Non-mutagenic or genotoxic effects of medicinal aqueous extracts from the Agaricus blazei mushroom in V79 cells
Autor(a) principal: | |
---|---|
Data de Publicação: | 2003 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1508/cytologia.68.1 http://hdl.handle.net/11449/67209 |
Resumo: | Agaricus blazei Murill is a mushroom largely consumed due to its medicinal properties. Effects of aqueous extract from its lineage AB97/11 in 2 fruiting body development stages (closed and opened pileus) were evaluated on chinese hamster V79 cells using cytokinesis blocking micronucleus (CBMN) and comet assays. The cells were treated at 0.15% concentration of aqueous extract prepared at different temperatures: ice-cold (4°C), room temperature (21°C) and warm (60°C). The extracts were applied in mutagenicity and antimutagenicity protocols (simultaneous, pre-incubation and continuous). The results showed that the aqueous extracts of Agaricus blazei lineage AB97/11 obtained at the 3 temperatures and both development stages did not present mutagenic or antimutagenic effect in V79 cells either in CBMN or comet assay. |
id |
UNSP_2542eb9d243fc3d64f140e9038e35bc5 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/67209 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Non-mutagenic or genotoxic effects of medicinal aqueous extracts from the Agaricus blazei mushroom in V79 cellsAgaricus blazei MurillAntimutagenesisComet assayCytokinesis block micronucleus assayMethyl methanesulphonateV79 cellsAgaricusAgaricus blazeiBasidiomycotaCricetinaeCricetulus griseusAgaricus blazei Murill is a mushroom largely consumed due to its medicinal properties. Effects of aqueous extract from its lineage AB97/11 in 2 fruiting body development stages (closed and opened pileus) were evaluated on chinese hamster V79 cells using cytokinesis blocking micronucleus (CBMN) and comet assays. The cells were treated at 0.15% concentration of aqueous extract prepared at different temperatures: ice-cold (4°C), room temperature (21°C) and warm (60°C). The extracts were applied in mutagenicity and antimutagenicity protocols (simultaneous, pre-incubation and continuous). The results showed that the aqueous extracts of Agaricus blazei lineage AB97/11 obtained at the 3 temperatures and both development stages did not present mutagenic or antimutagenic effect in V79 cells either in CBMN or comet assay.Departamento de Biologia Geral Universidade Estadual de Londrina, Londrina, PRDepto. de Prod. Vegetal Faculdade de Cie. Agronomicas UNESP, Botucatu, SPDepto. de Prod. Vegetal Faculdade de Cie. Agronomicas UNESP, Botucatu, SPUniversidade Estadual de Londrina (UEL)Universidade Estadual Paulista (Unesp)Luiz, R. C.Jordão, B. Q.Da Eira, A. F. [UNESP]Ribeiro, L. R.Mantovani, M. S.2014-05-27T11:20:37Z2014-05-27T11:20:37Z2003-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-6application/pdfhttp://dx.doi.org/10.1508/cytologia.68.1Cytologia, v. 68, n. 1, p. 1-6, 2003.0011-4545http://hdl.handle.net/11449/6720910.1508/cytologia.68.12-s2.0-00373636592-s2.0-0037363659.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCytologia0.4810,233info:eu-repo/semantics/openAccess2024-04-30T15:56:29Zoai:repositorio.unesp.br:11449/67209Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:36:42.947369Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Non-mutagenic or genotoxic effects of medicinal aqueous extracts from the Agaricus blazei mushroom in V79 cells |
title |
Non-mutagenic or genotoxic effects of medicinal aqueous extracts from the Agaricus blazei mushroom in V79 cells |
spellingShingle |
Non-mutagenic or genotoxic effects of medicinal aqueous extracts from the Agaricus blazei mushroom in V79 cells Luiz, R. C. Agaricus blazei Murill Antimutagenesis Comet assay Cytokinesis block micronucleus assay Methyl methanesulphonate V79 cells Agaricus Agaricus blazei Basidiomycota Cricetinae Cricetulus griseus |
title_short |
Non-mutagenic or genotoxic effects of medicinal aqueous extracts from the Agaricus blazei mushroom in V79 cells |
title_full |
Non-mutagenic or genotoxic effects of medicinal aqueous extracts from the Agaricus blazei mushroom in V79 cells |
title_fullStr |
Non-mutagenic or genotoxic effects of medicinal aqueous extracts from the Agaricus blazei mushroom in V79 cells |
title_full_unstemmed |
Non-mutagenic or genotoxic effects of medicinal aqueous extracts from the Agaricus blazei mushroom in V79 cells |
title_sort |
Non-mutagenic or genotoxic effects of medicinal aqueous extracts from the Agaricus blazei mushroom in V79 cells |
author |
Luiz, R. C. |
author_facet |
Luiz, R. C. Jordão, B. Q. Da Eira, A. F. [UNESP] Ribeiro, L. R. Mantovani, M. S. |
author_role |
author |
author2 |
Jordão, B. Q. Da Eira, A. F. [UNESP] Ribeiro, L. R. Mantovani, M. S. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Londrina (UEL) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Luiz, R. C. Jordão, B. Q. Da Eira, A. F. [UNESP] Ribeiro, L. R. Mantovani, M. S. |
dc.subject.por.fl_str_mv |
Agaricus blazei Murill Antimutagenesis Comet assay Cytokinesis block micronucleus assay Methyl methanesulphonate V79 cells Agaricus Agaricus blazei Basidiomycota Cricetinae Cricetulus griseus |
topic |
Agaricus blazei Murill Antimutagenesis Comet assay Cytokinesis block micronucleus assay Methyl methanesulphonate V79 cells Agaricus Agaricus blazei Basidiomycota Cricetinae Cricetulus griseus |
description |
Agaricus blazei Murill is a mushroom largely consumed due to its medicinal properties. Effects of aqueous extract from its lineage AB97/11 in 2 fruiting body development stages (closed and opened pileus) were evaluated on chinese hamster V79 cells using cytokinesis blocking micronucleus (CBMN) and comet assays. The cells were treated at 0.15% concentration of aqueous extract prepared at different temperatures: ice-cold (4°C), room temperature (21°C) and warm (60°C). The extracts were applied in mutagenicity and antimutagenicity protocols (simultaneous, pre-incubation and continuous). The results showed that the aqueous extracts of Agaricus blazei lineage AB97/11 obtained at the 3 temperatures and both development stages did not present mutagenic or antimutagenic effect in V79 cells either in CBMN or comet assay. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-03-01 2014-05-27T11:20:37Z 2014-05-27T11:20:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1508/cytologia.68.1 Cytologia, v. 68, n. 1, p. 1-6, 2003. 0011-4545 http://hdl.handle.net/11449/67209 10.1508/cytologia.68.1 2-s2.0-0037363659 2-s2.0-0037363659.pdf |
url |
http://dx.doi.org/10.1508/cytologia.68.1 http://hdl.handle.net/11449/67209 |
identifier_str_mv |
Cytologia, v. 68, n. 1, p. 1-6, 2003. 0011-4545 10.1508/cytologia.68.1 2-s2.0-0037363659 2-s2.0-0037363659.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cytologia 0.481 0,233 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-6 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128835057614848 |