Production of saccharogenic and dextrinogenic amylases by Rhizomucor pusillus A 13.36

• Autor(a) principal: Silva, Tony M. [UNESP]
• Data de Publicação: 2005
• Outros Autores: Attili-Angelis, Derlene [UNESP], Azevedo Carvalho, Ana Flávia [UNESP], Da Silva, Roberto [UNESP], Boscolo, Maurício [UNESP], Gomes, Eleni [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://www.ncbi.nlm.nih.gov/pubmed/16410774; http://hdl.handle.net/11449/68660
• topic: Amylase; Rhizomucor pusillus; Thermostability; amylase; calcium; copper; enzyme inhibitor; glucose; iron; maltose; maltotriose; oligosaccharide; starch; sucrose; trisaccharide; biosynthesis; cassava; enzyme stability; enzymology; metabolism; pH; Rhizomucor; temperature; time; Amylases; Calcium; Copper; Enzyme Inhibitors; Enzyme Stability; Glucose; Hydrogen-Ion Concentration; Iron; Maltose; Manihot; Oligosaccharides; Starch; Sucrose; Temperature; Time Factors; Trisaccharides; Fungi; Manihot esculenta

A newly-isolated thermophilic strain of the zygomycete fungus Rhizomucor pusillus 13.36 produced highly active dextrinogenic and saccharogenic enzymes. Cassava pulp was a good alternative substrate for amylase production. Dextrinogenic and saccharogenic amylases exhibited optimum activities at a pH of 4.0-4.5 and 5.0 respectively and at a temperature of 75°C. The enzymes were highly thermostable, with no detectable loss of saccharogenic or dextrinogenic activity after 1 h and 6 h at 60°C, respectively. The saccharogenic activity was inhibited by Ca2+ while the dextrinogenic was indifferent to this ion. Both activities were inhibited by Fe2+ and Cu2+ Hydrolysis of soluble starch by the crude enzyme yielded 66% glucose, 19.5% maltose, 7.7% maltotriose and 6.6% oligosaccharides. Copyright © 2005, The Microbiological Society of Korea.