A Bayesian approach to estimate the accuracy of in-house ELISA assay to measure rabies antibodies from compulsory vaccinated dogs and cattle
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-91992009000100012 http://hdl.handle.net/11449/71010 |
Resumo: | Rabies is a vaccine-preventable disease that causes acute encephalitis in mammals, and it is still a significant public health problem in numerous countries. Infected dogs represent the main vectors involved in human rabies. Additionally, cattle rearing close to geographic areas where vampire bats are found presents an important connection with rural epidemiology. We applied two in-house enzyme-linked immunosorbent assay (ELISA) methodologies, considered alternatives to measure antibodies from vaccinated dogs and cattle, without employing the gold standard approach. The ELISA assays were performed on individual serum samples taken from domestic adult dogs and cows compulsory vaccinated against rabies (147 urban dogs and 64 cows; n = 211). The sandwich and liquid-phase competitive ELISA (scELISA and lpcELISA), considered in-house assays, were performed according to previous works. The only statistical methodology that allows this study is the Bayesian approach, developed to replace the conventional Hui-Walter paradigm. For conditional independent Bayesian model (one population, two tests and no gold standard) the prior information for sensitivity and specificity of each test, mode, prevalence and transformed (α, β) were submitted to Bayesian inference. The in-house lpcELISA revealed 16 - out of 261 serum samples - negative results, whereas in scELISA all results were positive. The Bayesian approach showed that prior information was specified for all parameters; posterior medians were Se scELISA 89%, Sp scELISA 88%, Sp lpcELISA 95% Se lpcELISA 98%, and prevalence (pi) of 99%, without the use of gold standard analysis to measure specific anti-rabies antibodies. |
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A Bayesian approach to estimate the accuracy of in-house ELISA assay to measure rabies antibodies from compulsory vaccinated dogs and cattleBayesian analysisELISA assayRabies antibodyrabies immunoglobulinrabies vaccineaccuracyBayes theoremblood samplingcattledogenzyme immunoassayenzyme linked immunosorbent assaygold standardliquidnonhumanprevalencerabiessensitivity and specificityvaccinationBosCanis familiarisDesmodus rotundusMammaliaRabies is a vaccine-preventable disease that causes acute encephalitis in mammals, and it is still a significant public health problem in numerous countries. Infected dogs represent the main vectors involved in human rabies. Additionally, cattle rearing close to geographic areas where vampire bats are found presents an important connection with rural epidemiology. We applied two in-house enzyme-linked immunosorbent assay (ELISA) methodologies, considered alternatives to measure antibodies from vaccinated dogs and cattle, without employing the gold standard approach. The ELISA assays were performed on individual serum samples taken from domestic adult dogs and cows compulsory vaccinated against rabies (147 urban dogs and 64 cows; n = 211). The sandwich and liquid-phase competitive ELISA (scELISA and lpcELISA), considered in-house assays, were performed according to previous works. The only statistical methodology that allows this study is the Bayesian approach, developed to replace the conventional Hui-Walter paradigm. For conditional independent Bayesian model (one population, two tests and no gold standard) the prior information for sensitivity and specificity of each test, mode, prevalence and transformed (α, β) were submitted to Bayesian inference. The in-house lpcELISA revealed 16 - out of 261 serum samples - negative results, whereas in scELISA all results were positive. The Bayesian approach showed that prior information was specified for all parameters; posterior medians were Se scELISA 89%, Sp scELISA 88%, Sp lpcELISA 95% Se lpcELISA 98%, and prevalence (pi) of 99%, without the use of gold standard analysis to measure specific anti-rabies antibodies.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo Agency of Agribusiness Technology APTA, Araçatuba, São Paulo StateDepartment of Animal Health and Production School of Dentistry São Paulo State University, UNESP, Araçatuba, São Paulo StateLaboratory of Veterinary Pathology School of Dentistry São Paulo State University, UNESP, Araçatuba, São Paulo StateDepartamento de Apoio, Produção e Saúde Animal FOA UNESP, Araçatuba, SP, 16.050-680Department of Animal Health and Production School of Dentistry São Paulo State University, UNESP, Araçatuba, São Paulo StateLaboratory of Veterinary Pathology School of Dentistry São Paulo State University, UNESP, Araçatuba, São Paulo StateDepartamento de Apoio, Produção e Saúde Animal FOA UNESP, Araçatuba, SP, 16.050-680Agência Paulista de Tecnologia dos Agronegócios (APTA)Universidade Estadual Paulista (Unesp)Nogueira, A. H CSilva, C. [UNESP]Gomes, D. E. [UNESP]Rosa, A. C G [UNESP]Luvizotto, Maria Cecília Rui [UNESP]Cardoso, Tereza Cristina [UNESP]2014-05-27T11:23:54Z2014-05-27T11:23:54Z2009-05-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article136-145application/pdfhttp://dx.doi.org/10.1590/S1678-91992009000100012Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 15, n. 1, p. 136-145, 2009.1678-9199http://hdl.handle.net/11449/7101010.1590/S1678-91992009000100012S1678-91992009000100012WOS:0002643665000122-s2.0-657491016742-s2.0-65749101674.pdf4584674909952477Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseases1.7820,573info:eu-repo/semantics/openAccess2024-09-19T18:57:28Zoai:repositorio.unesp.br:11449/71010Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T18:57:28Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A Bayesian approach to estimate the accuracy of in-house ELISA assay to measure rabies antibodies from compulsory vaccinated dogs and cattle |
title |
A Bayesian approach to estimate the accuracy of in-house ELISA assay to measure rabies antibodies from compulsory vaccinated dogs and cattle |
spellingShingle |
A Bayesian approach to estimate the accuracy of in-house ELISA assay to measure rabies antibodies from compulsory vaccinated dogs and cattle Nogueira, A. H C Bayesian analysis ELISA assay Rabies antibody rabies immunoglobulin rabies vaccine accuracy Bayes theorem blood sampling cattle dog enzyme immunoassay enzyme linked immunosorbent assay gold standard liquid nonhuman prevalence rabies sensitivity and specificity vaccination Bos Canis familiaris Desmodus rotundus Mammalia |
title_short |
A Bayesian approach to estimate the accuracy of in-house ELISA assay to measure rabies antibodies from compulsory vaccinated dogs and cattle |
title_full |
A Bayesian approach to estimate the accuracy of in-house ELISA assay to measure rabies antibodies from compulsory vaccinated dogs and cattle |
title_fullStr |
A Bayesian approach to estimate the accuracy of in-house ELISA assay to measure rabies antibodies from compulsory vaccinated dogs and cattle |
title_full_unstemmed |
A Bayesian approach to estimate the accuracy of in-house ELISA assay to measure rabies antibodies from compulsory vaccinated dogs and cattle |
title_sort |
A Bayesian approach to estimate the accuracy of in-house ELISA assay to measure rabies antibodies from compulsory vaccinated dogs and cattle |
author |
Nogueira, A. H C |
author_facet |
Nogueira, A. H C Silva, C. [UNESP] Gomes, D. E. [UNESP] Rosa, A. C G [UNESP] Luvizotto, Maria Cecília Rui [UNESP] Cardoso, Tereza Cristina [UNESP] |
author_role |
author |
author2 |
Silva, C. [UNESP] Gomes, D. E. [UNESP] Rosa, A. C G [UNESP] Luvizotto, Maria Cecília Rui [UNESP] Cardoso, Tereza Cristina [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Agência Paulista de Tecnologia dos Agronegócios (APTA) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Nogueira, A. H C Silva, C. [UNESP] Gomes, D. E. [UNESP] Rosa, A. C G [UNESP] Luvizotto, Maria Cecília Rui [UNESP] Cardoso, Tereza Cristina [UNESP] |
dc.subject.por.fl_str_mv |
Bayesian analysis ELISA assay Rabies antibody rabies immunoglobulin rabies vaccine accuracy Bayes theorem blood sampling cattle dog enzyme immunoassay enzyme linked immunosorbent assay gold standard liquid nonhuman prevalence rabies sensitivity and specificity vaccination Bos Canis familiaris Desmodus rotundus Mammalia |
topic |
Bayesian analysis ELISA assay Rabies antibody rabies immunoglobulin rabies vaccine accuracy Bayes theorem blood sampling cattle dog enzyme immunoassay enzyme linked immunosorbent assay gold standard liquid nonhuman prevalence rabies sensitivity and specificity vaccination Bos Canis familiaris Desmodus rotundus Mammalia |
description |
Rabies is a vaccine-preventable disease that causes acute encephalitis in mammals, and it is still a significant public health problem in numerous countries. Infected dogs represent the main vectors involved in human rabies. Additionally, cattle rearing close to geographic areas where vampire bats are found presents an important connection with rural epidemiology. We applied two in-house enzyme-linked immunosorbent assay (ELISA) methodologies, considered alternatives to measure antibodies from vaccinated dogs and cattle, without employing the gold standard approach. The ELISA assays were performed on individual serum samples taken from domestic adult dogs and cows compulsory vaccinated against rabies (147 urban dogs and 64 cows; n = 211). The sandwich and liquid-phase competitive ELISA (scELISA and lpcELISA), considered in-house assays, were performed according to previous works. The only statistical methodology that allows this study is the Bayesian approach, developed to replace the conventional Hui-Walter paradigm. For conditional independent Bayesian model (one population, two tests and no gold standard) the prior information for sensitivity and specificity of each test, mode, prevalence and transformed (α, β) were submitted to Bayesian inference. The in-house lpcELISA revealed 16 - out of 261 serum samples - negative results, whereas in scELISA all results were positive. The Bayesian approach showed that prior information was specified for all parameters; posterior medians were Se scELISA 89%, Sp scELISA 88%, Sp lpcELISA 95% Se lpcELISA 98%, and prevalence (pi) of 99%, without the use of gold standard analysis to measure specific anti-rabies antibodies. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-05-28 2014-05-27T11:23:54Z 2014-05-27T11:23:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-91992009000100012 Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 15, n. 1, p. 136-145, 2009. 1678-9199 http://hdl.handle.net/11449/71010 10.1590/S1678-91992009000100012 S1678-91992009000100012 WOS:000264366500012 2-s2.0-65749101674 2-s2.0-65749101674.pdf 4584674909952477 |
url |
http://dx.doi.org/10.1590/S1678-91992009000100012 http://hdl.handle.net/11449/71010 |
identifier_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 15, n. 1, p. 136-145, 2009. 1678-9199 10.1590/S1678-91992009000100012 S1678-91992009000100012 WOS:000264366500012 2-s2.0-65749101674 2-s2.0-65749101674.pdf 4584674909952477 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases 1.782 0,573 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
136-145 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546485492482048 |