Influence of hepes on in vitro maturation of canine oocytes in different stages of the estrous cycle
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1809-6891v20e-53372 http://hdl.handle.net/11449/201676 |
Resumo: | Unlike other domestic animals, in vitro maturation (IVM) of canine oocytes still has limited success. The aim of this study was to evaluate the effect of HEPES on the in vitro maturation of dog oocytes in anestrus and diestrus. Ovaries were collected, aseptically isolated and transported refrigerated at a temperature of 4°C. Cumulus-oocyte complexe (COCs) from the two phases of estrous cycle were subjected to two treatments: medium TCM-199 with addition of 25 mM HEPES (TG) and medium without supplementation (CG). After 72 h of maturation, COCs were denuded, fixed and stained with Hoechst 33342 to assess nuclear maturation. The oocytes obtained from the anestrus and diestrus phases of the TG showed a higher frequency of oocytes in metaphase II (M-II) (p < 0.01) stage. Comparing the different reproductive status, it was observed that the oocytes obtained from the diestrus phase presented higher rates of GVBD (germinal vesicle breakdown) and M-II. Our results showed that HEPES preserves the viability and oocyte morphology essential for the acquisition of meiotic competence, increasing the M-II rates and that the oocytes obtained from the diestrus phase are better able to complete oocyte maturation. |
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Influence of hepes on in vitro maturation of canine oocytes in different stages of the estrous cycleInfluência do hepes na maturação in vitro de oócitos caninos obtidos de cadelas em diestro e anestroBiotechniquesBitchesCulture mediumEstrous cycleMeiotic competenceUnlike other domestic animals, in vitro maturation (IVM) of canine oocytes still has limited success. The aim of this study was to evaluate the effect of HEPES on the in vitro maturation of dog oocytes in anestrus and diestrus. Ovaries were collected, aseptically isolated and transported refrigerated at a temperature of 4°C. Cumulus-oocyte complexe (COCs) from the two phases of estrous cycle were subjected to two treatments: medium TCM-199 with addition of 25 mM HEPES (TG) and medium without supplementation (CG). After 72 h of maturation, COCs were denuded, fixed and stained with Hoechst 33342 to assess nuclear maturation. The oocytes obtained from the anestrus and diestrus phases of the TG showed a higher frequency of oocytes in metaphase II (M-II) (p < 0.01) stage. Comparing the different reproductive status, it was observed that the oocytes obtained from the diestrus phase presented higher rates of GVBD (germinal vesicle breakdown) and M-II. Our results showed that HEPES preserves the viability and oocyte morphology essential for the acquisition of meiotic competence, increasing the M-II rates and that the oocytes obtained from the diestrus phase are better able to complete oocyte maturation.Faculdade de Medicina Veterinária e Zootecnia - UNESPUniversidade Estadual do CearáFaculdade de Medicina Veterinária e Zootecnia - UNESPUniversidade Estadual Paulista (Unesp)Universidade Estadual do CearáPereira, Leda Maria Costa [UNESP]Bersano, Paulo Ricardo OliveiraLopes, Maria Denise [UNESP]2020-12-12T02:38:51Z2020-12-12T02:38:51Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1590/1809-6891v20e-53372Ciencia Animal Brasileira, v. 20.1809-68911518-2797http://hdl.handle.net/11449/20167610.1590/1809-6891v20e-53372S1809-689120190001003352-s2.0-85082975524S1809-68912019000100335.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporCiencia Animal Brasileirainfo:eu-repo/semantics/openAccess2024-09-09T14:06:26Zoai:repositorio.unesp.br:11449/201676Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:06:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Influence of hepes on in vitro maturation of canine oocytes in different stages of the estrous cycle Influência do hepes na maturação in vitro de oócitos caninos obtidos de cadelas em diestro e anestro |
title |
Influence of hepes on in vitro maturation of canine oocytes in different stages of the estrous cycle |
spellingShingle |
Influence of hepes on in vitro maturation of canine oocytes in different stages of the estrous cycle Pereira, Leda Maria Costa [UNESP] Biotechniques Bitches Culture medium Estrous cycle Meiotic competence |
title_short |
Influence of hepes on in vitro maturation of canine oocytes in different stages of the estrous cycle |
title_full |
Influence of hepes on in vitro maturation of canine oocytes in different stages of the estrous cycle |
title_fullStr |
Influence of hepes on in vitro maturation of canine oocytes in different stages of the estrous cycle |
title_full_unstemmed |
Influence of hepes on in vitro maturation of canine oocytes in different stages of the estrous cycle |
title_sort |
Influence of hepes on in vitro maturation of canine oocytes in different stages of the estrous cycle |
author |
Pereira, Leda Maria Costa [UNESP] |
author_facet |
Pereira, Leda Maria Costa [UNESP] Bersano, Paulo Ricardo Oliveira Lopes, Maria Denise [UNESP] |
author_role |
author |
author2 |
Bersano, Paulo Ricardo Oliveira Lopes, Maria Denise [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual do Ceará |
dc.contributor.author.fl_str_mv |
Pereira, Leda Maria Costa [UNESP] Bersano, Paulo Ricardo Oliveira Lopes, Maria Denise [UNESP] |
dc.subject.por.fl_str_mv |
Biotechniques Bitches Culture medium Estrous cycle Meiotic competence |
topic |
Biotechniques Bitches Culture medium Estrous cycle Meiotic competence |
description |
Unlike other domestic animals, in vitro maturation (IVM) of canine oocytes still has limited success. The aim of this study was to evaluate the effect of HEPES on the in vitro maturation of dog oocytes in anestrus and diestrus. Ovaries were collected, aseptically isolated and transported refrigerated at a temperature of 4°C. Cumulus-oocyte complexe (COCs) from the two phases of estrous cycle were subjected to two treatments: medium TCM-199 with addition of 25 mM HEPES (TG) and medium without supplementation (CG). After 72 h of maturation, COCs were denuded, fixed and stained with Hoechst 33342 to assess nuclear maturation. The oocytes obtained from the anestrus and diestrus phases of the TG showed a higher frequency of oocytes in metaphase II (M-II) (p < 0.01) stage. Comparing the different reproductive status, it was observed that the oocytes obtained from the diestrus phase presented higher rates of GVBD (germinal vesicle breakdown) and M-II. Our results showed that HEPES preserves the viability and oocyte morphology essential for the acquisition of meiotic competence, increasing the M-II rates and that the oocytes obtained from the diestrus phase are better able to complete oocyte maturation. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-01-01 2020-12-12T02:38:51Z 2020-12-12T02:38:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1809-6891v20e-53372 Ciencia Animal Brasileira, v. 20. 1809-6891 1518-2797 http://hdl.handle.net/11449/201676 10.1590/1809-6891v20e-53372 S1809-68912019000100335 2-s2.0-85082975524 S1809-68912019000100335.pdf |
url |
http://dx.doi.org/10.1590/1809-6891v20e-53372 http://hdl.handle.net/11449/201676 |
identifier_str_mv |
Ciencia Animal Brasileira, v. 20. 1809-6891 1518-2797 10.1590/1809-6891v20e-53372 S1809-68912019000100335 2-s2.0-85082975524 S1809-68912019000100335.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Ciencia Animal Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021353791684608 |