Protective effects of purple carrot extract (Daucus carota) against rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s12032-018-1114-7 http://hdl.handle.net/11449/176034 |
Resumo: | The aim of this study was to evaluate the chemopreventive potential of purple carrot extract following rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide (4NQO). For this purpose, histopathological analysis, proliferative status, antioxidant activity and inflammatory status were investigated in this setting. A total of 20 male rats were distributed into four groups as follows (n = 5 per group): Group 1—free access to water and commercial diet for 12 weeks; Group 2—received 4NQO at 50 ppm dose in drinking water daily and commercial diet for 12 weeks; Group 3—free access to water and received diet supplemented with purple carrot extract (0.1 g/kg) for 12 weeks; and Group 4—received 4NQO at 50 ppm dose in drinking water daily and diet supplemented with purple carrot extract (0.1 g/kg) for 12 weeks. Histopathological analysis revealed that animals treated with purple carrot extract reduced the oral lesions such as dysplasia and squamous cell carcinoma. Animals with oral pre-neoplastic lesions and treated with purple carrot extract decreased ki-67 and 8-OHdG immunoexpression. Moreover, pNFκBp50 and MyD88 protein expressions were decreased after purple carrot treatment associated or not with 4NQO exposure. SOD-Mn mRNA levels increased with treatment with purple carrot extract as well. In conclusion, our results demonstrated that purple carrot extract was able to protect oral lesions induced by 4NQO in Wistar rats as a result of antioxidant activity, anti-inflammatory potential and antiproliferative and antimutagenic actions. |
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Protective effects of purple carrot extract (Daucus carota) against rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide4-Nitroquinoline 1-oxideChemopreventive studiesOral cancerPurple carrotRatThe aim of this study was to evaluate the chemopreventive potential of purple carrot extract following rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide (4NQO). For this purpose, histopathological analysis, proliferative status, antioxidant activity and inflammatory status were investigated in this setting. A total of 20 male rats were distributed into four groups as follows (n = 5 per group): Group 1—free access to water and commercial diet for 12 weeks; Group 2—received 4NQO at 50 ppm dose in drinking water daily and commercial diet for 12 weeks; Group 3—free access to water and received diet supplemented with purple carrot extract (0.1 g/kg) for 12 weeks; and Group 4—received 4NQO at 50 ppm dose in drinking water daily and diet supplemented with purple carrot extract (0.1 g/kg) for 12 weeks. Histopathological analysis revealed that animals treated with purple carrot extract reduced the oral lesions such as dysplasia and squamous cell carcinoma. Animals with oral pre-neoplastic lesions and treated with purple carrot extract decreased ki-67 and 8-OHdG immunoexpression. Moreover, pNFκBp50 and MyD88 protein expressions were decreased after purple carrot treatment associated or not with 4NQO exposure. SOD-Mn mRNA levels increased with treatment with purple carrot extract as well. In conclusion, our results demonstrated that purple carrot extract was able to protect oral lesions induced by 4NQO in Wistar rats as a result of antioxidant activity, anti-inflammatory potential and antiproliferative and antimutagenic actions.Department of Biosciences Federal University of São Paulo (UNIFESP), Rua Silva Jardim, 136, Vila MathiasSao Paulo State University (UNESP), Campus Litoral PaulistaSao Paulo State University (UNESP), Campus Litoral PaulistaUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Soares, Glaucia Resendede Moura, Carolina Foot GomesSilva, Marcelo Jose Dias [UNESP]Vilegas, Wagner [UNESP]Santamarina, Aline BovetoPisani, Luciana PellegriniEstadella, DeboraRibeiro, Daniel Araki2018-12-11T17:18:38Z2018-12-11T17:18:38Z2018-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1007/s12032-018-1114-7Medical Oncology, v. 35, n. 4, 2018.1559-131X1357-0560http://hdl.handle.net/11449/17603410.1007/s12032-018-1114-72-s2.0-850442063502-s2.0-85044206350.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMedical Oncology0,757info:eu-repo/semantics/openAccess2023-11-27T06:12:16Zoai:repositorio.unesp.br:11449/176034Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:50:14.261662Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Protective effects of purple carrot extract (Daucus carota) against rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide |
title |
Protective effects of purple carrot extract (Daucus carota) against rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide |
spellingShingle |
Protective effects of purple carrot extract (Daucus carota) against rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide Soares, Glaucia Resende 4-Nitroquinoline 1-oxide Chemopreventive studies Oral cancer Purple carrot Rat |
title_short |
Protective effects of purple carrot extract (Daucus carota) against rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide |
title_full |
Protective effects of purple carrot extract (Daucus carota) against rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide |
title_fullStr |
Protective effects of purple carrot extract (Daucus carota) against rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide |
title_full_unstemmed |
Protective effects of purple carrot extract (Daucus carota) against rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide |
title_sort |
Protective effects of purple carrot extract (Daucus carota) against rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide |
author |
Soares, Glaucia Resende |
author_facet |
Soares, Glaucia Resende de Moura, Carolina Foot Gomes Silva, Marcelo Jose Dias [UNESP] Vilegas, Wagner [UNESP] Santamarina, Aline Boveto Pisani, Luciana Pellegrini Estadella, Debora Ribeiro, Daniel Araki |
author_role |
author |
author2 |
de Moura, Carolina Foot Gomes Silva, Marcelo Jose Dias [UNESP] Vilegas, Wagner [UNESP] Santamarina, Aline Boveto Pisani, Luciana Pellegrini Estadella, Debora Ribeiro, Daniel Araki |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Soares, Glaucia Resende de Moura, Carolina Foot Gomes Silva, Marcelo Jose Dias [UNESP] Vilegas, Wagner [UNESP] Santamarina, Aline Boveto Pisani, Luciana Pellegrini Estadella, Debora Ribeiro, Daniel Araki |
dc.subject.por.fl_str_mv |
4-Nitroquinoline 1-oxide Chemopreventive studies Oral cancer Purple carrot Rat |
topic |
4-Nitroquinoline 1-oxide Chemopreventive studies Oral cancer Purple carrot Rat |
description |
The aim of this study was to evaluate the chemopreventive potential of purple carrot extract following rat tongue carcinogenesis induced by 4-nitroquinoline 1-oxide (4NQO). For this purpose, histopathological analysis, proliferative status, antioxidant activity and inflammatory status were investigated in this setting. A total of 20 male rats were distributed into four groups as follows (n = 5 per group): Group 1—free access to water and commercial diet for 12 weeks; Group 2—received 4NQO at 50 ppm dose in drinking water daily and commercial diet for 12 weeks; Group 3—free access to water and received diet supplemented with purple carrot extract (0.1 g/kg) for 12 weeks; and Group 4—received 4NQO at 50 ppm dose in drinking water daily and diet supplemented with purple carrot extract (0.1 g/kg) for 12 weeks. Histopathological analysis revealed that animals treated with purple carrot extract reduced the oral lesions such as dysplasia and squamous cell carcinoma. Animals with oral pre-neoplastic lesions and treated with purple carrot extract decreased ki-67 and 8-OHdG immunoexpression. Moreover, pNFκBp50 and MyD88 protein expressions were decreased after purple carrot treatment associated or not with 4NQO exposure. SOD-Mn mRNA levels increased with treatment with purple carrot extract as well. In conclusion, our results demonstrated that purple carrot extract was able to protect oral lesions induced by 4NQO in Wistar rats as a result of antioxidant activity, anti-inflammatory potential and antiproliferative and antimutagenic actions. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:18:38Z 2018-12-11T17:18:38Z 2018-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s12032-018-1114-7 Medical Oncology, v. 35, n. 4, 2018. 1559-131X 1357-0560 http://hdl.handle.net/11449/176034 10.1007/s12032-018-1114-7 2-s2.0-85044206350 2-s2.0-85044206350.pdf |
url |
http://dx.doi.org/10.1007/s12032-018-1114-7 http://hdl.handle.net/11449/176034 |
identifier_str_mv |
Medical Oncology, v. 35, n. 4, 2018. 1559-131X 1357-0560 10.1007/s12032-018-1114-7 2-s2.0-85044206350 2-s2.0-85044206350.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Medical Oncology 0,757 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128988452749312 |