Sex steroid receptors and apoptosis-related proteins are differentially expressed in polycystic ovaries of adult dogs
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.tice.2015.12.003 http://hdl.handle.net/11449/177688 |
Resumo: | In Polycystic Ovaries (PCOs), the dynamics of sex hormone receptors and follicle-related apoptotic signaling remain unknown. In this study, we investigated the expression of androgen receptors (AR), estrogen receptors (ERα and ERβ), and apoptosis-related molecules (BAX, active caspase-3, Bcl-2 and Survivin) on different follicular stages of PCOs in adult dogs. Clinical evidences of high estradiol and testosterone levels, persistent estrus and vaginal discharge were observed. Inhibin B immunolabeling was increased in primary and 2 to 5-mm follicles, and a marked epithelial hyperplasia was common in the ovarian surface. Ovarian epithelia and primary follicles showed low expression of AR, ERα, and ERβ, whereas a moderate immunoexpression of AR was found in theca cells of secondary follicles and cysts. In PCOs, growing follicles displayed ERα expression, and secondary follicles exhibited higher ERβ expression. In addition, while few ERα-positive cells were found in the cysts, ERβ was moderately expressed in growing follicles and cysts. BAX was upregulated in the ovarian epithelium, primary follicles, and in the wall of follicular cysts. Active caspase-3 was significantly downregulated in the epithelium, primary follicles, and follicular cysts, whereas growing follicles had a strong immunoexpression in the granulosa cells. Bcl-2 and survivin were increased in the epithelium and primary follicles, and only survivin was upregulated in secondary and growing follicles. While Bcl-2 had a diffuse immunexpression in the follicular cysts, survivin was overexpressed by these cells. We concluded that sex steroid receptors and apoptotic proteins are differentially expressed in the follicles of adult dogs with PCOs. |
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Sex steroid receptors and apoptosis-related proteins are differentially expressed in polycystic ovaries of adult dogsApoptosisARDogERPolycystic ovarySex steroid receptorsIn Polycystic Ovaries (PCOs), the dynamics of sex hormone receptors and follicle-related apoptotic signaling remain unknown. In this study, we investigated the expression of androgen receptors (AR), estrogen receptors (ERα and ERβ), and apoptosis-related molecules (BAX, active caspase-3, Bcl-2 and Survivin) on different follicular stages of PCOs in adult dogs. Clinical evidences of high estradiol and testosterone levels, persistent estrus and vaginal discharge were observed. Inhibin B immunolabeling was increased in primary and 2 to 5-mm follicles, and a marked epithelial hyperplasia was common in the ovarian surface. Ovarian epithelia and primary follicles showed low expression of AR, ERα, and ERβ, whereas a moderate immunoexpression of AR was found in theca cells of secondary follicles and cysts. In PCOs, growing follicles displayed ERα expression, and secondary follicles exhibited higher ERβ expression. In addition, while few ERα-positive cells were found in the cysts, ERβ was moderately expressed in growing follicles and cysts. BAX was upregulated in the ovarian epithelium, primary follicles, and in the wall of follicular cysts. Active caspase-3 was significantly downregulated in the epithelium, primary follicles, and follicular cysts, whereas growing follicles had a strong immunoexpression in the granulosa cells. Bcl-2 and survivin were increased in the epithelium and primary follicles, and only survivin was upregulated in secondary and growing follicles. While Bcl-2 had a diffuse immunexpression in the follicular cysts, survivin was overexpressed by these cells. We concluded that sex steroid receptors and apoptotic proteins are differentially expressed in the follicles of adult dogs with PCOs.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Anatomy Biosciences Institute UNESP – Univ. Estadual PaulistaDepartment of Veterinary Faculty of Veterinary Medicine and Zootechny UNESP – Univ. Estadual PaulistaDepartment of Anatomy Biosciences Institute UNESP – Univ. Estadual PaulistaDepartment of Veterinary Faculty of Veterinary Medicine and Zootechny UNESP – Univ. Estadual PaulistaUniversidade Estadual Paulista (Unesp)Chuffa, Luiz Gustavo de Almeida [UNESP]Lupi Júnior, Luiz Antonio [UNESP]da Maia Lima, Alfredo Feio [UNESP]2018-12-11T17:26:39Z2018-12-11T17:26:39Z2015-09-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10-17application/pdfhttp://dx.doi.org/10.1016/j.tice.2015.12.003Tissue and Cell, v. 48, n. 1, p. 10-17, 2015.1532-30720040-8166http://hdl.handle.net/11449/17768810.1016/j.tice.2015.12.0032-s2.0-849529009632-s2.0-84952900963.pdf5121319676503034Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTissue and Cell0,410info:eu-repo/semantics/openAccess2024-01-05T06:28:03Zoai:repositorio.unesp.br:11449/177688Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-05-23T19:54:01.882725Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Sex steroid receptors and apoptosis-related proteins are differentially expressed in polycystic ovaries of adult dogs |
title |
Sex steroid receptors and apoptosis-related proteins are differentially expressed in polycystic ovaries of adult dogs |
spellingShingle |
Sex steroid receptors and apoptosis-related proteins are differentially expressed in polycystic ovaries of adult dogs Chuffa, Luiz Gustavo de Almeida [UNESP] Apoptosis AR Dog ER Polycystic ovary Sex steroid receptors |
title_short |
Sex steroid receptors and apoptosis-related proteins are differentially expressed in polycystic ovaries of adult dogs |
title_full |
Sex steroid receptors and apoptosis-related proteins are differentially expressed in polycystic ovaries of adult dogs |
title_fullStr |
Sex steroid receptors and apoptosis-related proteins are differentially expressed in polycystic ovaries of adult dogs |
title_full_unstemmed |
Sex steroid receptors and apoptosis-related proteins are differentially expressed in polycystic ovaries of adult dogs |
title_sort |
Sex steroid receptors and apoptosis-related proteins are differentially expressed in polycystic ovaries of adult dogs |
author |
Chuffa, Luiz Gustavo de Almeida [UNESP] |
author_facet |
Chuffa, Luiz Gustavo de Almeida [UNESP] Lupi Júnior, Luiz Antonio [UNESP] da Maia Lima, Alfredo Feio [UNESP] |
author_role |
author |
author2 |
Lupi Júnior, Luiz Antonio [UNESP] da Maia Lima, Alfredo Feio [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Chuffa, Luiz Gustavo de Almeida [UNESP] Lupi Júnior, Luiz Antonio [UNESP] da Maia Lima, Alfredo Feio [UNESP] |
dc.subject.por.fl_str_mv |
Apoptosis AR Dog ER Polycystic ovary Sex steroid receptors |
topic |
Apoptosis AR Dog ER Polycystic ovary Sex steroid receptors |
description |
In Polycystic Ovaries (PCOs), the dynamics of sex hormone receptors and follicle-related apoptotic signaling remain unknown. In this study, we investigated the expression of androgen receptors (AR), estrogen receptors (ERα and ERβ), and apoptosis-related molecules (BAX, active caspase-3, Bcl-2 and Survivin) on different follicular stages of PCOs in adult dogs. Clinical evidences of high estradiol and testosterone levels, persistent estrus and vaginal discharge were observed. Inhibin B immunolabeling was increased in primary and 2 to 5-mm follicles, and a marked epithelial hyperplasia was common in the ovarian surface. Ovarian epithelia and primary follicles showed low expression of AR, ERα, and ERβ, whereas a moderate immunoexpression of AR was found in theca cells of secondary follicles and cysts. In PCOs, growing follicles displayed ERα expression, and secondary follicles exhibited higher ERβ expression. In addition, while few ERα-positive cells were found in the cysts, ERβ was moderately expressed in growing follicles and cysts. BAX was upregulated in the ovarian epithelium, primary follicles, and in the wall of follicular cysts. Active caspase-3 was significantly downregulated in the epithelium, primary follicles, and follicular cysts, whereas growing follicles had a strong immunoexpression in the granulosa cells. Bcl-2 and survivin were increased in the epithelium and primary follicles, and only survivin was upregulated in secondary and growing follicles. While Bcl-2 had a diffuse immunexpression in the follicular cysts, survivin was overexpressed by these cells. We concluded that sex steroid receptors and apoptotic proteins are differentially expressed in the follicles of adult dogs with PCOs. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-09-10 2018-12-11T17:26:39Z 2018-12-11T17:26:39Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.tice.2015.12.003 Tissue and Cell, v. 48, n. 1, p. 10-17, 2015. 1532-3072 0040-8166 http://hdl.handle.net/11449/177688 10.1016/j.tice.2015.12.003 2-s2.0-84952900963 2-s2.0-84952900963.pdf 5121319676503034 |
url |
http://dx.doi.org/10.1016/j.tice.2015.12.003 http://hdl.handle.net/11449/177688 |
identifier_str_mv |
Tissue and Cell, v. 48, n. 1, p. 10-17, 2015. 1532-3072 0040-8166 10.1016/j.tice.2015.12.003 2-s2.0-84952900963 2-s2.0-84952900963.pdf 5121319676503034 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Tissue and Cell 0,410 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10-17 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803045636759617536 |