Evaluation of Caenorhabditis elegans as a host model for Paracoccidioides brasiliensis and Paracoccidioides lutzii

• Autor(a) principal: Scorzoni, Liliana [UNESP]
• Data de Publicação: 2018
• Outros Autores: de Lucas, Maria Pilar, Singulani, Junya de Lacorte [UNESP], de Oliveira, Haroldo Cesar [UNESP], Assato, Patricia Akemi [UNESP], Fusco-Almeida, Ana Marisa [UNESP], Mendes-Giannini, Maria José Soares [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1093/femspd/fty004; http://hdl.handle.net/11449/176083
• topic: Caenorhabditis elegans; Fungal infection model; Immunomodulation; Innate immune response; Paracoccidioides brasiliensis; Paracoccidioides lutzii

Paracoccidioidomycosis is a systemic fungal infection affecting mainly Latin American countries that is caused by Paracoccidioides brasiliensis and Paracoccidioides lutzii. During the study of fungal pathogenesis, in vivo studies are crucial to understand the overall mechanisms involving the infection as well as to search for new therapeutic treatments and diagnosis. Caenorhabditis elegans is described as an infection model for different fungi species and a well-characterized organism to study the innate immune response. This study evaluates C. elegans as an infection model for Paracoccidioides spp. It was observed that both species do not cause infection in C. elegans, as occurs with Candida albicans, and one possible explanation is that the irregular size and shape of Paracoccidioides spp. difficult the ingestion of these fungi by the nematode. Besides this difficulty in the infection, we could observe that the simple exposition of C. elegans to Paracoccidioides species was able to trigger a distinct pattern of expression of antimicrobial peptide genes. The expression of cnc-4, nlpl-27 and nlp-31 was superior after the exposure to P. brasiliensis in comparison to P. lutzii (P < 0.05), and these findings demonstrate important differences regarding innate immune response activation caused by the two species of the Paracoccidioides genus.