The association of modified QuEChERS and DLLME to offer high analytical detectability to assess residual depletion profile of erythromycin in fish

• Autor(a) principal: Campanharo, Sarah Chagas
• Data de Publicação: 2023
• Outros Autores: da Silva, Agnaldo Fernando Baldo, Bleuzen, Anaïs, da Silva, Jonas Joaquim Mangabeira, de Freitas, Lucas Victor Pereira, Assane, Inácio Mateus [UNESP], Pilarski, Fabiana [UNESP], Paschoal, Jonas Augusto Rizzato
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1016/j.foodchem.2022.134852; http://hdl.handle.net/11449/247848
• topic: Aquaculture; DLLME; Erythromycin; Food safety; QuEChERS; Veterinary drugs residues

An analytical method for the determination of erythromycin A (ERY) residues in fish fillet was developed, optimized, and validated employing a modified QuEChERS procedure associated to DLLME technique as a preconcentration step. The obtained LOD and the LOQ were 0.1 µg kg−1 and 1 µg kg−1, respectively. The validated method provides linearity in the range of 1 to 20 µg kg−1, precision (CV < 6.3 %) and accuracy (recovery ranging from 103 to 110 %). The procedure was applied in an experimental study to evaluate the residual depletion profile of ERY in fish (Piaractus mesopotamicus) after oral administration. The treatment was carried out at a daily dose of 100 mg (kg BW)-1 of ERY, for 7 consecutive days and with an average water temperature of 30 °C. A withdrawal time of 240°-day was estimated for eliminating ERY residues at concentration levels below the maximum residue limit considered (MRL 100 µg kg−1).