Coffee leaf scorch caused by a strain of Xylella fastidiosa from citrus
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1094/PDIS.2001.85.5.501 http://hdl.handle.net/11449/38427 |
Resumo: | Citrus variegated chlorosis (CVC) and coffee leaf scorch (CLS) are two economically important diseases in Brazil caused by the bacterium Xylella fastidiosa. Strains of the bacterium isolated from the two plant hosts are very closely related, and the two diseases share sharpshooter insect vectors. In order to determine if citrus strains of X. fastidiosa could infect coffee and induce CLS disease, plant inoculations were performed. Plants of coffee, Coffea arabica 'Mundo Novo', grafted on Coffea canephora var, robusta 'Apuatao 2258' were mechanically inoculated with triply cloned strains of X. fastidiosa isolated from diseased coffee and citrus. Three months postinoculation, 5 of the 10 plants inoculated with CLS-X. fastidiosa and 1 of the 10 plants inoculated with CVC-X. fastidiosa gave positive enzyme-linked immunosorbent assay (ELISA) and/or polymerase chain reaction (PCR). Eight months postinoculation, another six plants inoculated with CVC-X. fastidiosa gave positive PCR results. The two X. fastidiosa strains were isolated from the inoculated plants and showed the same characteristics as the original clones by microscopy, ELISA, and PCR. None of the plants inoculated with sterile periwinkle wilt (PW) medium as controls gave positive reactions in diagnostic tests, and none developed disease symptoms. Six months postinoculation, seven plants inoculated with CLS-X. fastidiosn and eight inoculated with CVC-X. fastidiosa began to develop characteristic CLS symptoms, including apical and marginal leaf scorch, defoliation, and reductions of internode length, leaf size, and plant height, terminal clusters of small chlorotic and deformed leaves, and lateral shoot dieback. We have demonstrated that X, fastidiosa from citrus plants is pathogenic for coffee plants. This has important consequences for the management of CLS disease and has implications for the origin of citrus variegated chlorosis disease. |
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Coffee leaf scorch caused by a strain of Xylella fastidiosa from citrusplant-pathogenic bacteriaCitrus variegated chlorosis (CVC) and coffee leaf scorch (CLS) are two economically important diseases in Brazil caused by the bacterium Xylella fastidiosa. Strains of the bacterium isolated from the two plant hosts are very closely related, and the two diseases share sharpshooter insect vectors. In order to determine if citrus strains of X. fastidiosa could infect coffee and induce CLS disease, plant inoculations were performed. Plants of coffee, Coffea arabica 'Mundo Novo', grafted on Coffea canephora var, robusta 'Apuatao 2258' were mechanically inoculated with triply cloned strains of X. fastidiosa isolated from diseased coffee and citrus. Three months postinoculation, 5 of the 10 plants inoculated with CLS-X. fastidiosa and 1 of the 10 plants inoculated with CVC-X. fastidiosa gave positive enzyme-linked immunosorbent assay (ELISA) and/or polymerase chain reaction (PCR). Eight months postinoculation, another six plants inoculated with CVC-X. fastidiosa gave positive PCR results. The two X. fastidiosa strains were isolated from the inoculated plants and showed the same characteristics as the original clones by microscopy, ELISA, and PCR. None of the plants inoculated with sterile periwinkle wilt (PW) medium as controls gave positive reactions in diagnostic tests, and none developed disease symptoms. Six months postinoculation, seven plants inoculated with CLS-X. fastidiosn and eight inoculated with CVC-X. fastidiosa began to develop characteristic CLS symptoms, including apical and marginal leaf scorch, defoliation, and reductions of internode length, leaf size, and plant height, terminal clusters of small chlorotic and deformed leaves, and lateral shoot dieback. We have demonstrated that X, fastidiosa from citrus plants is pathogenic for coffee plants. This has important consequences for the management of CLS disease and has implications for the origin of citrus variegated chlorosis disease.USDA ARS, Beltsville, MD 20705 USAFundecitrus, BR-14807040 Araraquara, SP, BrazilUNESP, Inst Chem, Araraquara, SP, BrazilUNESP, Inst Chem, Araraquara, SP, BrazilAmer Phytopathological SocUSDA ARSFundecitrusUniversidade Estadual Paulista (Unesp)Li, W. B.Pria, W. D.Teixeira, C.Miranda, V. S.Ayres, A. J.Franco, C. F.Costa, M. G.He, C. X.Costa, P. I.Hartung, J. S.2014-05-20T15:28:40Z2014-05-20T15:28:40Z2001-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article501-505http://dx.doi.org/10.1094/PDIS.2001.85.5.501Plant Disease. St Paul: Amer Phytopathological Soc, v. 85, n. 5, p. 501-505, 2001.0191-2917http://hdl.handle.net/11449/3842710.1094/PDIS.2001.85.5.501WOS:00016819290000867202237159173810000-0002-3350-8308Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlant Disease2.9410,575info:eu-repo/semantics/openAccess2021-10-23T10:26:45Zoai:repositorio.unesp.br:11449/38427Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T10:26:45Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Coffee leaf scorch caused by a strain of Xylella fastidiosa from citrus |
title |
Coffee leaf scorch caused by a strain of Xylella fastidiosa from citrus |
spellingShingle |
Coffee leaf scorch caused by a strain of Xylella fastidiosa from citrus Li, W. B. plant-pathogenic bacteria |
title_short |
Coffee leaf scorch caused by a strain of Xylella fastidiosa from citrus |
title_full |
Coffee leaf scorch caused by a strain of Xylella fastidiosa from citrus |
title_fullStr |
Coffee leaf scorch caused by a strain of Xylella fastidiosa from citrus |
title_full_unstemmed |
Coffee leaf scorch caused by a strain of Xylella fastidiosa from citrus |
title_sort |
Coffee leaf scorch caused by a strain of Xylella fastidiosa from citrus |
author |
Li, W. B. |
author_facet |
Li, W. B. Pria, W. D. Teixeira, C. Miranda, V. S. Ayres, A. J. Franco, C. F. Costa, M. G. He, C. X. Costa, P. I. Hartung, J. S. |
author_role |
author |
author2 |
Pria, W. D. Teixeira, C. Miranda, V. S. Ayres, A. J. Franco, C. F. Costa, M. G. He, C. X. Costa, P. I. Hartung, J. S. |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
USDA ARS Fundecitrus Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Li, W. B. Pria, W. D. Teixeira, C. Miranda, V. S. Ayres, A. J. Franco, C. F. Costa, M. G. He, C. X. Costa, P. I. Hartung, J. S. |
dc.subject.por.fl_str_mv |
plant-pathogenic bacteria |
topic |
plant-pathogenic bacteria |
description |
Citrus variegated chlorosis (CVC) and coffee leaf scorch (CLS) are two economically important diseases in Brazil caused by the bacterium Xylella fastidiosa. Strains of the bacterium isolated from the two plant hosts are very closely related, and the two diseases share sharpshooter insect vectors. In order to determine if citrus strains of X. fastidiosa could infect coffee and induce CLS disease, plant inoculations were performed. Plants of coffee, Coffea arabica 'Mundo Novo', grafted on Coffea canephora var, robusta 'Apuatao 2258' were mechanically inoculated with triply cloned strains of X. fastidiosa isolated from diseased coffee and citrus. Three months postinoculation, 5 of the 10 plants inoculated with CLS-X. fastidiosa and 1 of the 10 plants inoculated with CVC-X. fastidiosa gave positive enzyme-linked immunosorbent assay (ELISA) and/or polymerase chain reaction (PCR). Eight months postinoculation, another six plants inoculated with CVC-X. fastidiosa gave positive PCR results. The two X. fastidiosa strains were isolated from the inoculated plants and showed the same characteristics as the original clones by microscopy, ELISA, and PCR. None of the plants inoculated with sterile periwinkle wilt (PW) medium as controls gave positive reactions in diagnostic tests, and none developed disease symptoms. Six months postinoculation, seven plants inoculated with CLS-X. fastidiosn and eight inoculated with CVC-X. fastidiosa began to develop characteristic CLS symptoms, including apical and marginal leaf scorch, defoliation, and reductions of internode length, leaf size, and plant height, terminal clusters of small chlorotic and deformed leaves, and lateral shoot dieback. We have demonstrated that X, fastidiosa from citrus plants is pathogenic for coffee plants. This has important consequences for the management of CLS disease and has implications for the origin of citrus variegated chlorosis disease. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-05-01 2014-05-20T15:28:40Z 2014-05-20T15:28:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1094/PDIS.2001.85.5.501 Plant Disease. St Paul: Amer Phytopathological Soc, v. 85, n. 5, p. 501-505, 2001. 0191-2917 http://hdl.handle.net/11449/38427 10.1094/PDIS.2001.85.5.501 WOS:000168192900008 6720223715917381 0000-0002-3350-8308 |
url |
http://dx.doi.org/10.1094/PDIS.2001.85.5.501 http://hdl.handle.net/11449/38427 |
identifier_str_mv |
Plant Disease. St Paul: Amer Phytopathological Soc, v. 85, n. 5, p. 501-505, 2001. 0191-2917 10.1094/PDIS.2001.85.5.501 WOS:000168192900008 6720223715917381 0000-0002-3350-8308 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plant Disease 2.941 0,575 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
501-505 |
dc.publisher.none.fl_str_mv |
Amer Phytopathological Soc |
publisher.none.fl_str_mv |
Amer Phytopathological Soc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965136420601856 |