Mutagenicity and antimutagenicity of (-)-hinokinin a trypanosomicidal compound measured by Salmonella microsome and comet assays
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1472-6882-12-203 http://hdl.handle.net/11449/7506 |
Resumo: | Background: The dibenzylbutyrolactone lignan (-)-hinokinin (HK) was derived by partial synthesis from (-)- cubebin, isolated from the dry seeds of the pepper, Piper cubeba. Considering the good trypanosomicidal activity of HK and recalling that natural products are promising starting points for the discovery of novel potentially therapeutic agents, the aim of the present study was to investigate the (anti) mutagenic/genotoxic activities of HK.Methods: The mutagenic/genotoxic activities were evaluated by the Ames test on Salmonella typhimurium strains TA98, TA97a, TA100 and TA102, and the comet assay, so as to assess the safe use of HK in the treatment of Chagas' disease. The antimutagenic /antigenotoxic potential of HK were also tested against the mutagenicity of a variety of direct and indirect acting mutagens, such as 4- nitro-o-phenylenediamine (NOPD), sodium azide (SA), mitomycin C (MMC), benzo[a] pyrene (B[a] P), aflatoxin B-1 (AFB(1)), 2-aminoanthracene (2-AA) and 2-aminofluorene (2-AF), by the Ames test, and doxorubicin (DXR) by the comet assay.Results: The mutagenicity/genotoxicity tests showed that HK did not induce any increase in the number of revertants or extent of DNA damage, demonstrating the absence of mutagenic and genotoxic activities. on the other hand, the results on the antimutagenic potential of HK showed a strong inhibitory effect against some direct and indirect-acting mutagens.Conclusions: Regarding the use of HK as an antichagasic drug, the absence of mutagenic effects in animal cell and bacterial systems is encouraging. In addition, HK may be a new potential antigenotoxic /antimutagenic agent from natural sources. However, the protective activity of HK is not general and varies with the type of DNA damage-inducing agent used. |
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Mutagenicity and antimutagenicity of (-)-hinokinin a trypanosomicidal compound measured by Salmonella microsome and comet assaysHinokininAmes testComet assayMutagenicityAntimutagenicityBackground: The dibenzylbutyrolactone lignan (-)-hinokinin (HK) was derived by partial synthesis from (-)- cubebin, isolated from the dry seeds of the pepper, Piper cubeba. Considering the good trypanosomicidal activity of HK and recalling that natural products are promising starting points for the discovery of novel potentially therapeutic agents, the aim of the present study was to investigate the (anti) mutagenic/genotoxic activities of HK.Methods: The mutagenic/genotoxic activities were evaluated by the Ames test on Salmonella typhimurium strains TA98, TA97a, TA100 and TA102, and the comet assay, so as to assess the safe use of HK in the treatment of Chagas' disease. The antimutagenic /antigenotoxic potential of HK were also tested against the mutagenicity of a variety of direct and indirect acting mutagens, such as 4- nitro-o-phenylenediamine (NOPD), sodium azide (SA), mitomycin C (MMC), benzo[a] pyrene (B[a] P), aflatoxin B-1 (AFB(1)), 2-aminoanthracene (2-AA) and 2-aminofluorene (2-AF), by the Ames test, and doxorubicin (DXR) by the comet assay.Results: The mutagenicity/genotoxicity tests showed that HK did not induce any increase in the number of revertants or extent of DNA damage, demonstrating the absence of mutagenic and genotoxic activities. on the other hand, the results on the antimutagenic potential of HK showed a strong inhibitory effect against some direct and indirect-acting mutagens.Conclusions: Regarding the use of HK as an antichagasic drug, the absence of mutagenic effects in animal cell and bacterial systems is encouraging. In addition, HK may be a new potential antigenotoxic /antimutagenic agent from natural sources. However, the protective activity of HK is not general and varies with the type of DNA damage-inducing agent used.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)UNESP Univ Estadual Paulista Julio Mesquita Filho, Fac Ciencias Farmaceut Araraquara, Dept Ciencias Biol, BR-14801902 Araraquara, SP, BrazilUniv Franca, BR-14404600 Franca, SP, BrazilUNESP Univ Estadual Paulista Julio Mesquita Filho, Fac Ciencias Farmaceut Araraquara, Dept Ciencias Biol, BR-14801902 Araraquara, SP, BrazilBiomed Central Ltd.Universidade Estadual Paulista (Unesp)Univ FrancaResende, Flavia Aparecida [UNESP]Barbosa, Lilian CristinaTavares, Denise Crispimde Camargo, Mariana Santoro [UNESP]de Souza Rezende, Karen Cristinade Andrade e Silva, Marcio LuisVaranda, Eliana Aparecida [UNESP]2014-05-20T13:24:19Z2014-05-20T13:24:19Z2012-10-31info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://dx.doi.org/10.1186/1472-6882-12-203Bmc Complementary and Alternative Medicine. London: Biomed Central Ltd., v. 12, p. 10, 2012.1472-6882http://hdl.handle.net/11449/750610.1186/1472-6882-12-203WOS:000313500600001WOS000313500600001.pdf7501930236496670Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Complementary and Alternative Medicine2.1090,858info:eu-repo/semantics/openAccess2024-06-24T13:07:39Zoai:repositorio.unesp.br:11449/7506Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:28:02.548697Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mutagenicity and antimutagenicity of (-)-hinokinin a trypanosomicidal compound measured by Salmonella microsome and comet assays |
title |
Mutagenicity and antimutagenicity of (-)-hinokinin a trypanosomicidal compound measured by Salmonella microsome and comet assays |
spellingShingle |
Mutagenicity and antimutagenicity of (-)-hinokinin a trypanosomicidal compound measured by Salmonella microsome and comet assays Resende, Flavia Aparecida [UNESP] Hinokinin Ames test Comet assay Mutagenicity Antimutagenicity |
title_short |
Mutagenicity and antimutagenicity of (-)-hinokinin a trypanosomicidal compound measured by Salmonella microsome and comet assays |
title_full |
Mutagenicity and antimutagenicity of (-)-hinokinin a trypanosomicidal compound measured by Salmonella microsome and comet assays |
title_fullStr |
Mutagenicity and antimutagenicity of (-)-hinokinin a trypanosomicidal compound measured by Salmonella microsome and comet assays |
title_full_unstemmed |
Mutagenicity and antimutagenicity of (-)-hinokinin a trypanosomicidal compound measured by Salmonella microsome and comet assays |
title_sort |
Mutagenicity and antimutagenicity of (-)-hinokinin a trypanosomicidal compound measured by Salmonella microsome and comet assays |
author |
Resende, Flavia Aparecida [UNESP] |
author_facet |
Resende, Flavia Aparecida [UNESP] Barbosa, Lilian Cristina Tavares, Denise Crispim de Camargo, Mariana Santoro [UNESP] de Souza Rezende, Karen Cristina de Andrade e Silva, Marcio Luis Varanda, Eliana Aparecida [UNESP] |
author_role |
author |
author2 |
Barbosa, Lilian Cristina Tavares, Denise Crispim de Camargo, Mariana Santoro [UNESP] de Souza Rezende, Karen Cristina de Andrade e Silva, Marcio Luis Varanda, Eliana Aparecida [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Franca |
dc.contributor.author.fl_str_mv |
Resende, Flavia Aparecida [UNESP] Barbosa, Lilian Cristina Tavares, Denise Crispim de Camargo, Mariana Santoro [UNESP] de Souza Rezende, Karen Cristina de Andrade e Silva, Marcio Luis Varanda, Eliana Aparecida [UNESP] |
dc.subject.por.fl_str_mv |
Hinokinin Ames test Comet assay Mutagenicity Antimutagenicity |
topic |
Hinokinin Ames test Comet assay Mutagenicity Antimutagenicity |
description |
Background: The dibenzylbutyrolactone lignan (-)-hinokinin (HK) was derived by partial synthesis from (-)- cubebin, isolated from the dry seeds of the pepper, Piper cubeba. Considering the good trypanosomicidal activity of HK and recalling that natural products are promising starting points for the discovery of novel potentially therapeutic agents, the aim of the present study was to investigate the (anti) mutagenic/genotoxic activities of HK.Methods: The mutagenic/genotoxic activities were evaluated by the Ames test on Salmonella typhimurium strains TA98, TA97a, TA100 and TA102, and the comet assay, so as to assess the safe use of HK in the treatment of Chagas' disease. The antimutagenic /antigenotoxic potential of HK were also tested against the mutagenicity of a variety of direct and indirect acting mutagens, such as 4- nitro-o-phenylenediamine (NOPD), sodium azide (SA), mitomycin C (MMC), benzo[a] pyrene (B[a] P), aflatoxin B-1 (AFB(1)), 2-aminoanthracene (2-AA) and 2-aminofluorene (2-AF), by the Ames test, and doxorubicin (DXR) by the comet assay.Results: The mutagenicity/genotoxicity tests showed that HK did not induce any increase in the number of revertants or extent of DNA damage, demonstrating the absence of mutagenic and genotoxic activities. on the other hand, the results on the antimutagenic potential of HK showed a strong inhibitory effect against some direct and indirect-acting mutagens.Conclusions: Regarding the use of HK as an antichagasic drug, the absence of mutagenic effects in animal cell and bacterial systems is encouraging. In addition, HK may be a new potential antigenotoxic /antimutagenic agent from natural sources. However, the protective activity of HK is not general and varies with the type of DNA damage-inducing agent used. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-10-31 2014-05-20T13:24:19Z 2014-05-20T13:24:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1472-6882-12-203 Bmc Complementary and Alternative Medicine. London: Biomed Central Ltd., v. 12, p. 10, 2012. 1472-6882 http://hdl.handle.net/11449/7506 10.1186/1472-6882-12-203 WOS:000313500600001 WOS000313500600001.pdf 7501930236496670 |
url |
http://dx.doi.org/10.1186/1472-6882-12-203 http://hdl.handle.net/11449/7506 |
identifier_str_mv |
Bmc Complementary and Alternative Medicine. London: Biomed Central Ltd., v. 12, p. 10, 2012. 1472-6882 10.1186/1472-6882-12-203 WOS:000313500600001 WOS000313500600001.pdf 7501930236496670 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Complementary and Alternative Medicine 2.109 0,858 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd. |
publisher.none.fl_str_mv |
Biomed Central Ltd. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128937124954112 |