A Bayesian approach to estimate the accuracy of in-house ELISA assay to measure rabies antibodies from compulsory vaccinated dogs and cattle

Detalhes bibliográficos
Autor(a) principal: Nogueira, A. H C
Data de Publicação: 2009
Outros Autores: Silva, C. [UNESP], Gomes, D. E. [UNESP], Rosa, A. C G [UNESP], Luvizotto, Maria Cecília Rui [UNESP], Cardoso, Tereza Cristina [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1678-91992009000100012
http://hdl.handle.net/11449/71010
Resumo: Rabies is a vaccine-preventable disease that causes acute encephalitis in mammals, and it is still a significant public health problem in numerous countries. Infected dogs represent the main vectors involved in human rabies. Additionally, cattle rearing close to geographic areas where vampire bats are found presents an important connection with rural epidemiology. We applied two in-house enzyme-linked immunosorbent assay (ELISA) methodologies, considered alternatives to measure antibodies from vaccinated dogs and cattle, without employing the gold standard approach. The ELISA assays were performed on individual serum samples taken from domestic adult dogs and cows compulsory vaccinated against rabies (147 urban dogs and 64 cows; n = 211). The sandwich and liquid-phase competitive ELISA (scELISA and lpcELISA), considered in-house assays, were performed according to previous works. The only statistical methodology that allows this study is the Bayesian approach, developed to replace the conventional Hui-Walter paradigm. For conditional independent Bayesian model (one population, two tests and no gold standard) the prior information for sensitivity and specificity of each test, mode, prevalence and transformed (α, β) were submitted to Bayesian inference. The in-house lpcELISA revealed 16 - out of 261 serum samples - negative results, whereas in scELISA all results were positive. The Bayesian approach showed that prior information was specified for all parameters; posterior medians were Se scELISA 89%, Sp scELISA 88%, Sp lpcELISA 95% Se lpcELISA 98%, and prevalence (pi) of 99%, without the use of gold standard analysis to measure specific anti-rabies antibodies.
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spelling A Bayesian approach to estimate the accuracy of in-house ELISA assay to measure rabies antibodies from compulsory vaccinated dogs and cattleBayesian analysisELISA assayRabies antibodyrabies immunoglobulinrabies vaccineaccuracyBayes theoremblood samplingcattledogenzyme immunoassayenzyme linked immunosorbent assaygold standardliquidnonhumanprevalencerabiessensitivity and specificityvaccinationBosCanis familiarisDesmodus rotundusMammaliaRabies is a vaccine-preventable disease that causes acute encephalitis in mammals, and it is still a significant public health problem in numerous countries. Infected dogs represent the main vectors involved in human rabies. Additionally, cattle rearing close to geographic areas where vampire bats are found presents an important connection with rural epidemiology. We applied two in-house enzyme-linked immunosorbent assay (ELISA) methodologies, considered alternatives to measure antibodies from vaccinated dogs and cattle, without employing the gold standard approach. The ELISA assays were performed on individual serum samples taken from domestic adult dogs and cows compulsory vaccinated against rabies (147 urban dogs and 64 cows; n = 211). The sandwich and liquid-phase competitive ELISA (scELISA and lpcELISA), considered in-house assays, were performed according to previous works. The only statistical methodology that allows this study is the Bayesian approach, developed to replace the conventional Hui-Walter paradigm. For conditional independent Bayesian model (one population, two tests and no gold standard) the prior information for sensitivity and specificity of each test, mode, prevalence and transformed (α, β) were submitted to Bayesian inference. The in-house lpcELISA revealed 16 - out of 261 serum samples - negative results, whereas in scELISA all results were positive. The Bayesian approach showed that prior information was specified for all parameters; posterior medians were Se scELISA 89%, Sp scELISA 88%, Sp lpcELISA 95% Se lpcELISA 98%, and prevalence (pi) of 99%, without the use of gold standard analysis to measure specific anti-rabies antibodies.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo Agency of Agribusiness Technology APTA, Araçatuba, São Paulo StateDepartment of Animal Health and Production School of Dentistry São Paulo State University, UNESP, Araçatuba, São Paulo StateLaboratory of Veterinary Pathology School of Dentistry São Paulo State University, UNESP, Araçatuba, São Paulo StateDepartamento de Apoio, Produção e Saúde Animal FOA UNESP, Araçatuba, SP, 16.050-680Department of Animal Health and Production School of Dentistry São Paulo State University, UNESP, Araçatuba, São Paulo StateLaboratory of Veterinary Pathology School of Dentistry São Paulo State University, UNESP, Araçatuba, São Paulo StateDepartamento de Apoio, Produção e Saúde Animal FOA UNESP, Araçatuba, SP, 16.050-680Agência Paulista de Tecnologia dos Agronegócios (APTA)Universidade Estadual Paulista (Unesp)Nogueira, A. H CSilva, C. [UNESP]Gomes, D. E. [UNESP]Rosa, A. C G [UNESP]Luvizotto, Maria Cecília Rui [UNESP]Cardoso, Tereza Cristina [UNESP]2014-05-27T11:23:54Z2014-05-27T11:23:54Z2009-05-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article136-145application/pdfhttp://dx.doi.org/10.1590/S1678-91992009000100012Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 15, n. 1, p. 136-145, 2009.1678-9199http://hdl.handle.net/11449/7101010.1590/S1678-91992009000100012S1678-91992009000100012WOS:0002643665000122-s2.0-657491016742-s2.0-65749101674.pdf4584674909952477Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseases1.7820,573info:eu-repo/semantics/openAccess2024-09-19T18:57:28Zoai:repositorio.unesp.br:11449/71010Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-19T18:57:28Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv A Bayesian approach to estimate the accuracy of in-house ELISA assay to measure rabies antibodies from compulsory vaccinated dogs and cattle
title A Bayesian approach to estimate the accuracy of in-house ELISA assay to measure rabies antibodies from compulsory vaccinated dogs and cattle
spellingShingle A Bayesian approach to estimate the accuracy of in-house ELISA assay to measure rabies antibodies from compulsory vaccinated dogs and cattle
Nogueira, A. H C
Bayesian analysis
ELISA assay
Rabies antibody
rabies immunoglobulin
rabies vaccine
accuracy
Bayes theorem
blood sampling
cattle
dog
enzyme immunoassay
enzyme linked immunosorbent assay
gold standard
liquid
nonhuman
prevalence
rabies
sensitivity and specificity
vaccination
Bos
Canis familiaris
Desmodus rotundus
Mammalia
title_short A Bayesian approach to estimate the accuracy of in-house ELISA assay to measure rabies antibodies from compulsory vaccinated dogs and cattle
title_full A Bayesian approach to estimate the accuracy of in-house ELISA assay to measure rabies antibodies from compulsory vaccinated dogs and cattle
title_fullStr A Bayesian approach to estimate the accuracy of in-house ELISA assay to measure rabies antibodies from compulsory vaccinated dogs and cattle
title_full_unstemmed A Bayesian approach to estimate the accuracy of in-house ELISA assay to measure rabies antibodies from compulsory vaccinated dogs and cattle
title_sort A Bayesian approach to estimate the accuracy of in-house ELISA assay to measure rabies antibodies from compulsory vaccinated dogs and cattle
author Nogueira, A. H C
author_facet Nogueira, A. H C
Silva, C. [UNESP]
Gomes, D. E. [UNESP]
Rosa, A. C G [UNESP]
Luvizotto, Maria Cecília Rui [UNESP]
Cardoso, Tereza Cristina [UNESP]
author_role author
author2 Silva, C. [UNESP]
Gomes, D. E. [UNESP]
Rosa, A. C G [UNESP]
Luvizotto, Maria Cecília Rui [UNESP]
Cardoso, Tereza Cristina [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Agência Paulista de Tecnologia dos Agronegócios (APTA)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Nogueira, A. H C
Silva, C. [UNESP]
Gomes, D. E. [UNESP]
Rosa, A. C G [UNESP]
Luvizotto, Maria Cecília Rui [UNESP]
Cardoso, Tereza Cristina [UNESP]
dc.subject.por.fl_str_mv Bayesian analysis
ELISA assay
Rabies antibody
rabies immunoglobulin
rabies vaccine
accuracy
Bayes theorem
blood sampling
cattle
dog
enzyme immunoassay
enzyme linked immunosorbent assay
gold standard
liquid
nonhuman
prevalence
rabies
sensitivity and specificity
vaccination
Bos
Canis familiaris
Desmodus rotundus
Mammalia
topic Bayesian analysis
ELISA assay
Rabies antibody
rabies immunoglobulin
rabies vaccine
accuracy
Bayes theorem
blood sampling
cattle
dog
enzyme immunoassay
enzyme linked immunosorbent assay
gold standard
liquid
nonhuman
prevalence
rabies
sensitivity and specificity
vaccination
Bos
Canis familiaris
Desmodus rotundus
Mammalia
description Rabies is a vaccine-preventable disease that causes acute encephalitis in mammals, and it is still a significant public health problem in numerous countries. Infected dogs represent the main vectors involved in human rabies. Additionally, cattle rearing close to geographic areas where vampire bats are found presents an important connection with rural epidemiology. We applied two in-house enzyme-linked immunosorbent assay (ELISA) methodologies, considered alternatives to measure antibodies from vaccinated dogs and cattle, without employing the gold standard approach. The ELISA assays were performed on individual serum samples taken from domestic adult dogs and cows compulsory vaccinated against rabies (147 urban dogs and 64 cows; n = 211). The sandwich and liquid-phase competitive ELISA (scELISA and lpcELISA), considered in-house assays, were performed according to previous works. The only statistical methodology that allows this study is the Bayesian approach, developed to replace the conventional Hui-Walter paradigm. For conditional independent Bayesian model (one population, two tests and no gold standard) the prior information for sensitivity and specificity of each test, mode, prevalence and transformed (α, β) were submitted to Bayesian inference. The in-house lpcELISA revealed 16 - out of 261 serum samples - negative results, whereas in scELISA all results were positive. The Bayesian approach showed that prior information was specified for all parameters; posterior medians were Se scELISA 89%, Sp scELISA 88%, Sp lpcELISA 95% Se lpcELISA 98%, and prevalence (pi) of 99%, without the use of gold standard analysis to measure specific anti-rabies antibodies.
publishDate 2009
dc.date.none.fl_str_mv 2009-05-28
2014-05-27T11:23:54Z
2014-05-27T11:23:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1678-91992009000100012
Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 15, n. 1, p. 136-145, 2009.
1678-9199
http://hdl.handle.net/11449/71010
10.1590/S1678-91992009000100012
S1678-91992009000100012
WOS:000264366500012
2-s2.0-65749101674
2-s2.0-65749101674.pdf
4584674909952477
url http://dx.doi.org/10.1590/S1678-91992009000100012
http://hdl.handle.net/11449/71010
identifier_str_mv Journal of Venomous Animals and Toxins Including Tropical Diseases, v. 15, n. 1, p. 136-145, 2009.
1678-9199
10.1590/S1678-91992009000100012
S1678-91992009000100012
WOS:000264366500012
2-s2.0-65749101674
2-s2.0-65749101674.pdf
4584674909952477
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Venomous Animals and Toxins Including Tropical Diseases
1.782
0,573
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 136-145
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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