luciana Machado
Fonseca-Alves, Carlos Eduardo [UNESP]
de Moura, Veridiana Maria Brianezi Dignani
author_role author
author2 Prado, Laís Di Paulie Taborda
Araújo, Liana Késia Costa
Arnhold, Emmanuel
Matos, Moema Pacheco Chediak
de Paula, Joelma Abadia Marciano
Ramos, Luciana Machado
Fonseca-Alves, Carlos Eduardo [UNESP]
de Moura, Veridiana Maria Brianezi Dignani
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Goiás (UFG)
Goiás State University
Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Brito, Eric Saymom Andrade
Prado, Laís Di Paulie Taborda
Araújo, Liana Késia Costa
Arnhold, Emmanuel
Matos, Moema Pacheco Chediak
de Paula, Joelma Abadia Marciano
Ramos, Luciana Machado
Fonseca-Alves, Carlos Eduardo [UNESP]
de Moura, Veridiana Maria Brianezi Dignani
dc.subject.por.fl_str_mv cell culture
dog
janauba
neoplasia
prostate
prostate cancer model
topic cell culture
dog
janauba
neoplasia
prostate
prostate cancer model
description Prostatic cancer (PC) stands out in terms of its occurrence, pathophysiology, and unfavorable prognostics in humans and dogs. Natural drugs bear an integrative potential for conventional antineoplastic treatments. In this context, the bioproducts of Synadenium grantii have been empirically used in different parts of Brazil for the integrative treatment of prostate cancer in humans. However, there is no availability of scientific evidence of the antitumor effects of S. grantii. Therefore, this study aimed to investigate the bioactive compounds in the latex of S. grantii using the high-resolution mass spectrophotometry (HRMS) and to evaluate its cytotoxic effects on primary canine PC cell cultures. Four fragments of phorbol ester were identified as potential bioactive compounds using the HRMS. With the help of an MTT ([3-(4,5-dimethyldiazol-2-yl)-2,5 diphenyltetrazolium bromide]) assay, two canine prostatic carcinoma cell lines (PC 1 and PC2) showed a decrease in the tumor cell count, with an Inhibitory concentration 50 (IC50)of 0.8469 and 0.6068 mg/ml, respectively, for PC1 and PC2. In conclusion, the latex of S. grantii contains phorbol esters in its composition, and its aqueous solution has a cytotoxic effect on canine metastatic PC cells in vitro.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:29:13Z
2021-06-25T10:29:13Z
2021-04-12
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3389/fvets.2021.605286
Frontiers in Veterinary Science, v. 8.
2297-1769
http://hdl.handle.net/11449/206264
10.3389/fvets.2021.605286
2-s2.0-85105018541
url http://dx.doi.org/10.3389/fvets.2021.605286
http://hdl.handle.net/11449/206264
identifier_str_mv Frontiers in Veterinary Science, v. 8.
2297-1769
10.3389/fvets.2021.605286
2-s2.0-85105018541
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frontiers in Veterinary Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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