Trypanosoma spp. In captive primates in a brazilian zoo
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.5216/rpt.v50i2.69303 http://hdl.handle.net/11449/229246 |
Resumo: | Captive animals, despite the constant care provided, are susceptible to infections from different sources. We herein report the natural trypanosome infection of 11 (28.2% positive) out of 39 non-human primates from 13 different species, in a Brazilian zoological park. Immunofluorescent antibody test (IFAT) and conventional polymerase chain reaction (cPCR) ruled out Trypanosoma cruzi, the etiological agent of Chagas disease. However, sequencing performed with positive samples employing hsp70 primers revealed similarities from 86% to 88% to diverse trypanosomes, including T. cruzi, Trypanosoma grayi, Trypanosoma lewisi, Trypanosoma rangeli and Trypanosoma vivax. We believe that the low similarity values obtained by sequencing reflect the difficulties in the molecular identification of trypanosomes, which share a large portion of their genetic material; this similarity may also preclude the diagnosis of co-infection by more than one trypanosome species. Thus, our study demonstrates the presence of diverse trypanosomes in primates, which are susceptible to infection by these parasites. Mechanical devices such as windows and bed nets, etc., are required to avoid vector insects in these environments, in addition to preventive quarantining of animals recently introduced into zoos. Therefore, investigation of the parasites in both the animals already residing in the zoo and those being introduced is of paramount importance, although no easy task. |
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Trypanosoma spp. In captive primates in a brazilian zooDiagnosisMonkeyNon-human primatesTrypanosomesCaptive animals, despite the constant care provided, are susceptible to infections from different sources. We herein report the natural trypanosome infection of 11 (28.2% positive) out of 39 non-human primates from 13 different species, in a Brazilian zoological park. Immunofluorescent antibody test (IFAT) and conventional polymerase chain reaction (cPCR) ruled out Trypanosoma cruzi, the etiological agent of Chagas disease. However, sequencing performed with positive samples employing hsp70 primers revealed similarities from 86% to 88% to diverse trypanosomes, including T. cruzi, Trypanosoma grayi, Trypanosoma lewisi, Trypanosoma rangeli and Trypanosoma vivax. We believe that the low similarity values obtained by sequencing reflect the difficulties in the molecular identification of trypanosomes, which share a large portion of their genetic material; this similarity may also preclude the diagnosis of co-infection by more than one trypanosome species. Thus, our study demonstrates the presence of diverse trypanosomes in primates, which are susceptible to infection by these parasites. Mechanical devices such as windows and bed nets, etc., are required to avoid vector insects in these environments, in addition to preventive quarantining of animals recently introduced into zoos. Therefore, investigation of the parasites in both the animals already residing in the zoo and those being introduced is of paramount importance, although no easy task.Medical School São Paulo State University (UNESP) Graduate Course in Tropical Diseases, BotucatuDepartment of Preventive Veterinary Medicine and Animal Health Veterinary Medical School University of São Paulo (USP)Department of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Science Unesp, BotucatuRegional Laboratories Center Adolfo Lutz InstituteDepartment of Pediatrics Medical School Unesp, BotucatuPaulista Agency of Agribusiness Technology (APTA), BauruMedical School São Paulo State University (UNESP) Graduate Course in Tropical Diseases, BotucatuDepartment of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine and Animal Science Unesp, BotucatuDepartment of Pediatrics Medical School Unesp, BotucatuUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Adolfo Lutz InstitutePaulista Agency of Agribusiness Technology (APTA)Dos Santos, Wesley Jose [UNESP]Guiraldi, Livia Maisa [UNESP]Dos Santos Paixão arques, Mirian [UNESP]Alves-Martin, Maria Fernanda0000-0001-9558-646X [UNESP]Sanchez, Gabriela PachecoDa Silva, Daniela Barbosa [UNESP]Richini-Pereira, Virgínia Bodelão [UNESP]Kurokawa, Cilmery Suemi [UNESP]Lucheis, Simone Baldini [UNESP]2022-04-29T08:31:24Z2022-04-29T08:31:24Z2021-08-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article121-134http://dx.doi.org/10.5216/rpt.v50i2.69303Journal of Tropical Pathology, v. 50, n. 2, p. 121-134, 2021.1980-81780301-0406http://hdl.handle.net/11449/22924610.5216/rpt.v50i2.693032-s2.0-85111625973Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Tropical Pathologyinfo:eu-repo/semantics/openAccess2022-04-29T08:31:24Zoai:repositorio.unesp.br:11449/229246Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:31:24Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Trypanosoma spp. In captive primates in a brazilian zoo |
title |
Trypanosoma spp. In captive primates in a brazilian zoo |
spellingShingle |
Trypanosoma spp. In captive primates in a brazilian zoo Dos Santos, Wesley Jose [UNESP] Diagnosis Monkey Non-human primates Trypanosomes |
title_short |
Trypanosoma spp. In captive primates in a brazilian zoo |
title_full |
Trypanosoma spp. In captive primates in a brazilian zoo |
title_fullStr |
Trypanosoma spp. In captive primates in a brazilian zoo |
title_full_unstemmed |
Trypanosoma spp. In captive primates in a brazilian zoo |
title_sort |
Trypanosoma spp. In captive primates in a brazilian zoo |
author |
Dos Santos, Wesley Jose [UNESP] |
author_facet |
Dos Santos, Wesley Jose [UNESP] Guiraldi, Livia Maisa [UNESP] Dos Santos Paixão arques, Mirian [UNESP] Alves-Martin, Maria Fernanda0000-0001-9558-646X [UNESP] Sanchez, Gabriela Pacheco Da Silva, Daniela Barbosa [UNESP] Richini-Pereira, Virgínia Bodelão [UNESP] Kurokawa, Cilmery Suemi [UNESP] Lucheis, Simone Baldini [UNESP] |
author_role |
author |
author2 |
Guiraldi, Livia Maisa [UNESP] Dos Santos Paixão arques, Mirian [UNESP] Alves-Martin, Maria Fernanda0000-0001-9558-646X [UNESP] Sanchez, Gabriela Pacheco Da Silva, Daniela Barbosa [UNESP] Richini-Pereira, Virgínia Bodelão [UNESP] Kurokawa, Cilmery Suemi [UNESP] Lucheis, Simone Baldini [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) Adolfo Lutz Institute Paulista Agency of Agribusiness Technology (APTA) |
dc.contributor.author.fl_str_mv |
Dos Santos, Wesley Jose [UNESP] Guiraldi, Livia Maisa [UNESP] Dos Santos Paixão arques, Mirian [UNESP] Alves-Martin, Maria Fernanda0000-0001-9558-646X [UNESP] Sanchez, Gabriela Pacheco Da Silva, Daniela Barbosa [UNESP] Richini-Pereira, Virgínia Bodelão [UNESP] Kurokawa, Cilmery Suemi [UNESP] Lucheis, Simone Baldini [UNESP] |
dc.subject.por.fl_str_mv |
Diagnosis Monkey Non-human primates Trypanosomes |
topic |
Diagnosis Monkey Non-human primates Trypanosomes |
description |
Captive animals, despite the constant care provided, are susceptible to infections from different sources. We herein report the natural trypanosome infection of 11 (28.2% positive) out of 39 non-human primates from 13 different species, in a Brazilian zoological park. Immunofluorescent antibody test (IFAT) and conventional polymerase chain reaction (cPCR) ruled out Trypanosoma cruzi, the etiological agent of Chagas disease. However, sequencing performed with positive samples employing hsp70 primers revealed similarities from 86% to 88% to diverse trypanosomes, including T. cruzi, Trypanosoma grayi, Trypanosoma lewisi, Trypanosoma rangeli and Trypanosoma vivax. We believe that the low similarity values obtained by sequencing reflect the difficulties in the molecular identification of trypanosomes, which share a large portion of their genetic material; this similarity may also preclude the diagnosis of co-infection by more than one trypanosome species. Thus, our study demonstrates the presence of diverse trypanosomes in primates, which are susceptible to infection by these parasites. Mechanical devices such as windows and bed nets, etc., are required to avoid vector insects in these environments, in addition to preventive quarantining of animals recently introduced into zoos. Therefore, investigation of the parasites in both the animals already residing in the zoo and those being introduced is of paramount importance, although no easy task. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-06 2022-04-29T08:31:24Z 2022-04-29T08:31:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.5216/rpt.v50i2.69303 Journal of Tropical Pathology, v. 50, n. 2, p. 121-134, 2021. 1980-8178 0301-0406 http://hdl.handle.net/11449/229246 10.5216/rpt.v50i2.69303 2-s2.0-85111625973 |
url |
http://dx.doi.org/10.5216/rpt.v50i2.69303 http://hdl.handle.net/11449/229246 |
identifier_str_mv |
Journal of Tropical Pathology, v. 50, n. 2, p. 121-134, 2021. 1980-8178 0301-0406 10.5216/rpt.v50i2.69303 2-s2.0-85111625973 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Tropical Pathology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
121-134 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803046356643741696 |