Non-mutagenic or genotoxic effects of medicinal aqueous extracts from the Agaricus blazei mushroom in V79 cells

Detalhes bibliográficos
Autor(a) principal: Luiz, R. C.
Data de Publicação: 2003
Outros Autores: Jordão, B. Q., Da Eira, A. F. [UNESP], Ribeiro, L. R., Mantovani, M. S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1508/cytologia.68.1
http://hdl.handle.net/11449/67209
Resumo: Agaricus blazei Murill is a mushroom largely consumed due to its medicinal properties. Effects of aqueous extract from its lineage AB97/11 in 2 fruiting body development stages (closed and opened pileus) were evaluated on chinese hamster V79 cells using cytokinesis blocking micronucleus (CBMN) and comet assays. The cells were treated at 0.15% concentration of aqueous extract prepared at different temperatures: ice-cold (4°C), room temperature (21°C) and warm (60°C). The extracts were applied in mutagenicity and antimutagenicity protocols (simultaneous, pre-incubation and continuous). The results showed that the aqueous extracts of Agaricus blazei lineage AB97/11 obtained at the 3 temperatures and both development stages did not present mutagenic or antimutagenic effect in V79 cells either in CBMN or comet assay.
id UNSP_2542eb9d243fc3d64f140e9038e35bc5
oai_identifier_str oai:repositorio.unesp.br:11449/67209
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Non-mutagenic or genotoxic effects of medicinal aqueous extracts from the Agaricus blazei mushroom in V79 cellsAgaricus blazei MurillAntimutagenesisComet assayCytokinesis block micronucleus assayMethyl methanesulphonateV79 cellsAgaricusAgaricus blazeiBasidiomycotaCricetinaeCricetulus griseusAgaricus blazei Murill is a mushroom largely consumed due to its medicinal properties. Effects of aqueous extract from its lineage AB97/11 in 2 fruiting body development stages (closed and opened pileus) were evaluated on chinese hamster V79 cells using cytokinesis blocking micronucleus (CBMN) and comet assays. The cells were treated at 0.15% concentration of aqueous extract prepared at different temperatures: ice-cold (4°C), room temperature (21°C) and warm (60°C). The extracts were applied in mutagenicity and antimutagenicity protocols (simultaneous, pre-incubation and continuous). The results showed that the aqueous extracts of Agaricus blazei lineage AB97/11 obtained at the 3 temperatures and both development stages did not present mutagenic or antimutagenic effect in V79 cells either in CBMN or comet assay.Departamento de Biologia Geral Universidade Estadual de Londrina, Londrina, PRDepto. de Prod. Vegetal Faculdade de Cie. Agronomicas UNESP, Botucatu, SPDepto. de Prod. Vegetal Faculdade de Cie. Agronomicas UNESP, Botucatu, SPUniversidade Estadual de Londrina (UEL)Universidade Estadual Paulista (Unesp)Luiz, R. C.Jordão, B. Q.Da Eira, A. F. [UNESP]Ribeiro, L. R.Mantovani, M. S.2014-05-27T11:20:37Z2014-05-27T11:20:37Z2003-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-6application/pdfhttp://dx.doi.org/10.1508/cytologia.68.1Cytologia, v. 68, n. 1, p. 1-6, 2003.0011-4545http://hdl.handle.net/11449/6720910.1508/cytologia.68.12-s2.0-00373636592-s2.0-0037363659.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCytologia0.4810,233info:eu-repo/semantics/openAccess2024-04-30T15:56:29Zoai:repositorio.unesp.br:11449/67209Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:36:42.947369Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Non-mutagenic or genotoxic effects of medicinal aqueous extracts from the Agaricus blazei mushroom in V79 cells
title Non-mutagenic or genotoxic effects of medicinal aqueous extracts from the Agaricus blazei mushroom in V79 cells
spellingShingle Non-mutagenic or genotoxic effects of medicinal aqueous extracts from the Agaricus blazei mushroom in V79 cells
Luiz, R. C.
Agaricus blazei Murill
Antimutagenesis
Comet assay
Cytokinesis block micronucleus assay
Methyl methanesulphonate
V79 cells
Agaricus
Agaricus blazei
Basidiomycota
Cricetinae
Cricetulus griseus
title_short Non-mutagenic or genotoxic effects of medicinal aqueous extracts from the Agaricus blazei mushroom in V79 cells
title_full Non-mutagenic or genotoxic effects of medicinal aqueous extracts from the Agaricus blazei mushroom in V79 cells
title_fullStr Non-mutagenic or genotoxic effects of medicinal aqueous extracts from the Agaricus blazei mushroom in V79 cells
title_full_unstemmed Non-mutagenic or genotoxic effects of medicinal aqueous extracts from the Agaricus blazei mushroom in V79 cells
title_sort Non-mutagenic or genotoxic effects of medicinal aqueous extracts from the Agaricus blazei mushroom in V79 cells
author Luiz, R. C.
author_facet Luiz, R. C.
Jordão, B. Q.
Da Eira, A. F. [UNESP]
Ribeiro, L. R.
Mantovani, M. S.
author_role author
author2 Jordão, B. Q.
Da Eira, A. F. [UNESP]
Ribeiro, L. R.
Mantovani, M. S.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Londrina (UEL)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Luiz, R. C.
Jordão, B. Q.
Da Eira, A. F. [UNESP]
Ribeiro, L. R.
Mantovani, M. S.
dc.subject.por.fl_str_mv Agaricus blazei Murill
Antimutagenesis
Comet assay
Cytokinesis block micronucleus assay
Methyl methanesulphonate
V79 cells
Agaricus
Agaricus blazei
Basidiomycota
Cricetinae
Cricetulus griseus
topic Agaricus blazei Murill
Antimutagenesis
Comet assay
Cytokinesis block micronucleus assay
Methyl methanesulphonate
V79 cells
Agaricus
Agaricus blazei
Basidiomycota
Cricetinae
Cricetulus griseus
description Agaricus blazei Murill is a mushroom largely consumed due to its medicinal properties. Effects of aqueous extract from its lineage AB97/11 in 2 fruiting body development stages (closed and opened pileus) were evaluated on chinese hamster V79 cells using cytokinesis blocking micronucleus (CBMN) and comet assays. The cells were treated at 0.15% concentration of aqueous extract prepared at different temperatures: ice-cold (4°C), room temperature (21°C) and warm (60°C). The extracts were applied in mutagenicity and antimutagenicity protocols (simultaneous, pre-incubation and continuous). The results showed that the aqueous extracts of Agaricus blazei lineage AB97/11 obtained at the 3 temperatures and both development stages did not present mutagenic or antimutagenic effect in V79 cells either in CBMN or comet assay.
publishDate 2003
dc.date.none.fl_str_mv 2003-03-01
2014-05-27T11:20:37Z
2014-05-27T11:20:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1508/cytologia.68.1
Cytologia, v. 68, n. 1, p. 1-6, 2003.
0011-4545
http://hdl.handle.net/11449/67209
10.1508/cytologia.68.1
2-s2.0-0037363659
2-s2.0-0037363659.pdf
url http://dx.doi.org/10.1508/cytologia.68.1
http://hdl.handle.net/11449/67209
identifier_str_mv Cytologia, v. 68, n. 1, p. 1-6, 2003.
0011-4545
10.1508/cytologia.68.1
2-s2.0-0037363659
2-s2.0-0037363659.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cytologia
0.481
0,233
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1-6
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128835057614848