Thyroid Hormones Deficiency Impairs Male Germ Cell Development: A Cross Talk Between Hypothalamic-Pituitary-Thyroid, and—Gonadal Axes in Zebrafish
Autor(a) principal: | |
---|---|
Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3389/fcell.2022.865948 http://hdl.handle.net/11449/241092 |
Resumo: | In vertebrates, thyroid hormones are critical players in controlling different physiological processes such as development, growth, metabolism among others. There is evidence in mammals that thyroid hormones are also an important component of the hormonal system that controls reproduction, although studies in fish remain poorly investigated. Here, we tested this hypothesis by investigating the effects of methimazole-induced hypothyroidism on the testicular function in adult zebrafish. Treatment of fish with methimazole, in vivo, significantly altered zebrafish spermatogenesis by inhibiting cell differentiation and meiosis, as well as decreasing the relative number of spermatozoa. The observed impairment of spermatogenesis by methimazole was correlated with significant changes in transcript levels for several genes implicated in the control of reproduction. Using an in vitro approach, we also demonstrated that in addition to affecting the components of the brain-pituitary-peripheral axis, T3 (triiodothyronine) also exerts direct action on the testis. These results reinforce the hypothesis that thyroid hormones are an essential element of multifactorial control of reproduction and testicular function in zebrafish and possibly other vertebrate species. |
id |
UNSP_27e78121b1b3eb4c17c126de41efa8be |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/241092 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Thyroid Hormones Deficiency Impairs Male Germ Cell Development: A Cross Talk Between Hypothalamic-Pituitary-Thyroid, and—Gonadal Axes in Zebrafishgerm cellhypothyroidismmethimazolespermatogenesisthyroid hormoneszebrafishIn vertebrates, thyroid hormones are critical players in controlling different physiological processes such as development, growth, metabolism among others. There is evidence in mammals that thyroid hormones are also an important component of the hormonal system that controls reproduction, although studies in fish remain poorly investigated. Here, we tested this hypothesis by investigating the effects of methimazole-induced hypothyroidism on the testicular function in adult zebrafish. Treatment of fish with methimazole, in vivo, significantly altered zebrafish spermatogenesis by inhibiting cell differentiation and meiosis, as well as decreasing the relative number of spermatozoa. The observed impairment of spermatogenesis by methimazole was correlated with significant changes in transcript levels for several genes implicated in the control of reproduction. Using an in vitro approach, we also demonstrated that in addition to affecting the components of the brain-pituitary-peripheral axis, T3 (triiodothyronine) also exerts direct action on the testis. These results reinforce the hypothesis that thyroid hormones are an essential element of multifactorial control of reproduction and testicular function in zebrafish and possibly other vertebrate species.Canadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of CanadaFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Natural Sciences and Engineering Research Council of CanadaAquaculture Program (CAUNESP) São Paulo State University (UNESP)Reproductive and Molecular Biology Group Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP)Department of Biological Sciences University of CalgaryAquaculture Program (CAUNESP) São Paulo State University (UNESP)Reproductive and Molecular Biology Group Department of Structural and Functional Biology Institute of Biosciences São Paulo State University (UNESP)Universidade Estadual Paulista (UNESP)University of CalgaryRodrigues, Maira S. [UNESP]Tovo-Neto, Aldo [UNESP]Rosa, Ivana F. [UNESP]Doretto, Lucas B. [UNESP]Fallah, Hamideh P.Habibi, Hamid R.Nóbrega, Rafael H. [UNESP]2023-03-01T20:46:42Z2023-03-01T20:46:42Z2022-05-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3389/fcell.2022.865948Frontiers in Cell and Developmental Biology, v. 10.2296-634Xhttp://hdl.handle.net/11449/24109210.3389/fcell.2022.8659482-s2.0-85131196584Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFrontiers in Cell and Developmental Biologyinfo:eu-repo/semantics/openAccess2024-04-09T15:29:58Zoai:repositorio.unesp.br:11449/241092Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-09T15:29:58Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Thyroid Hormones Deficiency Impairs Male Germ Cell Development: A Cross Talk Between Hypothalamic-Pituitary-Thyroid, and—Gonadal Axes in Zebrafish |
title |
Thyroid Hormones Deficiency Impairs Male Germ Cell Development: A Cross Talk Between Hypothalamic-Pituitary-Thyroid, and—Gonadal Axes in Zebrafish |
spellingShingle |
Thyroid Hormones Deficiency Impairs Male Germ Cell Development: A Cross Talk Between Hypothalamic-Pituitary-Thyroid, and—Gonadal Axes in Zebrafish Rodrigues, Maira S. [UNESP] germ cell hypothyroidism methimazole spermatogenesis thyroid hormones zebrafish |
title_short |
Thyroid Hormones Deficiency Impairs Male Germ Cell Development: A Cross Talk Between Hypothalamic-Pituitary-Thyroid, and—Gonadal Axes in Zebrafish |
title_full |
Thyroid Hormones Deficiency Impairs Male Germ Cell Development: A Cross Talk Between Hypothalamic-Pituitary-Thyroid, and—Gonadal Axes in Zebrafish |
title_fullStr |
Thyroid Hormones Deficiency Impairs Male Germ Cell Development: A Cross Talk Between Hypothalamic-Pituitary-Thyroid, and—Gonadal Axes in Zebrafish |
title_full_unstemmed |
Thyroid Hormones Deficiency Impairs Male Germ Cell Development: A Cross Talk Between Hypothalamic-Pituitary-Thyroid, and—Gonadal Axes in Zebrafish |
title_sort |
Thyroid Hormones Deficiency Impairs Male Germ Cell Development: A Cross Talk Between Hypothalamic-Pituitary-Thyroid, and—Gonadal Axes in Zebrafish |
author |
Rodrigues, Maira S. [UNESP] |
author_facet |
Rodrigues, Maira S. [UNESP] Tovo-Neto, Aldo [UNESP] Rosa, Ivana F. [UNESP] Doretto, Lucas B. [UNESP] Fallah, Hamideh P. Habibi, Hamid R. Nóbrega, Rafael H. [UNESP] |
author_role |
author |
author2 |
Tovo-Neto, Aldo [UNESP] Rosa, Ivana F. [UNESP] Doretto, Lucas B. [UNESP] Fallah, Hamideh P. Habibi, Hamid R. Nóbrega, Rafael H. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) University of Calgary |
dc.contributor.author.fl_str_mv |
Rodrigues, Maira S. [UNESP] Tovo-Neto, Aldo [UNESP] Rosa, Ivana F. [UNESP] Doretto, Lucas B. [UNESP] Fallah, Hamideh P. Habibi, Hamid R. Nóbrega, Rafael H. [UNESP] |
dc.subject.por.fl_str_mv |
germ cell hypothyroidism methimazole spermatogenesis thyroid hormones zebrafish |
topic |
germ cell hypothyroidism methimazole spermatogenesis thyroid hormones zebrafish |
description |
In vertebrates, thyroid hormones are critical players in controlling different physiological processes such as development, growth, metabolism among others. There is evidence in mammals that thyroid hormones are also an important component of the hormonal system that controls reproduction, although studies in fish remain poorly investigated. Here, we tested this hypothesis by investigating the effects of methimazole-induced hypothyroidism on the testicular function in adult zebrafish. Treatment of fish with methimazole, in vivo, significantly altered zebrafish spermatogenesis by inhibiting cell differentiation and meiosis, as well as decreasing the relative number of spermatozoa. The observed impairment of spermatogenesis by methimazole was correlated with significant changes in transcript levels for several genes implicated in the control of reproduction. Using an in vitro approach, we also demonstrated that in addition to affecting the components of the brain-pituitary-peripheral axis, T3 (triiodothyronine) also exerts direct action on the testis. These results reinforce the hypothesis that thyroid hormones are an essential element of multifactorial control of reproduction and testicular function in zebrafish and possibly other vertebrate species. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-05-12 2023-03-01T20:46:42Z 2023-03-01T20:46:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3389/fcell.2022.865948 Frontiers in Cell and Developmental Biology, v. 10. 2296-634X http://hdl.handle.net/11449/241092 10.3389/fcell.2022.865948 2-s2.0-85131196584 |
url |
http://dx.doi.org/10.3389/fcell.2022.865948 http://hdl.handle.net/11449/241092 |
identifier_str_mv |
Frontiers in Cell and Developmental Biology, v. 10. 2296-634X 10.3389/fcell.2022.865948 2-s2.0-85131196584 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Frontiers in Cell and Developmental Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965018661322752 |