Previous infection with Staphylococcus aureus strains attenuated experimental encephalomyelitis
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1471-2202-15-8 http://hdl.handle.net/11449/112633 |
Resumo: | Background: Bacterial superantigens are potent T cell activators that can activate T cells with specificity for antigens of the central nervous system (CNS). In this study, we compared the effect of two S. aureus strains on experimental autoimmune encephalomyelitis (EAE) development. C57BL/6 female mice were infected with S. aureus ATCC 51650, which produces toxic shock syndrome toxin 1 (TSST-1+) or S. aureus ATCC 43300, which does not produce toxins (TOX-). Three days later, the animals were subjected to EAE induction by immunization with myelin oligodendrocyte glycoprotein (MOG). The weight variation, disease incidence and clinical score were recorded daily. Cytokines and Foxp3+ regulatory T cells in the brain were evaluated during the acute disease phase. Cytokines and Foxp3+ regulatory T cells in the spleen and histopathological analysis of the CNS were assessed during the chronic stage.Results: Previous infection with both strains similarly decreased the clinical score; however, only the TSST-1+ strain clearly diminished inflammation in the CNS. The infections also modulated cytokine production in the spleen and CNS. Reduced production of IL-5 and IL-10 was detected in MOG-stimulated spleen cultures in the TOX- and TSST-1+ infected groups, respectively. In S. aureus stimulated cultures, there was an increased production of IFN-gamma and IL-10 in both infected groups and an increased level of IL-5 in the TSST-1+ group. CNS infiltrating cell cultures from previously infected mice produced less IL-17 in response to MOG and more IFN-gamma in response to S. aureus stimulation.Conclusions: These results indicated that both strains attenuated clinical EAE manifestations, but only TSST-1 clearly decreased CNS inflammation. |
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Repositório Institucional da UNESP |
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Previous infection with Staphylococcus aureus strains attenuated experimental encephalomyelitisS. aureusExperimental autoimmune encephalomyelitisToxic shock syndrome toxin 1Background: Bacterial superantigens are potent T cell activators that can activate T cells with specificity for antigens of the central nervous system (CNS). In this study, we compared the effect of two S. aureus strains on experimental autoimmune encephalomyelitis (EAE) development. C57BL/6 female mice were infected with S. aureus ATCC 51650, which produces toxic shock syndrome toxin 1 (TSST-1+) or S. aureus ATCC 43300, which does not produce toxins (TOX-). Three days later, the animals were subjected to EAE induction by immunization with myelin oligodendrocyte glycoprotein (MOG). The weight variation, disease incidence and clinical score were recorded daily. Cytokines and Foxp3+ regulatory T cells in the brain were evaluated during the acute disease phase. Cytokines and Foxp3+ regulatory T cells in the spleen and histopathological analysis of the CNS were assessed during the chronic stage.Results: Previous infection with both strains similarly decreased the clinical score; however, only the TSST-1+ strain clearly diminished inflammation in the CNS. The infections also modulated cytokine production in the spleen and CNS. Reduced production of IL-5 and IL-10 was detected in MOG-stimulated spleen cultures in the TOX- and TSST-1+ infected groups, respectively. In S. aureus stimulated cultures, there was an increased production of IFN-gamma and IL-10 in both infected groups and an increased level of IL-5 in the TSST-1+ group. CNS infiltrating cell cultures from previously infected mice produced less IL-17 in response to MOG and more IFN-gamma in response to S. aureus stimulation.Conclusions: These results indicated that both strains attenuated clinical EAE manifestations, but only TSST-1 clearly decreased CNS inflammation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, UNESP, Dept Microbiol & Immunol, Biosci Inst, BR-18618000 Botucatu, SP, BrazilFundacao Dr Amaral Carvalho, Lab Citometria Fluxo, Jau, SP, BrazilUniv Estadual Paulista, UNESP, Dept Microbiol & Immunol, Biosci Inst, BR-18618000 Botucatu, SP, BrazilFAPESP: 09/53175-7Biomed Central Ltd.Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Donega Franca, Thais Graziela [UNESP]Chiuso-Minicucci, Fernanda [UNESP]Goncalves Zorzella-Pezavento, Sofia Fernanda [UNESP]Watanabe Ishikawa, Larissa Lumi [UNESP]Rosa, Larissa Camargo da [UNESP]Colavite, Priscila Maria [UNESP]Marques, CamilaIkoma, Maura Rosane ValerioRibeiro de Souza da Cunha, Maria de Lourdes [UNESP]Sartori, Alexandrina [UNESP]2014-12-03T13:10:54Z2014-12-03T13:10:54Z2014-01-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://dx.doi.org/10.1186/1471-2202-15-8Bmc Neuroscience. London: Biomed Central Ltd, v. 15, 11 p., 2014.1471-2202http://hdl.handle.net/11449/11263310.1186/1471-2202-15-8WOS:000329611100001WOS000329611100001.pdf4977572416129527Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBmc Neuroscience2.1731,120info:eu-repo/semantics/openAccess2023-12-06T06:16:48Zoai:repositorio.unesp.br:11449/112633Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:36:35.948669Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Previous infection with Staphylococcus aureus strains attenuated experimental encephalomyelitis |
title |
Previous infection with Staphylococcus aureus strains attenuated experimental encephalomyelitis |
spellingShingle |
Previous infection with Staphylococcus aureus strains attenuated experimental encephalomyelitis Donega Franca, Thais Graziela [UNESP] S. aureus Experimental autoimmune encephalomyelitis Toxic shock syndrome toxin 1 |
title_short |
Previous infection with Staphylococcus aureus strains attenuated experimental encephalomyelitis |
title_full |
Previous infection with Staphylococcus aureus strains attenuated experimental encephalomyelitis |
title_fullStr |
Previous infection with Staphylococcus aureus strains attenuated experimental encephalomyelitis |
title_full_unstemmed |
Previous infection with Staphylococcus aureus strains attenuated experimental encephalomyelitis |
title_sort |
Previous infection with Staphylococcus aureus strains attenuated experimental encephalomyelitis |
author |
Donega Franca, Thais Graziela [UNESP] |
author_facet |
Donega Franca, Thais Graziela [UNESP] Chiuso-Minicucci, Fernanda [UNESP] Goncalves Zorzella-Pezavento, Sofia Fernanda [UNESP] Watanabe Ishikawa, Larissa Lumi [UNESP] Rosa, Larissa Camargo da [UNESP] Colavite, Priscila Maria [UNESP] Marques, Camila Ikoma, Maura Rosane Valerio Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP] Sartori, Alexandrina [UNESP] |
author_role |
author |
author2 |
Chiuso-Minicucci, Fernanda [UNESP] Goncalves Zorzella-Pezavento, Sofia Fernanda [UNESP] Watanabe Ishikawa, Larissa Lumi [UNESP] Rosa, Larissa Camargo da [UNESP] Colavite, Priscila Maria [UNESP] Marques, Camila Ikoma, Maura Rosane Valerio Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP] Sartori, Alexandrina [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Donega Franca, Thais Graziela [UNESP] Chiuso-Minicucci, Fernanda [UNESP] Goncalves Zorzella-Pezavento, Sofia Fernanda [UNESP] Watanabe Ishikawa, Larissa Lumi [UNESP] Rosa, Larissa Camargo da [UNESP] Colavite, Priscila Maria [UNESP] Marques, Camila Ikoma, Maura Rosane Valerio Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP] Sartori, Alexandrina [UNESP] |
dc.subject.por.fl_str_mv |
S. aureus Experimental autoimmune encephalomyelitis Toxic shock syndrome toxin 1 |
topic |
S. aureus Experimental autoimmune encephalomyelitis Toxic shock syndrome toxin 1 |
description |
Background: Bacterial superantigens are potent T cell activators that can activate T cells with specificity for antigens of the central nervous system (CNS). In this study, we compared the effect of two S. aureus strains on experimental autoimmune encephalomyelitis (EAE) development. C57BL/6 female mice were infected with S. aureus ATCC 51650, which produces toxic shock syndrome toxin 1 (TSST-1+) or S. aureus ATCC 43300, which does not produce toxins (TOX-). Three days later, the animals were subjected to EAE induction by immunization with myelin oligodendrocyte glycoprotein (MOG). The weight variation, disease incidence and clinical score were recorded daily. Cytokines and Foxp3+ regulatory T cells in the brain were evaluated during the acute disease phase. Cytokines and Foxp3+ regulatory T cells in the spleen and histopathological analysis of the CNS were assessed during the chronic stage.Results: Previous infection with both strains similarly decreased the clinical score; however, only the TSST-1+ strain clearly diminished inflammation in the CNS. The infections also modulated cytokine production in the spleen and CNS. Reduced production of IL-5 and IL-10 was detected in MOG-stimulated spleen cultures in the TOX- and TSST-1+ infected groups, respectively. In S. aureus stimulated cultures, there was an increased production of IFN-gamma and IL-10 in both infected groups and an increased level of IL-5 in the TSST-1+ group. CNS infiltrating cell cultures from previously infected mice produced less IL-17 in response to MOG and more IFN-gamma in response to S. aureus stimulation.Conclusions: These results indicated that both strains attenuated clinical EAE manifestations, but only TSST-1 clearly decreased CNS inflammation. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-03T13:10:54Z 2014-12-03T13:10:54Z 2014-01-09 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1471-2202-15-8 Bmc Neuroscience. London: Biomed Central Ltd, v. 15, 11 p., 2014. 1471-2202 http://hdl.handle.net/11449/112633 10.1186/1471-2202-15-8 WOS:000329611100001 WOS000329611100001.pdf 4977572416129527 |
url |
http://dx.doi.org/10.1186/1471-2202-15-8 http://hdl.handle.net/11449/112633 |
identifier_str_mv |
Bmc Neuroscience. London: Biomed Central Ltd, v. 15, 11 p., 2014. 1471-2202 10.1186/1471-2202-15-8 WOS:000329611100001 WOS000329611100001.pdf 4977572416129527 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bmc Neuroscience 2.173 1,120 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd. |
publisher.none.fl_str_mv |
Biomed Central Ltd. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129095471464448 |