Influence of nitric oxide and phosphodiesterases during in vitro maturation of bovine oocytes on meiotic resumption and embryo production

Detalhes bibliográficos
Autor(a) principal: Botigelli, Ramon Cesar
Data de Publicação: 2017
Outros Autores: Schwarz, Katia Lancellotti, Zaffalon, Fabiane Gilli, Del Collado, Maite, Castro, Fernanda Cavallari, Fernandes, Hugo, Leal, Claudia Lima Verde
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1017/S096719941700017X
http://hdl.handle.net/11449/231403
Resumo: This study aimed to examine the effects of nitric oxide (NO) and different phosphodiesterase (PDE) families on meiosis resumption, nucleotides levels and embryo production. Experiment I, COCs were matured in vitro with the NO donor S-nitroso-N-acetylpenicillamine (SNAP) associated or not with the soluble guanylate cyclase (sGC) inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), meiotic resumption and nucleotides levels were assessed. SNAP delayed germinal vesicle breakdown (GVBD) (53.4 ± 1.2 versus 78.4 ± 2.4% for controls, P < 0.05) and ODQ reversed its effect (73.4 ± 6.3%, P > 0.05). Cyclic GMP levels were higher in SNAP (3.94 ± 0.18, P < 0.05) and ODQ abolished the effect (2.48 ± 0.13 pmol/COC, P < 0.05), while cAMP levels were decreased in both treatments. Experiment II, COCs were cultured with SNAP alone or with PDEs inhibitors. SNAP alone or with PDEs inhibitors delayed GVBD (24.7 ± 2.8 to 56.9 ± 8.7%, P < 0.05) compared with the control (77.1 ± 1.8%), and SNAP and SNAP + cilostamide had lowest rates (34.9 ± 9.2% and 24.7 ± 2.8%). Experiment III, COCs were cultured (24-28 h) with SNAP and SNAP + cilostamide to assess metaphase II (MII) rates and embryo production. SNAP + cilostamide (50.0 ± 2.0%, P < 0.05) had lower MII rates at 24 h in vitro maturation (IVM), but at 28 h all groups were similar (66.6 to 71.4%, P > 0.05). Embryo development did not differ from the control for SNAP and cilostamide groups (38.7 ± 5.8, 37.9 ± 6.2 and 40.5 ± 5.8%, P > 0.05), but SNAP + cilostamide decreased embryo production (25.7 ± 6.9%, P < 0.05). In conclusion, SNAP was confirmed to delay meiosis resumption by the NO/sGC/cGMP pathway, by increasing cGMP, but not cAMP. Inhibiting different PDEs to further increase nucleotides in association with SNAP did not show any additive effects on meiosis resumption, indicating that other pathways are involved. Moreover, SNAP + cilostamide affected the meiosis progression and decreased embryo development.
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spelling Influence of nitric oxide and phosphodiesterases during in vitro maturation of bovine oocytes on meiotic resumption and embryo productioncAMPCattlecGMPCyclic nucleotidesSoluble guanylate cyclaseThis study aimed to examine the effects of nitric oxide (NO) and different phosphodiesterase (PDE) families on meiosis resumption, nucleotides levels and embryo production. Experiment I, COCs were matured in vitro with the NO donor S-nitroso-N-acetylpenicillamine (SNAP) associated or not with the soluble guanylate cyclase (sGC) inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), meiotic resumption and nucleotides levels were assessed. SNAP delayed germinal vesicle breakdown (GVBD) (53.4 ± 1.2 versus 78.4 ± 2.4% for controls, P < 0.05) and ODQ reversed its effect (73.4 ± 6.3%, P > 0.05). Cyclic GMP levels were higher in SNAP (3.94 ± 0.18, P < 0.05) and ODQ abolished the effect (2.48 ± 0.13 pmol/COC, P < 0.05), while cAMP levels were decreased in both treatments. Experiment II, COCs were cultured with SNAP alone or with PDEs inhibitors. SNAP alone or with PDEs inhibitors delayed GVBD (24.7 ± 2.8 to 56.9 ± 8.7%, P < 0.05) compared with the control (77.1 ± 1.8%), and SNAP and SNAP + cilostamide had lowest rates (34.9 ± 9.2% and 24.7 ± 2.8%). Experiment III, COCs were cultured (24-28 h) with SNAP and SNAP + cilostamide to assess metaphase II (MII) rates and embryo production. SNAP + cilostamide (50.0 ± 2.0%, P < 0.05) had lower MII rates at 24 h in vitro maturation (IVM), but at 28 h all groups were similar (66.6 to 71.4%, P > 0.05). Embryo development did not differ from the control for SNAP and cilostamide groups (38.7 ± 5.8, 37.9 ± 6.2 and 40.5 ± 5.8%, P > 0.05), but SNAP + cilostamide decreased embryo production (25.7 ± 6.9%, P < 0.05). In conclusion, SNAP was confirmed to delay meiosis resumption by the NO/sGC/cGMP pathway, by increasing cGMP, but not cAMP. Inhibiting different PDEs to further increase nucleotides in association with SNAP did not show any additive effects on meiosis resumption, indicating that other pathways are involved. Moreover, SNAP + cilostamide affected the meiosis progression and decreased embryo development.Departamento de Medicina Veterinária Faculdade de Zootecnia e Engenharia de Alimentos Universidade de São Paulo, Av. Duque de Caxias Norte, 225Departamento de Farmacologia Instituto de Biociências de Botucatu Universidade Do Estado de São Paulo, Distrito de Rubião JuniorUniversidade de São Paulo (USP)Universidade Do Estado de São PauloBotigelli, Ramon CesarSchwarz, Katia LancellottiZaffalon, Fabiane GilliDel Collado, MaiteCastro, Fernanda CavallariFernandes, HugoLeal, Claudia Lima Verde2022-04-29T08:45:17Z2022-04-29T08:45:17Z2017-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article321-330http://dx.doi.org/10.1017/S096719941700017XZygote, v. 25, n. 3, p. 321-330, 2017.1469-87300967-1994http://hdl.handle.net/11449/23140310.1017/S096719941700017X2-s2.0-85021254981Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengZygoteinfo:eu-repo/semantics/openAccess2022-04-29T08:45:17Zoai:repositorio.unesp.br:11449/231403Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:36:18.508631Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Influence of nitric oxide and phosphodiesterases during in vitro maturation of bovine oocytes on meiotic resumption and embryo production
title Influence of nitric oxide and phosphodiesterases during in vitro maturation of bovine oocytes on meiotic resumption and embryo production
spellingShingle Influence of nitric oxide and phosphodiesterases during in vitro maturation of bovine oocytes on meiotic resumption and embryo production
Botigelli, Ramon Cesar
cAMP
Cattle
cGMP
Cyclic nucleotides
Soluble guanylate cyclase
title_short Influence of nitric oxide and phosphodiesterases during in vitro maturation of bovine oocytes on meiotic resumption and embryo production
title_full Influence of nitric oxide and phosphodiesterases during in vitro maturation of bovine oocytes on meiotic resumption and embryo production
title_fullStr Influence of nitric oxide and phosphodiesterases during in vitro maturation of bovine oocytes on meiotic resumption and embryo production
title_full_unstemmed Influence of nitric oxide and phosphodiesterases during in vitro maturation of bovine oocytes on meiotic resumption and embryo production
title_sort Influence of nitric oxide and phosphodiesterases during in vitro maturation of bovine oocytes on meiotic resumption and embryo production
author Botigelli, Ramon Cesar
author_facet Botigelli, Ramon Cesar
Schwarz, Katia Lancellotti
Zaffalon, Fabiane Gilli
Del Collado, Maite
Castro, Fernanda Cavallari
Fernandes, Hugo
Leal, Claudia Lima Verde
author_role author
author2 Schwarz, Katia Lancellotti
Zaffalon, Fabiane Gilli
Del Collado, Maite
Castro, Fernanda Cavallari
Fernandes, Hugo
Leal, Claudia Lima Verde
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
Universidade Do Estado de São Paulo
dc.contributor.author.fl_str_mv Botigelli, Ramon Cesar
Schwarz, Katia Lancellotti
Zaffalon, Fabiane Gilli
Del Collado, Maite
Castro, Fernanda Cavallari
Fernandes, Hugo
Leal, Claudia Lima Verde
dc.subject.por.fl_str_mv cAMP
Cattle
cGMP
Cyclic nucleotides
Soluble guanylate cyclase
topic cAMP
Cattle
cGMP
Cyclic nucleotides
Soluble guanylate cyclase
description This study aimed to examine the effects of nitric oxide (NO) and different phosphodiesterase (PDE) families on meiosis resumption, nucleotides levels and embryo production. Experiment I, COCs were matured in vitro with the NO donor S-nitroso-N-acetylpenicillamine (SNAP) associated or not with the soluble guanylate cyclase (sGC) inhibitor 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), meiotic resumption and nucleotides levels were assessed. SNAP delayed germinal vesicle breakdown (GVBD) (53.4 ± 1.2 versus 78.4 ± 2.4% for controls, P < 0.05) and ODQ reversed its effect (73.4 ± 6.3%, P > 0.05). Cyclic GMP levels were higher in SNAP (3.94 ± 0.18, P < 0.05) and ODQ abolished the effect (2.48 ± 0.13 pmol/COC, P < 0.05), while cAMP levels were decreased in both treatments. Experiment II, COCs were cultured with SNAP alone or with PDEs inhibitors. SNAP alone or with PDEs inhibitors delayed GVBD (24.7 ± 2.8 to 56.9 ± 8.7%, P < 0.05) compared with the control (77.1 ± 1.8%), and SNAP and SNAP + cilostamide had lowest rates (34.9 ± 9.2% and 24.7 ± 2.8%). Experiment III, COCs were cultured (24-28 h) with SNAP and SNAP + cilostamide to assess metaphase II (MII) rates and embryo production. SNAP + cilostamide (50.0 ± 2.0%, P < 0.05) had lower MII rates at 24 h in vitro maturation (IVM), but at 28 h all groups were similar (66.6 to 71.4%, P > 0.05). Embryo development did not differ from the control for SNAP and cilostamide groups (38.7 ± 5.8, 37.9 ± 6.2 and 40.5 ± 5.8%, P > 0.05), but SNAP + cilostamide decreased embryo production (25.7 ± 6.9%, P < 0.05). In conclusion, SNAP was confirmed to delay meiosis resumption by the NO/sGC/cGMP pathway, by increasing cGMP, but not cAMP. Inhibiting different PDEs to further increase nucleotides in association with SNAP did not show any additive effects on meiosis resumption, indicating that other pathways are involved. Moreover, SNAP + cilostamide affected the meiosis progression and decreased embryo development.
publishDate 2017
dc.date.none.fl_str_mv 2017-06-01
2022-04-29T08:45:17Z
2022-04-29T08:45:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1017/S096719941700017X
Zygote, v. 25, n. 3, p. 321-330, 2017.
1469-8730
0967-1994
http://hdl.handle.net/11449/231403
10.1017/S096719941700017X
2-s2.0-85021254981
url http://dx.doi.org/10.1017/S096719941700017X
http://hdl.handle.net/11449/231403
identifier_str_mv Zygote, v. 25, n. 3, p. 321-330, 2017.
1469-8730
0967-1994
10.1017/S096719941700017X
2-s2.0-85021254981
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Zygote
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 321-330
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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