Mutagenicity and antimutagenicity of (-)-hinokinin a trypanosomicidal compound measured by Salmonella microsome and comet assays

Detalhes bibliográficos
Autor(a) principal: Resende, Flavia Aparecida [UNESP]
Data de Publicação: 2012
Outros Autores: Barbosa, Lilian Cristina, Tavares, Denise Crispim, de Camargo, Mariana Santoro [UNESP], de Souza Rezende, Karen Cristina, de Andrade e Silva, Marcio Luis, Varanda, Eliana Aparecida [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1186/1472-6882-12-203
http://hdl.handle.net/11449/7506
Resumo: Background: The dibenzylbutyrolactone lignan (-)-hinokinin (HK) was derived by partial synthesis from (-)- cubebin, isolated from the dry seeds of the pepper, Piper cubeba. Considering the good trypanosomicidal activity of HK and recalling that natural products are promising starting points for the discovery of novel potentially therapeutic agents, the aim of the present study was to investigate the (anti) mutagenic/genotoxic activities of HK.Methods: The mutagenic/genotoxic activities were evaluated by the Ames test on Salmonella typhimurium strains TA98, TA97a, TA100 and TA102, and the comet assay, so as to assess the safe use of HK in the treatment of Chagas' disease. The antimutagenic /antigenotoxic potential of HK were also tested against the mutagenicity of a variety of direct and indirect acting mutagens, such as 4- nitro-o-phenylenediamine (NOPD), sodium azide (SA), mitomycin C (MMC), benzo[a] pyrene (B[a] P), aflatoxin B-1 (AFB(1)), 2-aminoanthracene (2-AA) and 2-aminofluorene (2-AF), by the Ames test, and doxorubicin (DXR) by the comet assay.Results: The mutagenicity/genotoxicity tests showed that HK did not induce any increase in the number of revertants or extent of DNA damage, demonstrating the absence of mutagenic and genotoxic activities. on the other hand, the results on the antimutagenic potential of HK showed a strong inhibitory effect against some direct and indirect-acting mutagens.Conclusions: Regarding the use of HK as an antichagasic drug, the absence of mutagenic effects in animal cell and bacterial systems is encouraging. In addition, HK may be a new potential antigenotoxic /antimutagenic agent from natural sources. However, the protective activity of HK is not general and varies with the type of DNA damage-inducing agent used.
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spelling Mutagenicity and antimutagenicity of (-)-hinokinin a trypanosomicidal compound measured by Salmonella microsome and comet assaysHinokininAmes testComet assayMutagenicityAntimutagenicityBackground: The dibenzylbutyrolactone lignan (-)-hinokinin (HK) was derived by partial synthesis from (-)- cubebin, isolated from the dry seeds of the pepper, Piper cubeba. Considering the good trypanosomicidal activity of HK and recalling that natural products are promising starting points for the discovery of novel potentially therapeutic agents, the aim of the present study was to investigate the (anti) mutagenic/genotoxic activities of HK.Methods: The mutagenic/genotoxic activities were evaluated by the Ames test on Salmonella typhimurium strains TA98, TA97a, TA100 and TA102, and the comet assay, so as to assess the safe use of HK in the treatment of Chagas' disease. The antimutagenic /antigenotoxic potential of HK were also tested against the mutagenicity of a variety of direct and indirect acting mutagens, such as 4- nitro-o-phenylenediamine (NOPD), sodium azide (SA), mitomycin C (MMC), benzo[a] pyrene (B[a] P), aflatoxin B-1 (AFB(1)), 2-aminoanthracene (2-AA) and 2-aminofluorene (2-AF), by the Ames test, and doxorubicin (DXR) by the comet assay.Results: The mutagenicity/genotoxicity tests showed that HK did not induce any increase in the number of revertants or extent of DNA damage, demonstrating the absence of mutagenic and genotoxic activities. on the other hand, the results on the antimutagenic potential of HK showed a strong inhibitory effect against some direct and indirect-acting mutagens.Conclusions: Regarding the use of HK as an antichagasic drug, the absence of mutagenic effects in animal cell and bacterial systems is encouraging. In addition, HK may be a new potential antigenotoxic /antimutagenic agent from natural sources. However, the protective activity of HK is not general and varies with the type of DNA damage-inducing agent used.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)UNESP Univ Estadual Paulista Julio Mesquita Filho, Fac Ciencias Farmaceut Araraquara, Dept Ciencias Biol, BR-14801902 Araraquara, SP, BrazilUniv Franca, BR-14404600 Franca, SP, BrazilUNESP Univ Estadual Paulista Julio Mesquita Filho, Fac Ciencias Farmaceut Araraquara, Dept Ciencias Biol, BR-14801902 Araraquara, SP, BrazilBiomed Central Ltd.Universidade Estadual Paulista (Unesp)Univ FrancaResende, Flavia Aparecida [UNESP]Barbosa, Lilian CristinaTavares, Denise Crispimde Camargo, Mariana Santoro [UNESP]de Souza Rezende, Karen Cristinade Andrade e Silva, Marcio LuisVaranda, Eliana Aparecida [UNESP]2014-05-20T13:24:19Z2014-05-20T13:24:19Z2012-10-31info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://dx.doi.org/10.1186/1472-6882-12-203Bmc Complementary and Alternative Medicine. London: Biomed Central Ltd., v. 12, p. 10, 2012.1472-6882http://hdl.handle.net/11449/750610.1186/1472-6882-12-203WOS:000313500600001WOS000313500600001.pdf7501930236496670Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Complementary and Alternative Medicine2.1090,858info:eu-repo/semantics/openAccess2024-06-24T13:07:39Zoai:repositorio.unesp.br:11449/7506Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:28:02.548697Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Mutagenicity and antimutagenicity of (-)-hinokinin a trypanosomicidal compound measured by Salmonella microsome and comet assays
title Mutagenicity and antimutagenicity of (-)-hinokinin a trypanosomicidal compound measured by Salmonella microsome and comet assays
spellingShingle Mutagenicity and antimutagenicity of (-)-hinokinin a trypanosomicidal compound measured by Salmonella microsome and comet assays
Resende, Flavia Aparecida [UNESP]
Hinokinin
Ames test
Comet assay
Mutagenicity
Antimutagenicity
title_short Mutagenicity and antimutagenicity of (-)-hinokinin a trypanosomicidal compound measured by Salmonella microsome and comet assays
title_full Mutagenicity and antimutagenicity of (-)-hinokinin a trypanosomicidal compound measured by Salmonella microsome and comet assays
title_fullStr Mutagenicity and antimutagenicity of (-)-hinokinin a trypanosomicidal compound measured by Salmonella microsome and comet assays
title_full_unstemmed Mutagenicity and antimutagenicity of (-)-hinokinin a trypanosomicidal compound measured by Salmonella microsome and comet assays
title_sort Mutagenicity and antimutagenicity of (-)-hinokinin a trypanosomicidal compound measured by Salmonella microsome and comet assays
author Resende, Flavia Aparecida [UNESP]
author_facet Resende, Flavia Aparecida [UNESP]
Barbosa, Lilian Cristina
Tavares, Denise Crispim
de Camargo, Mariana Santoro [UNESP]
de Souza Rezende, Karen Cristina
de Andrade e Silva, Marcio Luis
Varanda, Eliana Aparecida [UNESP]
author_role author
author2 Barbosa, Lilian Cristina
Tavares, Denise Crispim
de Camargo, Mariana Santoro [UNESP]
de Souza Rezende, Karen Cristina
de Andrade e Silva, Marcio Luis
Varanda, Eliana Aparecida [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Univ Franca
dc.contributor.author.fl_str_mv Resende, Flavia Aparecida [UNESP]
Barbosa, Lilian Cristina
Tavares, Denise Crispim
de Camargo, Mariana Santoro [UNESP]
de Souza Rezende, Karen Cristina
de Andrade e Silva, Marcio Luis
Varanda, Eliana Aparecida [UNESP]
dc.subject.por.fl_str_mv Hinokinin
Ames test
Comet assay
Mutagenicity
Antimutagenicity
topic Hinokinin
Ames test
Comet assay
Mutagenicity
Antimutagenicity
description Background: The dibenzylbutyrolactone lignan (-)-hinokinin (HK) was derived by partial synthesis from (-)- cubebin, isolated from the dry seeds of the pepper, Piper cubeba. Considering the good trypanosomicidal activity of HK and recalling that natural products are promising starting points for the discovery of novel potentially therapeutic agents, the aim of the present study was to investigate the (anti) mutagenic/genotoxic activities of HK.Methods: The mutagenic/genotoxic activities were evaluated by the Ames test on Salmonella typhimurium strains TA98, TA97a, TA100 and TA102, and the comet assay, so as to assess the safe use of HK in the treatment of Chagas' disease. The antimutagenic /antigenotoxic potential of HK were also tested against the mutagenicity of a variety of direct and indirect acting mutagens, such as 4- nitro-o-phenylenediamine (NOPD), sodium azide (SA), mitomycin C (MMC), benzo[a] pyrene (B[a] P), aflatoxin B-1 (AFB(1)), 2-aminoanthracene (2-AA) and 2-aminofluorene (2-AF), by the Ames test, and doxorubicin (DXR) by the comet assay.Results: The mutagenicity/genotoxicity tests showed that HK did not induce any increase in the number of revertants or extent of DNA damage, demonstrating the absence of mutagenic and genotoxic activities. on the other hand, the results on the antimutagenic potential of HK showed a strong inhibitory effect against some direct and indirect-acting mutagens.Conclusions: Regarding the use of HK as an antichagasic drug, the absence of mutagenic effects in animal cell and bacterial systems is encouraging. In addition, HK may be a new potential antigenotoxic /antimutagenic agent from natural sources. However, the protective activity of HK is not general and varies with the type of DNA damage-inducing agent used.
publishDate 2012
dc.date.none.fl_str_mv 2012-10-31
2014-05-20T13:24:19Z
2014-05-20T13:24:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/1472-6882-12-203
Bmc Complementary and Alternative Medicine. London: Biomed Central Ltd., v. 12, p. 10, 2012.
1472-6882
http://hdl.handle.net/11449/7506
10.1186/1472-6882-12-203
WOS:000313500600001
WOS000313500600001.pdf
7501930236496670
url http://dx.doi.org/10.1186/1472-6882-12-203
http://hdl.handle.net/11449/7506
identifier_str_mv Bmc Complementary and Alternative Medicine. London: Biomed Central Ltd., v. 12, p. 10, 2012.
1472-6882
10.1186/1472-6882-12-203
WOS:000313500600001
WOS000313500600001.pdf
7501930236496670
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv BMC Complementary and Alternative Medicine
2.109
0,858
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10
application/pdf
dc.publisher.none.fl_str_mv Biomed Central Ltd.
publisher.none.fl_str_mv Biomed Central Ltd.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
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collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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