Use of the paired samples (cerebrospinal fluid and serum) in immunodiagnostic of active and inactive human neurocysticercosis
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0074-02762005000400014 http://hdl.handle.net/11449/68299 |
Resumo: | Paired samples of cerebrospinal fluid (CSF) and serum of 30 patients - 10 with active, 10 with inactive neurocysticercosis (NCC), and 10 control subjects - were evaluated by enzyme-linked immunosorbent assay (ELISA) using two Taenia crassiceps metacestode extracts as antigen in order to detect IgG antibodies. In active NCC, high levels of IgG were detected (p < 0.05). The CSF samples showed 80% (CI 72-88) of reactivity in the saline extract (S) and 90% (CI 84-95) in sodium dodecyl sulphate (SDS) and the serum samples were reactive in 90% (CI 84-95) and 100% (CI 98-100) in the S and SDS antigenic extracts, respectively. The use of the paired samples of CSF and serum in active NCC showed equivalent results suggesting that the serum samples could be used as a screening in those patients whose CSF puncture is counter-indicated. |
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Repositório Institucional da UNESP |
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Use of the paired samples (cerebrospinal fluid and serum) in immunodiagnostic of active and inactive human neurocysticercosisCerebrospinal fluidEnzyme-linked immunosorbent assayNeurocysticercosisSerumTaenia crassicepsdiagnostic agenthelminth antibodyimmunoglobulin Gparasite antigenanimalbloodcase control studycerebrospinal fluidcomparative studycross reactionenzyme linked immunosorbent assayfemalehumanimmunologymaleneurocysticercosisTaenia soliumAnimalsAntibodies, HelminthAntigens, HelminthCase-Control StudiesCross ReactionsEnzyme-Linked Immunosorbent AssayFemaleHumansImmunoglobulin GMalePaired samples of cerebrospinal fluid (CSF) and serum of 30 patients - 10 with active, 10 with inactive neurocysticercosis (NCC), and 10 control subjects - were evaluated by enzyme-linked immunosorbent assay (ELISA) using two Taenia crassiceps metacestode extracts as antigen in order to detect IgG antibodies. In active NCC, high levels of IgG were detected (p < 0.05). The CSF samples showed 80% (CI 72-88) of reactivity in the saline extract (S) and 90% (CI 84-95) in sodium dodecyl sulphate (SDS) and the serum samples were reactive in 90% (CI 84-95) and 100% (CI 98-100) in the S and SDS antigenic extracts, respectively. The use of the paired samples of CSF and serum in active NCC showed equivalent results suggesting that the serum samples could be used as a screening in those patients whose CSF puncture is counter-indicated.Laboratório de Parasitologia Instituto de Ciências Biomédicas, 38400-902 Uberlândia, MGDepartamento de Clínica Médica Faculdade de Medicina Universidade Federal de Uberlândia, Av. Pará 1720, 38400-902 Uberlândia, MGDepartamento de Veterinária Higiene e Saúde Pública Universidade do Estado de São Paulo, Botucatu, SPUniversidade Federal de Uberlândia (UFU)Universidade Estadual Paulista (Unesp)Barcelos, Ivanildes Solange da CostaFerreira, Marcelo SimãoMoura, Leandro Pajuaba deBiondi, Germano Francisco [UNESP]Costa-Cruz, Julia Maria2014-05-27T11:21:22Z2014-05-27T11:21:22Z2005-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article427-429application/pdfhttp://dx.doi.org/10.1590/S0074-02762005000400014Memorias do Instituto Oswaldo Cruz, v. 100, n. 4, p. 427-429, 2005.0074-0276http://hdl.handle.net/11449/6829910.1590/S0074-02762005000400014S0074-027620050004000142-s2.0-239444970982-s2.0-23944497098.pdf8759101165889150Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMemórias do Instituto Oswaldo Cruz2.8331,172info:eu-repo/semantics/openAccess2023-10-11T06:06:48Zoai:repositorio.unesp.br:11449/68299Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:37:15.859870Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Use of the paired samples (cerebrospinal fluid and serum) in immunodiagnostic of active and inactive human neurocysticercosis |
title |
Use of the paired samples (cerebrospinal fluid and serum) in immunodiagnostic of active and inactive human neurocysticercosis |
spellingShingle |
Use of the paired samples (cerebrospinal fluid and serum) in immunodiagnostic of active and inactive human neurocysticercosis Barcelos, Ivanildes Solange da Costa Cerebrospinal fluid Enzyme-linked immunosorbent assay Neurocysticercosis Serum Taenia crassiceps diagnostic agent helminth antibody immunoglobulin G parasite antigen animal blood case control study cerebrospinal fluid comparative study cross reaction enzyme linked immunosorbent assay female human immunology male neurocysticercosis Taenia solium Animals Antibodies, Helminth Antigens, Helminth Case-Control Studies Cross Reactions Enzyme-Linked Immunosorbent Assay Female Humans Immunoglobulin G Male |
title_short |
Use of the paired samples (cerebrospinal fluid and serum) in immunodiagnostic of active and inactive human neurocysticercosis |
title_full |
Use of the paired samples (cerebrospinal fluid and serum) in immunodiagnostic of active and inactive human neurocysticercosis |
title_fullStr |
Use of the paired samples (cerebrospinal fluid and serum) in immunodiagnostic of active and inactive human neurocysticercosis |
title_full_unstemmed |
Use of the paired samples (cerebrospinal fluid and serum) in immunodiagnostic of active and inactive human neurocysticercosis |
title_sort |
Use of the paired samples (cerebrospinal fluid and serum) in immunodiagnostic of active and inactive human neurocysticercosis |
author |
Barcelos, Ivanildes Solange da Costa |
author_facet |
Barcelos, Ivanildes Solange da Costa Ferreira, Marcelo Simão Moura, Leandro Pajuaba de Biondi, Germano Francisco [UNESP] Costa-Cruz, Julia Maria |
author_role |
author |
author2 |
Ferreira, Marcelo Simão Moura, Leandro Pajuaba de Biondi, Germano Francisco [UNESP] Costa-Cruz, Julia Maria |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Uberlândia (UFU) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Barcelos, Ivanildes Solange da Costa Ferreira, Marcelo Simão Moura, Leandro Pajuaba de Biondi, Germano Francisco [UNESP] Costa-Cruz, Julia Maria |
dc.subject.por.fl_str_mv |
Cerebrospinal fluid Enzyme-linked immunosorbent assay Neurocysticercosis Serum Taenia crassiceps diagnostic agent helminth antibody immunoglobulin G parasite antigen animal blood case control study cerebrospinal fluid comparative study cross reaction enzyme linked immunosorbent assay female human immunology male neurocysticercosis Taenia solium Animals Antibodies, Helminth Antigens, Helminth Case-Control Studies Cross Reactions Enzyme-Linked Immunosorbent Assay Female Humans Immunoglobulin G Male |
topic |
Cerebrospinal fluid Enzyme-linked immunosorbent assay Neurocysticercosis Serum Taenia crassiceps diagnostic agent helminth antibody immunoglobulin G parasite antigen animal blood case control study cerebrospinal fluid comparative study cross reaction enzyme linked immunosorbent assay female human immunology male neurocysticercosis Taenia solium Animals Antibodies, Helminth Antigens, Helminth Case-Control Studies Cross Reactions Enzyme-Linked Immunosorbent Assay Female Humans Immunoglobulin G Male |
description |
Paired samples of cerebrospinal fluid (CSF) and serum of 30 patients - 10 with active, 10 with inactive neurocysticercosis (NCC), and 10 control subjects - were evaluated by enzyme-linked immunosorbent assay (ELISA) using two Taenia crassiceps metacestode extracts as antigen in order to detect IgG antibodies. In active NCC, high levels of IgG were detected (p < 0.05). The CSF samples showed 80% (CI 72-88) of reactivity in the saline extract (S) and 90% (CI 84-95) in sodium dodecyl sulphate (SDS) and the serum samples were reactive in 90% (CI 84-95) and 100% (CI 98-100) in the S and SDS antigenic extracts, respectively. The use of the paired samples of CSF and serum in active NCC showed equivalent results suggesting that the serum samples could be used as a screening in those patients whose CSF puncture is counter-indicated. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-07-01 2014-05-27T11:21:22Z 2014-05-27T11:21:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0074-02762005000400014 Memorias do Instituto Oswaldo Cruz, v. 100, n. 4, p. 427-429, 2005. 0074-0276 http://hdl.handle.net/11449/68299 10.1590/S0074-02762005000400014 S0074-02762005000400014 2-s2.0-23944497098 2-s2.0-23944497098.pdf 8759101165889150 |
url |
http://dx.doi.org/10.1590/S0074-02762005000400014 http://hdl.handle.net/11449/68299 |
identifier_str_mv |
Memorias do Instituto Oswaldo Cruz, v. 100, n. 4, p. 427-429, 2005. 0074-0276 10.1590/S0074-02762005000400014 S0074-02762005000400014 2-s2.0-23944497098 2-s2.0-23944497098.pdf 8759101165889150 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz 2.833 1,172 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
427-429 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128389119213568 |