Applicability of papain solutions in immunohematology

Detalhes bibliográficos
Autor(a) principal: De Santis, Lais Priscila [UNESP]
Data de Publicação: 2019
Outros Autores: Garcia, Patricia Carvalho [UNESP], Dias Paes Secco, Valeria Nogueira [UNESP], Ferreira, Rosana Rossi [UNESP], Deffune, Elenice [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.31744/einstein_journal/2019AO4328
http://hdl.handle.net/11449/186840
Resumo: Objective: To compare the enzyme activity of different presentations of papain solution to validate in-house preparations. Methods: Two papain solutions were prepared, and the third presentation was a commercial solution. Tests were carried out with samples of red cells typed as weak RhD. Results: In-house prepared papain solutions showed similar enzyme reactivity, and statistically no differences compared to the enzyme activity of the commercial solution. Conclusion: Evaluating the cost-benefit ratio, the in-house prepared papain solutions present more economic advantages, and can be incorporated into immunohematological routines as a way to cope with periods of financial crisis and cost-containment policies.
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spelling Applicability of papain solutions in immunohematologyAntigensAllergy and immunologyPapainEnzymesObjective: To compare the enzyme activity of different presentations of papain solution to validate in-house preparations. Methods: Two papain solutions were prepared, and the third presentation was a commercial solution. Tests were carried out with samples of red cells typed as weak RhD. Results: In-house prepared papain solutions showed similar enzyme reactivity, and statistically no differences compared to the enzyme activity of the commercial solution. Conclusion: Evaluating the cost-benefit ratio, the in-house prepared papain solutions present more economic advantages, and can be incorporated into immunohematological routines as a way to cope with periods of financial crisis and cost-containment policies.Univ Estadual Paulista, Botucatu, SP, BrazilUniv Estadual Paulista, Bauru, SP, BrazilUniv Estadual Paulista, Botucatu, SP, BrazilUniv Estadual Paulista, Bauru, SP, BrazilInst Israelita Ensino & Pesquisa Albert EinsteinUniversidade Estadual Paulista (Unesp)De Santis, Lais Priscila [UNESP]Garcia, Patricia Carvalho [UNESP]Dias Paes Secco, Valeria Nogueira [UNESP]Ferreira, Rosana Rossi [UNESP]Deffune, Elenice [UNESP]2019-10-06T07:30:36Z2019-10-06T07:30:36Z2019-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article6application/pdfhttp://dx.doi.org/10.31744/einstein_journal/2019AO4328Einstein-sao Paulo. Sao Paulo Sp: Inst Israelita Ensino & Pesquisa Albert Einstein, v. 17, n. 2, 6 p., 2019.1679-4508http://hdl.handle.net/11449/18684010.31744/einstein_journal/2019AO4328S1679-45082019000200208WOS:000483399500002S1679-45082019000200208.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEinstein-sao Pauloinfo:eu-repo/semantics/openAccess2024-09-03T14:29:56Zoai:repositorio.unesp.br:11449/186840Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T14:29:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Applicability of papain solutions in immunohematology
title Applicability of papain solutions in immunohematology
spellingShingle Applicability of papain solutions in immunohematology
De Santis, Lais Priscila [UNESP]
Antigens
Allergy and immunology
Papain
Enzymes
title_short Applicability of papain solutions in immunohematology
title_full Applicability of papain solutions in immunohematology
title_fullStr Applicability of papain solutions in immunohematology
title_full_unstemmed Applicability of papain solutions in immunohematology
title_sort Applicability of papain solutions in immunohematology
author De Santis, Lais Priscila [UNESP]
author_facet De Santis, Lais Priscila [UNESP]
Garcia, Patricia Carvalho [UNESP]
Dias Paes Secco, Valeria Nogueira [UNESP]
Ferreira, Rosana Rossi [UNESP]
Deffune, Elenice [UNESP]
author_role author
author2 Garcia, Patricia Carvalho [UNESP]
Dias Paes Secco, Valeria Nogueira [UNESP]
Ferreira, Rosana Rossi [UNESP]
Deffune, Elenice [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv De Santis, Lais Priscila [UNESP]
Garcia, Patricia Carvalho [UNESP]
Dias Paes Secco, Valeria Nogueira [UNESP]
Ferreira, Rosana Rossi [UNESP]
Deffune, Elenice [UNESP]
dc.subject.por.fl_str_mv Antigens
Allergy and immunology
Papain
Enzymes
topic Antigens
Allergy and immunology
Papain
Enzymes
description Objective: To compare the enzyme activity of different presentations of papain solution to validate in-house preparations. Methods: Two papain solutions were prepared, and the third presentation was a commercial solution. Tests were carried out with samples of red cells typed as weak RhD. Results: In-house prepared papain solutions showed similar enzyme reactivity, and statistically no differences compared to the enzyme activity of the commercial solution. Conclusion: Evaluating the cost-benefit ratio, the in-house prepared papain solutions present more economic advantages, and can be incorporated into immunohematological routines as a way to cope with periods of financial crisis and cost-containment policies.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T07:30:36Z
2019-10-06T07:30:36Z
2019-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.31744/einstein_journal/2019AO4328
Einstein-sao Paulo. Sao Paulo Sp: Inst Israelita Ensino & Pesquisa Albert Einstein, v. 17, n. 2, 6 p., 2019.
1679-4508
http://hdl.handle.net/11449/186840
10.31744/einstein_journal/2019AO4328
S1679-45082019000200208
WOS:000483399500002
S1679-45082019000200208.pdf
url http://dx.doi.org/10.31744/einstein_journal/2019AO4328
http://hdl.handle.net/11449/186840
identifier_str_mv Einstein-sao Paulo. Sao Paulo Sp: Inst Israelita Ensino & Pesquisa Albert Einstein, v. 17, n. 2, 6 p., 2019.
1679-4508
10.31744/einstein_journal/2019AO4328
S1679-45082019000200208
WOS:000483399500002
S1679-45082019000200208.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Einstein-sao Paulo
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 6
application/pdf
dc.publisher.none.fl_str_mv Inst Israelita Ensino & Pesquisa Albert Einstein
publisher.none.fl_str_mv Inst Israelita Ensino & Pesquisa Albert Einstein
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1810021365420392448

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