Characterization of Luteal Blood Flow and Secretion of Progesterone in Mares Treated With Human Chorionic Gonadotropin for Ovulation Induction or During Early Diestrus

Detalhes bibliográficos
Autor(a) principal: Romano, Rafael Mide [UNESP]
Data de Publicação: 2015
Outros Autores: Ferreira, Jair Camargo [UNESP], de Siqueira Canesin, Heloisa [UNESP], Boakari, Yatta Linhares [UNESP], Ignácio, Fernanda Saules [UNESP], Novaes Filho, Luiz Fernando [UNESP], Thompson, Donald L., de Meira, Cezinande [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.jevs.2015.03.196
http://hdl.handle.net/11449/172004
Resumo: Human chorionic gonadotropin (hCG) has been used to induce ovulation and as a luteotrophic agent in cattle. However, the effect of hCG therapy on the functional status of the equine corpus luteum (CL) is unclear. This study aimed to characterize the hemodynamic and secretory function of early CL of mares treated with different doses of hCG at distinct stages of the estrous cycle. Mares were assigned to nine experimental groups (n = 6 mares/group) according to dose of hCG and time of treatment. A single injection of one of three different doses of hCG (750, 1,500, or 2,500 IU) was performed in one of three distinct stages of the estrous cycle: preovulatory follicle ≥35mm, day of ovulation (D0), or 48hours after ovulation (D2). In addition, a control group treated with NaCl 0.9% was included in the study. The end points evaluated daily from D0 to D8 were area of the CL, luteal vascularity, number of colored pixels and total pixel intensity, and concentrations of plasma progesterone (P4). No effect (. P > .1) of dose or time of treatment was observed for any end point, within each day. Luteal area did not differ throughout the days (. P > .1), whereas Doppler parameters and concentrations of plasma P4 presented a progressive increase (. P < .05) after ovulation in all groups. Secretory function and luteal hemodynamic were not affected (. P > .1) by hCG dose and time of treatment. In conclusion, hCG therapy during estrus or early diestrus, at the doses tested, did not improve P4 secretion or luteal blood flow.
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spelling Characterization of Luteal Blood Flow and Secretion of Progesterone in Mares Treated With Human Chorionic Gonadotropin for Ovulation Induction or During Early DiestrusBlood flowCorpus luteumDiestrusDoppler ultrasonographyProgesteroneHuman chorionic gonadotropin (hCG) has been used to induce ovulation and as a luteotrophic agent in cattle. However, the effect of hCG therapy on the functional status of the equine corpus luteum (CL) is unclear. This study aimed to characterize the hemodynamic and secretory function of early CL of mares treated with different doses of hCG at distinct stages of the estrous cycle. Mares were assigned to nine experimental groups (n = 6 mares/group) according to dose of hCG and time of treatment. A single injection of one of three different doses of hCG (750, 1,500, or 2,500 IU) was performed in one of three distinct stages of the estrous cycle: preovulatory follicle ≥35mm, day of ovulation (D0), or 48hours after ovulation (D2). In addition, a control group treated with NaCl 0.9% was included in the study. The end points evaluated daily from D0 to D8 were area of the CL, luteal vascularity, number of colored pixels and total pixel intensity, and concentrations of plasma progesterone (P4). No effect (. P > .1) of dose or time of treatment was observed for any end point, within each day. Luteal area did not differ throughout the days (. P > .1), whereas Doppler parameters and concentrations of plasma P4 presented a progressive increase (. P < .05) after ovulation in all groups. Secretory function and luteal hemodynamic were not affected (. P > .1) by hCG dose and time of treatment. In conclusion, hCG therapy during estrus or early diestrus, at the doses tested, did not improve P4 secretion or luteal blood flow.Department of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, UNESPSchool of Animal Sciences, Louisiana State University Agricultural CenterDepartment of Animal Reproduction and Veterinary Radiology, School of Veterinary Medicine and Animal Science, UNESPUniversidade Estadual Paulista (Unesp)School of Animal Sciences, Louisiana State University Agricultural CenterRomano, Rafael Mide [UNESP]Ferreira, Jair Camargo [UNESP]de Siqueira Canesin, Heloisa [UNESP]Boakari, Yatta Linhares [UNESP]Ignácio, Fernanda Saules [UNESP]Novaes Filho, Luiz Fernando [UNESP]Thompson, Donald L.de Meira, Cezinande [UNESP]2018-12-11T16:58:06Z2018-12-11T16:58:06Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article591-597application/pdfhttp://dx.doi.org/10.1016/j.jevs.2015.03.196Journal of Equine Veterinary Science, v. 35, n. 7, p. 591-597, 2015.0737-0806http://hdl.handle.net/11449/17200410.1016/j.jevs.2015.03.1962-s2.0-849395990352-s2.0-84939599035.pdf00429054157111990000-0002-2245-800XScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Equine Veterinary Science0,390info:eu-repo/semantics/openAccess2024-09-09T14:00:26Zoai:repositorio.unesp.br:11449/172004Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:00:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Characterization of Luteal Blood Flow and Secretion of Progesterone in Mares Treated With Human Chorionic Gonadotropin for Ovulation Induction or During Early Diestrus
title Characterization of Luteal Blood Flow and Secretion of Progesterone in Mares Treated With Human Chorionic Gonadotropin for Ovulation Induction or During Early Diestrus
spellingShingle Characterization of Luteal Blood Flow and Secretion of Progesterone in Mares Treated With Human Chorionic Gonadotropin for Ovulation Induction or During Early Diestrus
Romano, Rafael Mide [UNESP]
Blood flow
Corpus luteum
Diestrus
Doppler ultrasonography
Progesterone
title_short Characterization of Luteal Blood Flow and Secretion of Progesterone in Mares Treated With Human Chorionic Gonadotropin for Ovulation Induction or During Early Diestrus
title_full Characterization of Luteal Blood Flow and Secretion of Progesterone in Mares Treated With Human Chorionic Gonadotropin for Ovulation Induction or During Early Diestrus
title_fullStr Characterization of Luteal Blood Flow and Secretion of Progesterone in Mares Treated With Human Chorionic Gonadotropin for Ovulation Induction or During Early Diestrus
title_full_unstemmed Characterization of Luteal Blood Flow and Secretion of Progesterone in Mares Treated With Human Chorionic Gonadotropin for Ovulation Induction or During Early Diestrus
title_sort Characterization of Luteal Blood Flow and Secretion of Progesterone in Mares Treated With Human Chorionic Gonadotropin for Ovulation Induction or During Early Diestrus
author Romano, Rafael Mide [UNESP]
author_facet Romano, Rafael Mide [UNESP]
Ferreira, Jair Camargo [UNESP]
de Siqueira Canesin, Heloisa [UNESP]
Boakari, Yatta Linhares [UNESP]
Ignácio, Fernanda Saules [UNESP]
Novaes Filho, Luiz Fernando [UNESP]
Thompson, Donald L.
de Meira, Cezinande [UNESP]
author_role author
author2 Ferreira, Jair Camargo [UNESP]
de Siqueira Canesin, Heloisa [UNESP]
Boakari, Yatta Linhares [UNESP]
Ignácio, Fernanda Saules [UNESP]
Novaes Filho, Luiz Fernando [UNESP]
Thompson, Donald L.
de Meira, Cezinande [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
School of Animal Sciences, Louisiana State University Agricultural Center
dc.contributor.author.fl_str_mv Romano, Rafael Mide [UNESP]
Ferreira, Jair Camargo [UNESP]
de Siqueira Canesin, Heloisa [UNESP]
Boakari, Yatta Linhares [UNESP]
Ignácio, Fernanda Saules [UNESP]
Novaes Filho, Luiz Fernando [UNESP]
Thompson, Donald L.
de Meira, Cezinande [UNESP]
dc.subject.por.fl_str_mv Blood flow
Corpus luteum
Diestrus
Doppler ultrasonography
Progesterone
topic Blood flow
Corpus luteum
Diestrus
Doppler ultrasonography
Progesterone
description Human chorionic gonadotropin (hCG) has been used to induce ovulation and as a luteotrophic agent in cattle. However, the effect of hCG therapy on the functional status of the equine corpus luteum (CL) is unclear. This study aimed to characterize the hemodynamic and secretory function of early CL of mares treated with different doses of hCG at distinct stages of the estrous cycle. Mares were assigned to nine experimental groups (n = 6 mares/group) according to dose of hCG and time of treatment. A single injection of one of three different doses of hCG (750, 1,500, or 2,500 IU) was performed in one of three distinct stages of the estrous cycle: preovulatory follicle ≥35mm, day of ovulation (D0), or 48hours after ovulation (D2). In addition, a control group treated with NaCl 0.9% was included in the study. The end points evaluated daily from D0 to D8 were area of the CL, luteal vascularity, number of colored pixels and total pixel intensity, and concentrations of plasma progesterone (P4). No effect (. P > .1) of dose or time of treatment was observed for any end point, within each day. Luteal area did not differ throughout the days (. P > .1), whereas Doppler parameters and concentrations of plasma P4 presented a progressive increase (. P < .05) after ovulation in all groups. Secretory function and luteal hemodynamic were not affected (. P > .1) by hCG dose and time of treatment. In conclusion, hCG therapy during estrus or early diestrus, at the doses tested, did not improve P4 secretion or luteal blood flow.
publishDate 2015
dc.date.none.fl_str_mv 2015-01-01
2018-12-11T16:58:06Z
2018-12-11T16:58:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.jevs.2015.03.196
Journal of Equine Veterinary Science, v. 35, n. 7, p. 591-597, 2015.
0737-0806
http://hdl.handle.net/11449/172004
10.1016/j.jevs.2015.03.196
2-s2.0-84939599035
2-s2.0-84939599035.pdf
0042905415711199
0000-0002-2245-800X
url http://dx.doi.org/10.1016/j.jevs.2015.03.196
http://hdl.handle.net/11449/172004
identifier_str_mv Journal of Equine Veterinary Science, v. 35, n. 7, p. 591-597, 2015.
0737-0806
10.1016/j.jevs.2015.03.196
2-s2.0-84939599035
2-s2.0-84939599035.pdf
0042905415711199
0000-0002-2245-800X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Equine Veterinary Science
0,390
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 591-597
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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