Effect of an acrylic resin combined with an antimicrobial polymer on biofilm formation
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-77572012000600009 http://hdl.handle.net/11449/227110 |
Resumo: | Objectives: The purpose of this study was to evaluate the antimicrobial activity of an acrylic resin combined with an antimicrobial polymer poly (2-tert-butylaminoethyl) methacrylate (PTBAEMA) to inhibit Staphylococcus aureus, Streptococcus mutans and Candida albicans biofilm formation. Material and Methods: Discs of a heat-polymerized acrylic resin were produced and divided according to PTBAEMA concentration: 0 (control), 10 and 25%. The specimens were inoculated (107 CFU/mL) and incubated at 37°C for 48 h. After incubation, the wells were washed and each specimen was sonicated for 20 min. Replicate aliquots of resultant suspensions were plated at dilutions at 37°C for 48 h. The number of colony-forming units (CFU) was counted and expressed as log (CFU+1)/mL and analyzed statistically with α=.05. Results: The results showed that 25% PTBAEMA completely inhibited S. aureus and S. mutans biofilm formation. A significant reduction of log (CFU+1)/mL in count of S. aureus (control: 7.9±0.8A; 10%: 3.8±3.3B) and S. mutans (control: 7.5±0.7A; 10%: 5.1±2.7B) was observed for the group containing 10% PTBAEMA (Mann-Whitney, p<0.05). For C. albicans, differences were not significant among the groups (control: 6.6±0.2A; 10%: 6.6±0.4A; 25%: 6.4±0.1A), (Kruskal-Wallis, p>0.05, P=0.079). Conclusions: Acrylic resin combined with 10 and 25% of PTBAEMA showed significant antimicrobial activity against S. aureus and S. mutans biofilm, but it was inactive against the C. albicans biofilm. |
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Effect of an acrylic resin combined with an antimicrobial polymer on biofilm formationAcrylic resinsBiofilmsDental materialsPolymersObjectives: The purpose of this study was to evaluate the antimicrobial activity of an acrylic resin combined with an antimicrobial polymer poly (2-tert-butylaminoethyl) methacrylate (PTBAEMA) to inhibit Staphylococcus aureus, Streptococcus mutans and Candida albicans biofilm formation. Material and Methods: Discs of a heat-polymerized acrylic resin were produced and divided according to PTBAEMA concentration: 0 (control), 10 and 25%. The specimens were inoculated (107 CFU/mL) and incubated at 37°C for 48 h. After incubation, the wells were washed and each specimen was sonicated for 20 min. Replicate aliquots of resultant suspensions were plated at dilutions at 37°C for 48 h. The number of colony-forming units (CFU) was counted and expressed as log (CFU+1)/mL and analyzed statistically with α=.05. Results: The results showed that 25% PTBAEMA completely inhibited S. aureus and S. mutans biofilm formation. A significant reduction of log (CFU+1)/mL in count of S. aureus (control: 7.9±0.8A; 10%: 3.8±3.3B) and S. mutans (control: 7.5±0.7A; 10%: 5.1±2.7B) was observed for the group containing 10% PTBAEMA (Mann-Whitney, p<0.05). For C. albicans, differences were not significant among the groups (control: 6.6±0.2A; 10%: 6.6±0.4A; 25%: 6.4±0.1A), (Kruskal-Wallis, p>0.05, P=0.079). Conclusions: Acrylic resin combined with 10 and 25% of PTBAEMA showed significant antimicrobial activity against S. aureus and S. mutans biofilm, but it was inactive against the C. albicans biofilm.Department of Prosthodontics Paulista University-UNIP, Goiânia, GODepartment of Dental Materials and Prosthodontics Araraquara Dental School Univ. Estadual Paulista-UNESP, Rua Humaitá, 1680, 14801-903-Araraquara-SPDepartment of Dental Materials and Prosthodontics Araraquara Dental School Univ. Estadual Paulista-UNESP, Rua Humaitá, 1680, 14801-903-Araraquara-SPPaulista University-UNIPUniversidade Estadual Paulista (UNESP)Marra, JuliêPaleari, André Gustavo [UNESP]Rodriguez, Larissa Santana [UNESP]Leite, Andressa Rosa Perin [UNESP]Pero, Ana Carolina [UNESP]Compagnoni, Marco Antonio [UNESP]2022-04-29T06:48:18Z2022-04-29T06:48:18Z2012-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article643-648http://dx.doi.org/10.1590/S1678-77572012000600009Journal of Applied Oral Science, v. 20, n. 6, p. 643-648, 2012.1678-77651678-7757http://hdl.handle.net/11449/22711010.1590/S1678-775720120006000092-s2.0-84872818467Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Oral Scienceinfo:eu-repo/semantics/openAccess2022-04-29T06:48:18Zoai:repositorio.unesp.br:11449/227110Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:00:47.240079Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of an acrylic resin combined with an antimicrobial polymer on biofilm formation |
title |
Effect of an acrylic resin combined with an antimicrobial polymer on biofilm formation |
spellingShingle |
Effect of an acrylic resin combined with an antimicrobial polymer on biofilm formation Marra, Juliê Acrylic resins Biofilms Dental materials Polymers |
title_short |
Effect of an acrylic resin combined with an antimicrobial polymer on biofilm formation |
title_full |
Effect of an acrylic resin combined with an antimicrobial polymer on biofilm formation |
title_fullStr |
Effect of an acrylic resin combined with an antimicrobial polymer on biofilm formation |
title_full_unstemmed |
Effect of an acrylic resin combined with an antimicrobial polymer on biofilm formation |
title_sort |
Effect of an acrylic resin combined with an antimicrobial polymer on biofilm formation |
author |
Marra, Juliê |
author_facet |
Marra, Juliê Paleari, André Gustavo [UNESP] Rodriguez, Larissa Santana [UNESP] Leite, Andressa Rosa Perin [UNESP] Pero, Ana Carolina [UNESP] Compagnoni, Marco Antonio [UNESP] |
author_role |
author |
author2 |
Paleari, André Gustavo [UNESP] Rodriguez, Larissa Santana [UNESP] Leite, Andressa Rosa Perin [UNESP] Pero, Ana Carolina [UNESP] Compagnoni, Marco Antonio [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Paulista University-UNIP Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Marra, Juliê Paleari, André Gustavo [UNESP] Rodriguez, Larissa Santana [UNESP] Leite, Andressa Rosa Perin [UNESP] Pero, Ana Carolina [UNESP] Compagnoni, Marco Antonio [UNESP] |
dc.subject.por.fl_str_mv |
Acrylic resins Biofilms Dental materials Polymers |
topic |
Acrylic resins Biofilms Dental materials Polymers |
description |
Objectives: The purpose of this study was to evaluate the antimicrobial activity of an acrylic resin combined with an antimicrobial polymer poly (2-tert-butylaminoethyl) methacrylate (PTBAEMA) to inhibit Staphylococcus aureus, Streptococcus mutans and Candida albicans biofilm formation. Material and Methods: Discs of a heat-polymerized acrylic resin were produced and divided according to PTBAEMA concentration: 0 (control), 10 and 25%. The specimens were inoculated (107 CFU/mL) and incubated at 37°C for 48 h. After incubation, the wells were washed and each specimen was sonicated for 20 min. Replicate aliquots of resultant suspensions were plated at dilutions at 37°C for 48 h. The number of colony-forming units (CFU) was counted and expressed as log (CFU+1)/mL and analyzed statistically with α=.05. Results: The results showed that 25% PTBAEMA completely inhibited S. aureus and S. mutans biofilm formation. A significant reduction of log (CFU+1)/mL in count of S. aureus (control: 7.9±0.8A; 10%: 3.8±3.3B) and S. mutans (control: 7.5±0.7A; 10%: 5.1±2.7B) was observed for the group containing 10% PTBAEMA (Mann-Whitney, p<0.05). For C. albicans, differences were not significant among the groups (control: 6.6±0.2A; 10%: 6.6±0.4A; 25%: 6.4±0.1A), (Kruskal-Wallis, p>0.05, P=0.079). Conclusions: Acrylic resin combined with 10 and 25% of PTBAEMA showed significant antimicrobial activity against S. aureus and S. mutans biofilm, but it was inactive against the C. albicans biofilm. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 2022-04-29T06:48:18Z 2022-04-29T06:48:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-77572012000600009 Journal of Applied Oral Science, v. 20, n. 6, p. 643-648, 2012. 1678-7765 1678-7757 http://hdl.handle.net/11449/227110 10.1590/S1678-77572012000600009 2-s2.0-84872818467 |
url |
http://dx.doi.org/10.1590/S1678-77572012000600009 http://hdl.handle.net/11449/227110 |
identifier_str_mv |
Journal of Applied Oral Science, v. 20, n. 6, p. 643-648, 2012. 1678-7765 1678-7757 10.1590/S1678-77572012000600009 2-s2.0-84872818467 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Applied Oral Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
643-648 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128884378435584 |