First detection of canine parvovirus 2b DNA in a crab-eating fox pup (Cerdocyon thous, Linnaeus, 1766)
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s42770-020-00389-9 http://hdl.handle.net/11449/209607 |
Resumo: | The crab-eating fox (Cerdocyon thous) is a small wild mammal present in all Brazilian biomes and in some countries of South America. This study aimed to verify the involvement of viral infectious agents in the death of a wild crab-eating fox pup (Cerdocyon thous) in Brazil. The Center for Medicine and Research of Wild Animals of the Universidade Estadual Paulista received a free-living crab-eating fox aged approximately 21 days and apparently healthy. After 13 days, the animal presented anorexia, diarrhea, fever, prostration, and neurological signs progressing to death with an inconclusive diagnosis. In a retrospective study, tissue fragments stored at - 80 degrees C were used to identify nucleic acids from major canine viruses, such as canine parvovirus-2 (CPV-2), canine adenovirus A types 1 and 2, canid alphaherpesvirus 1, and canine distemper virus. The amplified product with the expected length for CPV-2 was obtained from the heart fragment. After performing nucleotide (nt) sequencing of the amplicon, it was possible to demonstrate that the crab-eating fox strain exhibited high (99.8%) nt identity with the CPV-2b prototype (CPV-39 strain). Additionally, deduced amino acid (aa) sequence analysis showed the GAT codon for the aa Asp (D) at position 426 of the CPV-2 viral protein VP2, which characterizes the subtype 2b. To the best of the authors' knowledge, this report describes the first detection of CPV-2b DNA in tissue fragments from a crab-eating fox. |
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First detection of canine parvovirus 2b DNA in a crab-eating fox pup (Cerdocyon thous, Linnaeus, 1766)Cerdocyon thousWildHeartCPV-2bThe crab-eating fox (Cerdocyon thous) is a small wild mammal present in all Brazilian biomes and in some countries of South America. This study aimed to verify the involvement of viral infectious agents in the death of a wild crab-eating fox pup (Cerdocyon thous) in Brazil. The Center for Medicine and Research of Wild Animals of the Universidade Estadual Paulista received a free-living crab-eating fox aged approximately 21 days and apparently healthy. After 13 days, the animal presented anorexia, diarrhea, fever, prostration, and neurological signs progressing to death with an inconclusive diagnosis. In a retrospective study, tissue fragments stored at - 80 degrees C were used to identify nucleic acids from major canine viruses, such as canine parvovirus-2 (CPV-2), canine adenovirus A types 1 and 2, canid alphaherpesvirus 1, and canine distemper virus. The amplified product with the expected length for CPV-2 was obtained from the heart fragment. After performing nucleotide (nt) sequencing of the amplicon, it was possible to demonstrate that the crab-eating fox strain exhibited high (99.8%) nt identity with the CPV-2b prototype (CPV-39 strain). Additionally, deduced amino acid (aa) sequence analysis showed the GAT codon for the aa Asp (D) at position 426 of the CPV-2 viral protein VP2, which characterizes the subtype 2b. To the best of the authors' knowledge, this report describes the first detection of CPV-2b DNA in tissue fragments from a crab-eating fox.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Brazilian institute: Financing of Studies and Projects (FINEP)Brazilian institute: Araucaria Foundation (FAP/PR)FunadespUniv Estadual Londrina, Lab Anim Virol, Dept Vet Prevent Med, Celso Garcia Cid Rd,PR455 Km 380,POB 10011, BR-86057970 Londrina, Parana, BrazilUniv Estadual Londrina, Multiuser Anim Hlth Lab, Mol Biol Unit, Dept Vet Prevent Med, Celso Garcia Cid Rd,PR455 Km 380,POB 10011, BR-86057970 Londrina, Parana, BrazilUniv Pitagoras Unopar, Post Grad Program Anim Hlth & Prod, Arapongas, Parana, BrazilUniv Estadual Paulista, Vet Hosp, Ctr Med & Res Wild Anim, POB 560, BR-18618000 Botucatu, SP, BrazilUniv Estadual Paulista, Vet Hosp, Anim Pathol, POB 560, BR-18618000 Botucatu, SP, BrazilUniv Estadual Paulista, Vet Hosp, Ctr Med & Res Wild Anim, POB 560, BR-18618000 Botucatu, SP, BrazilUniv Estadual Paulista, Vet Hosp, Anim Pathol, POB 560, BR-18618000 Botucatu, SP, BrazilSpringerUniversidade Estadual de Londrina (UEL)Univ Pitagoras UnoparUniversidade Estadual Paulista (Unesp)Spera, Caroline GiuseppaLavorente, Fernanda Louise PereiraLorenzetti, ElisCalasans Marques, Gustavo de [UNESP]Almeida Freitas, Luana deMartins, Maira Bonamin [UNESP]Teixeira, Carlos Roberto [UNESP]Alfieri, Amauri AlcindoAlfieri, Alice Fernandes2021-06-25T12:23:45Z2021-06-25T12:23:45Z2020-10-23info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article449-453http://dx.doi.org/10.1007/s42770-020-00389-9Brazilian Journal Of Microbiology. New York: Springer, v. 52, n. 1, p. 449-453, 2021.1517-8382http://hdl.handle.net/11449/20960710.1007/s42770-020-00389-9WOS:000583488700001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBrazilian Journal Of Microbiologyinfo:eu-repo/semantics/openAccess2024-09-03T13:15:27Zoai:repositorio.unesp.br:11449/209607Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:15:27Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
First detection of canine parvovirus 2b DNA in a crab-eating fox pup (Cerdocyon thous, Linnaeus, 1766) |
title |
First detection of canine parvovirus 2b DNA in a crab-eating fox pup (Cerdocyon thous, Linnaeus, 1766) |
spellingShingle |
First detection of canine parvovirus 2b DNA in a crab-eating fox pup (Cerdocyon thous, Linnaeus, 1766) Spera, Caroline Giuseppa Cerdocyon thous Wild Heart CPV-2b |
title_short |
First detection of canine parvovirus 2b DNA in a crab-eating fox pup (Cerdocyon thous, Linnaeus, 1766) |
title_full |
First detection of canine parvovirus 2b DNA in a crab-eating fox pup (Cerdocyon thous, Linnaeus, 1766) |
title_fullStr |
First detection of canine parvovirus 2b DNA in a crab-eating fox pup (Cerdocyon thous, Linnaeus, 1766) |
title_full_unstemmed |
First detection of canine parvovirus 2b DNA in a crab-eating fox pup (Cerdocyon thous, Linnaeus, 1766) |
title_sort |
First detection of canine parvovirus 2b DNA in a crab-eating fox pup (Cerdocyon thous, Linnaeus, 1766) |
author |
Spera, Caroline Giuseppa |
author_facet |
Spera, Caroline Giuseppa Lavorente, Fernanda Louise Pereira Lorenzetti, Elis Calasans Marques, Gustavo de [UNESP] Almeida Freitas, Luana de Martins, Maira Bonamin [UNESP] Teixeira, Carlos Roberto [UNESP] Alfieri, Amauri Alcindo Alfieri, Alice Fernandes |
author_role |
author |
author2 |
Lavorente, Fernanda Louise Pereira Lorenzetti, Elis Calasans Marques, Gustavo de [UNESP] Almeida Freitas, Luana de Martins, Maira Bonamin [UNESP] Teixeira, Carlos Roberto [UNESP] Alfieri, Amauri Alcindo Alfieri, Alice Fernandes |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Londrina (UEL) Univ Pitagoras Unopar Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Spera, Caroline Giuseppa Lavorente, Fernanda Louise Pereira Lorenzetti, Elis Calasans Marques, Gustavo de [UNESP] Almeida Freitas, Luana de Martins, Maira Bonamin [UNESP] Teixeira, Carlos Roberto [UNESP] Alfieri, Amauri Alcindo Alfieri, Alice Fernandes |
dc.subject.por.fl_str_mv |
Cerdocyon thous Wild Heart CPV-2b |
topic |
Cerdocyon thous Wild Heart CPV-2b |
description |
The crab-eating fox (Cerdocyon thous) is a small wild mammal present in all Brazilian biomes and in some countries of South America. This study aimed to verify the involvement of viral infectious agents in the death of a wild crab-eating fox pup (Cerdocyon thous) in Brazil. The Center for Medicine and Research of Wild Animals of the Universidade Estadual Paulista received a free-living crab-eating fox aged approximately 21 days and apparently healthy. After 13 days, the animal presented anorexia, diarrhea, fever, prostration, and neurological signs progressing to death with an inconclusive diagnosis. In a retrospective study, tissue fragments stored at - 80 degrees C were used to identify nucleic acids from major canine viruses, such as canine parvovirus-2 (CPV-2), canine adenovirus A types 1 and 2, canid alphaherpesvirus 1, and canine distemper virus. The amplified product with the expected length for CPV-2 was obtained from the heart fragment. After performing nucleotide (nt) sequencing of the amplicon, it was possible to demonstrate that the crab-eating fox strain exhibited high (99.8%) nt identity with the CPV-2b prototype (CPV-39 strain). Additionally, deduced amino acid (aa) sequence analysis showed the GAT codon for the aa Asp (D) at position 426 of the CPV-2 viral protein VP2, which characterizes the subtype 2b. To the best of the authors' knowledge, this report describes the first detection of CPV-2b DNA in tissue fragments from a crab-eating fox. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-23 2021-06-25T12:23:45Z 2021-06-25T12:23:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s42770-020-00389-9 Brazilian Journal Of Microbiology. New York: Springer, v. 52, n. 1, p. 449-453, 2021. 1517-8382 http://hdl.handle.net/11449/209607 10.1007/s42770-020-00389-9 WOS:000583488700001 |
url |
http://dx.doi.org/10.1007/s42770-020-00389-9 http://hdl.handle.net/11449/209607 |
identifier_str_mv |
Brazilian Journal Of Microbiology. New York: Springer, v. 52, n. 1, p. 449-453, 2021. 1517-8382 10.1007/s42770-020-00389-9 WOS:000583488700001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Brazilian Journal Of Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
449-453 |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021392715874304 |