Electrochemical magneto-immunoassay for detection of zika virus antibody in human serum
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.talanta.2023.124277 http://hdl.handle.net/11449/248376 |
Resumo: | Zika virus (ZIKV) is a flavivirus transmitted by infected Aedes genus mosquitoes. An infected person may be asymptomatic or present symptoms such as fever, arthralgia, and in pregnancy it may lead to neurological disorders in the fetus, such as microcephaly. Based on the high dissemination potential of ZIVK and its similar antigen composition to other arboviruses, new approaches for selective virus detection are urgently needed. This work reports the development of an electrochemical immunoassay for detection of anti-ZIKV antibodies, using magnetic beads functionalized with recombinant protein derived from the non-structural protein 1 (ΔNS1-ZIKV) and anti-IgG antibodies labeled with horseradish peroxidase (HRP) enzyme. The magneto-immunoassay uses disposable microfluidic devices for detection of anti-ZIKV in serum samples. A linear response was obtained for a wide concentration range from 0.01 to 9.80 × 105 pg mL−1 (r2 = 0.982), with a limit of detection of 0.48 pg mL−1. The proposed immunoassay proved to be highly efficient for the detection of anti-ZIKV antibodies in serum, offering promising perspectives for the development of fast, simple, and affordable point-of-care diagnosis devices for ZIKV. |
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Electrochemical magneto-immunoassay for detection of zika virus antibody in human serumDisposable microfluidic deviceElectrochemical immunoassayMagnetic beadsZika virus infectionZika virus (ZIKV) is a flavivirus transmitted by infected Aedes genus mosquitoes. An infected person may be asymptomatic or present symptoms such as fever, arthralgia, and in pregnancy it may lead to neurological disorders in the fetus, such as microcephaly. Based on the high dissemination potential of ZIVK and its similar antigen composition to other arboviruses, new approaches for selective virus detection are urgently needed. This work reports the development of an electrochemical immunoassay for detection of anti-ZIKV antibodies, using magnetic beads functionalized with recombinant protein derived from the non-structural protein 1 (ΔNS1-ZIKV) and anti-IgG antibodies labeled with horseradish peroxidase (HRP) enzyme. The magneto-immunoassay uses disposable microfluidic devices for detection of anti-ZIKV in serum samples. A linear response was obtained for a wide concentration range from 0.01 to 9.80 × 105 pg mL−1 (r2 = 0.982), with a limit of detection of 0.48 pg mL−1. The proposed immunoassay proved to be highly efficient for the detection of anti-ZIKV antibodies in serum, offering promising perspectives for the development of fast, simple, and affordable point-of-care diagnosis devices for ZIKV.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Department of Chemistry Federal University of São Carlos, São CarlosDepartment of Physics School of Technology and Sciences São Paulo State University, Presidente PrudenteInstitute of Biomedical Science University of São Paulo (ICB-USP)Center for Development and Innovation Butantan InstituteScientific Platform Pasteur/USPDepartment of Physics School of Technology and Sciences São Paulo State University, Presidente PrudenteCNPq: 157272/2018-4FAPESP: 2014/22401-0FAPESP: 2016/20045-7FAPESP: 2018/04332-1CAPES: Finance Code 001Universidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Butantan InstituteCastro, Karla R.Setti, Grazielle O. [UNESP]de Oliveira, Tássia R.Rodrigues-Jesus, Mônica J.Botosso, Viviane F.Perini de Araujo, Ana P.Durigon, Edson L.Ferreira, Luis C.S.Faria, Ronaldo C.2023-07-29T13:42:20Z2023-07-29T13:42:20Z2023-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.talanta.2023.124277Talanta, v. 256.0039-9140http://hdl.handle.net/11449/24837610.1016/j.talanta.2023.1242772-s2.0-85148306927Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTalantainfo:eu-repo/semantics/openAccess2023-07-29T13:42:20Zoai:repositorio.unesp.br:11449/248376Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T13:42:20Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Electrochemical magneto-immunoassay for detection of zika virus antibody in human serum |
title |
Electrochemical magneto-immunoassay for detection of zika virus antibody in human serum |
spellingShingle |
Electrochemical magneto-immunoassay for detection of zika virus antibody in human serum Castro, Karla R. Disposable microfluidic device Electrochemical immunoassay Magnetic beads Zika virus infection |
title_short |
Electrochemical magneto-immunoassay for detection of zika virus antibody in human serum |
title_full |
Electrochemical magneto-immunoassay for detection of zika virus antibody in human serum |
title_fullStr |
Electrochemical magneto-immunoassay for detection of zika virus antibody in human serum |
title_full_unstemmed |
Electrochemical magneto-immunoassay for detection of zika virus antibody in human serum |
title_sort |
Electrochemical magneto-immunoassay for detection of zika virus antibody in human serum |
author |
Castro, Karla R. |
author_facet |
Castro, Karla R. Setti, Grazielle O. [UNESP] de Oliveira, Tássia R. Rodrigues-Jesus, Mônica J. Botosso, Viviane F. Perini de Araujo, Ana P. Durigon, Edson L. Ferreira, Luis C.S. Faria, Ronaldo C. |
author_role |
author |
author2 |
Setti, Grazielle O. [UNESP] de Oliveira, Tássia R. Rodrigues-Jesus, Mônica J. Botosso, Viviane F. Perini de Araujo, Ana P. Durigon, Edson L. Ferreira, Luis C.S. Faria, Ronaldo C. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de São Carlos (UFSCar) Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) Butantan Institute |
dc.contributor.author.fl_str_mv |
Castro, Karla R. Setti, Grazielle O. [UNESP] de Oliveira, Tássia R. Rodrigues-Jesus, Mônica J. Botosso, Viviane F. Perini de Araujo, Ana P. Durigon, Edson L. Ferreira, Luis C.S. Faria, Ronaldo C. |
dc.subject.por.fl_str_mv |
Disposable microfluidic device Electrochemical immunoassay Magnetic beads Zika virus infection |
topic |
Disposable microfluidic device Electrochemical immunoassay Magnetic beads Zika virus infection |
description |
Zika virus (ZIKV) is a flavivirus transmitted by infected Aedes genus mosquitoes. An infected person may be asymptomatic or present symptoms such as fever, arthralgia, and in pregnancy it may lead to neurological disorders in the fetus, such as microcephaly. Based on the high dissemination potential of ZIVK and its similar antigen composition to other arboviruses, new approaches for selective virus detection are urgently needed. This work reports the development of an electrochemical immunoassay for detection of anti-ZIKV antibodies, using magnetic beads functionalized with recombinant protein derived from the non-structural protein 1 (ΔNS1-ZIKV) and anti-IgG antibodies labeled with horseradish peroxidase (HRP) enzyme. The magneto-immunoassay uses disposable microfluidic devices for detection of anti-ZIKV in serum samples. A linear response was obtained for a wide concentration range from 0.01 to 9.80 × 105 pg mL−1 (r2 = 0.982), with a limit of detection of 0.48 pg mL−1. The proposed immunoassay proved to be highly efficient for the detection of anti-ZIKV antibodies in serum, offering promising perspectives for the development of fast, simple, and affordable point-of-care diagnosis devices for ZIKV. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:42:20Z 2023-07-29T13:42:20Z 2023-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.talanta.2023.124277 Talanta, v. 256. 0039-9140 http://hdl.handle.net/11449/248376 10.1016/j.talanta.2023.124277 2-s2.0-85148306927 |
url |
http://dx.doi.org/10.1016/j.talanta.2023.124277 http://hdl.handle.net/11449/248376 |
identifier_str_mv |
Talanta, v. 256. 0039-9140 10.1016/j.talanta.2023.124277 2-s2.0-85148306927 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Talanta |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964652053987328 |